3tp2 - Revision history

OCA at 10:10, 1 March 2024

2024-03-01T10:10:33Z

← Older revision		Revision as of 10:10, 1 March 2024
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	<StructureSection load='3tp2' size='340' side='right'caption='[[3tp2]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3tp2' size='340' side='right'caption='[[3tp2]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CWC2, NTC40, SLC3, YDL209C, D1041 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=4932 ATCC 18824])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [https://pdbe.org/3tp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [https://www.ebi.ac.uk/pdbsum/3tp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tp2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [https://pdbe.org/3tp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [https://www.ebi.ac.uk/pdbsum/3tp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tp2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/CWC2_YEAST CWC2_YEAST]] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.<ref>PMID:19435883</ref> <ref>PMID:22246180</ref>		[https://www.uniprot.org/uniprot/CWC2_YEAST CWC2_YEAST] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.<ref>PMID:19435883</ref> <ref>PMID:22246180</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The yeast splicing factor Cwc2 contacts several catalytically important RNA elements in the active spliceosome, suggesting that Cwc2 is involved in determining their spatial arrangement at the spliceosome's catalytic centre. We have determined the crystal structure of the Cwc2 functional core, revealing how a previously uncharacterized Torus domain, an RNA recognition motif (RRM) and a zinc finger (ZnF) are tightly integrated in a compact folding unit. The ZnF plays a pivotal role in the architecture of the whole assembly. UV-induced crosslinking of Cwc2-U6 snRNA allowed the identification by mass spectrometry of six RNA-contacting sites: four in or close to the RRM domain, one in the ZnF and one on a protruding element connecting the Torus and RRM domains. The three distinct regions contacting RNA are connected by a contiguous and conserved positively charged surface, suggesting an expanded interface for RNA accommodation. Cwc2 mutations confirmed that the connector element plays a crucial role in splicing. We conclude that Cwc2 acts as a multipartite RNA-binding platform to bring RNA elements of the spliceosome's catalytic centre into an active conformation.

	Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein.,Schmitzova J, Rasche N, Dybkov O, Kramer K, Fabrizio P, Urlaub H, Luhrmann R, Pena V EMBO J. 2012 Mar 9. doi: 10.1038/emboj.2012.58. PMID:22407296<ref>PMID:22407296</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3tp2" style="background-color:#fffaf0;"></div~~>

	==See Also==		==See Also==
Line 26:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 18824]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Schmitzova, J~~]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: ~~Ccch zinc finger]]~~		[[Category: Schmitzova J]]
	~~[[Category: Rna scaffold]]~~
	~~[[Category: Rrm domain]]~~
	~~[[Category: Splicing]]~~
	~~[[Category: Torus domain~~]]

OCA at 17:07, 6 July 2022

2022-07-06T17:07:22Z

← Older revision		Revision as of 17:07, 6 July 2022
Line 1:		Line 1:

	==Crystal Structure of the Splicing Factor Cwc2 from yeast==		==Crystal Structure of the Splicing Factor Cwc2 from yeast==
	<StructureSection load='3tp2' size='340' side='right' caption='[[3tp2]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3tp2' size='340' side='right'caption='[[3tp2]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWC2, NTC40, SLC3, YDL209C, D1041 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWC2, NTC40, SLC3, YDL209C, D1041 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [~~http~~://pdbe.org/3tp2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tp2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tp2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [https://pdbe.org/3tp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [https://www.ebi.ac.uk/pdbsum/3tp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tp2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/CWC2_YEAST CWC2_YEAST]] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.<ref>PMID:19435883</ref> <ref>PMID:22246180</ref>		[[https://www.uniprot.org/uniprot/CWC2_YEAST CWC2_YEAST]] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.<ref>PMID:19435883</ref> <ref>PMID:22246180</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
	*[[Pre-mRNA-splicing ~~factor~~\|Pre-mRNA-splicing ~~factor~~]]		*[[Pre-mRNA splicing factors 3D structures\|Pre-mRNA splicing factors 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
			[[Category: Large Structures]]
	[[Category: Schmitzova, J]]		[[Category: Schmitzova, J]]
	[[Category: Ccch zinc finger]]		[[Category: Ccch zinc finger]]

OCA at 19:24, 4 August 2016

2016-08-04T19:24:13Z

← Older revision		Revision as of 19:24, 4 August 2016
Line 1:		Line 1:

	==Crystal Structure of the Splicing Factor Cwc2 from yeast==		==Crystal Structure of the Splicing Factor Cwc2 from yeast==
	<StructureSection load='3tp2' size='340' side='right' caption='[[3tp2]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3tp2' size='340' side='right' caption='[[3tp2]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWC2, NTC40, SLC3, YDL209C, D1041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWC2, NTC40, SLC3, YDL209C, D1041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3tp2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [http://pdbe.org/3tp2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3tp2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tp2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3tp2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Saccharomyces cerevisiae~~]]		[[Category: Atcc 18824]]
	[[Category: Schmitzova, J]]		[[Category: Schmitzova, J]]
	[[Category: Ccch zinc finger]]		[[Category: Ccch zinc finger]]

OCA at 18:43, 24 December 2014

2014-12-24T18:43:02Z

← Older revision		Revision as of 18:43, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWC2, NTC40, SLC3, YDL209C, D1041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWC2, NTC40, SLC3, YDL209C, D1041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3tp2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3tp2 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CWC2_YEAST CWC2_YEAST]] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.<ref>PMID:19435883</ref> <ref>PMID:22246180</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein.,Schmitzova J, Rasche N, Dybkov O, Kramer K, Fabrizio P, Urlaub H, Luhrmann R, Pena V EMBO J. 2012 Mar 9. doi: 10.1038/emboj.2012.58. PMID:22407296<ref>PMID:22407296</ref>		Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein.,Schmitzova J, Rasche N, Dybkov O, Kramer K, Fabrizio P, Urlaub H, Luhrmann R, Pena V EMBO J. 2012 Mar 9. doi: 10.1038/emboj.2012.58. PMID:22407296<ref>PMID:22407296</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

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	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Schmitzova, J.]]		[[Category: Schmitzova, J]]
	[[Category: Ccch zinc finger]]		[[Category: Ccch zinc finger]]
	[[Category: Rna scaffold]]		[[Category: Rna scaffold]]

OCA at 06:06, 5 June 2014

2014-06-05T06:06:19Z

← Older revision		Revision as of 06:06, 5 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~3tp2~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of the Splicing Factor Cwc2 from yeast==
			<StructureSection load='3tp2' size='340' side='right' caption='[[3tp2]], [[Resolution\|resolution]] 2.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TP2 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWC2, NTC40, SLC3, YDL209C, D1041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3tp2 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The yeast splicing factor Cwc2 contacts several catalytically important RNA elements in the active spliceosome, suggesting that Cwc2 is involved in determining their spatial arrangement at the spliceosome's catalytic centre. We have determined the crystal structure of the Cwc2 functional core, revealing how a previously uncharacterized Torus domain, an RNA recognition motif (RRM) and a zinc finger (ZnF) are tightly integrated in a compact folding unit. The ZnF plays a pivotal role in the architecture of the whole assembly. UV-induced crosslinking of Cwc2-U6 snRNA allowed the identification by mass spectrometry of six RNA-contacting sites: four in or close to the RRM domain, one in the ZnF and one on a protruding element connecting the Torus and RRM domains. The three distinct regions contacting RNA are connected by a contiguous and conserved positively charged surface, suggesting an expanded interface for RNA accommodation. Cwc2 mutations confirmed that the connector element plays a crucial role in splicing. We conclude that Cwc2 acts as a multipartite RNA-binding platform to bring RNA elements of the spliceosome's catalytic centre into an active conformation.

	<!--		Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein.,Schmitzova J, Rasche N, Dybkov O, Kramer K, Fabrizio P, Urlaub H, Luhrmann R, Pena V EMBO J. 2012 Mar 9. doi: 10.1038/emboj.2012.58. PMID:22407296<ref>PMID:22407296</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3tp2"~~, ~~creates the "Structure Box" on the page~~.
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	~~{{STRUCTURE_3tp2\| PDB=3tp2 \| SCENE= }}~~

	~~===Crystal Structure~~ of the ~~Splicing Factor Cwc2 from yeast===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>

			==See Also==
	~~<!--~~		*[[Pre-mRNA-splicing factor\|Pre-mRNA-splicing factor]]
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22407296}}, adds the Publication Abstract to the page~~		== References ==
	~~(as it appears on PubMed at http://www.pubmed.gov), where 22407296 is the PubMed ID number.~~		<references/>
	~~-->~~		__TOC__
	~~{{ABSTRACT_PUBMED_22407296}}~~		</StructureSection>

	==~~About this Structure~~==
	[[~~3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA~~].

	==~~Reference~~==
	<~~ref group="xtra">PMID:022407296<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Schmitzova, J.]]		[[Category: Schmitzova, J.]]

OCA at 05:16, 4 April 2012

2012-04-04T05:16:36Z

← Older revision		Revision as of 05:16, 4 April 2012
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	[[Image:3tp2.~~jpg~~\|left\|200px]]		[[Image:3tp2.png\|left\|200px]]

	<!--		<!--
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	===Crystal Structure of the Splicing Factor Cwc2 from yeast===		===Crystal Structure of the Splicing Factor Cwc2 from yeast===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22407296}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22407296 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22407296}}

	==About this Structure==		==About this Structure==
	[[3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA].		[[3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA].

			==Reference==
			<ref group="xtra">PMID:022407296</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Schmitzova, J.]]		[[Category: Schmitzova, J.]]

OCA at 06:43, 14 March 2012

2012-03-14T06:43:40Z

← Older revision		Revision as of 06:43, 14 March 2012
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	~~'''Unreleased structure'''~~		[[Image:3tp2.jpg\|left\|200px]]

	The ~~entry~~ 3tp2 ~~is ON HOLD~~ ~~until Paper Publication~~		<!--
			The line below this paragraph, containing "STRUCTURE_3tp2", creates the "Structure Box" on the page.
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			or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
			or leave the SCENE parameter empty for the default display.
			-->
			{{STRUCTURE_3tp2\| PDB=3tp2 \| SCENE= }}

	~~Authors: Schmitzova, J.~~		===Crystal Structure of the Splicing Factor Cwc2 from yeast===

	~~Description~~: ~~Crystal Structure of the~~ Splicing ~~Factor Cwc2 from yeast~~
			==About this Structure==
			[[3tp2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TP2 OCA].
			[[Category: Saccharomyces cerevisiae]]
			[[Category: Schmitzova, J.]]
			[[Category: Ccch zinc finger]]
			[[Category: Rna scaffold]]
			[[Category: Rrm domain]]
			[[Category: Splicing]]
			[[Category: Torus domain]]

OCA at 08:41, 30 October 2011

2011-10-30T08:41:51Z

← Older revision		Revision as of 08:41, 30 October 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3tp2 is ON HOLD		The entry 3tp2 is ON HOLD until Paper Publication

	Authors: Schmitzova, J.		Authors: Schmitzova, J.

	Description: Crystal Structure of the Splicing Factor Cwc2 from yeast		Description: Crystal Structure of the Splicing Factor Cwc2 from yeast

OCA: Protected "3tp2" [edit=sysop:move=sysop]

2011-09-14T06:06:57Z

Protected "3tp2" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:06, 14 September 2011
(No difference)

OCA: New page: '''Unreleased structure''' The entry 3tp2 is ON HOLD Authors: Schmitzova, J. Description: Crystal Structure of the Splicing Factor Cwc2 from yeast

2011-09-14T06:06:55Z

New page: '''Unreleased structure''' The entry 3tp2 is ON HOLD Authors: Schmitzova, J. Description: Crystal Structure of the Splicing Factor Cwc2 from yeast

New page

'''Unreleased structure'''

The entry 3tp2 is ON HOLD

Authors: Schmitzova, J.

Description: Crystal Structure of the Splicing Factor Cwc2 from yeast