3syn - Revision history

OCA at 10:17, 13 August 2026

2026-08-13T10:17:23Z

← Older revision		Revision as of 10:17, 13 August 2026
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.		[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF-effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete.

			Structural basis for the molecular evolution of SRP-GTPase activation by protein.,Bange G, Kummerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I Nat Struct Mol Biol. 2011 Nov 6;18(12):1376-80. doi: 10.1038/nsmb.2141. PMID:22056770<ref>PMID:22056770</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3syn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 15:		Line 24:
	*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]		*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]
	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]		*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:12, 14 March 2024

2024-03-14T13:12:13Z

← Older revision		Revision as of 13:12, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3syn' size='340' side='right'caption='[[3syn]], [[Resolution\|resolution]] 3.06Å' scene=''>		<StructureSection load='3syn' size='340' side='right'caption='[[3syn]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SYN FirstGlance]. <br>		<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SYN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.063Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2px0\|2px0]], [[2px3\|2px3]]</div></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [https://pdbe.org/3syn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [https://www.ebi.ac.uk/pdbsum/3syn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [https://pdbe.org/3syn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [https://www.ebi.ac.uk/pdbsum/3syn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU]] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. ~~[[https://www.uniprot.org/uniprot/YLXH_BACSU YLXH_BACSU]] Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF.<ref>PMID:22056770</ref>~~		[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF-effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete.

	Structural basis for the molecular evolution of SRP-GTPase activation by protein.,Bange G, Kummerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I Nat Struct Mol Biol. 2011 Nov 6;18(12):1376-80. doi: 10.1038/nsmb.2141. PMID:22056770<ref>PMID:22056770</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3syn" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
Line 25:		Line 15:
	*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]		*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]
	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]		*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]		[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bange, G]]		[[Category: Bange G]]
	[[Category: Kuemmerer, N]]		[[Category: Kuemmerer N]]
	[[Category: Sinning, I]]		[[Category: Sinning I]]
	[[Category: Wild, K]]		[[Category: Wild K]]
	~~[[Category: Biosynthetic protein]]~~
	~~[[Category: Flagellum]]~~
	~~[[Category: Gtp-binding protein]]~~
	~~[[Category: Gtpase activating protein]]~~
	~~[[Category: Protein transport]]~~
	~~[[Category: Srp gtpase]]~~
	~~[[Category: Type 3 secretion system~~]]

OCA at 08:16, 29 June 2022

2022-06-29T08:16:10Z

← Older revision		Revision as of 08:16, 29 June 2022
Line 1:		Line 1:

	==Crystal structure of FlhF in complex with its activator==		==Crystal structure of FlhF in complex with its activator==
	<StructureSection load='3syn' size='340' side='right' caption='[[3syn]], [[Resolution\|resolution]] 3.06Å' scene=''>		<StructureSection load='3syn' size='340' side='right'caption='[[3syn]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SYN FirstGlance]. <br>		<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SYN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2px0\|2px0]], [[2px3\|2px3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2px0\|2px0]], [[2px3\|2px3]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [~~http~~://pdbe.org/3syn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3syn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [https://pdbe.org/3syn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [https://www.ebi.ac.uk/pdbsum/3syn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU]] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. [[~~http~~://www.uniprot.org/uniprot/YLXH_BACSU YLXH_BACSU]] Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF.<ref>PMID:22056770</ref>		[[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU]] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. [[https://www.uniprot.org/uniprot/YLXH_BACSU YLXH_BACSU]] Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF.<ref>PMID:22056770</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
	*[[GTP-binding protein\|GTP-binding protein]]		*[[Flagellar biosynthetic protein\|Flagellar biosynthetic protein]]
			*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]
			*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus globigii migula 1900~~]]		[[Category: Vibrio subtilis ehrenberg 1835]]
			[[Category: Large Structures]]
	[[Category: Bange, G]]		[[Category: Bange, G]]
	[[Category: Kuemmerer, N]]		[[Category: Kuemmerer, N]]

OCA at 10:41, 5 August 2016

2016-08-05T10:41:09Z

← Older revision		Revision as of 10:41, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of FlhF in complex with its activator==		==Crystal structure of FlhF in complex with its activator==
	<StructureSection load='3syn' size='340' side='right' caption='[[3syn]], [[Resolution\|resolution]] 3.06Å' scene=''>		<StructureSection load='3syn' size='340' side='right' caption='[[3syn]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SYN FirstGlance]. <br>		<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SYN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2px0\|2px0]], [[2px3\|2px3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2px0\|2px0]], [[2px3\|2px3]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [http://www.ebi.ac.uk/pdbsum/3syn PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [http://pdbe.org/3syn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [http://www.ebi.ac.uk/pdbsum/3syn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3syn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bacillus ~~subtilis~~]]		[[Category: Bacillus globigii migula 1900]]
	[[Category: Bange, G]]		[[Category: Bange, G]]
	[[Category: Kuemmerer, N]]		[[Category: Kuemmerer, N]]

OCA at 18:06, 25 December 2014

2014-12-25T18:06:34Z

← Older revision		Revision as of 18:06, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [http://www.ebi.ac.uk/pdbsum/3syn PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [http://www.ebi.ac.uk/pdbsum/3syn PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU]] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. [[http://www.uniprot.org/uniprot/YLXH_BACSU YLXH_BACSU]] Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF.<ref>PMID:22056770</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:50, 9 December 2014

2014-12-09T10:50:21Z

← Older revision		Revision as of 10:50, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~3syn~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of FlhF in complex with its activator==
			<StructureSection load='3syn' size='340' side='right' caption='[[3syn]], [[Resolution\|resolution]] 3.06Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SYN FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2px0\|2px0]], [[2px3\|2px3]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [http://www.ebi.ac.uk/pdbsum/3syn PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF-effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete.

	~~{{STRUCTURE_3syn\| PDB=3syn \| SCENE= }}~~		Structural basis for the molecular evolution of SRP-GTPase activation by protein.,Bange G, Kummerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I Nat Struct Mol Biol. 2011 Nov 6;18(12):1376-80. doi: 10.1038/nsmb.2141. PMID:22056770<ref>PMID:22056770</ref>

	~~===Crystal structure~~ of ~~FlhF in complex with its activator===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22056770}}~~

	~~==About this Structure==~~
	~~[[3syn]] is a 8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis Bacillus subtilis]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=3SYN OCA].~~

	==See Also==		==See Also==
	*[[GTP-binding protein\|GTP-binding protein]]		*[[GTP-binding protein\|GTP-binding protein]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:022056770<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Bange, G.]]		[[Category: Bange, G]]
	[[Category: Kuemmerer, N.]]		[[Category: Kuemmerer, N]]
	[[Category: Sinning, I.]]		[[Category: Sinning, I]]
	[[Category: Wild, K.]]		[[Category: Wild, K]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]
	[[Category: Flagellum]]		[[Category: Flagellum]]

OCA at 19:47, 20 October 2012

2012-10-20T19:47:15Z

← Older revision		Revision as of 19:47, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3syn]] is a 8 chain structure ~~of [[GTP-binding protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA].		[[3syn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA].

	==See Also==		==See Also==

OCA at 08:28, 27 July 2012

2012-07-27T08:28:09Z

← Older revision		Revision as of 08:28, 27 July 2012
Line 1:		Line 1:
	[[Image:3syn.png\|left\|200px]]		[[Image:3syn.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3syn", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3syn\| PDB=3syn \| SCENE= }}		{{STRUCTURE_3syn\| PDB=3syn \| SCENE= }}

	===Crystal structure of FlhF in complex with its activator===		===Crystal structure of FlhF in complex with its activator===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22056770}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 22056770 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_22056770}}		{{ABSTRACT_PUBMED_22056770}}

OCA at 05:09, 23 May 2012

2012-05-23T05:09:34Z

← Older revision		Revision as of 05:09, 23 May 2012
Line 1:		Line 1:
	[[Image:3syn.~~jpg~~\|left\|200px]]		[[Image:3syn.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of FlhF in complex with its activator===		===Crystal structure of FlhF in complex with its activator===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22056770}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22056770 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22056770}}

	==About this Structure==		==About this Structure==
	[[3syn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA].		[[3syn]] is a 8 chain structure of [[GTP-binding protein]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA].

			==See Also==
			*[[GTP-binding protein\|GTP-binding protein]]

			==Reference==
			<ref group="xtra">PMID:022056770</ref><references group="xtra"/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Bange, G.]]		[[Category: Bange, G.]]

OCA at 11:50, 9 November 2011

2011-11-09T11:50:17Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3syn.jpg|left|200px]]
-The entry 3syn is ON HOLD  until Paper Publication
+<!--
-Authors: Bange, G., Kuemmerer, N., Wild, K., Sinning, I.
+===Crystal structure of FlhF in complex with its activator===
-Description: Crystal structure of FlhF in complex with its activator