3tle - Revision history

OCA at 13:32, 14 March 2024

2024-03-14T13:32:48Z

← Older revision		Revision as of 13:32, 14 March 2024
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	<StructureSection load='3tle' size='340' side='right'caption='[[3tle]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3tle' size='340' side='right'caption='[[3tle]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLE FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1,2~~-~~ETHANEDIOL'>EDO</scene>~~, ~~<scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tlg\|3tlg]], [[3tly\|3tly]], [[3tlz\|3tlz]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">mccF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [https://pdbe.org/3tle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [https://www.ebi.ac.uk/pdbsum/3tle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tle ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [https://pdbe.org/3tle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [https://www.ebi.ac.uk/pdbsum/3tle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tle ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/MCCF_ECOLX MCCF_ECOLX] Involved in specific self-immunity to microcin C7.
	Several classes of naturally occurring antimicrobials exert their antibiotic activity by specifically targeting aminoacyl-tRNA synthetases, validating these enzymes as drug targets. The aspartyl tRNA synthetase "Trojan horse" inhibitor microcin C7 (McC7) consists of a nonhydrolyzable aspartyl-adenylate conjugated to a hexapeptide carrier that facilitates active import into bacterial cells through an oligopeptide transport system. ~~Subsequent proteolytic processing releases the toxic compound inside the cell~~. Producing strains of McC7 must protect themselves against autotoxicity that may result from premature processing. The mccF gene confers resistance against endogenous and exogenous McC7 by hydrolyzing the amide bond that connects the peptide and nucleotide moieties of McC7. We present here crystal structures of MccF, in ~~complex with various ligands. The MccF structure is similar to that of dipeptide ld~~-~~carboxypeptidase, but with an additional loop proximal~~ to the active site that serves as the primary determinant for recognition of adenylated substrates. Wild-type MccF only hydrolyzes the naturally occurring aspartyl phosphoramidate McC7 and synthetic peptidyl sulfamoyl adenylates that contain anionic side chains. We show that substitutions of two active site MccF residues result in a specificity switch toward aromatic aminoacyl-adenylate substrates. These results suggest how MccF-like enzymes may be used to avert various toxic aminoacyl-adenylates that accumulate during antibiotic biosynthesis or in normal metabolism of the cell.

	~~Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic~~ microcin C7.~~,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3tle" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Agarwal, V]]		[[Category: Agarwal V]]
	[[Category: Nair~~, S K]]~~		[[Category: Nair SK]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Serine protease~~]]

OCA at 17:03, 6 July 2022

2022-07-06T17:03:06Z

← Older revision		Revision as of 17:03, 6 July 2022
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	==Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate==		==Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate==
	<StructureSection load='3tle' size='340' side='right' caption='[[3tle]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3tle' size='340' side='right'caption='[[3tle]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tlg\|3tlg]], [[3tly\|3tly]], [[3tlz\|3tlz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tlg\|3tlg]], [[3tly\|3tly]], [[3tlz\|3tlz]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [~~http~~://pdbe.org/3tle PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tle PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tle ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [https://pdbe.org/3tle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [https://www.ebi.ac.uk/pdbsum/3tle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tle ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
			[[Category: Large Structures]]
	[[Category: Agarwal, V]]		[[Category: Agarwal, V]]
	[[Category: Nair, S K]]		[[Category: Nair, S K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 10:48, 11 August 2016

2016-08-11T10:48:10Z

← Older revision		Revision as of 10:48, 11 August 2016
Line 1:		Line 1:

	==Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate==		==Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate==
	<StructureSection load='3tle' size='340' side='right' caption='[[3tle]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3tle' size='340' side='right' caption='[[3tle]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tlg\|3tlg]], [[3tly\|3tly]], [[3tlz\|3tlz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tlg\|3tlg]], [[3tly\|3tly]], [[3tlz\|3tlz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [http://www.ebi.ac.uk/pdbsum/3tle PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [http://pdbe.org/3tle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [http://www.ebi.ac.uk/pdbsum/3tle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tle ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3tle" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Escherichia~~ coli]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Agarwal, V]]		[[Category: Agarwal, V]]
	[[Category: Nair, S K]]		[[Category: Nair, S K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 09:17, 4 January 2015

2015-01-04T09:17:00Z

← Older revision		Revision as of 09:17, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLE FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tlg\|3tlg]], [[3tly\|3tly]], [[3tlz\|3tlz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tlg\|3tlg]], [[3tly\|3tly]], [[3tlz\|3tlz]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [http://www.ebi.ac.uk/pdbsum/3tle PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [http://www.ebi.ac.uk/pdbsum/3tle PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>		Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Agarwal, V.]]		[[Category: Agarwal, V]]
	[[Category: Nair, S K.]]		[[Category: Nair, S K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 05:49, 5 June 2014

2014-06-05T05:49:44Z

← Older revision		Revision as of 05:49, 5 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~3tle~~.~~png~~\|~~left~~\|~~200px~~]]		==Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate==
			<StructureSection load='3tle' size='340' side='right' caption='[[3tle]], [[Resolution\|resolution]] 1.30Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3tle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLE FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSU:O5-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE'>GSU</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tlg\|3tlg]], [[3tly\|3tly]], [[3tlz\|3tlz]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tle RCSB], [http://www.ebi.ac.uk/pdbsum/3tle PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Several classes of naturally occurring antimicrobials exert their antibiotic activity by specifically targeting aminoacyl-tRNA synthetases, validating these enzymes as drug targets. The aspartyl tRNA synthetase "Trojan horse" inhibitor microcin C7 (McC7) consists of a nonhydrolyzable aspartyl-adenylate conjugated to a hexapeptide carrier that facilitates active import into bacterial cells through an oligopeptide transport system. Subsequent proteolytic processing releases the toxic compound inside the cell. Producing strains of McC7 must protect themselves against autotoxicity that may result from premature processing. The mccF gene confers resistance against endogenous and exogenous McC7 by hydrolyzing the amide bond that connects the peptide and nucleotide moieties of McC7. We present here crystal structures of MccF, in complex with various ligands. The MccF structure is similar to that of dipeptide ld-carboxypeptidase, but with an additional loop proximal to the active site that serves as the primary determinant for recognition of adenylated substrates. Wild-type MccF only hydrolyzes the naturally occurring aspartyl phosphoramidate McC7 and synthetic peptidyl sulfamoyl adenylates that contain anionic side chains. We show that substitutions of two active site MccF residues result in a specificity switch toward aromatic aminoacyl-adenylate substrates. These results suggest how MccF-like enzymes may be used to avert various toxic aminoacyl-adenylates that accumulate during antibiotic biosynthesis or in normal metabolism of the cell.

	~~<!--~~		Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3tle"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter~~ (~~which sets the initial scene displayed when the page is loaded~~),
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3tle\| PDB=3tle \| SCENE= }}~~

	~~===Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	~~<!--~~		<references/>
	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22388748}}, adds the Publication Abstract to~~ the ~~page~~		__TOC__
	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22388748 is the PubMed ID number~~.		</StructureSection>
	-->
	~~{{ABSTRACT_PUBMED_22388748}}~~

	~~==About this Structure==~~
	~~[[3tle]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA].~~

	==~~Reference~~==
	<~~ref group="xtra">PMID:022388748<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Agarwal, V.]]		[[Category: Agarwal, V.]]

OCA at 06:34, 28 March 2012

2012-03-28T06:34:30Z

← Older revision		Revision as of 06:34, 28 March 2012
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	[[Image:3tle.~~jpg~~\|left\|200px]]		[[Image:3tle.png\|left\|200px]]

	<!--		<!--
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	===Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate===		===Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22388748}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22388748 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22388748}}

	==About this Structure==		==About this Structure==
	[[3tle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA].		[[3tle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA].

			==Reference==
			<ref group="xtra">PMID:022388748</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Agarwal, V.]]		[[Category: Agarwal, V.]]

OCA at 08:59, 29 February 2012

2012-02-29T08:59:49Z

← Older revision		Revision as of 08:59, 29 February 2012
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	~~'''Unreleased structure'''~~		[[Image:3tle.jpg\|left\|200px]]

	The ~~entry~~ 3tle ~~is ON HOLD~~ ~~until Paper Publication~~		<!--
			The line below this paragraph, containing "STRUCTURE_3tle", creates the "Structure Box" on the page.
			You may change the PDB parameter (which sets the PDB file loaded into the applet)
			or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
			or leave the SCENE parameter empty for the default display.
			-->
			{{STRUCTURE_3tle\| PDB=3tle \| SCENE= }}

	~~Authors: Agarwal, V., Nair, S.K.~~		===Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate===

	~~Description~~: ~~Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate~~
			==About this Structure==
			[[3tle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLE OCA].
			[[Category: Escherichia coli]]
			[[Category: Agarwal, V.]]
			[[Category: Nair, S K.]]
			[[Category: Hydrolase]]
			[[Category: Serine protease]]

OCA at 06:09, 5 October 2011

2011-10-05T06:09:32Z

← Older revision		Revision as of 06:09, 5 October 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3tle is ON HOLD		The entry 3tle is ON HOLD until Paper Publication

	Authors: Agarwal, V., Nair, S.K.		Authors: Agarwal, V., Nair, S.K.

	Description: Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate		Description: Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate

OCA: Protected "3tle" [edit=sysop:move=sysop]

2011-09-07T05:49:52Z

Protected "3tle" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:49, 7 September 2011
(No difference)

OCA: New page: '''Unreleased structure''' The entry 3tle is ON HOLD Authors: Agarwal, V., Nair, S.K. Description: Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate

2011-09-07T05:49:51Z

New page: '''Unreleased structure''' The entry 3tle is ON HOLD Authors: Agarwal, V., Nair, S.K. Description: Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate

New page

'''Unreleased structure'''

The entry 3tle is ON HOLD

Authors: Agarwal, V., Nair, S.K.

Description: Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate