3pps - Revision history

OCA at 08:06, 9 October 2024

2024-10-09T08:06:37Z

← Older revision		Revision as of 08:06, 9 October 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/F6N9E7_9PEZI F6N9E7_9PEZI]		[https://www.uniprot.org/uniprot/F6N9E7_9PEZI F6N9E7_9PEZI]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Laccases are copper-containing enzymes used in various applications such as textile bleaching. Several crystal structures of laccases from fungi and bacteria are available, but ascomycete-types of fungal laccases have been rather unexplored and to date only the crystal structure of Melanocarpus albomyces laccase (MaL) has been published. We have now solved the crystal structure of another asco-laccase from Thielavia arenaria (TaLcc1) at 2.5 A resolution. The loops near the T1 copper, forming the substrate binding pockets of the two asco-laccases, differ to some extent and include the amino acid thought to be responsible for catalytic proton transfer, which is Asp in TaLcc1 instead of Glu observed in MaL. In addition, the crystal structure of TaLcc1 does not have a chloride attached to the T2 copper as observed in the crystal structure of MaL. The unique feature of the TaLcc1 and MaL compared to other laccases structures is that in both structures the processed C-terminus blocks the T3 solvent channel leading towards the trinuclear centre, suggesting a common functional role for this conserved "C-terminal plug". We propose that the ascomycete laccases utilize the C-terminal carboxylic group in proton transfer processes, similarly as has been suggested for the Glu498 in the CotA-laccase from Bacillus subtilis (BsL). The crystal structure of TaLcc1 also shows the formation of a similar weak homodimer as observed for MaL that may determine the properties of these asco-laccases at high protein concentrations.

			Crystal structure of an ascomycete fungal laccase from Thielavia arenaria: Common structural features of asco-laccases.,Kallio JP, Gasparetti C, Andberg M, Boer H, Koivula A, Kruus K, Rouvinen J, Hakulinen N FEBS J. 2011 Apr 29. doi: 10.1111/j.1742-4658.2011.08146.x. PMID:21535408<ref>PMID:21535408</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3pps" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Laccase 3D structures\|Laccase 3D structures]]		*[[Laccase 3D structures\|Laccase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:33, 1 March 2024

2024-03-01T13:33:06Z

← Older revision		Revision as of 13:33, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3pps' size='340' side='right'caption='[[3pps]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3pps' size='340' side='right'caption='[[3pps]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Canariomyces_arenarius Canariomyces arenarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PPS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=~~NAG~~:~~N-ACETYL~~-D-~~GLUCOSAMINE~~'>~~NAG~~</scene>, <scene name='pdbligand=~~OXY~~:~~OXYGEN+MOLECULE~~'>~~OXY~~</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA-D-MANNOSE~~'>~~MAN~~</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Laccase Laccase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [https://pdbe.org/3pps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [https://www.ebi.ac.uk/pdbsum/3pps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pps ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [https://pdbe.org/3pps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [https://www.ebi.ac.uk/pdbsum/3pps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pps ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/F6N9E7_9PEZI F6N9E7_9PEZI]
	Laccases are copper-containing enzymes used in various applications such as textile bleaching. Several crystal structures of laccases from fungi and bacteria are available, but ascomycete-types of fungal laccases have been rather unexplored and to date only the crystal structure of Melanocarpus albomyces laccase (MaL) has been published. We have now solved the crystal structure of another asco-laccase from Thielavia arenaria (TaLcc1) at 2.5 A resolution. The loops near the T1 copper, forming the substrate binding pockets of the two asco-laccases, differ to some extent and include the amino acid thought to be responsible for catalytic proton transfer, which is Asp in TaLcc1 instead of Glu observed in MaL. In addition, the crystal structure of TaLcc1 does not have a chloride attached to the T2 copper as observed in the crystal structure of MaL. The unique feature of the TaLcc1 and MaL compared to other laccases structures is that in both structures the processed C-terminus blocks the T3 solvent channel leading towards the trinuclear centre, suggesting a common functional role for this conserved "C-terminal plug". We propose that the ascomycete laccases utilize the C-terminal carboxylic group in proton transfer processes, similarly as has been suggested for the Glu498 in the CotA-laccase from Bacillus subtilis (BsL). The crystal structure of TaLcc1 also shows the formation of a similar weak homodimer as observed for MaL that may determine the properties of these asco-laccases at high protein concentrations.

	~~Crystal structure of an ascomycete fungal laccase from Thielavia arenaria~~: ~~Common structural features of asco-laccases.,Kallio JP, Gasparetti C, Andberg M, Boer H, Koivula A, Kruus K, Rouvinen J, Hakulinen N FEBS J. 2011 Apr 29. doi: 10.1111~~/~~j.1742-4658.2011.08146.x. PMID:21535408<ref>PMID:21535408<~~/~~ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 3pps" style="background-color:#fffaf0;"><~~/~~div>~~

	==See Also==		==See Also==
	*[[Laccase 3D structures\|Laccase 3D structures]]		*[[Laccase 3D structures\|Laccase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Laccase~~]]		[[Category: Canariomyces arenarius]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hakulinen, N]]		[[Category: Hakulinen N]]
	[[Category: Kallio~~, J P~~]]		[[Category: Kallio JP]]
	[[Category: Rouvinen, J]]		[[Category: Rouvinen J]]
	~~[[Category: Beta barrel]]~~
	~~[[Category: Copper binding]]~~
	~~[[Category: Cupredoxin fold]]~~
	~~[[Category: Oxidoreductase~~]]

OCA at 08:50, 25 May 2022

2022-05-25T08:50:08Z

← Older revision		Revision as of 08:50, 25 May 2022
Line 1:		Line 1:

	==Crystal structure of an ascomycete fungal laccase from Thielavia arenaria==		==Crystal structure of an ascomycete fungal laccase from Thielavia arenaria==
	<StructureSection load='3pps' size='340' side='right' caption='[[3pps]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3pps' size='340' side='right'caption='[[3pps]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Cbs_507.74 Cbs 507.74]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PPS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA~~-D-~~MANNOSE~~'>~~MAN~~</scene>, <scene name='pdbligand=~~NAG~~:~~N-ACETYL-D-GLUCOSAMINE~~'>~~NAG~~</scene>, <scene name='pdbligand=~~OXY~~:~~OXYGEN+MOLECULE~~'>~~OXY~~</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Laccase Laccase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Laccase Laccase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [~~http~~://pdbe.org/3pps PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pps PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pps ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [https://pdbe.org/3pps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [https://www.ebi.ac.uk/pdbsum/3pps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pps ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 3pps" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3pps" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Laccase 3D structures\|Laccase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Cbs 507 74]]~~
	[[Category: Laccase]]		[[Category: Laccase]]
			[[Category: Large Structures]]
	[[Category: Hakulinen, N]]		[[Category: Hakulinen, N]]
	[[Category: Kallio, J P]]		[[Category: Kallio, J P]]

OCA at 10:29, 13 February 2019

2019-02-13T10:29:58Z

← Older revision		Revision as of 10:29, 13 February 2019
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_507.74 Cbs 507.74]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_507.74 Cbs 507.74]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PPS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=~~NAG~~:~~N-ACETYL~~-D-~~GLUCOSAMINE~~'>~~NAG~~</scene>, <scene name='pdbligand=~~OXY~~:~~OXYGEN+MOLECULE~~'>~~OXY~~</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA-D-MANNOSE~~'>~~MAN~~</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>

OCA at 23:16, 4 August 2016

2016-08-04T23:16:08Z

← Older revision		Revision as of 23:16, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of an ascomycete fungal laccase from Thielavia arenaria==		==Crystal structure of an ascomycete fungal laccase from Thielavia arenaria==
	<StructureSection load='3pps' size='340' side='right' caption='[[3pps]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3pps' size='340' side='right' caption='[[3pps]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thielavia_arenaria Thielavia arenaria~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_507.74 Cbs 507.74]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PPS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [http://www.ebi.ac.uk/pdbsum/3pps PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [http://pdbe.org/3pps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [http://www.ebi.ac.uk/pdbsum/3pps PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pps ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3pps" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Cbs 507 74]]
	[[Category: Laccase]]		[[Category: Laccase]]
	~~[[Category: Thielavia arenaria]]~~
	[[Category: Hakulinen, N]]		[[Category: Hakulinen, N]]
	[[Category: Kallio, J P]]		[[Category: Kallio, J P]]

OCA at 23:00, 3 January 2015

2015-01-03T23:00:43Z

← Older revision		Revision as of 23:00, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thielavia_arenaria Thielavia arenaria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thielavia_arenaria Thielavia arenaria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PPS FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [http://www.ebi.ac.uk/pdbsum/3pps PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [http://www.ebi.ac.uk/pdbsum/3pps PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Crystal structure of an ascomycete fungal laccase from Thielavia arenaria: Common structural features of asco-laccases.,Kallio JP, Gasparetti C, Andberg M, Boer H, Koivula A, Kruus K, Rouvinen J, Hakulinen N FEBS J. 2011 Apr 29. doi: 10.1111/j.1742-4658.2011.08146.x. PMID:21535408<ref>PMID:21535408</ref>		Crystal structure of an ascomycete fungal laccase from Thielavia arenaria: Common structural features of asco-laccases.,Kallio JP, Gasparetti C, Andberg M, Boer H, Koivula A, Kruus K, Rouvinen J, Hakulinen N FEBS J. 2011 Apr 29. doi: 10.1111/j.1742-4658.2011.08146.x. PMID:21535408<ref>PMID:21535408</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 22:
	[[Category: Laccase]]		[[Category: Laccase]]
	[[Category: Thielavia arenaria]]		[[Category: Thielavia arenaria]]
	[[Category: Hakulinen, N.]]		[[Category: Hakulinen, N]]
	[[Category: Kallio, J P.]]		[[Category: Kallio, J P]]
	[[Category: Rouvinen, J.]]		[[Category: Rouvinen, J]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Copper binding]]		[[Category: Copper binding]]
	[[Category: Cupredoxin fold]]		[[Category: Cupredoxin fold]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 05:20, 4 June 2014

2014-06-04T05:20:39Z

← Older revision		Revision as of 05:20, 4 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~3pps~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of an ascomycete fungal laccase from Thielavia arenaria==
			<StructureSection load='3pps' size='340' side='right' caption='[[3pps]], [[Resolution\|resolution]] 2.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3pps]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thielavia_arenaria Thielavia arenaria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PPS FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gw0\|1gw0]], [[2ih8\|2ih8]], [[2ih9\|2ih9]], [[2q9o\|2q9o]], [[3dkh\|3dkh]], [[3fu7\|3fu7]], [[3fu8\|3fu8]], [[3fu9\|3fu9]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pps RCSB], [http://www.ebi.ac.uk/pdbsum/3pps PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Laccases are copper-containing enzymes used in various applications such as textile bleaching. Several crystal structures of laccases from fungi and bacteria are available, but ascomycete-types of fungal laccases have been rather unexplored and to date only the crystal structure of Melanocarpus albomyces laccase (MaL) has been published. We have now solved the crystal structure of another asco-laccase from Thielavia arenaria (TaLcc1) at 2.5 A resolution. The loops near the T1 copper, forming the substrate binding pockets of the two asco-laccases, differ to some extent and include the amino acid thought to be responsible for catalytic proton transfer, which is Asp in TaLcc1 instead of Glu observed in MaL. In addition, the crystal structure of TaLcc1 does not have a chloride attached to the T2 copper as observed in the crystal structure of MaL. The unique feature of the TaLcc1 and MaL compared to other laccases structures is that in both structures the processed C-terminus blocks the T3 solvent channel leading towards the trinuclear centre, suggesting a common functional role for this conserved "C-terminal plug". We propose that the ascomycete laccases utilize the C-terminal carboxylic group in proton transfer processes, similarly as has been suggested for the Glu498 in the CotA-laccase from Bacillus subtilis (BsL). The crystal structure of TaLcc1 also shows the formation of a similar weak homodimer as observed for MaL that may determine the properties of these asco-laccases at high protein concentrations.

	<!--		Crystal structure of an ascomycete fungal laccase from Thielavia arenaria: Common structural features of asco-laccases.,Kallio JP, Gasparetti C, Andberg M, Boer H, Koivula A, Kruus K, Rouvinen J, Hakulinen N FEBS J. 2011 Apr 29. doi: 10.1111/j.1742-4658.2011.08146.x. PMID:21535408<ref>PMID:21535408</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3pps"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3pps\| PDB=3pps \| SCENE= }}~~

	~~===Crystal structure~~ of ~~an ascomycete fungal laccase from Thielavia arenaria===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	~~<!--~~		<references/>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21535408}}, adds the Publication Abstract to~~ the ~~page~~		__TOC__
	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21535408 is the PubMed ID number~~.		</StructureSection>
	-->
	~~{{ABSTRACT_PUBMED_21535408}}~~

	~~==About this Structure==~~
	~~[[3pps]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Thielavia_arenaria Thielavia arenaria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPS OCA].~~

	==~~Reference~~==
	<~~ref group="xtra">PMID:021535408<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Laccase]]		[[Category: Laccase]]
	[[Category: Thielavia arenaria]]		[[Category: Thielavia arenaria]]

OCA at 05:56, 24 August 2011

2011-08-24T05:56:49Z

← Older revision		Revision as of 05:56, 24 August 2011
Line 1:		Line 1:
	[[Image:3pps.~~jpg~~\|left\|200px]]		[[Image:3pps.png\|left\|200px]]

	<!--		<!--
Line 28:		Line 28:
	[[Category: Kallio, J P.]]		[[Category: Kallio, J P.]]
	[[Category: Rouvinen, J.]]		[[Category: Rouvinen, J.]]
			[[Category: Beta barrel]]
			[[Category: Copper binding]]
			[[Category: Cupredoxin fold]]
			[[Category: Oxidoreductase]]

OCA at 05:32, 18 May 2011

2011-05-18T05:32:01Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3pps.jpg|left|200px]]
-The entry 3pps is ON HOLD  until Paper Publication
+<!--
-Authors: Kallio, J.P., Rouvinen, J., Hakulinen, N.
+===Crystal structure of an ascomycete fungal laccase from Thielavia arenaria===
-Description: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria

OCA: Protected "3pps" [edit=sysop:move=sysop]

2011-03-02T06:35:56Z

Protected "3pps" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:35, 2 March 2011
(No difference)