3phf - Revision history

OCA at 06:38, 27 November 2024

2024-11-27T06:38:40Z

← Older revision		Revision as of 06:38, 27 November 2024
Line 8:		Line 8:
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/~~GH_EBVB9 GH_EBVB9~~] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:~~11021994~~</ref>		[https://www.uniprot.org/uniprot/GL_EBVB9 GL_EBVB9] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The Epstein-Barr virus (EBV) is a gamma-herpesvirus that infects B cells and epithelial cells and that has been linked to malignancies in both cell types in vivo. EBV, like other herpesviruses, has three glycoproteins, glycoprotein B (gB), gH, and gL, that form the core membrane fusion machinery mediating viral penetration into the cell. The gH and gL proteins associate to form a heterodimeric complex, which is necessary for efficient membrane fusion and also implicated in direct binding to epithelial cell receptors required for viral entry. To gain insight into the mechanistic role of gH/gL, we determined the crystal structure of the EBV gH/gL complex. The structure is comprised of four domains organized along the longest axis of the molecule. Comparisons with homologous HSV-2 gH/gL and partial pseudorabies virus gH structures support the domain boundaries determined for the EBV gH/gL structure and illustrate significant differences in interdomain packing angles. The gL subunit and N-terminal residues of gH form a globular domain at one end of the structure, implicated in interactions with gB and activation of membrane fusion. The C-terminal domain of gH, proximal to the viral membrane, is also implicated in membrane fusion. The gH/gL structure locates an integrin binding motif, implicated in epithelial cell entry, on a prominent loop in the central region of the structure. Multiple regions of gH/gL, including its two extreme ends, are functionally important, consistent with the multiple roles of gH/gL in EBV entry.

			Crystal structure of the Epstein-Barr virus (EBV) glycoprotein H/glycoprotein L (gH/gL) complex.,Matsuura H, Kirschner AN, Longnecker R, Jardetzky TS Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22641-6. Epub 2010 Dec 13. PMID:21149717<ref>PMID:21149717</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3phf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:31, 1 March 2024

2024-03-01T13:31:36Z

← Older revision		Revision as of 13:31, 1 March 2024
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	<StructureSection load='3phf' size='340' side='right'caption='[[3phf]], [[Resolution\|resolution]] 3.58Å' scene=''>		<StructureSection load='3phf' size='340' side='right'caption='[[3phf]], [[Resolution\|resolution]] 3.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3phf]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ebvg Ebvg~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PHF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3phf]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_gammaherpesvirus_4 Human gammaherpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PHF FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3m1c\|3m1c]]</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.58Å</td></tr>
	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">BXLF2, gH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 EBVG]), BKRF2, GL ([https://www.ncbi.nlm.nih~~.~~gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10376 EBVG])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [https://pdbe.org/3phf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [https://www.ebi.ac.uk/pdbsum/3phf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3phf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [https://pdbe.org/3phf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [https://www.ebi.ac.uk/pdbsum/3phf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3phf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/GH_EBVB9 GH_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:11021994</ref> [[https://www.uniprot.org/uniprot/GL_EBVB9 GL_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells (By similarity).		[https://www.uniprot.org/uniprot/GH_EBVB9 GH_EBVB9] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:11021994</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The Epstein-Barr virus (EBV) is a gamma-herpesvirus that infects B cells and epithelial cells and that has been linked to malignancies in both cell types in vivo. EBV, like other herpesviruses, has three glycoproteins, glycoprotein B (gB), gH, and gL, that form the core membrane fusion machinery mediating viral penetration into the cell. The gH and gL proteins associate to form a heterodimeric complex, which is necessary for efficient membrane fusion and also implicated in direct binding to epithelial cell receptors required for viral entry. To gain insight into the mechanistic role of gH/gL, we determined the crystal structure of the EBV gH/gL complex. The structure is comprised of four domains organized along the longest axis of the molecule. Comparisons with homologous HSV-2 gH/gL and partial pseudorabies virus gH structures support the domain boundaries determined for the EBV gH/gL structure and illustrate significant differences in interdomain packing angles. The gL subunit and N-terminal residues of gH form a globular domain at one end of the structure, implicated in interactions with gB and activation of membrane fusion. The C-terminal domain of gH, proximal to the viral membrane, is also implicated in membrane fusion. The gH/gL structure locates an integrin binding motif, implicated in epithelial cell entry, on a prominent loop in the central region of the structure. Multiple regions of gH/gL, including its two extreme ends, are functionally important, consistent with the multiple roles of gH/gL in EBV entry.

	Crystal structure of the Epstein-Barr virus (EBV) glycoprotein H/glycoprotein L (gH/gL) complex.,Matsuura H, Kirschner AN, Longnecker R, Jardetzky TS Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22641-6. Epub 2010 Dec 13. PMID:21149717<ref>PMID:21149717</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3phf" style="background-color:#fffaf0;"></div~~>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Ebvg~~]]		[[Category: Human gammaherpesvirus 4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jardetzky~~, T S~~]]		[[Category: Jardetzky TS]]
	[[Category: Kirschner~~, A N~~]]		[[Category: Kirschner AN]]
	[[Category: Matsuura, H]]		[[Category: Matsuura H]]
	~~[[Category: Glycoprotein]]~~
	~~[[Category: Membrane fusion]]~~
	~~[[Category: Viral protein]]~~
	~~[[Category: Virus entry~~]]

OCA at 08:39, 25 May 2022

2022-05-25T08:39:48Z

← Older revision		Revision as of 08:39, 25 May 2022
Line 1:		Line 1:
	~~{{Large structure}}~~
	==Crystal Structure of the Epstein-Barr virus gH and gL complex==		==Crystal Structure of the Epstein-Barr virus gH and gL complex==
	<StructureSection load='3phf' size='340' side='right' caption='[[3phf]], [[Resolution\|resolution]] 3.58Å' scene=''>		<StructureSection load='3phf' size='340' side='right'caption='[[3phf]], [[Resolution\|resolution]] 3.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3phf]] is a 32 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ebvg Ebvg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PHF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3phf]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebvg Ebvg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PHF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m1c\|3m1c]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3m1c\|3m1c]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BXLF2, gH ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 EBVG]), BKRF2, GL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 EBVG])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BXLF2, gH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 EBVG]), BKRF2, GL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 EBVG])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [~~http~~://pdbe.org/3phf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3phf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3phf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [https://pdbe.org/3phf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [https://www.ebi.ac.uk/pdbsum/3phf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3phf ProSAT]</span></td></tr>
	</table>		</table>
	~~{{Large structure}}~~
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/GH_EBVB9 GH_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:11021994</ref> [[~~http~~://www.uniprot.org/uniprot/GL_EBVB9 GL_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells (By similarity).		[[https://www.uniprot.org/uniprot/GH_EBVB9 GH_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:11021994</ref> [[https://www.uniprot.org/uniprot/GL_EBVB9 GL_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Ebvg]]		[[Category: Ebvg]]
			[[Category: Large Structures]]
	[[Category: Jardetzky, T S]]		[[Category: Jardetzky, T S]]
	[[Category: Kirschner, A N]]		[[Category: Kirschner, A N]]

OCA at 22:41, 5 August 2016

2016-08-05T22:41:45Z

← Older revision		Revision as of 22:41, 5 August 2016
Line 1:		Line 1:
			{{Large structure}}
	==Crystal Structure of the Epstein-Barr virus gH and gL complex==		==Crystal Structure of the Epstein-Barr virus gH and gL complex==
	<StructureSection load='3phf' size='340' side='right' caption='[[3phf]], [[Resolution\|resolution]] 3.58Å' scene=''>		<StructureSection load='3phf' size='340' side='right' caption='[[3phf]], [[Resolution\|resolution]] 3.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3phf]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Human_herpesvirus_4 Human herpesvirus 4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PHF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3phf]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebvg Ebvg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PHF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m1c\|3m1c]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m1c\|3m1c]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BXLF2, gH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 ~~Human herpesvirus 4~~]), BKRF2, GL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 ~~Human herpesvirus 4~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BXLF2, gH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 EBVG]), BKRF2, GL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 EBVG])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [http://www.ebi.ac.uk/pdbsum/3phf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [http://pdbe.org/3phf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [http://www.ebi.ac.uk/pdbsum/3phf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3phf ProSAT]</span></td></tr>
	</table>		</table>
			{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GH_EBVB9 GH_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:11021994</ref> [[http://www.uniprot.org/uniprot/GL_EBVB9 GL_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells (By similarity).		[[http://www.uniprot.org/uniprot/GH_EBVB9 GH_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:11021994</ref> [[http://www.uniprot.org/uniprot/GL_EBVB9 GL_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells (By similarity).
Line 17:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3phf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human herpesvirus 4~~]]		[[Category: Ebvg]]
	[[Category: Jardetzky, T S]]		[[Category: Jardetzky, T S]]
	[[Category: Kirschner, A N]]		[[Category: Kirschner, A N]]

OCA at 22:33, 25 December 2014

2014-12-25T22:33:04Z

← Older revision		Revision as of 22:33, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [http://www.ebi.ac.uk/pdbsum/3phf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [http://www.ebi.ac.uk/pdbsum/3phf PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/GH_EBVB9 GH_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:11021994</ref> [[http://www.uniprot.org/uniprot/GL_EBVB9 GL_EBVB9]] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:29, 18 December 2014

2014-12-18T08:29:46Z

← Older revision		Revision as of 08:29, 18 December 2014
Line 1:		Line 1:
	~~{{Large~~ structure}}		==Crystal Structure of the Epstein-Barr virus gH and gL complex==
	~~{{STRUCTURE_3phf~~\| ~~PDB~~=3phf ~~\| SCENE~~= }}		<StructureSection load='3phf' size='340' side='right' caption='[[3phf]], [[Resolution\|resolution]] 3.58Å' scene=''>
	===~~Crystal Structure of the~~ Epstein-Barr virus gH and gL complex~~===~~		== Structural highlights ==
	~~{{ABSTRACT_PUBMED_21149717}}~~		<table><tr><td colspan='2'>[[3phf]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PHF FirstGlance]. <br>
			</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m1c\|3m1c]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BXLF2, gH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 Human herpesvirus 4]), BKRF2, GL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10376 Human herpesvirus 4])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3phf RCSB], [http://www.ebi.ac.uk/pdbsum/3phf PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The Epstein-Barr virus (EBV) is a gamma-herpesvirus that infects B cells and epithelial cells and that has been linked to malignancies in both cell types in vivo. EBV, like other herpesviruses, has three glycoproteins, glycoprotein B (gB), gH, and gL, that form the core membrane fusion machinery mediating viral penetration into the cell. The gH and gL proteins associate to form a heterodimeric complex, which is necessary for efficient membrane fusion and also implicated in direct binding to epithelial cell receptors required for viral entry. To gain insight into the mechanistic role of gH/gL, we determined the crystal structure of the EBV gH/gL complex. The structure is comprised of four domains organized along the longest axis of the molecule. Comparisons with homologous HSV-2 gH/gL and partial pseudorabies virus gH structures support the domain boundaries determined for the EBV gH/gL structure and illustrate significant differences in interdomain packing angles. The gL subunit and N-terminal residues of gH form a globular domain at one end of the structure, implicated in interactions with gB and activation of membrane fusion. The C-terminal domain of gH, proximal to the viral membrane, is also implicated in membrane fusion. The gH/gL structure locates an integrin binding motif, implicated in epithelial cell entry, on a prominent loop in the central region of the structure. Multiple regions of gH/gL, including its two extreme ends, are functionally important, consistent with the multiple roles of gH/gL in EBV entry.

	~~==About this Structure==~~		Crystal structure of the Epstein-Barr virus (EBV) glycoprotein H/glycoprotein L (gH/gL) complex.,Matsuura H, Kirschner AN, Longnecker R, Jardetzky TS Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22641-6. Epub 2010 Dec 13. PMID:21149717<ref>PMID:21149717</ref>
	~~[[3phf]] is a 32 chain~~ structure ~~with sequence from [http:~~//en.~~wikipedia~~.~~org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3PHF OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	<~~ref group="xtra">PMID:021149717<~~/~~ref~~><~~references group="xtra"~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Human herpesvirus 4]]		[[Category: Human herpesvirus 4]]
	[[Category: Jardetzky, T S.]]		[[Category: Jardetzky, T S]]
	[[Category: Kirschner, A N.]]		[[Category: Kirschner, A N]]
	[[Category: Matsuura, H.]]		[[Category: Matsuura, H]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Membrane fusion]]		[[Category: Membrane fusion]]
	[[Category: Viral protein]]		[[Category: Viral protein]]
	[[Category: Virus entry]]		[[Category: Virus entry]]

OCA at 08:01, 11 March 2013

2013-03-11T08:01:26Z

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-[[Image:3phf.png|left|200px]]
+{{Large structure}}
 {{STRUCTURE_3phf|  PDB=3phf  |  SCENE=  }}
 ===Crystal Structure of the Epstein-Barr virus gH and gL complex===
 {{ABSTRACT_PUBMED_21149717}}

OCA at 06:17, 1 February 2012

2012-02-01T06:17:29Z

← Older revision		Revision as of 06:17, 1 February 2012
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	==Reference==		==Reference==
	<ref group="xtra">PMID:~~21149717~~</ref><references group="xtra"/>		<ref group="xtra">PMID:021149717</ref><references group="xtra"/>
	[[Category: Human herpesvirus 4]]		[[Category: Human herpesvirus 4]]
	[[Category: Jardetzky, T S.]]		[[Category: Jardetzky, T S.]]
	[[Category: Kirschner, A N.]]		[[Category: Kirschner, A N.]]
	[[Category: Matsuura, H.]]		[[Category: Matsuura, H.]]
			[[Category: Glycoprotein]]
			[[Category: Membrane fusion]]
			[[Category: Viral protein]]
			[[Category: Virus entry]]

OCA: Protected "3phf" [edit=sysop:move=sysop]

2011-01-12T05:18:07Z

Protected "3phf" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:18, 12 January 2011
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OCA at 05:18, 12 January 2011

2011-01-12T05:18:04Z

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-The entry 3phf is ON HOLD  until Paper Publication
+<!--
-Authors: Matsuura, H., Kirschner, A.N., Jardetzky, T.S.
+===Crystal Structure of the Epstein-Barr virus gH and gL complex===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 22 09:36:04 2010''
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