3ss3 - Revision history

OCA at 13:06, 14 March 2024

2024-03-14T13:06:13Z

← Older revision		Revision as of 13:06, 14 March 2024
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	<StructureSection load='3ss3' size='340' side='right'caption='[[3ss3]], [[Resolution\|resolution]] 2.42Å' scene=''>		<StructureSection load='3ss3' size='340' side='right'caption='[[3ss3]], [[Resolution\|resolution]] 2.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.42Å</td></tr>
	~~<tr id='related'><td class="sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[3ss4\|3ss4]]~~, [[~~3ss5~~\|~~3ss5~~]]~~</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Gls, GLS1, mKIAA0838 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10090 LK3 transgenic mice])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [https://pdbe.org/3ss3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [https://www.ebi.ac.uk/pdbsum/3ss3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ss3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [https://pdbe.org/3ss3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [https://www.ebi.ac.uk/pdbsum/3ss3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ss3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/GLSK_MOUSE GLSK_MOUSE]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.<ref>PMID:16641247</ref> <ref>PMID:22373647</ref> <ref>PMID:22228304</ref>		[https://www.uniprot.org/uniprot/GLSK_MOUSE GLSK_MOUSE] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.<ref>PMID:16641247</ref> <ref>PMID:22373647</ref> <ref>PMID:22228304</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Glutamine is an essential nutrient for cancer cell proliferation, especially in the context of citric acid cycle anaplerosis. In this manuscript we present results that collectively demonstrate that, of the three major mammalian glutaminases identified to date, the lesser studied splice variant of the gene gls, known as Glutaminase C (GAC), is important for tumor metabolism. We show that, although levels of both the kidney-type isoforms are elevated in tumor vs. normal tissues, GAC is distinctly mitochondrial. GAC is also most responsive to the activator inorganic phosphate, the content of which is supposedly higher in mitochondria subject to hypoxia. Analysis of X-ray crystal structures of GAC in different bound states suggests a mechanism that introduces the tetramerization-induced lifting of a "gating loop" as essential for the phosphate-dependent activation process. Surprisingly, phosphate binds inside the catalytic pocket rather than at the oligomerization interface. Phosphate also mediates substrate entry by competing with glutamate. A greater tendency to oligomerize differentiates GAC from its alternatively spliced isoform and the cycling of phosphate in and out of the active site distinguishes it from the liver-type isozyme, which is known to be less dependent on this ion.

	Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.,Cassago A, Ferreira AP, Ferreira IM, Fornezari C, Gomes ER, Greene KS, Pereira HM, Garratt RC, Dias SM, Ambrosio AL Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1092-7. Epub 2012 Jan 6. PMID:22228304<ref>PMID:22228304</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3ss3" style="background-color:#fffaf0;"></div~~>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Glutaminase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Ambrosio~~, A L.B~~]]		[[Category: Ambrosio ALB]]
	[[Category: Cerione~~, R A~~]]		[[Category: Cerione RA]]
	[[Category: Dias~~, S M.G]]~~		[[Category: Dias SMG]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: L-glutamine]]~~
	~~[[Category: Mitochondria~~]]

OCA at 08:08, 29 June 2022

2022-06-29T08:08:17Z

← Older revision		Revision as of 08:08, 29 June 2022
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	==Crystal structure of mouse Glutaminase C, ligand-free form==		==Crystal structure of mouse Glutaminase C, ligand-free form==
	<StructureSection load='3ss3' size='340' side='right' caption='[[3ss3]], [[Resolution\|resolution]] 2.42Å' scene=''>		<StructureSection load='3ss3' size='340' side='right'caption='[[3ss3]], [[Resolution\|resolution]] 2.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ss4\|3ss4]], [[3ss5\|3ss5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ss4\|3ss4]], [[3ss5\|3ss5]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gls, GLS1, mKIAA0838 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gls, GLS1, mKIAA0838 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [~~http~~://pdbe.org/3ss3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ss3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ss3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [https://pdbe.org/3ss3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [https://www.ebi.ac.uk/pdbsum/3ss3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ss3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/GLSK_MOUSE GLSK_MOUSE]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.<ref>PMID:16641247</ref> <ref>PMID:22373647</ref> <ref>PMID:22228304</ref>		[[https://www.uniprot.org/uniprot/GLSK_MOUSE GLSK_MOUSE]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.<ref>PMID:16641247</ref> <ref>PMID:22373647</ref> <ref>PMID:22228304</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Glutaminase\|Glutaminase]]		*[[Glutaminase 3D structures\|Glutaminase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Ambrosio, A L.B]]		[[Category: Ambrosio, A L.B]]

OCA at 02:24, 5 August 2016

2016-08-05T02:24:41Z

← Older revision		Revision as of 02:24, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of mouse Glutaminase C, ligand-free form==		==Crystal structure of mouse Glutaminase C, ligand-free form==
	<StructureSection load='3ss3' size='340' side='right' caption='[[3ss3]], [[Resolution\|resolution]] 2.42Å' scene=''>		<StructureSection load='3ss3' size='340' side='right' caption='[[3ss3]], [[Resolution\|resolution]] 2.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ss4\|3ss4]], [[3ss5\|3ss5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ss4\|3ss4]], [[3ss5\|3ss5]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gls, GLS1, mKIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gls, GLS1, mKIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ss3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [http://pdbe.org/3ss3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ss3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ss3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/~~Q69ZX9_MOUSE Q69ZX9_MOUSE~~]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.<ref>PMID:16641247</ref> <ref>PMID:22373647</ref> <ref>PMID:22228304</ref>		[[http://www.uniprot.org/uniprot/GLSK_MOUSE GLSK_MOUSE]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.<ref>PMID:16641247</ref> <ref>PMID:22373647</ref> <ref>PMID:22228304</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3ss3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Ambrosio, A L.B]]		[[Category: Ambrosio, A L.B]]
	[[Category: Cerione, R A]]		[[Category: Cerione, R A]]

OCA at 15:05, 25 December 2014

2014-12-25T15:05:18Z

← Older revision		Revision as of 15:05, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ss4\|3ss4]], [[3ss5\|3ss5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ss4\|3ss4]], [[3ss5\|3ss5]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gls, GLS1, mKIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gls, GLS1, mKIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ss3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ss3 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q69ZX9_MOUSE Q69ZX9_MOUSE]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.<ref>PMID:16641247</ref> <ref>PMID:22373647</ref> <ref>PMID:22228304</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.,Cassago A, Ferreira AP, Ferreira IM, Fornezari C, Gomes ER, Greene KS, Pereira HM, Garratt RC, Dias SM, Ambrosio AL Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1092-7. Epub 2012 Jan 6. PMID:22228304<ref>PMID:22228304</ref>		Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.,Cassago A, Ferreira AP, Ferreira IM, Fornezari C, Gomes ER, Greene KS, Pereira HM, Garratt RC, Dias SM, Ambrosio AL Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1092-7. Epub 2012 Jan 6. PMID:22228304<ref>PMID:22228304</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

Line 26:		Line 28:
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Ambrosio, A L.B.]]		[[Category: Ambrosio, A L.B]]
	[[Category: Cerione, R A.]]		[[Category: Cerione, R A]]
	[[Category: Dias, S M.G~~.]]~~		[[Category: Dias, S M.G]]
	~~[[Category: Glutaminase~~]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: L-glutamine]]		[[Category: L-glutamine]]
	[[Category: Mitochondria]]		[[Category: Mitochondria]]

OCA at 05:29, 5 June 2014

2014-06-05T05:29:04Z

← Older revision		Revision as of 05:29, 5 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~3ss3~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of mouse Glutaminase C, ligand-free form==
			<StructureSection load='3ss3' size='340' side='right' caption='[[3ss3]], [[Resolution\|resolution]] 2.42Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3ss3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SS3 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ss4\|3ss4]], [[3ss5\|3ss5]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gls, GLS1, mKIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ss3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ss3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ss3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ss3 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Glutamine is an essential nutrient for cancer cell proliferation, especially in the context of citric acid cycle anaplerosis. In this manuscript we present results that collectively demonstrate that, of the three major mammalian glutaminases identified to date, the lesser studied splice variant of the gene gls, known as Glutaminase C (GAC), is important for tumor metabolism. We show that, although levels of both the kidney-type isoforms are elevated in tumor vs. normal tissues, GAC is distinctly mitochondrial. GAC is also most responsive to the activator inorganic phosphate, the content of which is supposedly higher in mitochondria subject to hypoxia. Analysis of X-ray crystal structures of GAC in different bound states suggests a mechanism that introduces the tetramerization-induced lifting of a "gating loop" as essential for the phosphate-dependent activation process. Surprisingly, phosphate binds inside the catalytic pocket rather than at the oligomerization interface. Phosphate also mediates substrate entry by competing with glutamate. A greater tendency to oligomerize differentiates GAC from its alternatively spliced isoform and the cycling of phosphate in and out of the active site distinguishes it from the liver-type isozyme, which is known to be less dependent on this ion.

	<!--		Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.,Cassago A, Ferreira AP, Ferreira IM, Fornezari C, Gomes ER, Greene KS, Pereira HM, Garratt RC, Dias SM, Ambrosio AL Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1092-7. Epub 2012 Jan 6. PMID:22228304<ref>PMID:22228304</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3ss3"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3ss3\| PDB=3ss3 \| SCENE= }}~~

	~~===Crystal structure~~ of ~~mouse Glutaminase C, ligand-free form===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>

			==See Also==
	~~<!--~~		*[[Glutaminase\|Glutaminase]]
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22228304}}, adds the Publication Abstract to the page~~		== References ==
	~~(as it appears on PubMed at http://www.pubmed.gov), where 22228304 is the PubMed ID number.~~		<references/>
	~~-->~~		__TOC__
	~~{{ABSTRACT_PUBMED_22228304}}~~		</StructureSection>

	==~~About this Structure~~==
	[[~~3ss3]~~] ~~is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA].~~

	==~~Reference~~==
	<~~ref group="xtra">PMID:022228304<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 05:20, 4 April 2012

2012-04-04T05:20:05Z

← Older revision		Revision as of 05:20, 4 April 2012
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	===Crystal structure of mouse Glutaminase C, ligand-free form===		===Crystal structure of mouse Glutaminase C, ligand-free form===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22228304}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22228304 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22228304}}

	==About this Structure==		==About this Structure==
	[[3ss3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA].		[[3ss3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SS3 OCA].

			==Reference==
			<ref group="xtra">PMID:022228304</ref><references group="xtra"/>
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 06:27, 11 January 2012

2012-01-11T06:27:29Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3ss3.png|left|200px]]
-The entry 3ss3 is ON HOLD  until Paper Publication
+<!--
-Authors: Ambrosio, A.L.B., Dias, S.M.G., Cerione, R.A.
+===Crystal structure of mouse Glutaminase C, ligand-free form===
-Description: Crystal structure of mouse Glutaminase C, ligand-free form

OCA at 05:30, 10 August 2011

2011-08-10T05:30:50Z

← Older revision		Revision as of 05:30, 10 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3ss3 is ON HOLD		The entry 3ss3 is ON HOLD until Paper Publication

	Authors: Ambrosio, A.L.B., Dias, S.M.G., Cerione, R.A.		Authors: Ambrosio, A.L.B., Dias, S.M.G., Cerione, R.A.

	Description: Crystal structure of mouse Glutaminase C, ligand-free form		Description: Crystal structure of mouse Glutaminase C, ligand-free form

OCA: Protected "3ss3" [edit=sysop:move=sysop]

2011-07-13T06:56:29Z

Protected "3ss3" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:56, 13 July 2011
(No difference)

OCA: New page: '''Unreleased structure''' The entry 3ss3 is ON HOLD Authors: Ambrosio, A.L.B., Dias, S.M.G., Cerione, R.A. Description: Crystal structure of mouse Glutaminase C, ligand-free form

2011-07-13T06:56:28Z

New page: '''Unreleased structure''' The entry 3ss3 is ON HOLD Authors: Ambrosio, A.L.B., Dias, S.M.G., Cerione, R.A. Description: Crystal structure of mouse Glutaminase C, ligand-free form

New page

'''Unreleased structure'''

The entry 3ss3 is ON HOLD

Authors: Ambrosio, A.L.B., Dias, S.M.G., Cerione, R.A.

Description: Crystal structure of mouse Glutaminase C, ligand-free form