3tip - Revision history

OCA at 10:09, 1 March 2024

2024-03-01T10:09:25Z

← Older revision		Revision as of 10:09, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3tip' size='340' side='right'caption='[[3tip]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3tip' size='340' side='right'caption='[[3tip]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Staa8 Staa8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TIP FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3tiq\|3tiq]]</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7009Å</td></tr>
	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">SAOUHSC_02798, sasG ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax~~.~~cgi?mode=Info~~&~~srchmode=5&id=93061 STAA8])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [https://pdbe.org/3tip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [https://www.ebi.ac.uk/pdbsum/3tip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tip ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [https://pdbe.org/3tip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [https://www.ebi.ac.uk/pdbsum/3tip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tip ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/SASG_STAA8 SASG_STAA8]] Promotes adhesion of bacterial cells to human squamous nasal epithelial cells, a phenomenon which is likely to be important in nasal colonization. Forms short, extremely dense and thin fibrils all over the bacterial surface. Does not bind to either buccal cells or non-differentiated keratinocytes. Promotes cellular aggregation leading to biofilm formation.<ref>PMID:14523109</ref> <ref>PMID:17660408</ref>		[https://www.uniprot.org/uniprot/SASG_STAA8 SASG_STAA8] Promotes adhesion of bacterial cells to human squamous nasal epithelial cells, a phenomenon which is likely to be important in nasal colonization. Forms short, extremely dense and thin fibrils all over the bacterial surface. Does not bind to either buccal cells or non-differentiated keratinocytes. Promotes cellular aggregation leading to biofilm formation.<ref>PMID:14523109</ref> <ref>PMID:17660408</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Staphylococcus aureus and Staphylococcus epidermidis form communities (called biofilms) on inserted medical devices, leading to infections that affect many millions of patients worldwide and cause substantial morbidity and mortality. As biofilms are resistant to antibiotics, device removal is often required to resolve the infection. Thus, there is a need for new therapeutic strategies and molecular data that might assist their development. Surface proteins S. aureus surface protein G (SasG) and accumulation-associated protein (S. epidermidis) promote biofilm formation through their "B" regions. B regions contain tandemly arrayed G5 domains interspersed with approximately 50 residue sequences (herein called E) and have been proposed to mediate intercellular accumulation through Zn(2+)-mediated homodimerization. Although E regions are predicted to be unstructured, SasG and accumulation-associated protein form extended fibrils on the bacterial surface. Here we report structures of E-G5 and G5-E-G5 from SasG and biophysical characteristics of single and multidomain fragments. E sequences fold cooperatively and form interlocking interfaces with G5 domains in a head-to-tail fashion, resulting in a contiguous, elongated, monomeric structure. E and G5 domains lack a compact hydrophobic core, and yet G5 domain and multidomain constructs have thermodynamic stabilities only slightly lower than globular proteins of similar size. Zn(2+) does not cause SasG domains to form dimers. The work reveals a paradigm for formation of fibrils on the 100-nm scale and suggests that biofilm accumulation occurs through a mechanism distinct from the "zinc zipper." Finally, formation of two domains by each repeat (as in SasG) might reduce misfolding in proteins when the tandem arrangement of highly similar sequences is advantageous.

	Staphylococcal biofilm-forming protein has a contiguous rod-like structure.,Gruszka DT, Wojdyla JA, Bingham RJ, Turkenburg JP, Manfield IW, Steward A, Leech AP, Geoghegan JA, Foster TJ, Clarke J, Potts JR Proc Natl Acad Sci U S A. 2012 Apr 9. PMID:22493247<ref>PMID:22493247</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3tip" style="background-color:#fffaf0;"></div~~>
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Staa8~~]]		[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
	[[Category: Gruszka~~, D T~~]]		[[Category: Gruszka DT]]
	[[Category: Potts~~, J R~~]]		[[Category: Potts JR]]
	[[Category: Turkenburg~~, J P~~]]		[[Category: Turkenburg JP]]
	[[Category: Wojdyla~~, J A]]~~		[[Category: Wojdyla JA]]
	~~[[Category: Biofilm formation]]~~
	~~[[Category: Single-layer beta sheet]]~~
	~~[[Category: Structural protein]]~~
	~~[[Category: Surface~~]]

OCA at 16:59, 6 July 2022

2022-07-06T16:59:47Z

← Older revision		Revision as of 16:59, 6 July 2022
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	==Crystal structure of Staphylococcus aureus SasG E-G52 module==		==Crystal structure of Staphylococcus aureus SasG E-G52 module==
	<StructureSection load='3tip' size='340' side='right' caption='[[3tip]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3tip' size='340' side='right'caption='[[3tip]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TIP FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tiq\|3tiq]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tiq\|3tiq]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_02798, sasG ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_02798, sasG ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [~~http~~://pdbe.org/3tip PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tip PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tip ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [https://pdbe.org/3tip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [https://www.ebi.ac.uk/pdbsum/3tip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tip ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/SASG_STAA8 SASG_STAA8]] Promotes adhesion of bacterial cells to human squamous nasal epithelial cells, a phenomenon which is likely to be important in nasal colonization. Forms short, extremely dense and thin fibrils all over the bacterial surface. Does not bind to either buccal cells or non-differentiated keratinocytes. Promotes cellular aggregation leading to biofilm formation.<ref>PMID:14523109</ref> <ref>PMID:17660408</ref>		[[https://www.uniprot.org/uniprot/SASG_STAA8 SASG_STAA8]] Promotes adhesion of bacterial cells to human squamous nasal epithelial cells, a phenomenon which is likely to be important in nasal colonization. Forms short, extremely dense and thin fibrils all over the bacterial surface. Does not bind to either buccal cells or non-differentiated keratinocytes. Promotes cellular aggregation leading to biofilm formation.<ref>PMID:14523109</ref> <ref>PMID:17660408</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Staa8]]		[[Category: Staa8]]
	[[Category: Gruszka, D T]]		[[Category: Gruszka, D T]]

OCA at 15:27, 4 August 2016

2016-08-04T15:27:30Z

← Older revision		Revision as of 15:27, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of Staphylococcus aureus SasG E-G52 module==		==Crystal structure of Staphylococcus aureus SasG E-G52 module==
	<StructureSection load='3tip' size='340' side='right' caption='[[3tip]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3tip' size='340' side='right' caption='[[3tip]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIP FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tiq\|3tiq]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tiq\|3tiq]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_02798, sasG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~46170 Staphylococcus aureus subsp. aureus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_02798, sasG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [http://www.ebi.ac.uk/pdbsum/3tip PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [http://pdbe.org/3tip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [http://www.ebi.ac.uk/pdbsum/3tip PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tip ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3tip" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Staphylococcus aureus subsp. aureus~~]]		[[Category: Staa8]]
	[[Category: Gruszka, D T]]		[[Category: Gruszka, D T]]
	[[Category: Potts, J R]]		[[Category: Potts, J R]]

OCA at 16:24, 24 December 2014

2014-12-24T16:24:48Z

← Older revision		Revision as of 16:24, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIP FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tiq\|3tiq]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tiq\|3tiq]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_02798, sasG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_02798, sasG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [http://www.ebi.ac.uk/pdbsum/3tip PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [http://www.ebi.ac.uk/pdbsum/3tip PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/SASG_STAA8 SASG_STAA8]] Promotes adhesion of bacterial cells to human squamous nasal epithelial cells, a phenomenon which is likely to be important in nasal colonization. Forms short, extremely dense and thin fibrils all over the bacterial surface. Does not bind to either buccal cells or non-differentiated keratinocytes. Promotes cellular aggregation leading to biofilm formation.<ref>PMID:14523109</ref> <ref>PMID:17660408</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	Staphylococcal biofilm-forming protein has a contiguous rod-like structure.,Gruszka DT, Wojdyla JA, Bingham RJ, Turkenburg JP, Manfield IW, Steward A, Leech AP, Geoghegan JA, Foster TJ, Clarke J, Potts JR Proc Natl Acad Sci U S A. 2012 Apr 9. PMID:22493247<ref>PMID:22493247</ref>		Staphylococcal biofilm-forming protein has a contiguous rod-like structure.,Gruszka DT, Wojdyla JA, Bingham RJ, Turkenburg JP, Manfield IW, Steward A, Leech AP, Geoghegan JA, Foster TJ, Clarke J, Potts JR Proc Natl Acad Sci U S A. 2012 Apr 9. PMID:22493247<ref>PMID:22493247</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Gruszka, D T.]]		[[Category: Gruszka, D T]]
	[[Category: Potts, J R.]]		[[Category: Potts, J R]]
	[[Category: Turkenburg, J P.]]		[[Category: Turkenburg, J P]]
	[[Category: Wojdyla, J A.]]		[[Category: Wojdyla, J A]]
	[[Category: Biofilm formation]]		[[Category: Biofilm formation]]
	[[Category: Single-layer beta sheet]]		[[Category: Single-layer beta sheet]]
	[[Category: Structural protein]]		[[Category: Structural protein]]
	[[Category: Surface]]		[[Category: Surface]]

OCA at 06:40, 5 June 2014

2014-06-05T06:40:44Z

← Older revision		Revision as of 06:40, 5 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~3tip~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of Staphylococcus aureus SasG E-G52 module==
			<StructureSection load='3tip' size='340' side='right' caption='[[3tip]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3tip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIP FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tiq\|3tiq]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_02798, sasG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tip RCSB], [http://www.ebi.ac.uk/pdbsum/3tip PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Staphylococcus aureus and Staphylococcus epidermidis form communities (called biofilms) on inserted medical devices, leading to infections that affect many millions of patients worldwide and cause substantial morbidity and mortality. As biofilms are resistant to antibiotics, device removal is often required to resolve the infection. Thus, there is a need for new therapeutic strategies and molecular data that might assist their development. Surface proteins S. aureus surface protein G (SasG) and accumulation-associated protein (S. epidermidis) promote biofilm formation through their "B" regions. B regions contain tandemly arrayed G5 domains interspersed with approximately 50 residue sequences (herein called E) and have been proposed to mediate intercellular accumulation through Zn(2+)-mediated homodimerization. Although E regions are predicted to be unstructured, SasG and accumulation-associated protein form extended fibrils on the bacterial surface. Here we report structures of E-G5 and G5-E-G5 from SasG and biophysical characteristics of single and multidomain fragments. E sequences fold cooperatively and form interlocking interfaces with G5 domains in a head-to-tail fashion, resulting in a contiguous, elongated, monomeric structure. E and G5 domains lack a compact hydrophobic core, and yet G5 domain and multidomain constructs have thermodynamic stabilities only slightly lower than globular proteins of similar size. Zn(2+) does not cause SasG domains to form dimers. The work reveals a paradigm for formation of fibrils on the 100-nm scale and suggests that biofilm accumulation occurs through a mechanism distinct from the "zinc zipper." Finally, formation of two domains by each repeat (as in SasG) might reduce misfolding in proteins when the tandem arrangement of highly similar sequences is advantageous.

	<!--		Staphylococcal biofilm-forming protein has a contiguous rod-like structure.,Gruszka DT, Wojdyla JA, Bingham RJ, Turkenburg JP, Manfield IW, Steward A, Leech AP, Geoghegan JA, Foster TJ, Clarke J, Potts JR Proc Natl Acad Sci U S A. 2012 Apr 9. PMID:22493247<ref>PMID:22493247</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3tip"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3tip\| PDB=3tip \| SCENE= }}~~

	~~===Crystal structure~~ of ~~Staphylococcus aureus SasG E-G52 module===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	~~<!--~~		<references/>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22493247}}, adds~~ the ~~Publication Abstract to the page~~		__TOC__
	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22493247 is the PubMed ID number~~.		</StructureSection>
	-->
	~~{{ABSTRACT_PUBMED_22493247}}~~

	~~==About this Structure==~~
	~~[[3tip]] is a 1 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA].

	==~~Reference~~==
	<~~ref group="xtra">PMID:022493247<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Gruszka, D T.]]		[[Category: Gruszka, D T.]]

OCA at 08:14, 25 April 2012

2012-04-25T08:14:54Z

← Older revision		Revision as of 08:14, 25 April 2012
Line 1:		Line 1:
	[[Image:3tip.~~jpg~~\|left\|200px]]		[[Image:3tip.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of Staphylococcus aureus SasG E-G52 module===		===Crystal structure of Staphylococcus aureus SasG E-G52 module===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22493247}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22493247 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22493247}}

	==About this Structure==		==About this Structure==
	[[3tip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA].		[[3tip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIP OCA].

			==Reference==
			<ref group="xtra">PMID:022493247</ref><references group="xtra"/>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Gruszka, D T.]]		[[Category: Gruszka, D T.]]

OCA at 07:56, 18 April 2012

2012-04-18T07:56:41Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3tip.jpg|left|200px]]
-The entry 3tip is ON HOLD  until Paper Publication
+<!--
-Authors: Gruszka, D.T., Wojdyla, J.A., Turkenburg, J.P., Potts, J.R.
+===Crystal structure of Staphylococcus aureus SasG E-G52 module===
-Description: Crystal structure of Staphylococcus aureus SasG E-G52 module

OCA at 05:48, 7 September 2011

2011-09-07T05:48:03Z

← Older revision		Revision as of 05:48, 7 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3tip is ON HOLD		The entry 3tip is ON HOLD until Paper Publication

	Authors: Gruszka, D.T., Wojdyla, J.A., Turkenburg, J.P., Potts, J.R.		Authors: Gruszka, D.T., Wojdyla, J.A., Turkenburg, J.P., Potts, J.R.

	Description: Crystal structure of Staphylococcus aureus SasG E-G52 module		Description: Crystal structure of Staphylococcus aureus SasG E-G52 module

OCA: Protected "3tip" [edit=sysop:move=sysop]

2011-08-31T06:25:55Z

Protected "3tip" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:25, 31 August 2011
(No difference)

OCA: New page: '''Unreleased structure''' The entry 3tip is ON HOLD Authors: Gruszka, D.T., Wojdyla, J.A., Turkenburg, J.P., Potts, J.R. Description: Crystal structure of Staphylococcus aureus SasG E...

2011-08-31T06:25:54Z

New page: '''Unreleased structure''' The entry 3tip is ON HOLD Authors: Gruszka, D.T., Wojdyla, J.A., Turkenburg, J.P., Potts, J.R. Description: Crystal structure of Staphylococcus aureus SasG E...

New page

'''Unreleased structure'''

The entry 3tip is ON HOLD

Authors: Gruszka, D.T., Wojdyla, J.A., Turkenburg, J.P., Potts, J.R.

Description: Crystal structure of Staphylococcus aureus SasG E-G52 module