3rix - Revision history

OCA at 12:25, 14 March 2024

2024-03-14T12:25:31Z

← Older revision		Revision as of 12:25, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rix]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rix]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=923:(5Z)-4-HYDROXY-3-[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]-5-{[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]METHYLIDENE}FURAN-2(5H)-ONE'>923</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=923:(5Z)-4-HYDROXY-3-[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]-5-{[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]METHYLIDENE}FURAN-2(5H)-ONE'>923</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [https://pdbe.org/3rix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [https://www.ebi.ac.uk/pdbsum/3rix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rix ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [https://pdbe.org/3rix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [https://www.ebi.ac.uk/pdbsum/3rix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm.		[https://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY] Produces green light with a wavelength of 562 nm.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The chemical diversity of nature has tremendous potential for the discovery of molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, and macro- and microorganisms has curtailed their use in lead discovery. Here, we describe a process for leveraging the concentration-response curves obtained from quantitative HTS to improve the initial selection of "actives" from a library of partially fractionated natural product extracts derived from marine actinomycetes and fungi. By using pharmacological activity, the first-pass CRC paradigm improves the probability that labor-intensive subsequent steps of reculturing, extraction, and bioassay-guided isolation of active component(s) target the most promising strains and growth conditions. We illustrate how this process identified a family of fungal metabolites as potent inhibitors of firefly luciferase, subsequently resolved in molecular detail by X-ray crystallography.

	Titration-based screening for evaluation of natural product extracts: identification of an aspulvinone family of luciferase inhibitors.,Cruz PG, Auld DS, Schultz PJ, Lovell S, Battaile KP, MacArthur R, Shen M, Tamayo-Castillo G, Inglese J, Sherman DH Chem Biol. 2011 Nov 23;18(11):1442-52. PMID:22118678<ref>PMID:22118678</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3rix" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Luciferase 3D structures\|Luciferase 3D structures]]		*[[Luciferase 3D structures\|Luciferase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Photinus pyralis]]		[[Category: Photinus pyralis]]
	[[Category: Auld~~, D S~~]]		[[Category: Auld DS]]
	[[Category: Battaile~~, K P~~]]		[[Category: Battaile KP]]
	[[Category: Inglese, J]]		[[Category: Inglese J]]
	[[Category: Lopez~~, P C~~]]		[[Category: Lopez PC]]
	[[Category: Lovell, S]]		[[Category: Lovell S]]
	[[Category: MacArthur, R]]		[[Category: MacArthur R]]
	[[Category: Schultz~~, P J~~]]		[[Category: Schultz PJ]]
	[[Category: Shen, M]]		[[Category: Shen M]]
	[[Category: Sherman~~, D H~~]]		[[Category: Sherman DH]]
	[[Category: Tamayo, G]]		[[Category: Tamayo G]]
	~~[[Category: Aspulvinone]]~~
	~~[[Category: Luminescence]]~~
	~~[[Category: Monooxygenase]]~~
	~~[[Category: Natural product extract]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Oxidoreductase-oxidoreductase inhibitor complex]]~~
	~~[[Category: Photoprotein~~]]

OCA at 10:12, 22 June 2022

2022-06-22T10:12:26Z

← Older revision		Revision as of 10:12, 22 June 2022
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	<StructureSection load='3rix' size='340' side='right'caption='[[3rix]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3rix' size='340' side='right'caption='[[3rix]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rix]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RIX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rix]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=923:(5Z)-4-HYDROXY-3-[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]-5-{[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]METHYLIDENE}FURAN-2(5H)-ONE'>923</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=923:(5Z)-4-HYDROXY-3-[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]-5-{[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]METHYLIDENE}FURAN-2(5H)-ONE'>923</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [~~http~~://pdbe.org/3rix PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rix PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rix ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [https://pdbe.org/3rix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [https://www.ebi.ac.uk/pdbsum/3rix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm.		[[https://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
	*[[Luciferase\|Luciferase]]		*[[Luciferase 3D structures\|Luciferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:41, 14 August 2019

2019-08-14T17:41:22Z

← Older revision		Revision as of 17:41, 14 August 2019
Line 1:		Line 1:

	==1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex==		==1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex==
	<StructureSection load='3rix' size='340' side='right' caption='[[3rix]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3rix' size='340' side='right'caption='[[3rix]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rix]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rix]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIX FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3rix" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3rix" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Luciferase\|Luciferase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Photinus pyralis]]		[[Category: Photinus pyralis]]
	[[Category: Auld, D S]]		[[Category: Auld, D S]]

OCA at 09:26, 8 November 2017

2017-11-08T09:26:39Z

← Older revision		Revision as of 09:26, 8 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3rix" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3rix" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Luciferase\|Luciferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:54, 5 August 2016

2016-08-05T16:54:25Z

← Older revision		Revision as of 16:54, 5 August 2016
Line 1:		Line 1:

	==1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex==		==1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex==
	<StructureSection load='3rix' size='340' side='right' caption='[[3rix]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3rix' size='340' side='right' caption='[[3rix]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=923:(5Z)-4-HYDROXY-3-[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]-5-{[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]METHYLIDENE}FURAN-2(5H)-ONE'>923</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=923:(5Z)-4-HYDROXY-3-[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]-5-{[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]METHYLIDENE}FURAN-2(5H)-ONE'>923</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [http://www.ebi.ac.uk/pdbsum/3rix PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [http://pdbe.org/3rix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [http://www.ebi.ac.uk/pdbsum/3rix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3rix" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 04:11, 25 December 2014

2014-12-25T04:11:58Z

← Older revision		Revision as of 04:11, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [http://www.ebi.ac.uk/pdbsum/3rix PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [http://www.ebi.ac.uk/pdbsum/3rix PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:23, 9 December 2014

2014-12-09T12:23:06Z

← Older revision		Revision as of 12:23, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:3rix.~~png\|left\|200px~~]]		==1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex==
			<StructureSection load='3rix' size='340' side='right' caption='[[3rix]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3rix]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIX FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=923:(5Z)-4-HYDROXY-3-[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]-5-{[(2R)-2-(2-HYDROXYPROPAN-2-YL)-2,3-DIHYDRO-1-BENZOFURAN-5-YL]METHYLIDENE}FURAN-2(5H)-ONE'>923</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [http://www.ebi.ac.uk/pdbsum/3rix PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The chemical diversity of nature has tremendous potential for the discovery of molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, and macro- and microorganisms has curtailed their use in lead discovery. Here, we describe a process for leveraging the concentration-response curves obtained from quantitative HTS to improve the initial selection of "actives" from a library of partially fractionated natural product extracts derived from marine actinomycetes and fungi. By using pharmacological activity, the first-pass CRC paradigm improves the probability that labor-intensive subsequent steps of reculturing, extraction, and bioassay-guided isolation of active component(s) target the most promising strains and growth conditions. We illustrate how this process identified a family of fungal metabolites as potent inhibitors of firefly luciferase, subsequently resolved in molecular detail by X-ray crystallography.

	~~{{STRUCTURE_3rix\| PDB=3rix \| SCENE= }}~~		Titration-based screening for evaluation of natural product extracts: identification of an aspulvinone family of luciferase inhibitors.,Cruz PG, Auld DS, Schultz PJ, Lovell S, Battaile KP, MacArthur R, Shen M, Tamayo-Castillo G, Inglese J, Sherman DH Chem Biol. 2011 Nov 23;18(11):1442-52. PMID:22118678<ref>PMID:22118678</ref>

	~~===1~~.~~7A resolution structure~~ of ~~a firefly luciferase-Aspulvinone J inhibitor complex===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22118678}}~~		==See Also==
			*[[Luciferase\|Luciferase]]
	==~~About this Structure~~==		== References ==
	[[~~3rix]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIX OCA~~].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:022118678<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Photinus pyralis]]		[[Category: Photinus pyralis]]
	[[Category: Auld, D S.]]		[[Category: Auld, D S]]
	[[Category: Battaile, K P.]]		[[Category: Battaile, K P]]
	[[Category: Inglese, J.]]		[[Category: Inglese, J]]
	[[Category: Lopez, P C.]]		[[Category: Lopez, P C]]
	[[Category: Lovell, S.]]		[[Category: Lovell, S]]
	[[Category: MacArthur, R.]]		[[Category: MacArthur, R]]
	[[Category: Schultz, P J.]]		[[Category: Schultz, P J]]
	[[Category: Shen, M.]]		[[Category: Shen, M]]
	[[Category: Sherman, D H.]]		[[Category: Sherman, D H]]
	[[Category: Tamayo, G.]]		[[Category: Tamayo, G]]
	[[Category: Aspulvinone]]		[[Category: Aspulvinone]]
	[[Category: Luminescence]]		[[Category: Luminescence]]

OCA at 09:41, 5 December 2012

2012-12-05T09:41:50Z

← Older revision		Revision as of 09:41, 5 December 2012
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	[[Image:3rix.png\|left\|200px]]		[[Image:3rix.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3rix", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3rix\| PDB=3rix \| SCENE= }}		{{STRUCTURE_3rix\| PDB=3rix \| SCENE= }}

	===1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex===		===1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22118678}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 22118678 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_22118678}}		{{ABSTRACT_PUBMED_22118678}}

OCA at 06:23, 28 March 2012

2012-03-28T06:23:13Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3rix.png|left|200px]]
-The entry 3rix is ON HOLD  until Paper Publication
+<!--
-Authors: Lovell, S., Battaile, K.P., Lopez, P.C., Auld, D.S., Schultz, P.J., MacArthur, R., Shen, M., Tamayo, G., Inglese, J., Sherman, D.H.
+===1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex===
-Description: 1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex

OCA at 05:51, 15 June 2011

2011-06-15T05:51:06Z

← Older revision		Revision as of 05:51, 15 June 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3rix is ON HOLD		The entry 3rix is ON HOLD until Paper Publication

	Authors: Lovell, S., Battaile, K.P., Lopez, P.C., Auld, D.S., Schultz, P.J., MacArthur, R., Shen, M., Tamayo, G., Inglese, J., Sherman, D.H.		Authors: Lovell, S., Battaile, K.P., Lopez, P.C., Auld, D.S., Schultz, P.J., MacArthur, R., Shen, M., Tamayo, G., Inglese, J., Sherman, D.H.

	Description: 1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex		Description: 1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex