3oth - Revision history

OCA at 02:14, 21 November 2024

2024-11-21T02:14:18Z

← Older revision		Revision as of 02:14, 21 November 2024
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [https://pdbe.org/3oth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [https://www.ebi.ac.uk/pdbsum/3oth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [https://pdbe.org/3oth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [https://www.ebi.ac.uk/pdbsum/3oth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	~~[https~~://~~www~~.~~uniprot~~.~~org~~/~~uniprot~~/~~Q8KNF2_MICEC Q8KNF2_MICEC]~~		== Publication Abstract from PubMed ==
			Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.

			Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:21987796</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3oth" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:18, 21 February 2024

2024-02-21T11:18:42Z

← Older revision		Revision as of 11:18, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3oth' size='340' side='right'caption='[[3oth]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3oth' size='340' side='right'caption='[[3oth]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_15837 Atcc 15837~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTH FirstGlance]. <br>		<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTH FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>~~, ~~<scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.301Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3otg\|3otg]], [[3oti\|3oti]], [[3d0r\|3d0r]], [[3d0q\|3d0q]]~~</~~div></td></tr>~~
	~~<tr id='gene'~~>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">calG1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [https://pdbe.org/3oth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [https://www.ebi.ac.uk/pdbsum/3oth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [https://pdbe.org/3oth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [https://www.ebi.ac.uk/pdbsum/3oth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/Q8KNF2_MICEC Q8KNF2_MICEC]
	Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.

	Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:~~21987796<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3oth" style="background-color:#fffaf0;"><~~/~~div>~~
	~~== References ==~~
	~~<references~~/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 15837]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Bingman, C A~~]]		[[Category: Micromonospora echinospora]]
	[[Category: ~~Structural genomic~~]]		[[Category: Bingman CA]]
	[[Category: Chang, A]]		[[Category: Chang A]]
	~~[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis~~]]		[[Category: Phillips Jr GN]]
	[[Category: Phillips~~, G N~~]]		[[Category: Singh S]]
	[[Category: Singh, S]]		[[Category: Thorson JS]]
	[[Category: Thorson~~, J S]]~~
	~~[[Category: Calicheamicin]]~~
	~~[[Category: Cesg]]~~
	~~[[Category: Enzyme discovery for natural product biosynthesis]]~~
	~~[[Category: Glycosyltransferase]]~~
	~~[[Category: Gt-b fold]]~~
	~~[[Category: Natpro]]~~
	~~[[Category: PSI, Protein structure initiative]]~~
	~~[[Category: Tdp]]~~
	~~[[Category: Transferase-antibiotic complex~~]]

OCA at 10:56, 18 May 2022

2022-05-18T10:56:34Z

← Older revision		Revision as of 10:56, 18 May 2022
Line 1:		Line 1:

	==Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form==		==Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form==
	<StructureSection load='3oth' size='340' side='right' caption='[[3oth]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3oth' size='340' side='right'caption='[[3oth]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_15837 Atcc 15837]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3OTH FirstGlance]. <br>		<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_15837 Atcc 15837]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3otg\|3otg]], [[3oti\|3oti]], [[3d0r\|3d0r]], [[3d0q\|3d0q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3otg\|3otg]], [[3oti\|3oti]], [[3d0r\|3d0r]], [[3d0q\|3d0q]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">calG1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">calG1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [~~http~~://pdbe.org/3oth PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3oth PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [https://pdbe.org/3oth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [https://www.ebi.ac.uk/pdbsum/3oth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 15837]]		[[Category: Atcc 15837]]
			[[Category: Large Structures]]
	[[Category: Bingman, C A]]		[[Category: Bingman, C A]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 08:50, 8 November 2017

2017-11-08T08:50:17Z

← Older revision		Revision as of 08:50, 8 November 2017
Line 33:		Line 33:
	[[Category: Calicheamicin]]		[[Category: Calicheamicin]]
	[[Category: Cesg]]		[[Category: Cesg]]
			[[Category: Enzyme discovery for natural product biosynthesis]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Gt-b fold]]		[[Category: Gt-b fold]]
			[[Category: Natpro]]
	[[Category: PSI, Protein structure initiative]]		[[Category: PSI, Protein structure initiative]]
	[[Category: Tdp]]		[[Category: Tdp]]
	[[Category: Transferase-antibiotic complex]]		[[Category: Transferase-antibiotic complex]]

OCA at 17:28, 4 August 2016

2016-08-04T17:28:51Z

← Older revision		Revision as of 17:28, 4 August 2016
Line 1:		Line 1:

	==Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form==		==Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form==
	<StructureSection load='3oth' size='340' side='right' caption='[[3oth]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3oth' size='340' side='right' caption='[[3oth]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Micromonospora_echinospora Micromonospora echinospora~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OTH FirstGlance]. <br>		<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15837 Atcc 15837]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OTH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3otg\|3otg]], [[3oti\|3oti]], [[3d0r\|3d0r]], [[3d0q\|3d0q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3otg\|3otg]], [[3oti\|3oti]], [[3d0r\|3d0r]], [[3d0q\|3d0q]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">calG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ~~Micromonospora echinospora~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">calG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [http://www.ebi.ac.uk/pdbsum/3oth PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [http://pdbe.org/3oth PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [http://www.ebi.ac.uk/pdbsum/3oth PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3oth" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Micromonospora echinospora~~]]		[[Category: Atcc 15837]]
	[[Category: Bingman, C A]]		[[Category: Bingman, C A]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 21:46, 3 January 2015

2015-01-03T21:46:16Z

← Older revision		Revision as of 21:46, 3 January 2015
Line 1:		Line 1:
	[[~~Image~~:~~3oth~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form==
			<StructureSection load='3oth' size='340' side='right' caption='[[3oth]], [[Resolution\|resolution]] 2.30Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OTH FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
			<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3otg\|3otg]], [[3oti\|3oti]], [[3d0r\|3d0r]], [[3d0q\|3d0q]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">calG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 Micromonospora echinospora])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [http://www.ebi.ac.uk/pdbsum/3oth PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.

	~~{{STRUCTURE_3oth\| PDB=3oth \| SCENE= }}~~		Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:21987796</ref>

	~~===Crystal Structure of CalG1, Calicheamicin Glycostyltransferase~~, ~~TDP and calicheamicin alpha3I bound form===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_21987796}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[3oth]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Micromonospora_echinospora Micromonospora echinospora]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:021987796<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Micromonospora echinospora]]		[[Category: Micromonospora echinospora]]
	[[Category: Bingman, C A.]]		[[Category: Bingman, C A]]
	[[Category: ~~CESG, Center for Eukaryotic~~ Structural ~~Genomics.~~]]		[[Category: Structural genomic]]
	[[Category: Chang, A.]]		[[Category: Chang, A]]
	[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis.]]		[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
	[[Category: Phillips, G N.]]		[[Category: Phillips, G N]]
	[[Category: Singh, S.]]		[[Category: Singh, S]]
	[[Category: Thorson, J S.]]		[[Category: Thorson, J S]]
	[[Category: Calicheamicin]]		[[Category: Calicheamicin]]
	~~[[Category: Center for eukaryotic structural genomic]]~~
	[[Category: Cesg]]		[[Category: Cesg]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Gt-b fold]]		[[Category: Gt-b fold]]
	[[Category: Protein structure initiative]]		[[Category: PSI, Protein structure initiative]]
	~~[[Category: Psi-2]]~~
	~~[[Category: Structural genomic~~]]
	[[Category: Tdp]]		[[Category: Tdp]]
	[[Category: Transferase-antibiotic complex]]		[[Category: Transferase-antibiotic complex]]

OCA at 04:30, 8 August 2012

2012-08-08T04:30:39Z

← Older revision		Revision as of 04:30, 8 August 2012
Line 1:		Line 1:
	[[Image:3oth.png\|left\|200px]]		[[Image:3oth.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3oth", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3oth\| PDB=3oth \| SCENE= }}		{{STRUCTURE_3oth\| PDB=3oth \| SCENE= }}

	===Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form===		===Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21987796}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 21987796 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_21987796}}		{{ABSTRACT_PUBMED_21987796}}

Line 27:		Line 16:
	[[Category: CESG, Center for Eukaryotic Structural Genomics.]]		[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
	[[Category: Chang, A.]]		[[Category: Chang, A.]]
			[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis.]]
	[[Category: Phillips, G N.]]		[[Category: Phillips, G N.]]
	[[Category: Singh, S.]]		[[Category: Singh, S.]]

OCA: Protected "3oth" [edit=sysop:move=sysop]

2011-11-02T07:29:14Z

Protected "3oth" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:29, 2 November 2011
(No difference)

OCA at 07:29, 2 November 2011

2011-11-02T07:29:12Z

← Older revision		Revision as of 07:29, 2 November 2011
Line 1:		Line 1:
	~~{{Seed}}~~		[[Image:3oth.png\|left\|200px]]
	[[Image:3oth.~~jpg~~\|left\|200px]]

	<!--		<!--
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	===Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form===		===Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_21987796}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 21987796 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_21987796}}

	==About this Structure==		==About this Structure==
	~~3OTH~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA].		[[3oth]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA].

			==Reference==
			<ref group="xtra">PMID:021987796</ref><references group="xtra"/>
	[[Category: Micromonospora echinospora]]		[[Category: Micromonospora echinospora]]
	[[Category: Bingman, C A.]]		[[Category: Bingman, C A.]]
Line 32:		Line 40:
	[[Category: Tdp]]		[[Category: Tdp]]
	[[Category: Transferase-antibiotic complex]]		[[Category: Transferase-antibiotic complex]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:43:57 2010''~~

OCA at 05:31, 15 December 2010

2010-12-15T05:31:38Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3oth is ON HOLD
+<!--
-Authors: Chang, A., Singh, S., Bingman, C.A., Thorson, J.S., Phillips Jr., G.N., Center for Eukaryotic Structural Genomics (CESG)
+===Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:34:47 2010''
+==About this Structure==