3ref - Revision history

OCA at 12:20, 14 March 2024

2024-03-14T12:20:35Z

← Older revision		Revision as of 12:20, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3ref' size='340' side='right'caption='[[3ref]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3ref' size='340' side='right'caption='[[3ref]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Enthi Enthi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3REF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3REF FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GDP:GUANOSINE-5~~-~~DIPHOSPHATE'>GDP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3reg\|3reg]], [[1ftn\|1ftn]]~~</~~div~~><~~/td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~EhRho1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [https://pdbe.org/3ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [https://www.ebi.ac.uk/pdbsum/3ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ref ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [https://pdbe.org/3ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [https://www.ebi.ac.uk/pdbsum/3ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ref ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	=~~= Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/RHO1_ENTHI RHO1_ENTHI]
	The single-celled human parasite Entamoeba histolytica possesses a dynamic actin cytoskeleton vital for its intestinal and systemic pathogenicity. The E. histolytica genome encodes several Rho family GTPases known to regulate cytoskeletal dynamics. EhRho1, the first family member identified, was reported to be insensitive to the Rho GTPase-specific Clostridium botulinum C3 exoenzyme, raising the possibility that it may be a misclassified Ras family member. Here, we report the crystal structures of EhRho1 in both active and inactive states. EhRho1 is activated by a conserved switch mechanism, but diverges from mammalian Rho GTPases in lacking a signature Rho insert helix. EhRho1 engages a homolog of mDia, EhFormin1, suggesting a role in mediating serum-stimulated actin reorganization and microtubule formation during mitosis. EhRho1, but not a constitutively active mutant, interacts with a newly identified EhRhoGDI in a prenylation-dependent manner. Furthermore, constitutively active EhRho1 induces actin stress fiber formation in mammalian fibroblasts, thereby identifying it as a functional Rho family GTPase. EhRho1 exhibits a fast rate of nucleotide exchange relative to mammalian Rho GTPases due to a distinctive switch one isoleucine residue reminiscent of the constitutively active F28L mutation in human Cdc42, which for the latter protein, is sufficient for cellular transformation. Nonconserved, nucleotide-interacting residues within EhRho1, revealed by the crystal structure models, were observed to contribute a moderating influence on fast spontaneous nucleotide exchange. Collectively, these observations indicate that EhRho1 is a bona fide member of the Rho GTPase family, albeit with unique structural and functional aspects compared with mammalian Rho GTPases.

	~~Unique structural and nucleotide exchange features of the Rho1 GTPase of Entamoeba histolytica.,Bosch DE, Wittchen ES, Qiu C, Burridge K, Siderovski DP J Biol Chem. 2011 Nov 11;286(45)~~:~~39236-46. Epub 2011 Sep 19. PMID:21930699<ref>PMID:21930699<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 3ref" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references~~/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Enthi~~]]		[[Category: Entamoeba histolytica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bosch~~, D E~~]]		[[Category: Bosch DE]]
	[[Category: Qiu, C]]		[[Category: Qiu C]]
	[[Category: Siderovski~~, D P]]~~		[[Category: Siderovski DP]]
	~~[[Category: Cytoskeleton]]~~
	~~[[Category: Gtp-binding]]~~
	~~[[Category: Lipoprotein]]~~
	~~[[Category: Nucleotide-binding]]~~
	~~[[Category: Prenylation]]~~
	~~[[Category: Signaling protein~~]]

OCA at 05:59, 15 June 2022

2022-06-15T05:59:59Z

← Older revision		Revision as of 05:59, 15 June 2022
Line 1:		Line 1:

	==Crystal structure of EhRho1 bound to GDP and Magnesium==		==Crystal structure of EhRho1 bound to GDP and Magnesium==
	<StructureSection load='3ref' size='340' side='right' caption='[[3ref]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3ref' size='340' side='right'caption='[[3ref]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3REF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3REF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3reg\|3reg]], [[1ftn\|1ftn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3reg\|3reg]], [[1ftn\|1ftn]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EhRho1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EhRho1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [~~http~~://pdbe.org/3ref PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ref PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ref ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [https://pdbe.org/3ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [https://www.ebi.ac.uk/pdbsum/3ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ref ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Enthi]]		[[Category: Enthi]]
			[[Category: Large Structures]]
	[[Category: Bosch, D E]]		[[Category: Bosch, D E]]
	[[Category: Qiu, C]]		[[Category: Qiu, C]]

OCA at 08:49, 11 August 2016

2016-08-11T08:49:34Z

← Older revision		Revision as of 08:49, 11 August 2016
Line 1:		Line 1:

	==Crystal structure of EhRho1 bound to GDP and Magnesium==		==Crystal structure of EhRho1 bound to GDP and Magnesium==
	<StructureSection load='3ref' size='340' side='right' caption='[[3ref]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3ref' size='340' side='right' caption='[[3ref]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Entamoeba_histolytica Entamoeba histolytica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3reg\|3reg]], [[1ftn\|1ftn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3reg\|3reg]], [[1ftn\|1ftn]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EhRho1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ~~Entamoeba histolytica~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EhRho1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [http://www.ebi.ac.uk/pdbsum/3ref PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [http://pdbe.org/3ref PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [http://www.ebi.ac.uk/pdbsum/3ref PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ref ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3ref" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Entamoeba histolytica~~]]		[[Category: Enthi]]
	[[Category: Bosch, D E]]		[[Category: Bosch, D E]]
	[[Category: Qiu, C]]		[[Category: Qiu, C]]

OCA at 11:00, 4 January 2015

2015-01-04T11:00:18Z

← Older revision		Revision as of 11:00, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REF FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3reg\|3reg]], [[1ftn\|1ftn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3reg\|3reg]], [[1ftn\|1ftn]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EhRho1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 Entamoeba histolytica])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EhRho1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 Entamoeba histolytica])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [http://www.ebi.ac.uk/pdbsum/3ref PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [http://www.ebi.ac.uk/pdbsum/3ref PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Unique structural and nucleotide exchange features of the Rho1 GTPase of Entamoeba histolytica.,Bosch DE, Wittchen ES, Qiu C, Burridge K, Siderovski DP J Biol Chem. 2011 Nov 11;286(45):39236-46. Epub 2011 Sep 19. PMID:21930699<ref>PMID:21930699</ref>		Unique structural and nucleotide exchange features of the Rho1 GTPase of Entamoeba histolytica.,Bosch DE, Wittchen ES, Qiu C, Burridge K, Siderovski DP J Biol Chem. 2011 Nov 11;286(45):39236-46. Epub 2011 Sep 19. PMID:21930699<ref>PMID:21930699</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
	[[Category: Bosch, D E.]]		[[Category: Bosch, D E]]
	[[Category: Qiu, C.]]		[[Category: Qiu, C]]
	[[Category: Siderovski, D P.]]		[[Category: Siderovski, D P]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]

OCA at 04:51, 5 June 2014

2014-06-05T04:51:30Z

← Older revision		Revision as of 04:51, 5 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~3ref~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of EhRho1 bound to GDP and Magnesium==
			<StructureSection load='3ref' size='340' side='right' caption='[[3ref]], [[Resolution\|resolution]] 1.95Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3ref]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REF FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3reg\|3reg]], [[1ftn\|1ftn]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EhRho1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 Entamoeba histolytica])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ref OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ref RCSB], [http://www.ebi.ac.uk/pdbsum/3ref PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The single-celled human parasite Entamoeba histolytica possesses a dynamic actin cytoskeleton vital for its intestinal and systemic pathogenicity. The E. histolytica genome encodes several Rho family GTPases known to regulate cytoskeletal dynamics. EhRho1, the first family member identified, was reported to be insensitive to the Rho GTPase-specific Clostridium botulinum C3 exoenzyme, raising the possibility that it may be a misclassified Ras family member. Here, we report the crystal structures of EhRho1 in both active and inactive states. EhRho1 is activated by a conserved switch mechanism, but diverges from mammalian Rho GTPases in lacking a signature Rho insert helix. EhRho1 engages a homolog of mDia, EhFormin1, suggesting a role in mediating serum-stimulated actin reorganization and microtubule formation during mitosis. EhRho1, but not a constitutively active mutant, interacts with a newly identified EhRhoGDI in a prenylation-dependent manner. Furthermore, constitutively active EhRho1 induces actin stress fiber formation in mammalian fibroblasts, thereby identifying it as a functional Rho family GTPase. EhRho1 exhibits a fast rate of nucleotide exchange relative to mammalian Rho GTPases due to a distinctive switch one isoleucine residue reminiscent of the constitutively active F28L mutation in human Cdc42, which for the latter protein, is sufficient for cellular transformation. Nonconserved, nucleotide-interacting residues within EhRho1, revealed by the crystal structure models, were observed to contribute a moderating influence on fast spontaneous nucleotide exchange. Collectively, these observations indicate that EhRho1 is a bona fide member of the Rho GTPase family, albeit with unique structural and functional aspects compared with mammalian Rho GTPases.

	~~<!--~~		Unique structural and nucleotide exchange features of the Rho1 GTPase of Entamoeba histolytica.,Bosch DE, Wittchen ES, Qiu C, Burridge K, Siderovski DP J Biol Chem. 2011 Nov 11;286(45):39236-46. Epub 2011 Sep 19. PMID:21930699<ref>PMID:21930699</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3ref"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3ref\| PDB=3ref \| SCENE= }}~~

	~~===Crystal structure~~ of ~~EhRho1 bound to GDP and Magnesium===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	~~<!--~~		<references/>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21930699}}, adds the Publication Abstract to~~ the ~~page~~		__TOC__
	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21930699 is the PubMed ID number~~.		</StructureSection>
	-->
	~~{{ABSTRACT_PUBMED_21930699}}~~

	~~==About this Structure==~~
	~~[[3ref]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA].~~

	==~~Reference~~==
	<~~ref group="xtra">PMID:021930699<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
	[[Category: Bosch, D E.]]		[[Category: Bosch, D E.]]

OCA at 05:52, 14 March 2012

2012-03-14T05:52:18Z

← Older revision		Revision as of 05:52, 14 March 2012
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	[[Image:3ref.~~jpg~~\|left\|200px]]		[[Image:3ref.png\|left\|200px]]

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	===Crystal structure of EhRho1 bound to GDP and Magnesium===		===Crystal structure of EhRho1 bound to GDP and Magnesium===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_21930699}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 21930699 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_21930699}}

	==About this Structure==		==About this Structure==
	[[3ref]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA].		[[3ref]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REF OCA].

			==Reference==
			<ref group="xtra">PMID:021930699</ref><references group="xtra"/>
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
	[[Category: Bosch, D E.]]		[[Category: Bosch, D E.]]

OCA at 05:46, 28 September 2011

2011-09-28T05:46:37Z

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-'''Unreleased structure'''
+[[Image:3ref.jpg|left|200px]]
-The entry 3ref is ON HOLD  until Paper Publication
+<!--
-Authors: Bosch, D.E., Qiu, C., Siderovski, D.P.
+===Crystal structure of EhRho1 bound to GDP and Magnesium===
-Description: Crystal structure of EhRho1 bound to GDP and Magnesium

OCA at 06:54, 27 April 2011

2011-04-27T06:54:51Z

← Older revision		Revision as of 06:54, 27 April 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3ref is ON HOLD		The entry 3ref is ON HOLD until Paper Publication

	Authors: Bosch, D.E., Qiu, C., Siderovski, D.P.		Authors: Bosch, D.E., Qiu, C., Siderovski, D.P.

	Description: Crystal structure of EhRho1 bound to GDP and Magnesium		Description: Crystal structure of EhRho1 bound to GDP and Magnesium

OCA at 10:21, 21 April 2011

2011-04-21T10:21:03Z

← Older revision		Revision as of 10:21, 21 April 2011
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	Authors: Bosch, D.E., Qiu, C., Siderovski, D.P.		Authors: Bosch, D.E., Qiu, C., Siderovski, D.P.

	Description: Crystal structure of EhRho1 bound to GDP and ~~magnesium~~		Description: Crystal structure of EhRho1 bound to GDP and Magnesium

OCA: Protected "3ref" [edit=sysop:move=sysop]

2011-04-13T04:59:46Z

Protected "3ref" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:59, 13 April 2011
(No difference)