3tk2 - Revision history

OCA at 13:31, 14 March 2024

2024-03-14T13:31:30Z

← Older revision		Revision as of 13:31, 14 March 2024
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	<StructureSection load='3tk2' size='340' side='right'caption='[[3tk2]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='3tk2' size='340' side='right'caption='[[3tk2]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tk2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"chromobacterium_janthinum"_(zopf)_ford_1927 "chromobacterium janthinum" (zopf) ford 1927~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TK2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tk2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chromobacterium_violaceum Chromobacterium violaceum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TK2 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>~~, ~~<scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.35Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em~~;~~'>[[3tcy\|3tcy]], [[3tk4\|3tk4]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>phhA, CV_3180 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~536~~ "~~Chromobacterium janthinum~~" ~~(Zopf) Ford 1927])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk2 OCA], [https://pdbe.org/3tk2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tk2 RCSB], [https://www.ebi.ac.uk/pdbsum/3tk2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk2 OCA], [https://pdbe.org/3tk2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tk2 RCSB], [https://www.ebi.ac.uk/pdbsum/3tk2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk2 ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/PH4H_CHRVO PH4H_CHRVO]
	Phenylalanine hydroxylase (PAH) is a non-heme iron enzyme that catalyzes oxidation of phenylalanine to tyrosine, a reaction that must be kept under tight regulatory control. Mammalian PAH has a regulatory domain in which binding of the substrate leads to allosteric activation of the enzyme. However, the existence of PAH regulation in evolutionarily distant organisms, for example some bacteria in which it occurs, has so far been underappreciated. In an attempt to crystallographically characterize substrate binding by PAH from Chromobacterium violaceum, a single-domain monomeric enzyme, electron density for phenylalanine was observed at a distal site 15.7 A from the active site. Isothermal titration calorimetry (ITC) experiments revealed a dissociation constant of 24 +/- 1.1 muM for phenylalanine. Under the same conditions, ITC revealed no detectable binding for alanine, tyrosine, or isoleucine, indicating the distal site may be selective for phenylalanine. Point mutations of amino acid residues in the distal site that contact phenylalanine (F258A, Y155A, T254A) led to impaired binding, consistent with the presence of distal site binding in solution. Although kinetic analysis revealed that the distal site mutants suffer discernible loss of their catalytic activity, X-ray crystallographic analysis of Y155A and F258A, the two mutants with the most noticeable decrease in activity, revealed no discernible change in the structure of their active sites, suggesting that the effect of distal binding may result from protein dynamics in solution.

	~~An additional substrate binding site in a bacterial phenylalanine hydroxylase.,Ronau JA, Paul LN, Fuchs JE, Corn IR, Wagner KT, Liedl KR, Abu-Omar MM, Das C Eur Biophys J. 2013 Jul 17. PMID~~:~~23860686<ref>PMID:23860686<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 3tk2" style="background-color:#fffaf0;"><~~/~~div>~~

	==See Also==		==See Also==
	*[[Hydroxylases 3D structures\|Hydroxylases 3D structures]]		*[[Hydroxylases 3D structures\|Hydroxylases 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Chromobacterium violaceum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Phenylalanine 4-monooxygenase]]~~		[[Category: Abu-Omar MM]]
	[[Category: Abu-Omar~~, M M~~]]		[[Category: Das C]]
	[[Category: Das, C]]		[[Category: Ronau JA]]
	[[Category: Ronau~~, J A]]~~
	~~[[Category: Beta]]~~
	~~[[Category: Hydroxylase]]~~
	~~[[Category: Hydroxylation]]~~
	~~[[Category: Mixed alpha]]~~
	~~[[Category: Molecular oxygen]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Phenylalanine]]~~
	~~[[Category: Protein-substate complex]]~~
	~~[[Category: Tetrahydrobiopterin~~]]

OCA at 17:01, 6 July 2022

2022-07-06T17:01:39Z

← Older revision		Revision as of 17:01, 6 July 2022
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	<StructureSection load='3tk2' size='340' side='right'caption='[[3tk2]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='3tk2' size='340' side='right'caption='[[3tk2]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tk2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"chromobacterium_janthinum"_(zopf)_ford_1927 "chromobacterium janthinum" (zopf) ford 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TK2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tk2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"chromobacterium_janthinum"_(zopf)_ford_1927 "chromobacterium janthinum" (zopf) ford 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TK2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tcy\|3tcy]], [[3tk4\|3tk4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tcy\|3tcy]], [[3tk4\|3tk4]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phhA, CV_3180 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536 "Chromobacterium janthinum" (Zopf) Ford 1927])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phhA, CV_3180 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536 "Chromobacterium janthinum" (Zopf) Ford 1927])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk2 OCA], [~~http~~://pdbe.org/3tk2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tk2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tk2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk2 OCA], [https://pdbe.org/3tk2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tk2 RCSB], [https://www.ebi.ac.uk/pdbsum/3tk2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk2 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:53, 20 November 2019

2019-11-20T15:53:45Z

← Older revision		Revision as of 15:53, 20 November 2019
Line 1:		Line 1:

	==Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site==		==Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site==
	<StructureSection load='3tk2' size='340' side='right' caption='[[3tk2]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='3tk2' size='340' side='right'caption='[[3tk2]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chromobacterium_violaceum Chromobacterium violaceum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TK2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"chromobacterium_janthinum"_(zopf)_ford_1927 "chromobacterium janthinum" (zopf) ford 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TK2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tcy\|3tcy]], [[3tk4\|3tk4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tcy\|3tcy]], [[3tk4\|3tk4]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phhA, CV_3180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536 Chromobacterium ~~violaceum~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phhA, CV_3180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536 "Chromobacterium janthinum" (Zopf) Ford 1927])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tk2 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk2 OCA], [http://pdbe.org/3tk2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tk2 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk2 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3tk2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[~~Phenylalanine hydroxylase~~\|~~Phenylalanine hydroxylase~~]]		*[[Hydroxylases 3D structures\|Hydroxylases 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Chromobacterium violaceum~~]]		[[Category: Large Structures]]
	[[Category: Phenylalanine 4-monooxygenase]]		[[Category: Phenylalanine 4-monooxygenase]]
	[[Category: Abu-Omar, M M]]		[[Category: Abu-Omar, M M]]

OCA at 09:59, 4 January 2015

2015-01-04T09:59:50Z

← Older revision		Revision as of 09:59, 4 January 2015
Line 1:		Line 1:
	~~{{STRUCTURE_3tk2\| PDB=3tk2 \| SCENE= }}~~		==Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site==
	===Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site===		<StructureSection load='3tk2' size='340' side='right' caption='[[3tk2]], [[Resolution\|resolution]] 1.35Å' scene=''>
	~~{{ABSTRACT_PUBMED_23860686}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3tk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chromobacterium_violaceum Chromobacterium violaceum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TK2 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tcy\|3tcy]], [[3tk4\|3tk4]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phhA, CV_3180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536 Chromobacterium violaceum])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tk2 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk2 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Phenylalanine hydroxylase (PAH) is a non-heme iron enzyme that catalyzes oxidation of phenylalanine to tyrosine, a reaction that must be kept under tight regulatory control. Mammalian PAH has a regulatory domain in which binding of the substrate leads to allosteric activation of the enzyme. However, the existence of PAH regulation in evolutionarily distant organisms, for example some bacteria in which it occurs, has so far been underappreciated. In an attempt to crystallographically characterize substrate binding by PAH from Chromobacterium violaceum, a single-domain monomeric enzyme, electron density for phenylalanine was observed at a distal site 15.7 A from the active site. Isothermal titration calorimetry (ITC) experiments revealed a dissociation constant of 24 +/- 1.1 muM for phenylalanine. Under the same conditions, ITC revealed no detectable binding for alanine, tyrosine, or isoleucine, indicating the distal site may be selective for phenylalanine. Point mutations of amino acid residues in the distal site that contact phenylalanine (F258A, Y155A, T254A) led to impaired binding, consistent with the presence of distal site binding in solution. Although kinetic analysis revealed that the distal site mutants suffer discernible loss of their catalytic activity, X-ray crystallographic analysis of Y155A and F258A, the two mutants with the most noticeable decrease in activity, revealed no discernible change in the structure of their active sites, suggesting that the effect of distal binding may result from protein dynamics in solution.

	~~==About this Structure==~~		An additional substrate binding site in a bacterial phenylalanine hydroxylase.,Ronau JA, Paul LN, Fuchs JE, Corn IR, Wagner KT, Liedl KR, Abu-Omar MM, Das C Eur Biophys J. 2013 Jul 17. PMID:23860686<ref>PMID:23860686</ref>
	~~[[3tk2]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Chromobacterium_violaceum Chromobacterium violaceum]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3TK2 OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	==See Also==		==See Also==
	*[[Phenylalanine hydroxylase\|Phenylalanine hydroxylase]]		*[[Phenylalanine hydroxylase\|Phenylalanine hydroxylase]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:023860686</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Chromobacterium violaceum]]		[[Category: Chromobacterium violaceum]]
	[[Category: Phenylalanine 4-monooxygenase]]		[[Category: Phenylalanine 4-monooxygenase]]
	[[Category: Abu-Omar, M M.]]		[[Category: Abu-Omar, M M]]
	[[Category: Das, C.]]		[[Category: Das, C]]
	[[Category: Ronau, J A.]]		[[Category: Ronau, J A]]
	[[Category: Beta]]		[[Category: Beta]]
	[[Category: Hydroxylase]]		[[Category: Hydroxylase]]

OCA at 03:27, 1 August 2013

2013-08-01T03:27:44Z

← Older revision		Revision as of 03:27, 1 August 2013
Line 1:		Line 1:
	~~[[Image:3tk2.jpg\|left\|200px]]~~

	{{STRUCTURE_3tk2\| PDB=3tk2 \| SCENE= }}		{{STRUCTURE_3tk2\| PDB=3tk2 \| SCENE= }}

	===Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site===		===Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site===
			{{ABSTRACT_PUBMED_23860686}}

	==About this Structure==		==About this Structure==
	[[3tk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chromobacterium_violaceum Chromobacterium violaceum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA].		[[3tk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chromobacterium_violaceum Chromobacterium violaceum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK2 OCA].

			==See Also==
			*[[Phenylalanine hydroxylase\|Phenylalanine hydroxylase]]

			==Reference==
			<ref group="xtra">PMID:023860686</ref><references group="xtra"/><references/>
	[[Category: Chromobacterium violaceum]]		[[Category: Chromobacterium violaceum]]
	[[Category: Phenylalanine 4-monooxygenase]]		[[Category: Phenylalanine 4-monooxygenase]]

OCA at 05:30, 5 September 2012

2012-09-05T05:30:24Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3tk2.jpg|left|200px]]
-The entry 3tk2 is ON HOLD  until Paper Publication
+{{STRUCTURE_3tk2|  PDB=3tk2  |  SCENE=  }}
-Authors: Ronau, J.A., Abu-Omar, M.M., Das, C.
+===Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site===
-Description: Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site

OCA at 05:33, 4 April 2012

2012-04-04T05:33:32Z

← Older revision		Revision as of 05:33, 4 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3tk2 is ON HOLD		The entry 3tk2 is ON HOLD until Paper Publication

	Authors: Ronau, J.A., Abu-Omar, M.M., Das, C.		Authors: Ronau, J.A., Abu-Omar, M.M., Das, C.

	Description: Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site		Description: Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site

OCA: Protected "3tk2" [edit=sysop:move=sysop]

2011-08-31T06:27:21Z

Protected "3tk2" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:27, 31 August 2011
(No difference)

OCA: New page: '''Unreleased structure''' The entry 3tk2 is ON HOLD Authors: Ronau, J.A., Abu-Omar, M.M., Das, C. Description: Crystallographic structure of phenylalanine hydroxylase from Chromobacte...

2011-08-31T06:27:20Z

New page: '''Unreleased structure''' The entry 3tk2 is ON HOLD Authors: Ronau, J.A., Abu-Omar, M.M., Das, C. Description: Crystallographic structure of phenylalanine hydroxylase from Chromobacte...

New page

'''Unreleased structure'''

The entry 3tk2 is ON HOLD

Authors: Ronau, J.A., Abu-Omar, M.M., Das, C.

Description: Crystallographic structure of phenylalanine hydroxylase from Chromobacterium violaceum cocrystallized with phenylalanine in a site distal to the active site