3opy - Revision history

OCA at 10:35, 21 February 2024

2024-02-21T10:35:48Z

← Older revision		Revision as of 10:35, 21 February 2024
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	~~{{Large structure}}~~
	==Crystal structure of Pichia pastoris phosphofructokinase in the T-state==		==Crystal structure of Pichia pastoris phosphofructokinase in the T-state==
	<StructureSection load='3opy' size='340' side='right' caption='[[3opy]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='3opy' size='340' side='right'caption='[[3opy]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3opy]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pichia_pastoris Pichia~~ pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3OPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[3opy]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_pastoris_DSMZ_70382 Komagataella pastoris DSMZ 70382]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OPY FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=ATP:ADENOSINE-5~~-~~TRIPHOSPHATE'>ATP</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.05Å</td></tr>
	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11]~~ </~~span~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3opy OCA], [~~http~~://pdbe.org/3opy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3opy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3opy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3opy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3opy OCA], [https://pdbe.org/3opy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3opy RCSB], [https://www.ebi.ac.uk/pdbsum/3opy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3opy ProSAT]</span></td></tr>
	</table>		</table>
	~~{{Large structure}}~~
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/PFKA1_PICPA PFKA1_PICPA]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref> [[http://www.uniprot.org/uniprot/PFKA3_PICPA PFKA3_PICPA]] Structural subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Not required for catalytic activity. Fine-tunes allosteric regulation of the ATP-PFK by ATP, fructose 2,6-bisphosphate and AMP.<ref>PMID:17522059</ref> [[http://www.uniprot.org/uniprot/PFKA2_PICPA PFKA2_PICPA]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref>		[https://www.uniprot.org/uniprot/PFKA1_PICPA PFKA1_PICPA] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3opy ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3opy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Eukaryotic ATP-dependent 6-phosphofructokinases (Pfks) differ from their bacterial counterparts in a much more complex structural organization and allosteric regulation. Pichia pastoris Pfk (PpPfk) is, with approximately 1 MDa, the most complex and probably largest eukaryotic Pfk. We have determined the crystal structure of full-length PpPfk to 3.05 A resolution in the T state. PpPfk forms a (alphabetagamma)4 dodecamer of D2 symmetry with dimensions of 161 x 157 x 233 A mainly via interactions of the alpha chains. The N-terminal domains of the alpha and beta chains have folds that are distantly related to glyoxalase I, but the active sites are no longer functional. Interestingly, these domains located at the 2 distal ends of this protein along the long 2-fold axis form a (alphabeta)2 dimer as does the core Pfk domains; however, the domains are swapped across the tetramerization interface. In PpPfk, the unique gamma subunit participates in oligomerization of the alphabeta chains. This modulator protein was acquired from an ancient S-adenosylmethionine-dependent methyltransferase. The identification of novel ATP binding sites, which do not correspond to the bacterial catalytic or effector binding sites, point to marked structural and functional differences between bacterial and eukaryotic Pfks.-Strater, N., Marek, S., Kuettner, E. B., Kloos, M., Keim, A., Bruser, A., Kirchberger, J., Schoneberg, T. Molecular architecture and structural basis of allosteric regulation of eukaryotic phosphofructokinases.

	Molecular architecture and structural basis of allosteric regulation of eukaryotic phosphofructokinases.,Strater N, Marek S, Kuettner EB, Kloos M, Keim A, Bruser A, Kirchberger J, Schoneberg T FASEB J. 2010 Sep 10. PMID:20833871<ref>PMID:20833871</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3opy" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Phosphofructokinase ~~(PFK)~~\|Phosphofructokinase ~~(PFK)~~]]		*[[Phosphofructokinase 3D structures\|Phosphofructokinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~6-phosphofructokinase~~]]		[[Category: Komagataella pastoris DSMZ 70382]]
	[[Category: ~~Pichia pastoris~~]]		[[Category: Large Structures]]
	[[Category: Bruser, A]]		[[Category: Bruser A]]
	[[Category: Keim, A]]		[[Category: Keim A]]
	[[Category: Kirchberger, J]]		[[Category: Kirchberger J]]
	[[Category: Kloos, M]]		[[Category: Kloos M]]
	[[Category: Kuettner~~, E B~~]]		[[Category: Kuettner EB]]
	[[Category: Marek, S]]		[[Category: Marek S]]
	[[Category: Schoneberg, T]]		[[Category: Schoneberg T]]
	[[Category: Strater, N]]		[[Category: Strater N]]
	~~[[Category: 6-bisphosphate binding]]~~
	~~[[Category: Adp binding]]~~
	~~[[Category: Atp binding]]~~
	~~[[Category: Citrate binding]]~~
	~~[[Category: Fructose-2]]~~
	~~[[Category: Fructose-6-phosphate binding]]~~
	~~[[Category: Magnesium binding]]~~
	~~[[Category: Phosphofructokinase]]~~
	~~[[Category: Transferase~~]]

OCA at 10:43, 13 February 2019

2019-02-13T10:43:27Z

← Older revision		Revision as of 10:43, 13 February 2019
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	<StructureSection load='3opy' size='340' side='right' caption='[[3opy]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='3opy' size='340' side='right' caption='[[3opy]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3opy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Komagataella_pastoris Komagataella~~ pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[3opy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Pichia_pastoris Pichia pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OPY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/3opy_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/3opy_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
	[[Category: ~~Komagataella~~ pastoris]]		[[Category: Pichia pastoris]]
	[[Category: Bruser, A]]		[[Category: Bruser, A]]
	[[Category: Keim, A]]		[[Category: Keim, A]]

OCA at 11:17, 11 August 2016

2016-08-11T11:17:33Z

← Older revision		Revision as of 11:17, 11 August 2016
Line 1:		Line 1:
			{{Large structure}}
	==Crystal structure of Pichia pastoris phosphofructokinase in the T-state==		==Crystal structure of Pichia pastoris phosphofructokinase in the T-state==
	<StructureSection load='3opy' size='340' side='right' caption='[[3opy]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='3opy' size='340' side='right' caption='[[3opy]], [[Resolution\|resolution]] 3.05Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3opy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3opy RCSB], [http://www.ebi.ac.uk/pdbsum/3opy PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3opy OCA], [http://pdbe.org/3opy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3opy RCSB], [http://www.ebi.ac.uk/pdbsum/3opy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3opy ProSAT]</span></td></tr>
	</table>		</table>
			{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/~~Q8NJU8_PICPA Q8NJU8_PICPA~~]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref> [[http://www.uniprot.org/uniprot/~~A7MAS3_PICPA A7MAS3_PICPA~~]] Structural subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Not required for catalytic activity. Fine-tunes allosteric regulation of the ATP-PFK by ATP, fructose 2,6-bisphosphate and AMP.<ref>PMID:17522059</ref> [[http://www.uniprot.org/uniprot/~~Q8TGA0_PICPA Q8TGA0_PICPA~~]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref>		[[http://www.uniprot.org/uniprot/PFKA1_PICPA PFKA1_PICPA]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref> [[http://www.uniprot.org/uniprot/PFKA3_PICPA PFKA3_PICPA]] Structural subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Not required for catalytic activity. Fine-tunes allosteric regulation of the ATP-PFK by ATP, fructose 2,6-bisphosphate and AMP.<ref>PMID:17522059</ref> [[http://www.uniprot.org/uniprot/PFKA2_PICPA PFKA2_PICPA]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3opy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3opy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 14:50, 5 January 2015

2015-01-05T14:50:05Z

← Older revision		Revision as of 14:50, 5 January 2015
Line 1:		Line 1:
	~~{{Large~~ structure}}		==Crystal structure of Pichia pastoris phosphofructokinase in the T-state==
	~~{{STRUCTURE_3opy~~\| ~~PDB~~=3opy \| ~~SCENE=~~ }}		<StructureSection load='3opy' size='340' side='right' caption='[[3opy]], [[Resolution\|resolution]] 3.05Å' scene=''>
	===~~Crystal~~ structure of Pichia pastoris ~~phosphofructokinase~~ in the T-~~state===~~		== Structural highlights ==
	~~{{ABSTRACT_PUBMED_20833871}}~~		<table><tr><td colspan='2'>[[3opy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OPY FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3opy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3opy RCSB], [http://www.ebi.ac.uk/pdbsum/3opy PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q8NJU8_PICPA Q8NJU8_PICPA]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref> [[http://www.uniprot.org/uniprot/A7MAS3_PICPA A7MAS3_PICPA]] Structural subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Not required for catalytic activity. Fine-tunes allosteric regulation of the ATP-PFK by ATP, fructose 2,6-bisphosphate and AMP.<ref>PMID:17522059</ref> [[http://www.uniprot.org/uniprot/Q8TGA0_PICPA Q8TGA0_PICPA]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03184]<ref>PMID:12125050</ref>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/3opy_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Eukaryotic ATP-dependent 6-phosphofructokinases (Pfks) differ from their bacterial counterparts in a much more complex structural organization and allosteric regulation. Pichia pastoris Pfk (PpPfk) is, with approximately 1 MDa, the most complex and probably largest eukaryotic Pfk. We have determined the crystal structure of full-length PpPfk to 3.05 A resolution in the T state. PpPfk forms a (alphabetagamma)4 dodecamer of D2 symmetry with dimensions of 161 x 157 x 233 A mainly via interactions of the alpha chains. The N-terminal domains of the alpha and beta chains have folds that are distantly related to glyoxalase I, but the active sites are no longer functional. Interestingly, these domains located at the 2 distal ends of this protein along the long 2-fold axis form a (alphabeta)2 dimer as does the core Pfk domains; however, the domains are swapped across the tetramerization interface. In PpPfk, the unique gamma subunit participates in oligomerization of the alphabeta chains. This modulator protein was acquired from an ancient S-adenosylmethionine-dependent methyltransferase. The identification of novel ATP binding sites, which do not correspond to the bacterial catalytic or effector binding sites, point to marked structural and functional differences between bacterial and eukaryotic Pfks.-Strater, N., Marek, S., Kuettner, E. B., Kloos, M., Keim, A., Bruser, A., Kirchberger, J., Schoneberg, T. Molecular architecture and structural basis of allosteric regulation of eukaryotic phosphofructokinases.

	~~==About this Structure==~~		Molecular architecture and structural basis of allosteric regulation of eukaryotic phosphofructokinases.,Strater N, Marek S, Kuettner EB, Kloos M, Keim A, Bruser A, Kirchberger J, Schoneberg T FASEB J. 2010 Sep 10. PMID:20833871<ref>PMID:20833871</ref>
	~~[[3opy]] is a 12 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Komagataella_pastoris Komagataella pastoris]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3OPY OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	==See Also==		==See Also==
	*[[Phosphofructokinase (PFK)\|Phosphofructokinase (PFK)]]		*[[Phosphofructokinase (PFK)\|Phosphofructokinase (PFK)]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:020833871<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
	[[Category: Komagataella pastoris]]		[[Category: Komagataella pastoris]]
	[[Category: Bruser, A.]]		[[Category: Bruser, A]]
	[[Category: Keim, A.]]		[[Category: Keim, A]]
	[[Category: Kirchberger, J.]]		[[Category: Kirchberger, J]]
	[[Category: Kloos, M.]]		[[Category: Kloos, M]]
	[[Category: Kuettner, E B.]]		[[Category: Kuettner, E B]]
	[[Category: Marek, S.]]		[[Category: Marek, S]]
	[[Category: Schoneberg, T.]]		[[Category: Schoneberg, T]]
	[[Category: Strater, N.]]		[[Category: Strater, N]]
	[[Category: 6-bisphosphate binding]]		[[Category: 6-bisphosphate binding]]
	[[Category: Adp binding]]		[[Category: Adp binding]]

OCA at 08:31, 11 March 2013

2013-03-11T08:31:38Z

← Older revision		Revision as of 08:31, 11 March 2013
Line 1:		Line 1:
	~~[[Image:3opy.png\|left\|200px]]~~		{{Large structure}}

	{{STRUCTURE_3opy\| PDB=3opy \| SCENE= }}		{{STRUCTURE_3opy\| PDB=3opy \| SCENE= }}

	===Crystal structure of Pichia pastoris phosphofructokinase in the T-state===		===Crystal structure of Pichia pastoris phosphofructokinase in the T-state===

	{{ABSTRACT_PUBMED_20833871}}		{{ABSTRACT_PUBMED_20833871}}

OCA at 19:25, 20 October 2012

2012-10-20T19:25:44Z

← Older revision		Revision as of 19:25, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3opy]] is a 12 chain structure ~~of [[Phosphofructokinase (PFK)]]~~ with sequence from [http://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA].		[[3opy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA].

	==See Also==		==See Also==

OCA at 07:19, 27 July 2012

2012-07-27T07:19:52Z

← Older revision		Revision as of 07:19, 27 July 2012
Line 1:		Line 1:
	[[Image:3opy.png\|left\|200px]]		[[Image:3opy.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3opy", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3opy\| PDB=3opy \| SCENE= }}		{{STRUCTURE_3opy\| PDB=3opy \| SCENE= }}

	===Crystal structure of Pichia pastoris phosphofructokinase in the T-state===		===Crystal structure of Pichia pastoris phosphofructokinase in the T-state===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20833871}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 20833871 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_20833871}}		{{ABSTRACT_PUBMED_20833871}}

	==About this Structure==		==About this Structure==
	[[3opy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pichia_pastoris Pichia~~ pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA].		[[3opy]] is a 12 chain structure of [[Phosphofructokinase (PFK)]] with sequence from [http://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA].

			==See Also==
			*[[Phosphofructokinase (PFK)\|Phosphofructokinase (PFK)]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20833871~~</ref><references group="xtra"/>		<ref group="xtra">PMID:020833871</ref><references group="xtra"/>
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
	[[Category: ~~Pichia~~ pastoris]]		[[Category: Komagataella pastoris]]
	[[Category: Bruser, A.]]		[[Category: Bruser, A.]]
	[[Category: Keim, A.]]		[[Category: Keim, A.]]
Line 33:		Line 25:
	[[Category: Schoneberg, T.]]		[[Category: Schoneberg, T.]]
	[[Category: Strater, N.]]		[[Category: Strater, N.]]
			[[Category: 6-bisphosphate binding]]
			[[Category: Adp binding]]
			[[Category: Atp binding]]
			[[Category: Citrate binding]]
			[[Category: Fructose-2]]
			[[Category: Fructose-6-phosphate binding]]
			[[Category: Magnesium binding]]
			[[Category: Phosphofructokinase]]
			[[Category: Transferase]]

OCA: Protected "3opy" [edit=sysop:move=sysop]

2011-01-12T05:27:32Z

Protected "3opy" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:27, 12 January 2011
(No difference)

OCA at 05:27, 12 January 2011

2011-01-12T05:27:30Z

← Older revision		Revision as of 05:27, 12 January 2011
Line 1:		Line 1:
	~~{{Seed}}~~		[[Image:3opy.png\|left\|200px]]
	[[Image:3opy.~~jpg~~\|left\|200px]]

	<!--		<!--
Line 10:		Line 9:
	{{STRUCTURE_3opy\| PDB=3opy \| SCENE= }}		{{STRUCTURE_3opy\| PDB=3opy \| SCENE= }}

	===Crystal structure of ~~eukaryotic phosphofructokinase from yeast~~ Pichia pastoris ~~with a unique gamma subunit and glyoxalase subdomains~~ in ~~alpha and beta subunits~~===		===Crystal structure of Pichia pastoris phosphofructokinase in the T-state===


Line 20:		Line 19:

	==About this Structure==		==About this Structure==
	~~3OPY~~ is a 12 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Pichia_pastoris Pichia pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA].		[[3opy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Pichia_pastoris Pichia pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OPY OCA].

	==Reference==		==Reference==
Line 34:		Line 33:
	[[Category: Schoneberg, T.]]		[[Category: Schoneberg, T.]]
	[[Category: Strater, N.]]		[[Category: Strater, N.]]
	~~[[Category: 6-bisphosphate binding]]~~
	~~[[Category: Adp binding]]~~
	~~[[Category: Atp binding]]~~
	~~[[Category: Citrate binding]]~~
	~~[[Category: Fructose-2]]~~
	~~[[Category: Fructose-6-phosphate binding]]~~
	~~[[Category: Magnesium binding]]~~
	~~[[Category: Phosphofructokinase]]~~
	~~[[Category: Transferase]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 06:08:45 2010''~~

OCA at 02:59, 6 October 2010

2010-10-06T02:59:14Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3opy is ON HOLD  until Paper Publication
+<!--
-Authors: Strater, N., Marek, S., Kuettner, E.B., Kloos, M., Keim, A., Bruser, A., Kirchberger, J., Schoneberg, T.
+===Crystal structure of eukaryotic phosphofructokinase from yeast Pichia pastoris with a unique gamma subunit and glyoxalase subdomains in alpha and beta subunits===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:34:06 2010''
+<!--