3pr7 - Revision history

OCA at 10:42, 21 February 2024

2024-02-21T10:42:43Z

← Older revision		Revision as of 10:42, 21 February 2024
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	<StructureSection load='3pr7' size='340' side='right'caption='[[3pr7]], [[Resolution\|resolution]] 2.94Å' scene=''>		<StructureSection load='3pr7' size='340' side='right'caption='[[3pr7]], [[Resolution\|resolution]] 2.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"mikrokkokus_catarrhalis"_(sic)_frosch_and_kolle_in_flugge_1896 "mikrokkokus~~ catarrhalis~~" (sic) frosch and kolle in flugge 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3ntn\|3ntn]]</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.94Å</td></tr>
	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">uspA1 ([https://www.ncbi~~.~~nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=480 "Mikrokkokus catarrhalis" (sic) Frosch and Kolle in Flugge 1896])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [https://pdbe.org/3pr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [https://www.ebi.ac.uk/pdbsum/3pr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pr7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [https://pdbe.org/3pr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [https://www.ebi.ac.uk/pdbsum/3pr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pr7 ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	=~~= Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/Q9XD56_MORCA Q9XD56_MORCA]
	Bacterial cell surfaces are commonly decorated with a layer formed from multiple copies of adhesin proteins whose binding interactions initiate colonization and infection processes. In this study, we investigate the physical deformability of the UspA1 adhesin protein from Moraxella catarrhalis, a causative agent of middle-ear infections in humans. UspA1 binds a range of extracellular proteins including fibronectin, and the epithelial cellular receptor carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1). Electron microscopy indicates that unliganded UspA1 is densely packed at, and extends about 800 A from, the Moraxella surface. Using a modified atomic force microscope, we show that the adhesive properties and thickness of the UspA1 layer at the cell surface varies on addition of either fibronectin or CEACAM1. This in situ analysis is then correlated with the molecular structure of UspA1. To provide an overall model for UspA1, we have determined crystal structures for two N-terminal fragments which are then combined with a previous structure of the CEACAM1-binding site. We show that the UspA1-fibronectin complex is formed between UspA1 head region and the 13th type-III domain of fibronectin and, using X-ray scattering, that the complex involves an angular association between these two proteins. In combination with a previous study, which showed that the CEACAM1-UspA1 complex is distinctively bent in solution, we correlate these observations on isolated fragments of UspA1 with its in situ response on the cell surface. This study therefore provides a rare direct demonstration of protein conformational change at the cell surface.

	Correlation of in situ mechanosensitive responses of the Moraxella catarrhalis adhesin UspA1 with fibronectin and receptor CEACAM1 binding.,Agnew C, Borodina E, Zaccai NR, Conners R, Burton NM, Vicary JA, Cole DK, Antognozzi M, Virji M, Brady RL Proc Natl Acad Sci U S A. 2011 Aug 29. PMID:~~21876142<ref>PMID:21876142<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 3pr7" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references~~/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Agnew, C R.J~~]]		[[Category: Moraxella catarrhalis]]
	[[Category: ~~Brady, R L~~]]		[[Category: Agnew CRJ]]
	[[Category: ~~Conners, R~~]]		[[Category: Brady RL]]
	[[Category: ~~Zaccai, N~~ R]]		[[Category: Conners R]]
	[[Category: ~~Adhesin]]~~		[[Category: Zaccai NR]]
	~~[[Category: Beta-roll and coiled-coil]]~~
	~~[[Category: Extracellular]]~~
	~~[[Category: Membrane protein~~]]

OCA at 08:51, 25 May 2022

2022-05-25T08:51:41Z

← Older revision		Revision as of 08:51, 25 May 2022
Line 1:		Line 1:

	==Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1==		==Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1==
	<StructureSection load='3pr7' size='340' side='right' caption='[[3pr7]], [[Resolution\|resolution]] 2.94Å' scene=''>		<StructureSection load='3pr7' size='340' side='right'caption='[[3pr7]], [[Resolution\|resolution]] 2.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"mikrokkokus_catarrhalis"_(sic)_frosch_and_kolle_in_flugge_1896 "mikrokkokus catarrhalis" (sic) frosch and kolle in flugge 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"mikrokkokus_catarrhalis"_(sic)_frosch_and_kolle_in_flugge_1896 "mikrokkokus catarrhalis" (sic) frosch and kolle in flugge 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ntn\|3ntn]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ntn\|3ntn]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uspA1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=480 "Mikrokkokus catarrhalis" (sic) Frosch and Kolle in Flugge 1896])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uspA1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=480 "Mikrokkokus catarrhalis" (sic) Frosch and Kolle in Flugge 1896])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [~~http~~://pdbe.org/3pr7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pr7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pr7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [https://pdbe.org/3pr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [https://www.ebi.ac.uk/pdbsum/3pr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pr7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Agnew, C R.J]]		[[Category: Agnew, C R.J]]
	[[Category: Brady, R L]]		[[Category: Brady, R L]]

OCA at 15:51, 4 August 2016

2016-08-04T15:51:51Z

← Older revision		Revision as of 15:51, 4 August 2016
Line 1:		Line 1:

	==Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1==		==Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1==
	<StructureSection load='3pr7' size='340' side='right' caption='[[3pr7]], [[Resolution\|resolution]] 2.94Å' scene=''>		<StructureSection load='3pr7' size='340' side='right' caption='[[3pr7]], [[Resolution\|resolution]] 2.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Moraxella_catarrhalis Moraxella~~ catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"mikrokkokus_catarrhalis"_(sic)_frosch_and_kolle_in_flugge_1896 "mikrokkokus catarrhalis" (sic) frosch and kolle in flugge 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ntn\|3ntn]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ntn\|3ntn]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uspA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=480 ~~Moraxella~~ catarrhalis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uspA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=480 "Mikrokkokus catarrhalis" (sic) Frosch and Kolle in Flugge 1896])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [http://www.ebi.ac.uk/pdbsum/3pr7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [http://pdbe.org/3pr7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [http://www.ebi.ac.uk/pdbsum/3pr7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pr7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3pr7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Moraxella catarrhalis]]~~
	[[Category: Agnew, C R.J]]		[[Category: Agnew, C R.J]]
	[[Category: Brady, R L]]		[[Category: Brady, R L]]

OCA at 22:45, 3 January 2015

2015-01-03T22:45:14Z

← Older revision		Revision as of 22:45, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ntn\|3ntn]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ntn\|3ntn]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uspA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=480 Moraxella catarrhalis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uspA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=480 Moraxella catarrhalis])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [http://www.ebi.ac.uk/pdbsum/3pr7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [http://www.ebi.ac.uk/pdbsum/3pr7 PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Correlation of in situ mechanosensitive responses of the Moraxella catarrhalis adhesin UspA1 with fibronectin and receptor CEACAM1 binding.,Agnew C, Borodina E, Zaccai NR, Conners R, Burton NM, Vicary JA, Cole DK, Antognozzi M, Virji M, Brady RL Proc Natl Acad Sci U S A. 2011 Aug 29. PMID:21876142<ref>PMID:21876142</ref>		Correlation of in situ mechanosensitive responses of the Moraxella catarrhalis adhesin UspA1 with fibronectin and receptor CEACAM1 binding.,Agnew C, Borodina E, Zaccai NR, Conners R, Burton NM, Vicary JA, Cole DK, Antognozzi M, Virji M, Brady RL Proc Natl Acad Sci U S A. 2011 Aug 29. PMID:21876142<ref>PMID:21876142</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Moraxella catarrhalis]]		[[Category: Moraxella catarrhalis]]
	[[Category: Agnew, C R.J.]]		[[Category: Agnew, C R.J]]
	[[Category: Brady, R L.]]		[[Category: Brady, R L]]
	[[Category: Conners, R.]]		[[Category: Conners, R]]
	[[Category: Zaccai, N R.]]		[[Category: Zaccai, N R]]
	[[Category: Adhesin]]		[[Category: Adhesin]]
	[[Category: Beta-roll and coiled-coil]]		[[Category: Beta-roll and coiled-coil]]
	[[Category: Extracellular]]		[[Category: Extracellular]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 05:27, 4 June 2014

2014-06-04T05:27:46Z

← Older revision		Revision as of 05:27, 4 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~3pr7~~.~~png~~\|~~left~~\|~~200px~~]]		==Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1==
			<StructureSection load='3pr7' size='340' side='right' caption='[[3pr7]], [[Resolution\|resolution]] 2.94Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3pr7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PR7 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ntn\|3ntn]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uspA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=480 Moraxella catarrhalis])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pr7 RCSB], [http://www.ebi.ac.uk/pdbsum/3pr7 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Bacterial cell surfaces are commonly decorated with a layer formed from multiple copies of adhesin proteins whose binding interactions initiate colonization and infection processes. In this study, we investigate the physical deformability of the UspA1 adhesin protein from Moraxella catarrhalis, a causative agent of middle-ear infections in humans. UspA1 binds a range of extracellular proteins including fibronectin, and the epithelial cellular receptor carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1). Electron microscopy indicates that unliganded UspA1 is densely packed at, and extends about 800 A from, the Moraxella surface. Using a modified atomic force microscope, we show that the adhesive properties and thickness of the UspA1 layer at the cell surface varies on addition of either fibronectin or CEACAM1. This in situ analysis is then correlated with the molecular structure of UspA1. To provide an overall model for UspA1, we have determined crystal structures for two N-terminal fragments which are then combined with a previous structure of the CEACAM1-binding site. We show that the UspA1-fibronectin complex is formed between UspA1 head region and the 13th type-III domain of fibronectin and, using X-ray scattering, that the complex involves an angular association between these two proteins. In combination with a previous study, which showed that the CEACAM1-UspA1 complex is distinctively bent in solution, we correlate these observations on isolated fragments of UspA1 with its in situ response on the cell surface. This study therefore provides a rare direct demonstration of protein conformational change at the cell surface.

	~~<!--~~		Correlation of in situ mechanosensitive responses of the Moraxella catarrhalis adhesin UspA1 with fibronectin and receptor CEACAM1 binding.,Agnew C, Borodina E, Zaccai NR, Conners R, Burton NM, Vicary JA, Cole DK, Antognozzi M, Virji M, Brady RL Proc Natl Acad Sci U S A. 2011 Aug 29. PMID:21876142<ref>PMID:21876142</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3pr7"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3pr7\| PDB=3pr7 \| SCENE= }}~~

	~~===Multi-functional and mechanosensitive receptor binding activity~~ of the ~~Moraxella catarrhalis adhesin UspA1===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	~~<!--~~		<references/>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21876142}}, adds the Publication Abstract to the page~~		__TOC__
	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21876142 is the PubMed ID number~~.		</StructureSection>
	-->
	~~{{ABSTRACT_PUBMED_21876142}}~~

	~~==About this Structure==~~
	~~[[3pr7]] is a 3 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA].~~

	==~~Reference~~==
	<~~ref group="xtra">PMID:021876142<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Moraxella catarrhalis]]		[[Category: Moraxella catarrhalis]]
	[[Category: Agnew, C R.J.]]		[[Category: Agnew, C R.J.]]

OCA at 05:44, 14 September 2011

2011-09-14T05:44:59Z

← Older revision		Revision as of 05:44, 14 September 2011
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	[[Image:3pr7.~~jpg~~\|left\|200px]]		[[Image:3pr7.png\|left\|200px]]

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	===Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1===		===Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1===


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			The line below this paragraph, {{ABSTRACT_PUBMED_21876142}}, adds the Publication Abstract to the page
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			{{ABSTRACT_PUBMED_21876142}}

	==About this Structure==		==About this Structure==
	[[3pr7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA].		[[3pr7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PR7 OCA].

			==Reference==
			<ref group="xtra">PMID:021876142</ref><references group="xtra"/>
	[[Category: Moraxella catarrhalis]]		[[Category: Moraxella catarrhalis]]
	[[Category: Agnew, C R.J.]]		[[Category: Agnew, C R.J.]]

OCA at 06:18, 7 September 2011

2011-09-07T06:18:19Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3pr7.jpg|left|200px]]
-The entry 3pr7 is ON HOLD  until Paper Publication
+<!--
-Authors: Agnew, C.R.J., Zaccai, N.R., Conners, R., Brady, R.L.
+===Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1===
-Description: Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1

OCA: Protected "3pr7" [edit=sysop:move=sysop]

2011-03-23T06:42:13Z

Protected "3pr7" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:42, 23 March 2011
(No difference)

OCA at 06:42, 23 March 2011

2011-03-23T06:42:12Z

← Older revision		Revision as of 06:42, 23 March 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3pr7 is ON HOLD		The entry 3pr7 is ON HOLD until Paper Publication

	Authors: Agnew, C.R.J., Zaccai, N.R., Conners, R., Brady, R.L.		Authors: Agnew, C.R.J., Zaccai, N.R., Conners, R., Brady, R.L.

	Description: Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1		Description: Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 8 11:33:13 2010''~~

OCA: New page: '''Unreleased structure''' The entry 3pr7 is ON HOLD Authors: Agnew, C.R.J., Zaccai, N.R., Conners, R., Brady, R.L. Description: Multi-functional and mechanosensitive receptor binding ...

2010-12-08T08:21:09Z

New page: '''Unreleased structure''' The entry 3pr7 is ON HOLD Authors: Agnew, C.R.J., Zaccai, N.R., Conners, R., Brady, R.L. Description: Multi-functional and mechanosensitive receptor binding ...

New page

'''Unreleased structure'''

The entry 3pr7 is ON HOLD

Authors: Agnew, C.R.J., Zaccai, N.R., Conners, R., Brady, R.L.

Description: Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 8 11:33:13 2010''