3rtb - Revision history

OCA at 12:36, 14 March 2024

2024-03-14T12:36:32Z

@@ Line 10: / Line 10: @@
 == Function ==
 [https://www.uniprot.org/uniprot/NNR_THEMA NNR_THEMA] Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).
 __TOC__
 </StructureSection>

OCA at 12:47, 17 January 2024

2024-01-17T12:47:13Z

← Older revision		Revision as of 12:47, 17 January 2024
Line 3:		Line 3:
	<StructureSection load='3rtb' size='340' side='right'caption='[[3rtb]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3rtb' size='340' side='right'caption='[[3rtb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rtb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43589 Atcc 43589~~] and [https://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RTB FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rtb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RTB FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=A3P:ADENOSINE-3-5~~-~~DIPHOSPHATE'>A3P</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>[[2ax3\|2ax3]], [[3rno\|3rno]], [[3ro7\|3ro7]], [[3roe\|3roe]], [[3rog\|3rog]], [[3rox\|3rox]], [[3roz\|3roz]], [[3rrb\|3rrb]], [[3rre\|3rre]], [[3rrf\|3rrf]], [[3rrj\|3rrj]], [[3rs8\|3rs8]], [[3rs9\|3rs9]], [[3rsf\|3rsf]], [[3rsg\|3rsg]], [[3rsq\|3rsq]], [[3rss\|3rss]], [[3rt7\|3rt7]], [[3rt9\|3rt9]], [[3rta\|3rta]], [[3rtc\|3rtc]], [[3rtd\|3rtd]], [[3rte\|3rte]], [[3rtg\|3rtg]], [[3ru2\|3ru2]], [[3ru3\|3ru3]]</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~tm0922~~, ~~TM_0922 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])~~<~~/td></tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[https://en.wikipedia.org/wiki/ATP-dependent_NAD(P)H-hydrate_dehydratase ATP-dependent NAD(P)H-hydrate dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.93 4.2.1.93] </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [https://pdbe.org/3rtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [https://www.ebi.ac.uk/pdbsum/3rtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rtb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [https://pdbe.org/3rtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [https://www.ebi.ac.uk/pdbsum/3rtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rtb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/NNR_THEMA NNR_THEMA]] Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).		[https://www.uniprot.org/uniprot/NNR_THEMA NNR_THEMA] Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 43589]]~~
	~~[[Category: Escherichia coli]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Cymborowski, M~~]]		[[Category: Thermotoga maritima]]
	[[Category: ~~Lesley, S A~~]]		[[Category: Cymborowski M]]
	[[Category: ~~Minor, W~~]]		[[Category: Lesley SA]]
	[[Category: ~~Shumilin, I A~~]]		[[Category: Minor W]]
	[[Category: ~~Lyase]]~~		[[Category: Shumilin IA]]
	~~[[Category: Unknown function~~]]

OCA at 09:58, 13 April 2022

2022-04-13T09:58:52Z

← Older revision		Revision as of 09:58, 13 April 2022
Line 1:		Line 1:

	==Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate==		==Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate==
	<StructureSection load='3rtb' size='340' side='right' caption='[[3rtb]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3rtb' size='340' side='right'caption='[[3rtb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rtb]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589] and [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RTB FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rtb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RTB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ax3\|2ax3]], [[3rno\|3rno]], [[3ro7\|3ro7]], [[3roe\|3roe]], [[3rog\|3rog]], [[3rox\|3rox]], [[3roz\|3roz]], [[3rrb\|3rrb]], [[3rre\|3rre]], [[3rrf\|3rrf]], [[3rrj\|3rrj]], [[3rs8\|3rs8]], [[3rs9\|3rs9]], [[3rsf\|3rsf]], [[3rsg\|3rsg]], [[3rsq\|3rsq]], [[3rss\|3rss]], [[3rt7\|3rt7]], [[3rt9\|3rt9]], [[3rta\|3rta]], [[3rtc\|3rtc]], [[3rtd\|3rtd]], [[3rte\|3rte]], [[3rtg\|3rtg]], [[3ru2\|3ru2]], [[3ru3\|3ru3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ax3\|2ax3]], [[3rno\|3rno]], [[3ro7\|3ro7]], [[3roe\|3roe]], [[3rog\|3rog]], [[3rox\|3rox]], [[3roz\|3roz]], [[3rrb\|3rrb]], [[3rre\|3rre]], [[3rrf\|3rrf]], [[3rrj\|3rrj]], [[3rs8\|3rs8]], [[3rs9\|3rs9]], [[3rsf\|3rsf]], [[3rsg\|3rsg]], [[3rsq\|3rsq]], [[3rss\|3rss]], [[3rt7\|3rt7]], [[3rt9\|3rt9]], [[3rta\|3rta]], [[3rtc\|3rtc]], [[3rtd\|3rtd]], [[3rte\|3rte]], [[3rtg\|3rtg]], [[3ru2\|3ru2]], [[3ru3\|3ru3]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tm0922, TM_0922 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tm0922, TM_0922 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/ATP-dependent_NAD(P)H-hydrate_dehydratase ATP-dependent NAD(P)H-hydrate dehydratase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.93 4.2.1.93] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/ATP-dependent_NAD(P)H-hydrate_dehydratase ATP-dependent NAD(P)H-hydrate dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.93 4.2.1.93] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [~~http~~://pdbe.org/3rtb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rtb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rtb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [https://pdbe.org/3rtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [https://www.ebi.ac.uk/pdbsum/3rtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rtb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/NNR_THEMA NNR_THEMA]] Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).		[[https://www.uniprot.org/uniprot/NNR_THEMA NNR_THEMA]] Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	[[Category: Atcc 43589]]		[[Category: Atcc 43589]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
			[[Category: Large Structures]]
	[[Category: Cymborowski, M]]		[[Category: Cymborowski, M]]
	[[Category: Lesley, S A]]		[[Category: Lesley, S A]]

OCA at 01:44, 5 August 2016

2016-08-05T01:44:23Z

← Older revision		Revision as of 01:44, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate==		==Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate==
	<StructureSection load='3rtb' size='340' side='right' caption='[[3rtb]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3rtb' size='340' side='right' caption='[[3rtb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rtb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~] and [http://en.wikipedia.org/wiki/~~Thermotoga_maritima Thermotoga maritima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RTB FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rtb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RTB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ax3\|2ax3]], [[3rno\|3rno]], [[3ro7\|3ro7]], [[3roe\|3roe]], [[3rog\|3rog]], [[3rox\|3rox]], [[3roz\|3roz]], [[3rrb\|3rrb]], [[3rre\|3rre]], [[3rrf\|3rrf]], [[3rrj\|3rrj]], [[3rs8\|3rs8]], [[3rs9\|3rs9]], [[3rsf\|3rsf]], [[3rsg\|3rsg]], [[3rsq\|3rsq]], [[3rss\|3rss]], [[3rt7\|3rt7]], [[3rt9\|3rt9]], [[3rta\|3rta]], [[3rtc\|3rtc]], [[3rtd\|3rtd]], [[3rte\|3rte]], [[3rtg\|3rtg]], [[3ru2\|3ru2]], [[3ru3\|3ru3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ax3\|2ax3]], [[3rno\|3rno]], [[3ro7\|3ro7]], [[3roe\|3roe]], [[3rog\|3rog]], [[3rox\|3rox]], [[3roz\|3roz]], [[3rrb\|3rrb]], [[3rre\|3rre]], [[3rrf\|3rrf]], [[3rrj\|3rrj]], [[3rs8\|3rs8]], [[3rs9\|3rs9]], [[3rsf\|3rsf]], [[3rsg\|3rsg]], [[3rsq\|3rsq]], [[3rss\|3rss]], [[3rt7\|3rt7]], [[3rt9\|3rt9]], [[3rta\|3rta]], [[3rtc\|3rtc]], [[3rtd\|3rtd]], [[3rte\|3rte]], [[3rtg\|3rtg]], [[3ru2\|3ru2]], [[3ru3\|3ru3]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tm0922, TM_0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ~~Thermotoga maritima~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tm0922, TM_0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ATP-dependent_NAD(P)H-hydrate_dehydratase ATP-dependent NAD(P)H-hydrate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.93 4.2.1.93] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ATP-dependent_NAD(P)H-hydrate_dehydratase ATP-dependent NAD(P)H-hydrate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.93 4.2.1.93] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [http://www.ebi.ac.uk/pdbsum/3rtb PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [http://pdbe.org/3rtb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [http://www.ebi.ac.uk/pdbsum/3rtb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rtb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/~~Q9X024_THEMA Q9X024_THEMA~~]] Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).		[[http://www.uniprot.org/uniprot/NNR_THEMA NNR_THEMA]] Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3rtb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 43589]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	~~[[Category: Thermotoga maritima]]~~
	[[Category: Cymborowski, M]]		[[Category: Cymborowski, M]]
	[[Category: Lesley, S A]]		[[Category: Lesley, S A]]

OCA at 21:42, 24 December 2014

2014-12-24T21:42:21Z

← Older revision		Revision as of 21:42, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [http://www.ebi.ac.uk/pdbsum/3rtb PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [http://www.ebi.ac.uk/pdbsum/3rtb PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q9X024_THEMA Q9X024_THEMA]] Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:57, 9 December 2014

2014-12-09T12:57:53Z

← Older revision		Revision as of 12:57, 9 December 2014
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	[[~~Image~~:~~3rtb~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate==
			<StructureSection load='3rtb' size='340' side='right' caption='[[3rtb]], [[Resolution\|resolution]] 2.10Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3rtb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RTB FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ax3\|2ax3]], [[3rno\|3rno]], [[3ro7\|3ro7]], [[3roe\|3roe]], [[3rog\|3rog]], [[3rox\|3rox]], [[3roz\|3roz]], [[3rrb\|3rrb]], [[3rre\|3rre]], [[3rrf\|3rrf]], [[3rrj\|3rrj]], [[3rs8\|3rs8]], [[3rs9\|3rs9]], [[3rsf\|3rsf]], [[3rsg\|3rsg]], [[3rsq\|3rsq]], [[3rss\|3rss]], [[3rt7\|3rt7]], [[3rt9\|3rt9]], [[3rta\|3rta]], [[3rtc\|3rtc]], [[3rtd\|3rtd]], [[3rte\|3rte]], [[3rtg\|3rtg]], [[3ru2\|3ru2]], [[3ru3\|3ru3]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tm0922, TM_0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ATP-dependent_NAD(P)H-hydrate_dehydratase ATP-dependent NAD(P)H-hydrate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.93 4.2.1.93] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rtb RCSB], [http://www.ebi.ac.uk/pdbsum/3rtb PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Proteins of unknown function comprise a significant fraction of sequenced genomes. Defining the roles of these proteins is vital to understanding cellular processes. Here, we describe a method to determine a protein function based on the identification of its natural ligand(s) by the crystallographic screening of the binding of a metabolite library, followed by a focused search in the metabolic space. The method was applied to two protein families with unknown function, PF01256 and YjeF_N. The PF01256 proteins, represented by YxkO from Bacillus subtilis and the C-terminal domain of Tm0922 from Thermotoga maritima, were shown to catalyze ADP/ATP-dependent NAD(P)H-hydrate dehydratation, a previously described orphan activity. The YjeF_N proteins, represented by mouse apolipoprotein A-I binding protein and the N-terminal domain of Tm0922, were found to interact with an adenosine diphosphoribose-related substrate and likely serve as ADP-ribosyltransferases. Crystallographic screening of metabolites serves as an efficient tool in functional analyses of uncharacterized proteins.

	~~{{STRUCTURE_3rtb\| PDB=3rtb \| SCENE= }}~~		Identification of Unknown Protein Function Using Metabolite Cocktail Screening.,Shumilin IA, Cymborowski M, Chertihin O, Jha KN, Herr JC, Lesley SA, Joachimiak A, Minor W Structure. 2012 Aug 28. PMID:22940582<ref>PMID:22940582</ref>

	~~===Crystal structure of tm0922~~, a ~~fusion~~ of ~~a domain~~ of ~~unknown function and ADP~~/~~ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22940582}}~~		== References ==
			<references/>
	==~~About this Structure~~==		__TOC__
	~~[[3rtb]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA].		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Cymborowski, M.]]		[[Category: Cymborowski, M]]
	[[Category: Lesley, S A.]]		[[Category: Lesley, S A]]
	[[Category: Minor, W.]]		[[Category: Minor, W]]
	[[Category: Shumilin, I A.]]		[[Category: Shumilin, I A]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 06:26, 19 September 2012

2012-09-19T06:26:23Z

← Older revision		Revision as of 06:26, 19 September 2012
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	~~'''Unreleased structure'''~~		[[Image:3rtb.png\|left\|200px]]

	~~The entry~~ 3rtb ~~is ON HOLD~~		{{STRUCTURE_3rtb\| PDB=3rtb \| SCENE= }}

	~~Authors: Shumilin~~, ~~I.A., Cymborowski, M., Lesley, S.A., Minor, W.~~		===Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate===

	~~Description~~: ~~Crystal structure of tm0922, a fusion of a domain of unknown function~~ and ~~ADP~~/~~ATP~~-~~dependent NAD(P)H-hydrate dehydratase from~~ Thermotoga maritima ~~soaked with Adenosine-3'-5'-Diphosphate~~		{{ABSTRACT_PUBMED_22940582}}

			==About this Structure==
			[[3rtb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTB OCA].
			[[Category: Escherichia coli]]
			[[Category: Thermotoga maritima]]
			[[Category: Cymborowski, M.]]
			[[Category: Lesley, S A.]]
			[[Category: Minor, W.]]
			[[Category: Shumilin, I A.]]
			[[Category: Lyase]]
			[[Category: Unknown function]]

OCA at 05:23, 1 June 2011

2011-06-01T05:23:24Z

← Older revision		Revision as of 05:23, 1 June 2011
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	The entry 3rtb is ON HOLD		The entry 3rtb is ON HOLD

	Authors: Shumilin I.A., Cymborowski, M., Lesley, S.A., Minor, W.		Authors: Shumilin, I.A., Cymborowski, M., Lesley, S.A., Minor, W.

	Description: Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate		Description: Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate

OCA: Protected "3rtb" [edit=sysop:move=sysop]

2011-05-12T04:24:04Z

Protected "3rtb" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:24, 12 May 2011
(No difference)

OCA: New page: '''Unreleased structure''' The entry 3rtb is ON HOLD Authors: Shumilin I.A., Cymborowski, M., Lesley, S.A., Minor, W. Description: Crystal structure of tm0922, a fusion of a domain of ...

2011-05-12T04:24:03Z

New page: '''Unreleased structure''' The entry 3rtb is ON HOLD Authors: Shumilin I.A., Cymborowski, M., Lesley, S.A., Minor, W. Description: Crystal structure of tm0922, a fusion of a domain of ...

New page

'''Unreleased structure'''

The entry 3rtb is ON HOLD

Authors: Shumilin I.A., Cymborowski, M., Lesley, S.A., Minor, W.

Description: Crystal structure of tm0922, a fusion of a domain of unknown function and ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Thermotoga maritima soaked with Adenosine-3'-5'-Diphosphate