3qvf - Revision history

OCA at 10:09, 13 August 2026

2026-08-13T10:09:15Z

@@ Line 10: / Line 10: @@
 == Function ==
 [https://www.uniprot.org/uniprot/Q56187_STRWE Q56187_STRWE]
 __TOC__
 </StructureSection>

OCA at 12:00, 14 March 2024

2024-03-14T12:00:09Z

← Older revision		Revision as of 12:00, 14 March 2024
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	<StructureSection load='3qvf' size='340' side='right'caption='[[3qvf]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3qvf' size='340' side='right'caption='[[3qvf]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"actinomyces_wedmorensis"_milard_and_burr_1926 "actinomyces~~ wedmorensis~~" milard and burr 1926~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QVF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qun\|3qun]], [[3quo\|3quo]], [[3qur\|3qur]], [[3d40\|3d40]], [[3d41\|3d41]], [[3qvh\|3qvh]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fomA, fosfomycin resistance kinase ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 "Actinomyces wedmorensis" Milard and Burr 1926])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [https://pdbe.org/3qvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [https://www.ebi.ac.uk/pdbsum/3qvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [https://pdbe.org/3qvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [https://www.ebi.ac.uk/pdbsum/3qvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvf ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/Q56187_STRWE Q56187_STRWE]
	We present here the crystal structures of fosfomycin resistance protein (FomA) complexed with MgATP, with ATP and fosfomycin, with MgADP and fosfomycin vanadate, with MgADP and the product of the enzymatic reaction, fosfomycin monophosphate, and with ADP at 1.87, 1.58, 1.85, 1.57, and 1.85 A resolution, respectively. Structures of these complexes that approximate different reaction steps allowed us to distinguish the catalytically active conformation of ATP and to reconstruct the model of the MgATP.fosfomycin complex. According to the model, the triphosphate tail of the nucleotide is aligned toward the phosphonate moiety of fosfomycin, in contast to the previously published MgAMPPNP complex, with the attacking fosfomycin oxygen positioned 4 A from the gamma-phosphorus of ATP. Site-directed mutagenesis studies and comparison of these structures with that of homologous N-acetyl-l-glutamate and isopentenyl phosphate kinases allowed us to propose a model of phosphorylation of fosfomycin by FomA enzyme. A Mg cation ligates all three phosphate groups of ATP and together with positively charged K216, K9, K18, and H58 participates in the dissipation of negative charge during phosphoryl transfer, indicating that the transferred phosphate group is highly negatively charged, which would be expected for an associative mechanism. K216 polarizes the gamma-phosphoryl group of ATP. K9, K18, and H58 participate in stabilization of the transition state. D150 and D208 play organizational roles in catalysis. S148, S149, and T210 participate in fosfomycin binding, with T210 being crucial for catalysis. Hence, it appears that as in the homologous enzymes, FomA-catalyzed phosphoryl transfer takes place by an in-line predominantly associative mechanism.

	Structural and Biochemical Insights into the Mechanism of Fosfomycin Phosphorylation by Fosfomycin Resistance Kinase FomA.,Pakhomova S, Bartlett SG, Doerner PA, Newcomer ME Biochemistry. 2011 Jul 18. PMID:21728358<ref>PMID:~~21728358<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3qvf" style="background-color:#fffaf0;"><~~/~~div>~~
	~~== References ==~~
	~~<references~~/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Actinomyces wedmorensis milard and burr 1926]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Bartlett, S G~~]]		[[Category: Streptomyces wedmorensis]]
	[[Category: ~~Doerner, P A~~]]		[[Category: Bartlett SG]]
	[[Category: ~~Newcomer, M E~~]]		[[Category: Doerner PA]]
	[[Category: ~~Pakhomova, S~~]]		[[Category: Newcomer ME]]
	[[Category: ~~Antibiotic resistance]]~~		[[Category: Pakhomova S]]
	~~[[Category: Fosfomycin]]~~
	~~[[Category: Kinase]]~~
	~~[[Category: Phosphoryl transfer]]~~
	~~[[Category: Transferase~~]]

OCA at 05:38, 15 June 2022

2022-06-15T05:38:01Z

← Older revision		Revision as of 05:38, 15 June 2022
Line 1:		Line 1:

	==Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate==		==Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate==
	<StructureSection load='3qvf' size='340' side='right' caption='[[3qvf]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3qvf' size='340' side='right'caption='[[3qvf]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_wedmorensis"_milard_and_burr_1926 "actinomyces wedmorensis" milard and burr 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_wedmorensis"_milard_and_burr_1926 "actinomyces wedmorensis" milard and burr 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QVF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qun\|3qun]], [[3quo\|3quo]], [[3qur\|3qur]], [[3d40\|3d40]], [[3d41\|3d41]], [[3qvh\|3qvh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qun\|3qun]], [[3quo\|3quo]], [[3qur\|3qur]], [[3d40\|3d40]], [[3d41\|3d41]], [[3qvh\|3qvh]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fomA, fosfomycin resistance kinase ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 "Actinomyces wedmorensis" Milard and Burr 1926])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fomA, fosfomycin resistance kinase ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 "Actinomyces wedmorensis" Milard and Burr 1926])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [~~http~~://pdbe.org/3qvf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qvf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [https://pdbe.org/3qvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [https://www.ebi.ac.uk/pdbsum/3qvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Actinomyces wedmorensis milard and burr 1926]]		[[Category: Actinomyces wedmorensis milard and burr 1926]]
			[[Category: Large Structures]]
	[[Category: Bartlett, S G]]		[[Category: Bartlett, S G]]
	[[Category: Doerner, P A]]		[[Category: Doerner, P A]]

OCA at 18:43, 4 August 2016

2016-08-04T18:43:22Z

← Older revision		Revision as of 18:43, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate==		==Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate==
	<StructureSection load='3qvf' size='340' side='right' caption='[[3qvf]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3qvf' size='340' side='right' caption='[[3qvf]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_wedmorensis Streptomyces~~ wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_wedmorensis"_milard_and_burr_1926 "actinomyces wedmorensis" milard and burr 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qun\|3qun]], [[3quo\|3quo]], [[3qur\|3qur]], [[3d40\|3d40]], [[3d41\|3d41]], [[3qvh\|3qvh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qun\|3qun]], [[3quo\|3quo]], [[3qur\|3qur]], [[3d40\|3d40]], [[3d41\|3d41]], [[3qvh\|3qvh]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fomA, fosfomycin resistance kinase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 ~~Streptomyces~~ wedmorensis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fomA, fosfomycin resistance kinase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 "Actinomyces wedmorensis" Milard and Burr 1926])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [http://www.ebi.ac.uk/pdbsum/3qvf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [http://pdbe.org/3qvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [http://www.ebi.ac.uk/pdbsum/3qvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3qvf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Streptomyces~~ wedmorensis]]		[[Category: Actinomyces wedmorensis milard and burr 1926]]
	[[Category: Bartlett, S G]]		[[Category: Bartlett, S G]]
	[[Category: Doerner, P A]]		[[Category: Doerner, P A]]

OCA at 23:11, 3 January 2015

2015-01-03T23:11:56Z

← Older revision		Revision as of 23:11, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVF FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qun\|3qun]], [[3quo\|3quo]], [[3qur\|3qur]], [[3d40\|3d40]], [[3d41\|3d41]], [[3qvh\|3qvh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qun\|3qun]], [[3quo\|3quo]], [[3qur\|3qur]], [[3d40\|3d40]], [[3d41\|3d41]], [[3qvh\|3qvh]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fomA, fosfomycin resistance kinase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fomA, fosfomycin resistance kinase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [http://www.ebi.ac.uk/pdbsum/3qvf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [http://www.ebi.ac.uk/pdbsum/3qvf PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Structural and Biochemical Insights into the Mechanism of Fosfomycin Phosphorylation by Fosfomycin Resistance Kinase FomA.,Pakhomova S, Bartlett SG, Doerner PA, Newcomer ME Biochemistry. 2011 Jul 18. PMID:21728358<ref>PMID:21728358</ref>		Structural and Biochemical Insights into the Mechanism of Fosfomycin Phosphorylation by Fosfomycin Resistance Kinase FomA.,Pakhomova S, Bartlett SG, Doerner PA, Newcomer ME Biochemistry. 2011 Jul 18. PMID:21728358<ref>PMID:21728358</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces wedmorensis]]		[[Category: Streptomyces wedmorensis]]
	[[Category: Bartlett, S G.]]		[[Category: Bartlett, S G]]
	[[Category: Doerner, P A.]]		[[Category: Doerner, P A]]
	[[Category: Newcomer, M E.]]		[[Category: Newcomer, M E]]
	[[Category: Pakhomova, S.]]		[[Category: Pakhomova, S]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Fosfomycin]]		[[Category: Fosfomycin]]

OCA at 04:46, 5 June 2014

2014-06-05T04:46:54Z

← Older revision		Revision as of 04:46, 5 June 2014
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	[[~~Image~~:~~3qvf~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate==
			<StructureSection load='3qvf' size='340' side='right' caption='[[3qvf]], [[Resolution\|resolution]] 1.85Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3qvf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVF FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FV1:DIHYDROXY{[(2R,3S)-3-METHYLOXIRAN-2-YL]PHOSPHONATO-KAPPAO}OXOVANADIUM'>FV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qun\|3qun]], [[3quo\|3quo]], [[3qur\|3qur]], [[3d40\|3d40]], [[3d41\|3d41]], [[3qvh\|3qvh]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fomA, fosfomycin resistance kinase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qvf RCSB], [http://www.ebi.ac.uk/pdbsum/3qvf PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			We present here the crystal structures of fosfomycin resistance protein (FomA) complexed with MgATP, with ATP and fosfomycin, with MgADP and fosfomycin vanadate, with MgADP and the product of the enzymatic reaction, fosfomycin monophosphate, and with ADP at 1.87, 1.58, 1.85, 1.57, and 1.85 A resolution, respectively. Structures of these complexes that approximate different reaction steps allowed us to distinguish the catalytically active conformation of ATP and to reconstruct the model of the MgATP.fosfomycin complex. According to the model, the triphosphate tail of the nucleotide is aligned toward the phosphonate moiety of fosfomycin, in contast to the previously published MgAMPPNP complex, with the attacking fosfomycin oxygen positioned 4 A from the gamma-phosphorus of ATP. Site-directed mutagenesis studies and comparison of these structures with that of homologous N-acetyl-l-glutamate and isopentenyl phosphate kinases allowed us to propose a model of phosphorylation of fosfomycin by FomA enzyme. A Mg cation ligates all three phosphate groups of ATP and together with positively charged K216, K9, K18, and H58 participates in the dissipation of negative charge during phosphoryl transfer, indicating that the transferred phosphate group is highly negatively charged, which would be expected for an associative mechanism. K216 polarizes the gamma-phosphoryl group of ATP. K9, K18, and H58 participate in stabilization of the transition state. D150 and D208 play organizational roles in catalysis. S148, S149, and T210 participate in fosfomycin binding, with T210 being crucial for catalysis. Hence, it appears that as in the homologous enzymes, FomA-catalyzed phosphoryl transfer takes place by an in-line predominantly associative mechanism.

	~~<!--~~		Structural and Biochemical Insights into the Mechanism of Fosfomycin Phosphorylation by Fosfomycin Resistance Kinase FomA.,Pakhomova S, Bartlett SG, Doerner PA, Newcomer ME Biochemistry. 2011 Jul 18. PMID:21728358<ref>PMID:21728358</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3qvf"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3qvf\| PDB=3qvf \| SCENE= }}~~

	~~===Crystal structure~~ of ~~fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	~~<!--~~		<references/>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21728358}}, adds the Publication Abstract to~~ the ~~page~~		__TOC__
	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21728358 is the PubMed ID number~~.		</StructureSection>
	-->
	~~{{ABSTRACT_PUBMED_21728358}}~~

	~~==About this Structure==~~
	~~[[3qvf]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVF OCA].~~

	==~~Reference~~==
	<~~ref group="xtra">PMID:021728358<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Streptomyces wedmorensis]]		[[Category: Streptomyces wedmorensis]]
	[[Category: Bartlett, S G.]]		[[Category: Bartlett, S G.]]

OCA at 04:59, 27 July 2011

2011-07-27T04:59:10Z

← Older revision		Revision as of 04:59, 27 July 2011
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	[[Image:3qvf.~~jpg~~\|left\|200px]]		[[Image:3qvf.png\|left\|200px]]

	<!--		<!--
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	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Phosphoryl transfer]]		[[Category: Phosphoryl transfer]]
	[[Category: Transferase~~-transferase inhibitor complex~~]]		[[Category: Transferase]]

OCA at 06:44, 20 July 2011

2011-07-20T06:44:53Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3qvf.jpg|left|200px]]
-The entry 3qvf is ON HOLD  until Paper Publication
+<!--
-Authors: Pakhomova, S., Bartlett, S.G., Doerner, P.A., Newcomer, M.E.
+===Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate===
-Description: Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate

OCA at 04:47, 8 June 2011

2011-06-08T04:47:35Z

← Older revision		Revision as of 04:47, 8 June 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3qvf is ON HOLD		The entry 3qvf is ON HOLD until Paper Publication

	Authors: Pakhomova, S., Bartlett, S.G., Doerner, P.A., Newcomer, M.E.		Authors: Pakhomova, S., Bartlett, S.G., Doerner, P.A., Newcomer, M.E.

	Description: Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate		Description: Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate

OCA at 08:04, 16 March 2011

2011-03-16T08:04:18Z

← Older revision		Revision as of 08:04, 16 March 2011
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	The entry 3qvf is ON HOLD		The entry 3qvf is ON HOLD

	Authors: Pakhomova, ~~Svetlana~~ , Bartlett, ~~Sue~~ G., Doerner, ~~Pamela~~ A., Newcomer, ~~Marcia~~ E.		Authors: Pakhomova, S., Bartlett, S.G., Doerner, P.A., Newcomer, M.E.

	Description: Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate		Description: Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgADP and fosfomycin vanadate