3tk7 - Revision history

OCA at 10:29, 6 November 2024

2024-11-06T10:29:01Z

← Older revision		Revision as of 10:29, 6 November 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q5NFX0_FRATT Q5NFX0_FRATT] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity).[RuleBase:RU004155][SAAS:SAAS004730_004_006516]		[https://www.uniprot.org/uniprot/Q5NFX0_FRATT Q5NFX0_FRATT] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity).[RuleBase:RU004155][SAAS:SAAS004730_004_006516]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The Burgi-Dunitz angle (alphaBD) describes the trajectory of approach of a nucleophile to an electrophile. The adoption of a stereoelectronically favorable alphaBD can necessitate significant reactive-group repositioning over the course of bond formation. In the context of enzyme catalysis, interactions with the protein constrain substrate rotation, which could necessitate structural transformations during bond formation. To probe this theoretical framework vis-a-vis biocatalysis, Schiff-base formation was analysed in Francisella tularensis transaldolase (TAL). Crystal structures of wild-type and Lys-->Met mutant TAL in covalent and noncovalent complexes with fructose 6-phosphate and sedoheptulose 7-phosphate clarify the mechanism of catalysis and reveal that substrate keto moieties undergo significant conformational changes during Schiff-base formation. Structural changes compelled by the trajectory considerations discussed here bear relevance to bond formation in a variety of constrained enzymic/engineered systems and can inform the design of covalent therapeutics.

			Adherence to Burgi-Dunitz stereochemical principles requires significant structural rearrangements in Schiff-base formation: insights from transaldolase complexes.,Light SH, Minasov G, Duban ME, Anderson WF Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):544-52. doi:, 10.1107/S1399004713030666. Epub 2014 Jan 31. PMID:24531488<ref>PMID:24531488</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3tk7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Transaldolase 3D structures\|Transaldolase 3D structures]]		*[[Transaldolase 3D structures\|Transaldolase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:31, 14 March 2024

2024-03-14T13:31:41Z

← Older revision		Revision as of 13:31, 14 March 2024
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	<StructureSection load='3tk7' size='340' side='right'caption='[[3tk7]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3tk7' size='340' side='right'caption='[[3tk7]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"francisella_tularensis_subsp~~.~~_nearctica"_olsufjev_1970 "francisella~~ tularensis subsp. ~~nearctica" olsufjev 1970~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TK7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._tularensis Francisella tularensis subsp. tularensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TK7 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=F6R:FRUCTOSE+~~-~~6-PHOSPHATE'>F6R</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3igx\|3igx]], [[3te9\|3te9]], [[4e0c\|4e0c]], [[3tno\|3tno]], [[3tkf\|3tkf]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>FTT_1093c, talA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~119856~~ "~~Francisella tularensis subsp. nearctica~~" ~~Olsufjev 1970])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [https://pdbe.org/3tk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [https://www.ebi.ac.uk/pdbsum/3tk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [https://pdbe.org/3tk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [https://www.ebi.ac.uk/pdbsum/3tk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Q5NFX0_FRATT Q5NFX0_FRATT]] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity).[RuleBase:RU004155][SAAS:SAAS004730_004_006516]		[https://www.uniprot.org/uniprot/Q5NFX0_FRATT Q5NFX0_FRATT] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity).[RuleBase:RU004155][SAAS:SAAS004730_004_006516]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The Burgi-Dunitz angle (alphaBD) describes the trajectory of approach of a nucleophile to an electrophile. The adoption of a stereoelectronically favorable alphaBD can necessitate significant reactive-group repositioning over the course of bond formation. In the context of enzyme catalysis, interactions with the protein constrain substrate rotation, which could necessitate structural transformations during bond formation. To probe this theoretical framework vis-a-vis biocatalysis, Schiff-base formation was analysed in Francisella tularensis transaldolase (TAL). Crystal structures of wild-type and Lys-->Met mutant TAL in covalent and noncovalent complexes with fructose 6-phosphate and sedoheptulose 7-phosphate clarify the mechanism of catalysis and reveal that substrate keto moieties undergo significant conformational changes during Schiff-base formation. Structural changes compelled by the trajectory considerations discussed here bear relevance to bond formation in a variety of constrained enzymic/engineered systems and can inform the design of covalent therapeutics.

	Adherence to Burgi-Dunitz stereochemical principles requires significant structural rearrangements in Schiff-base formation: insights from transaldolase complexes.,Light SH, Minasov G, Duban ME, Anderson WF Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):544-52. doi:, 10.1107/S1399004713030666. Epub 2014 Jan 31. PMID:24531488<ref>PMID:24531488</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3tk7" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Transaldolase 3D structures\|Transaldolase 3D structures]]		*[[Transaldolase 3D structures\|Transaldolase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Francisella tularensis subsp. ~~nearctica olsufjev 1970~~]]		[[Category: Francisella tularensis subsp. tularensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Transaldolase]]~~		[[Category: Anderson WF]]
	[[Category: Anderson~~, W F]]~~		[[Category: Halavaty AS]]
	~~[[Category: Structural genomic~~]]		[[Category: Light SH]]
	[[Category: Halavaty~~, A S~~]]		[[Category: Minasov G]]
	[[Category: Light~~, S H~~]]		[[Category: Papazisi L]]
	[[Category: Minasov, G]]		[[Category: Shuvalova L]]
	[[Category: Papazisi, L]]
	[[Category: Shuvalova, L]]
	~~[[Category: Alpha-beta barrel/tim barrel]]~~
	~~[[Category: Csgid]]~~
	~~[[Category: Transferase~~]]

OCA at 17:01, 6 July 2022

2022-07-06T17:01:49Z

← Older revision		Revision as of 17:01, 6 July 2022
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	==2.0 Angstrom Resolution Crystal Structure of Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate==		==2.0 Angstrom Resolution Crystal Structure of Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate==
	<StructureSection load='3tk7' size='340' side='right' caption='[[3tk7]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3tk7' size='340' side='right'caption='[[3tk7]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tk7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"francisella_tularensis_subsp._nearctica"_olsufjev_1970 "francisella tularensis subsp. nearctica" olsufjev 1970]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TK7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"francisella_tularensis_subsp._nearctica"_olsufjev_1970 "francisella tularensis subsp. nearctica" olsufjev 1970]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TK7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3igx\|3igx]], [[3te9\|3te9]], [[4e0c\|4e0c]], [[3tno\|3tno]], [[3tkf\|3tkf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3igx\|3igx]], [[3te9\|3te9]], [[4e0c\|4e0c]], [[3tno\|3tno]], [[3tkf\|3tkf]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTT_1093c, talA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119856 "Francisella tularensis subsp. nearctica" Olsufjev 1970])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTT_1093c, talA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119856 "Francisella tularensis subsp. nearctica" Olsufjev 1970])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [~~http~~://pdbe.org/3tk7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tk7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [https://pdbe.org/3tk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [https://www.ebi.ac.uk/pdbsum/3tk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/Q5NFX0_FRATT Q5NFX0_FRATT]] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity).[RuleBase:RU004155][SAAS:SAAS004730_004_006516]		[[https://www.uniprot.org/uniprot/Q5NFX0_FRATT Q5NFX0_FRATT]] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity).[RuleBase:RU004155][SAAS:SAAS004730_004_006516]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 3tk7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3tk7" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Transaldolase 3D structures\|Transaldolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Francisella tularensis subsp. nearctica olsufjev 1970]]		[[Category: Francisella tularensis subsp. nearctica olsufjev 1970]]
			[[Category: Large Structures]]
	[[Category: Transaldolase]]		[[Category: Transaldolase]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]

OCA at 07:32, 8 November 2017

2017-11-08T07:32:28Z

← Older revision		Revision as of 07:32, 8 November 2017
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 3tk7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3tk7" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Transaldolase\|Transaldolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:13, 5 August 2016

2016-08-05T01:13:52Z

← Older revision		Revision as of 01:13, 5 August 2016
Line 1:		Line 1:

	==2.0 Angstrom Resolution Crystal Structure of Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate==		==2.0 Angstrom Resolution Crystal Structure of Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate==
	<StructureSection load='3tk7' size='340' side='right' caption='[[3tk7]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3tk7' size='340' side='right' caption='[[3tk7]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTT_1093c, talA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119856 "Francisella tularensis subsp. nearctica" Olsufjev 1970])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTT_1093c, talA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119856 "Francisella tularensis subsp. nearctica" Olsufjev 1970])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [http://pdbe.org/3tk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tk7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3tk7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 21:31, 24 December 2014

2014-12-24T21:31:01Z

← Older revision		Revision as of 21:31, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"francisella_tularensis_subsp._nearctica"_olsufjev_1970 "francisella tularensis subsp. nearctica" olsufjev 1970]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TK7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"francisella_tularensis_subsp._nearctica"_olsufjev_1970 "francisella tularensis subsp. nearctica" olsufjev 1970]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TK7 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3igx\|3igx]], [[3te9\|3te9]], [[4e0c\|4e0c]], [[3tno\|3tno]], [[3tkf\|3tkf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3igx\|3igx]], [[3te9\|3te9]], [[4e0c\|4e0c]], [[3tno\|3tno]], [[3tkf\|3tkf]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTT_1093c, talA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119856 "Francisella tularensis subsp. nearctica" Olsufjev 1970])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTT_1093c, talA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119856 "Francisella tularensis subsp. nearctica" Olsufjev 1970])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk7 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q5NFX0_FRATT Q5NFX0_FRATT]] Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity).[RuleBase:RU004155][SAAS:SAAS004730_004_006516]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Adherence to Burgi-Dunitz stereochemical principles requires significant structural rearrangements in Schiff-base formation: insights from transaldolase complexes.,Light SH, Minasov G, Duban ME, Anderson WF Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):544-52. doi:, 10.1107/S1399004713030666. Epub 2014 Jan 31. PMID:24531488<ref>PMID:24531488</ref>		Adherence to Burgi-Dunitz stereochemical principles requires significant structural rearrangements in Schiff-base formation: insights from transaldolase complexes.,Light SH, Minasov G, Duban ME, Anderson WF Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):544-52. doi:, 10.1107/S1399004713030666. Epub 2014 Jan 31. PMID:24531488<ref>PMID:24531488</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

			==See Also==
			*[[Transaldolase\|Transaldolase]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 28:
	[[Category: Francisella tularensis subsp. nearctica olsufjev 1970]]		[[Category: Francisella tularensis subsp. nearctica olsufjev 1970]]
	[[Category: Transaldolase]]		[[Category: Transaldolase]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F]]
	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]		[[Category: Structural genomic]]
	[[Category: Halavaty, A S.]]		[[Category: Halavaty, A S]]
	[[Category: Light, S H.]]		[[Category: Light, S H]]
	[[Category: Minasov, G.]]		[[Category: Minasov, G]]
	[[Category: Papazisi, L.]]		[[Category: Papazisi, L]]
	[[Category: Shuvalova, L.]]		[[Category: Shuvalova, L]]
	[[Category: Alpha-beta barrel/tim barrel]]		[[Category: Alpha-beta barrel/tim barrel]]
	~~[[Category: Center for structural genomics of infectious disease]]~~
	[[Category: Csgid]]		[[Category: Csgid]]
	~~[[Category: Structural genomic]]~~
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 13:17, 18 May 2014

2014-05-18T13:17:27Z

← Older revision		Revision as of 13:17, 18 May 2014
Line 1:		Line 1:
	[[~~Image~~:~~3tk7~~.~~jpg~~\|~~left~~\|~~200px~~]]		==2.0 Angstrom Resolution Crystal Structure of Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate==
			<StructureSection load='3tk7' size='340' side='right' caption='[[3tk7]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3tk7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"francisella_tularensis_subsp._nearctica"_olsufjev_1970 "francisella tularensis subsp. nearctica" olsufjev 1970]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TK7 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3igx\|3igx]], [[3te9\|3te9]], [[4e0c\|4e0c]], [[3tno\|3tno]], [[3tkf\|3tkf]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTT_1093c, talA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119856 "Francisella tularensis subsp. nearctica" Olsufjev 1970])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transaldolase Transaldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.2 2.2.1.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/3tk7 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The Burgi-Dunitz angle (alphaBD) describes the trajectory of approach of a nucleophile to an electrophile. The adoption of a stereoelectronically favorable alphaBD can necessitate significant reactive-group repositioning over the course of bond formation. In the context of enzyme catalysis, interactions with the protein constrain substrate rotation, which could necessitate structural transformations during bond formation. To probe this theoretical framework vis-a-vis biocatalysis, Schiff-base formation was analysed in Francisella tularensis transaldolase (TAL). Crystal structures of wild-type and Lys-->Met mutant TAL in covalent and noncovalent complexes with fructose 6-phosphate and sedoheptulose 7-phosphate clarify the mechanism of catalysis and reveal that substrate keto moieties undergo significant conformational changes during Schiff-base formation. Structural changes compelled by the trajectory considerations discussed here bear relevance to bond formation in a variety of constrained enzymic/engineered systems and can inform the design of covalent therapeutics.

	<!--		Adherence to Burgi-Dunitz stereochemical principles requires significant structural rearrangements in Schiff-base formation: insights from transaldolase complexes.,Light SH, Minasov G, Duban ME, Anderson WF Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):544-52. doi:, 10.1107/S1399004713030666. Epub 2014 Jan 31. PMID:24531488<ref>PMID:24531488</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3tk7"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded~~),
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3tk7\| PDB=3tk7 \| SCENE= }}~~

	~~===2~~.~~0 Angstrom Resolution Crystal Structure~~ of ~~Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	==~~About this Structure~~==		<references/>
	~~[[3tk7]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Francisella_tularensis_subsp._tularensis Francisella tularensis subsp. tularensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TK7 OCA].		__TOC__
	[[Category: Francisella tularensis subsp. ~~tularensis~~]]		</StructureSection>
			[[Category: Francisella tularensis subsp. nearctica olsufjev 1970]]
	[[Category: Transaldolase]]		[[Category: Transaldolase]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]

OCA at 06:22, 7 September 2011

2011-09-07T06:22:47Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3tk7.jpg|left|200px]]
-The entry 3tk7 is ON HOLD
+<!--
-Authors: Light, S.H., Minasov, G., Halavaty, A.S., Shuvalova, L., Papazisi, L., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)
+===2.0 Angstrom Resolution Crystal Structure of Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate===
-Description: 2.0 Angstrom Resolution Crystal Structure of Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate

OCA: Protected "3tk7" [edit=sysop:move=sysop]

2011-08-31T06:27:30Z

Protected "3tk7" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:27, 31 August 2011
(No difference)

OCA: New page: '''Unreleased structure''' The entry 3tk7 is ON HOLD Authors: Light, S.H., Minasov, G., Halavaty, A.S., Shuvalova, L., Papazisi, L., Anderson, W.F., Center for Structural Genomics of In...

2011-08-31T06:27:29Z

New page: '''Unreleased structure''' The entry 3tk7 is ON HOLD Authors: Light, S.H., Minasov, G., Halavaty, A.S., Shuvalova, L., Papazisi, L., Anderson, W.F., Center for Structural Genomics of In...

New page

'''Unreleased structure'''

The entry 3tk7 is ON HOLD

Authors: Light, S.H., Minasov, G., Halavaty, A.S., Shuvalova, L., Papazisi, L., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: 2.0 Angstrom Resolution Crystal Structure of Transaldolase B (TalA) from Francisella tularensis in Covalent Complex with Fructose 6-Phosphate