3set - Revision history

OCA at 09:50, 1 March 2024

2024-03-01T09:50:07Z

← Older revision		Revision as of 09:50, 1 March 2024
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	<StructureSection load='3set' size='340' side='right'caption='[[3set]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3set' size='340' side='right'caption='[[3set]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3set]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SET FirstGlance]. <br>		<table><tr><td colspan='2'>[[3set]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SET FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLC:ALPHA~~-~~D-GLUCOSE'>GLC</scene>~~, ~~<scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3ser\|3ser]], [[3ses\|3ses]], [[3seu\|3seu]], [[3sev\|3sev]], [[3sew\|3sew]], [[3sex\|3sex]], [[3sey\|3sey]]~~</~~div~~><~~/td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">b4034, JW3994, malE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [https://pdbe.org/3set PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [https://www.ebi.ac.uk/pdbsum/3set PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3set ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [https://pdbe.org/3set PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [https://www.ebi.ac.uk/pdbsum/3set PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3set ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.		[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Combining the concepts of synthetic symmetrization with the approach of engineering metal binding sites, we have developed a new crystallization methodology termed metal-mediated synthetic symmetrization. In this method, pairs of histidine or cysteine mutations are introduced on the surface of target proteins, generating crystal lattice contacts or oligomeric assemblies upon coordination with metal. Metal-mediated synthetic symmetrization greatly expands the packing and oligomeric assembly possibilities of target proteins, thereby increasing the chances of growing diffraction-quality crystals. To demonstrate this method, we designed various T4 lysozyme (T4L) and maltose-binding protein (MBP) mutants and co-crystallized them with one of three metal ions: copper (Cu(2+) ), nickel (Ni(2+) ) or zinc (Zn(2+) ). The approach resulted in 16 new crystal structures - 8 for T4L and 8 for MBP - displaying a variety of oligomeric assemblies and packing modes, representing in total 13 new and distinct crystal forms for these proteins. We discuss the potential utility of the method for crystallizing target proteins of unknown structure by engineering in pairs of histidine or cysteine residues. As an alternate strategy, we propose that the varied crystallization-prone forms of T4L or MBP engineered in this work could be used as crystallization chaperones, by fusing them genetically to target proteins of interest.

	An approach to crystallizing proteins by metal-mediated synthetic symmetrization.,Laganowsky A, Zhao M, Soriaga AB, Sawaya MR, Cascio D, Yeates TO Protein Sci. 2011 Sep 6. doi: 10.1002/pro.727. PMID:21898649<ref>PMID:21898649</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3set" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Maltose-binding protein 3D structures\|Maltose-binding protein 3D structures]]		*[[Maltose-binding protein 3D structures\|Maltose-binding protein 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Ecoli~~]]		[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cascio, D]]		[[Category: Cascio D]]
	[[Category: Laganowsky, A]]		[[Category: Laganowsky A]]
	[[Category: Sawaya~~, M R~~]]		[[Category: Sawaya MR]]
	[[Category: Soriaga~~, A B~~]]		[[Category: Soriaga AB]]
	[[Category: Yeates~~, T O~~]]		[[Category: Yeates TO]]
	[[Category: Zhao, M]]		[[Category: Zhao M]]
	~~[[Category: Metal-mediated synthetic symmetrization]]~~
	~~[[Category: Sugar binding protein]]~~
	~~[[Category: Synthetic symmetrization~~]]

OCA at 07:53, 29 June 2022

2022-06-29T07:53:39Z

← Older revision		Revision as of 07:53, 29 June 2022
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	==Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)==		==Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)==
	<StructureSection load='3set' size='340' side='right' caption='[[3set]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3set' size='340' side='right'caption='[[3set]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3set]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SET FirstGlance]. <br>		<table><tr><td colspan='2'>[[3set]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SET FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MAL~~:~~MALTOSE~~'>~~MAL~~</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ser\|3ser]], [[3ses\|3ses]], [[3seu\|3seu]], [[3sev\|3sev]], [[3sew\|3sew]], [[3sex\|3sex]], [[3sey\|3sey]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ser\|3ser]], [[3ses\|3ses]], [[3seu\|3seu]], [[3sev\|3sev]], [[3sew\|3sew]], [[3sex\|3sex]], [[3sey\|3sey]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4034, JW3994, malE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4034, JW3994, malE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [~~http~~://pdbe.org/3set PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3set PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3set ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [https://pdbe.org/3set PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [https://www.ebi.ac.uk/pdbsum/3set PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3set ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.		[[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
	*[[Maltose-binding protein\|Maltose-binding protein]]		*[[Maltose-binding protein 3D structures\|Maltose-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
			[[Category: Large Structures]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]
	[[Category: Laganowsky, A]]		[[Category: Laganowsky, A]]

OCA at 09:16, 4 August 2016

2016-08-04T09:16:05Z

← Older revision		Revision as of 09:16, 4 August 2016
Line 1:		Line 1:

	==Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)==		==Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)==
	<StructureSection load='3set' size='340' side='right' caption='[[3set]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3set' size='340' side='right' caption='[[3set]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3set]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SET FirstGlance]. <br>		<table><tr><td colspan='2'>[[3set]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SET FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ser\|3ser]], [[3ses\|3ses]], [[3seu\|3seu]], [[3sev\|3sev]], [[3sew\|3sew]], [[3sex\|3sex]], [[3sey\|3sey]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ser\|3ser]], [[3ses\|3ses]], [[3seu\|3seu]], [[3sev\|3sev]], [[3sew\|3sew]], [[3sex\|3sex]], [[3sey\|3sey]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4034, JW3994, malE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4034, JW3994, malE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [http://www.ebi.ac.uk/pdbsum/3set PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [http://pdbe.org/3set PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [http://www.ebi.ac.uk/pdbsum/3set PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3set ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3set" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Escherichia coli~~]]		[[Category: Ecoli]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]
	[[Category: Laganowsky, A]]		[[Category: Laganowsky, A]]

OCA at 08:07, 24 December 2014

2014-12-24T08:07:21Z

← Older revision		Revision as of 08:07, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [http://www.ebi.ac.uk/pdbsum/3set PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [http://www.ebi.ac.uk/pdbsum/3set PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 13:22, 9 December 2014

2014-12-09T13:22:30Z

← Older revision		Revision as of 13:22, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~3set~~.~~png~~\|~~left~~\|~~200px~~]]		==Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)==
			<StructureSection load='3set' size='340' side='right' caption='[[3set]], [[Resolution\|resolution]] 1.90Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3set]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SET FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ser\|3ser]], [[3ses\|3ses]], [[3seu\|3seu]], [[3sev\|3sev]], [[3sew\|3sew]], [[3sex\|3sex]], [[3sey\|3sey]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4034, JW3994, malE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3set FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3set OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3set RCSB], [http://www.ebi.ac.uk/pdbsum/3set PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Combining the concepts of synthetic symmetrization with the approach of engineering metal binding sites, we have developed a new crystallization methodology termed metal-mediated synthetic symmetrization. In this method, pairs of histidine or cysteine mutations are introduced on the surface of target proteins, generating crystal lattice contacts or oligomeric assemblies upon coordination with metal. Metal-mediated synthetic symmetrization greatly expands the packing and oligomeric assembly possibilities of target proteins, thereby increasing the chances of growing diffraction-quality crystals. To demonstrate this method, we designed various T4 lysozyme (T4L) and maltose-binding protein (MBP) mutants and co-crystallized them with one of three metal ions: copper (Cu(2+) ), nickel (Ni(2+) ) or zinc (Zn(2+) ). The approach resulted in 16 new crystal structures - 8 for T4L and 8 for MBP - displaying a variety of oligomeric assemblies and packing modes, representing in total 13 new and distinct crystal forms for these proteins. We discuss the potential utility of the method for crystallizing target proteins of unknown structure by engineering in pairs of histidine or cysteine residues. As an alternate strategy, we propose that the varied crystallization-prone forms of T4L or MBP engineered in this work could be used as crystallization chaperones, by fusing them genetically to target proteins of interest.

	~~{{STRUCTURE_3set\| PDB=3set \| SCENE= }}~~		An approach to crystallizing proteins by metal-mediated synthetic symmetrization.,Laganowsky A, Zhao M, Soriaga AB, Sawaya MR, Cascio D, Yeates TO Protein Sci. 2011 Sep 6. doi: 10.1002/pro.727. PMID:21898649<ref>PMID:21898649</ref>

	~~===Ni-mediated Dimer of Maltose-binding Protein A216H~~/~~K220H by Synthetic Symmetrization (Form I)===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_21898649}}~~

	~~==About this Structure==~~
	~~[[3set]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=3SET OCA].~~

	==See Also==		==See Also==
	*[[Maltose-binding protein\|Maltose-binding protein]]		*[[Maltose-binding protein\|Maltose-binding protein]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:021898649<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Cascio, D.]]		[[Category: Cascio, D]]
	[[Category: Laganowsky, A.]]		[[Category: Laganowsky, A]]
	[[Category: Sawaya, M R.]]		[[Category: Sawaya, M R]]
	[[Category: Soriaga, A B.]]		[[Category: Soriaga, A B]]
	[[Category: Yeates, T O.]]		[[Category: Yeates, T O]]
	[[Category: Zhao, M.]]		[[Category: Zhao, M]]
	[[Category: Metal-mediated synthetic symmetrization]]		[[Category: Metal-mediated synthetic symmetrization]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]
	[[Category: Synthetic symmetrization]]		[[Category: Synthetic symmetrization]]

OCA at 05:41, 20 October 2012

2012-10-20T05:41:04Z

← Older revision		Revision as of 05:41, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3set]] is a 2 chain structure ~~of [[Maltose-binding protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA].		[[3set]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA].

	==See Also==		==See Also==

OCA at 10:49, 25 July 2012

2012-07-25T10:49:13Z

← Older revision		Revision as of 10:49, 25 July 2012
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	[[Image:3set.png\|left\|200px]]		[[Image:3set.png\|left\|200px]]

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	{{STRUCTURE_3set\| PDB=3set \| SCENE= }}		{{STRUCTURE_3set\| PDB=3set \| SCENE= }}

	===Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)===		===Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)===


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	{{ABSTRACT_PUBMED_21898649}}		{{ABSTRACT_PUBMED_21898649}}

	==About this Structure==		==About this Structure==
	[[3set]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA].		[[3set]] is a 2 chain structure of [[Maltose-binding protein]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SET OCA].

			==See Also==
			*[[Maltose-binding protein\|Maltose-binding protein]]

	==Reference==		==Reference==

OCA at 07:37, 2 November 2011

2011-11-02T07:37:50Z

← Older revision		Revision as of 07:37, 2 November 2011
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	[[Image:3set.~~jpg~~\|left\|200px]]		[[Image:3set.png\|left\|200px]]

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OCA at 05:59, 21 September 2011

2011-09-21T05:59:29Z

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-'''Unreleased structure'''
+[[Image:3set.jpg|left|200px]]
-The entry 3set is ON HOLD  until Paper Publication
+<!--
-Authors: Zhao, M., Soriaga, A.B., Laganowsky, A., Sawaya, M.R., Cascio, D., Yeates, T.O.
+===Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)===
-Description: Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)

OCA at 05:43, 29 June 2011

2011-06-29T05:43:50Z

← Older revision		Revision as of 05:43, 29 June 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3set is ON HOLD		The entry 3set is ON HOLD until Paper Publication

	Authors: Zhao, ~~Minglei~~, Soriaga, ~~Angela~~ B., Laganowsky, ~~Arthur~~, Sawaya, ~~Michael~~ R., Cascio, ~~Duilio~~, Yeates, ~~Todd~~ O.		Authors: Zhao, M., Soriaga, A.B., Laganowsky, A., Sawaya, M.R., Cascio, D., Yeates, T.O.

	Description: Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)		Description: Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)