3pse - Revision history

OCA at 14:57, 20 September 2023

2023-09-20T14:57:12Z

← Older revision		Revision as of 14:57, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crimean-Congo_hemorrhagic_fever_orthonairovirus Crimean-Congo hemorrhagic fever orthonairovirus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crimean-Congo_hemorrhagic_fever_orthonairovirus Crimean-Congo hemorrhagic fever orthonairovirus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [https://pdbe.org/3pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [https://www.ebi.ac.uk/pdbsum/3pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [https://pdbe.org/3pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [https://www.ebi.ac.uk/pdbsum/3pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pse ProSAT]</span></td></tr>
	</table>		</table>

OCA at 05:48, 31 May 2023

2023-05-31T05:48:57Z

← Older revision		Revision as of 05:48, 31 May 2023
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	<StructureSection load='3pse' size='340' side='right'caption='[[3pse]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3pse' size='340' side='right'caption='[[3pse]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ ] and [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crimean-Congo_hemorrhagic_fever_orthonairovirus Crimean-Congo hemorrhagic fever orthonairovirus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pt2\|3pt2]]</div></td></tr>~~
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISG15, G1P2, UCRP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [https://pdbe.org/3pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [https://www.ebi.ac.uk/pdbsum/3pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [https://pdbe.org/3pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [https://www.ebi.ac.uk/pdbsum/3pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/~~ISG15_HUMAN ISG15_HUMAN~~]~~] Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN~~-~~beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin~~, ~~differing in substrate specificity~~ and ~~interaction with ligating enzymes~~. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-~~1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils~~ and ~~activates them to induce release~~ of ~~eosinophil chemotactic factors. May serve as a trans-acting binding factor directing~~ the ~~association of ligated target proteins to intermediate filaments~~. ~~May also be involved in autocrine, paracrine~~ and ~~endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes~~ and macrophages. Seems to display antiviral activity during viral infections.<ref>PMID:1373138</ref> <ref>PMID:7526157</ref> <ref>PMID:8550581</ref> <ref>PMID:2005397</ref> <ref>PMID:16254333</ref> <ref>PMID:16009940</ref> In response to IFN-tau secreted by the conceptus, may ~~ligate to and~~ regulate ~~proteins involved in the release of prostaglandin F2-alpha (PGF),~~ and ~~thus prevent lysis of the corpus luteum and maintain the pregnancy (By similarity)~~.~~<ref>PMID:1373138</ref> <ref>PMID:7526157</ref> <ref>PMID:8550581</ref> <ref>PMID:2005397</ref> <ref>PMID:16254333</ref> <ref>PMID:16009940</ref>~~		[https://www.uniprot.org/uniprot/L_CCHFI L_CCHFI] Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Crimean-Congo hemorrhagic fever orthonairovirus]]
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bacik~~, J P~~]]		[[Category: Bacik JP]]
	[[Category: Frias-Staheli, N]]		[[Category: Frias-Staheli N]]
	[[Category: Garcia-Sastre, A]]		[[Category: Garcia-Sastre A]]
	[[Category: James~~, T W~~]]		[[Category: James TW]]
	[[Category: Mark~~, B L]]~~		[[Category: Mark BL]]
	~~[[Category: 3-aminopropane]]~~
	~~[[Category: Crimean-congo hemorrhagic fever virus]]~~
	~~[[Category: Hydrolase-protein binding complex]]~~
	~~[[Category: Intein-mediated ligation]]~~
	~~[[Category: Viral deubiquitinase~~]]

OCA at 08:53, 25 May 2022

2022-05-25T08:53:08Z

← Older revision		Revision as of 08:53, 25 May 2022
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	==Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)==		==Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)==
	<StructureSection load='3pse' size='340' side='right' caption='[[3pse]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3pse' size='340' side='right'caption='[[3pse]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cchfv Cchfv~~] and [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ ] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pt2\|3pt2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pt2\|3pt2]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISG15, G1P2, UCRP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISG15, G1P2, UCRP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [~~http~~://pdbe.org/3pse PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pse PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [https://pdbe.org/3pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [https://www.ebi.ac.uk/pdbsum/3pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/ISG15_HUMAN ISG15_HUMAN]] Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.<ref>PMID:1373138</ref> <ref>PMID:7526157</ref> <ref>PMID:8550581</ref> <ref>PMID:2005397</ref> <ref>PMID:16254333</ref> <ref>PMID:16009940</ref> In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (By similarity).<ref>PMID:1373138</ref> <ref>PMID:7526157</ref> <ref>PMID:8550581</ref> <ref>PMID:2005397</ref> <ref>PMID:16254333</ref> <ref>PMID:16009940</ref>		[[https://www.uniprot.org/uniprot/ISG15_HUMAN ISG15_HUMAN]] Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.<ref>PMID:1373138</ref> <ref>PMID:7526157</ref> <ref>PMID:8550581</ref> <ref>PMID:2005397</ref> <ref>PMID:16254333</ref> <ref>PMID:16009940</ref> In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (By similarity).<ref>PMID:1373138</ref> <ref>PMID:7526157</ref> <ref>PMID:8550581</ref> <ref>PMID:2005397</ref> <ref>PMID:16254333</ref> <ref>PMID:16009940</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[RNA polymerase\|RNA polymerase]]		*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Cchfv]]~~
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Bacik, J P]]		[[Category: Bacik, J P]]
	[[Category: Frias-Staheli, N]]		[[Category: Frias-Staheli, N]]

OCA at 07:17, 4 August 2016

2016-08-04T07:17:04Z

← Older revision		Revision as of 07:17, 4 August 2016
Line 1:		Line 1:

	==Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)==		==Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)==
	<StructureSection load='3pse' size='340' side='right' caption='[[3pse]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3pse' size='340' side='right' caption='[[3pse]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus~~] and [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cchfv Cchfv] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pt2\|3pt2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pt2\|3pt2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISG15, G1P2, UCRP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISG15, G1P2, UCRP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [http://www.ebi.ac.uk/pdbsum/3pse PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [http://pdbe.org/3pse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [http://www.ebi.ac.uk/pdbsum/3pse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3pse" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Crimean-congo hemorrhagic fever virus~~]]		[[Category: Cchfv]]
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Bacik, J P]]		[[Category: Bacik, J P]]
	[[Category: Frias-Staheli, N]]		[[Category: Frias-Staheli, N]]
Line 33:		Line 35:
	[[Category: Mark, B L]]		[[Category: Mark, B L]]
	[[Category: 3-aminopropane]]		[[Category: 3-aminopropane]]
			[[Category: Crimean-congo hemorrhagic fever virus]]
	[[Category: Hydrolase-protein binding complex]]		[[Category: Hydrolase-protein binding complex]]
	[[Category: Intein-mediated ligation]]		[[Category: Intein-mediated ligation]]
	[[Category: Viral deubiquitinase]]		[[Category: Viral deubiquitinase]]

OCA at 06:42, 24 December 2014

2014-12-24T06:42:03Z

← Older revision		Revision as of 06:42, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [http://www.ebi.ac.uk/pdbsum/3pse PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [http://www.ebi.ac.uk/pdbsum/3pse PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/ISG15_HUMAN ISG15_HUMAN]] Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.<ref>PMID:1373138</ref> <ref>PMID:7526157</ref> <ref>PMID:8550581</ref> <ref>PMID:2005397</ref> <ref>PMID:16254333</ref> <ref>PMID:16009940</ref> In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (By similarity).<ref>PMID:1373138</ref> <ref>PMID:7526157</ref> <ref>PMID:8550581</ref> <ref>PMID:2005397</ref> <ref>PMID:16254333</ref> <ref>PMID:16009940</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:38, 9 December 2014

2014-12-09T10:38:12Z

← Older revision		Revision as of 10:38, 9 December 2014
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	[[~~Image~~:~~3pse~~.~~png~~\|~~left~~\|~~200px~~]]		==Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)==
			<StructureSection load='3pse' size='340' side='right' caption='[[3pse]], [[Resolution\|resolution]] 2.30Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3pse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PSE FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pt2\|3pt2]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISG15, G1P2, UCRP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pse RCSB], [http://www.ebi.ac.uk/pdbsum/3pse PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The attachment of ubiquitin (Ub) and the Ub-like (Ubl) molecule interferon-stimulated gene 15 (ISG15) to cellular proteins mediates important innate antiviral responses. Ovarian tumor (OTU) domain proteases from nairoviruses and arteriviruses were recently found to remove these molecules from host proteins, which inhibits Ub and ISG15-dependent antiviral pathways. This contrasts with the Ub-specific activity of known eukaryotic OTU-domain proteases. Here we describe crystal structures of a viral OTU domain from the highly pathogenic Crimean-Congo haemorrhagic fever virus (CCHFV) bound to Ub and to ISG15 at 2.5-A and 2.3-A resolution, respectively. The complexes provide a unique structural example of ISG15 bound to another protein and reveal the molecular mechanism of an ISG15 cross-reactive deubiquitinase. To accommodate structural differences between Ub and ISG15, the viral protease binds the beta-grasp folds of Ub and C-terminal Ub-like domain of ISG15 in an orientation that is rotated nearly 75 degrees with respect to that observed for Ub bound to a representative eukaryotic OTU domain from yeast. Distinct structural determinants necessary for binding either substrate were identified and allowed the reengineering of the viral OTU protease into enzymes with increased substrate specificity, either for Ub or for ISG15. Our findings now provide the basis to determine in vivo the relative contributions of deubiquitination and deISGylation to viral immune evasion tactics, and a structural template of a promiscuous deubiquitinase from a haemorrhagic fever virus that can be targeted for inhibition using small-molecule-based strategies.

	~~{{STRUCTURE_3pse\| PDB=3pse \| SCENE= }}~~		Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease.,James TW, Frias-Staheli N, Bacik JP, Levingston Macleod JM, Khajehpour M, Garcia-Sastre A, Mark BL Proc Natl Acad Sci U S A. 2011 Jan 18. PMID:21245344<ref>PMID:21245344</ref>

	~~===Structure~~ of ~~a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_21245344}}~~

	~~==About this Structure==~~
	~~[[3pse]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus] and [http://en~~.~~wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:/~~/~~oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA].~~

	==See Also==		==See Also==
	*[[RNA polymerase\|RNA polymerase]]		*[[RNA polymerase\|RNA polymerase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:021245344<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Crimean-congo hemorrhagic fever virus]]		[[Category: Crimean-congo hemorrhagic fever virus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Bacik, J P.]]		[[Category: Bacik, J P]]
	[[Category: Frias-Staheli, N.]]		[[Category: Frias-Staheli, N]]
	[[Category: Garcia-Sastre, A.]]		[[Category: Garcia-Sastre, A]]
	[[Category: James, T W.]]		[[Category: James, T W]]
	[[Category: Mark, B L.]]		[[Category: Mark, B L]]
	[[Category: 3-aminopropane]]		[[Category: 3-aminopropane]]
	~~[[Category: Crimean-congo hemorrhagic fever virus]]~~
	[[Category: Hydrolase-protein binding complex]]		[[Category: Hydrolase-protein binding complex]]
	[[Category: Intein-mediated ligation]]		[[Category: Intein-mediated ligation]]
	[[Category: Viral deubiquitinase]]		[[Category: Viral deubiquitinase]]

OCA at 05:15, 20 October 2012

2012-10-20T05:15:10Z

← Older revision		Revision as of 05:15, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[3pse]] is a 2 chain structure ~~of [[RNA polymerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA].		[[3pse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA].

	==See Also==		==See Also==

OCA at 09:27, 25 July 2012

2012-07-25T09:27:54Z

← Older revision		Revision as of 09:27, 25 July 2012
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	[[Image:3pse.png\|left\|200px]]		[[Image:3pse.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3pse", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3pse\| PDB=3pse \| SCENE= }}		{{STRUCTURE_3pse\| PDB=3pse \| SCENE= }}

	===Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)===		===Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21245344}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 21245344 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_21245344}}		{{ABSTRACT_PUBMED_21245344}}

	==About this Structure==		==About this Structure==
	[[3pse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA].		[[3pse]] is a 2 chain structure of [[RNA polymerase]] with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA].

			==See Also==
			*[[RNA polymerase\|RNA polymerase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~21245344~~</ref><references group="xtra"/>		<ref group="xtra">PMID:021245344</ref><references group="xtra"/>
	[[Category: Crimean-congo hemorrhagic fever virus]]		[[Category: Crimean-congo hemorrhagic fever virus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
Line 30:		Line 22:
	[[Category: James, T W.]]		[[Category: James, T W.]]
	[[Category: Mark, B L.]]		[[Category: Mark, B L.]]
			[[Category: 3-aminopropane]]
			[[Category: Crimean-congo hemorrhagic fever virus]]
			[[Category: Hydrolase-protein binding complex]]
			[[Category: Intein-mediated ligation]]
			[[Category: Viral deubiquitinase]]

OCA at 05:53, 2 February 2011

2011-02-02T05:53:29Z

← Older revision		Revision as of 05:53, 2 February 2011
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	[[Image:3pse.~~jpg~~\|left\|200px]]		[[Image:3pse.png\|left\|200px]]

	<!--		<!--
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	===Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)===		===Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_21245344}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 21245344 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_21245344}}

	==About this Structure==		==About this Structure==
	[[3pse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA].		[[3pse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSE OCA].

			==Reference==
			<ref group="xtra">PMID:21245344</ref><references group="xtra"/>
	[[Category: Crimean-congo hemorrhagic fever virus]]		[[Category: Crimean-congo hemorrhagic fever virus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA: Protected "3pse" [edit=sysop:move=sysop]

2011-01-19T06:28:20Z

Protected "3pse" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:28, 19 January 2011
(No difference)