3vsz - Revision history

OCA at 07:36, 11 February 2026

2026-02-11T07:36:56Z

← Older revision		Revision as of 07:36, 11 February 2026
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A3DD67_ACET2 A3DD67_ACET2]		[https://www.uniprot.org/uniprot/A3DD67_ACET2 A3DD67_ACET2]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Glycoside hydrolase family 43 (GH43) consists of a variety of enzymes distributed widely in prokaryotes and eukaryotes. The mechanism by which GH43 enzymes hydrolyze oligosaccharides requires three essential acidic amino acid residues. However, one of them is thought to be missing in galactan beta-1,3-galactosidases from the GH43 family. Ct1,3Gal43A, from Clostridium thermocellum, is comprised of a GH43 domain, a CBM13 domain, and a dockerin domain and exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch. Here, we present its crystal structure at 2.7 A resolution and complex structures of the enzyme with several substrates and analogs. Two modes of substrate binding were observed at the beta site of the CtCBM13 domain, and one galactobiose molecule was found in an "L" shaped pocket of the CtGH43 domain, which appears large enough to accommodate two more galactose units. In addition, we found that mutating Glu112 to Gln or Ala eliminated the galactan hydrolysis activity of Ct1,3Gal43A while did not disrupt its ligand binding ability. Combining this results and the crystal structure we identified Glu112 in Ct1,3Gal43A as the 'missing' essential acidic residue in galactan beta-1,3-galactosidases. Structural information presented here also suggests a mechanism by which Ct1,3Gal43A bypasses beta-1,6 linked branches in the substrate and another mechanism by which the substrate is delivered 'in trans' from the CBM13 domain to the catalytic GH43 domain.

			Crystal structure of 1,3Gal43A, an exo-beta-1,3-galactanase from Clostridium thermocellum.,Jiang D, Fan J, Wang X, Zhao Y, Huang B, Liu J, Zhang XC J Struct Biol. 2012 Dec;180(3):447-57. doi: 10.1016/j.jsb.2012.08.005. Epub 2012 , Aug 29. PMID:22960181<ref>PMID:22960181</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3vsz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ricin 3D structures\|Ricin 3D structures]]		*[[Ricin 3D structures\|Ricin 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:41, 20 March 2024

2024-03-20T08:41:49Z

← Older revision		Revision as of 08:41, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3vsz' size='340' side='right'caption='[[3vsz]], [[Resolution\|resolution]] 2.89Å' scene=''>		<StructureSection load='3vsz' size='340' side='right'caption='[[3vsz]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3vsz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Acet2 Acet2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VSZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3vsz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus_ATCC_27405 Acetivibrio thermocellus ATCC 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VSZ FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.893Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3vsf\|3vsf]], [[3vt0\|3vt0]], [[3vt1\|3vt1]], [[3vt2\|3vt2]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~Cthe_0661 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 ACET2])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vsz OCA], [https://pdbe.org/3vsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vsz RCSB], [https://www.ebi.ac.uk/pdbsum/3vsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vsz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vsz OCA], [https://pdbe.org/3vsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vsz RCSB], [https://www.ebi.ac.uk/pdbsum/3vsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vsz ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	=~~= Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/A3DD67_ACET2 A3DD67_ACET2]
	Glycoside hydrolase family 43 (GH43) consists of a variety of enzymes distributed widely in prokaryotes and eukaryotes. The mechanism by which GH43 enzymes hydrolyze oligosaccharides requires three essential acidic amino acid residues. However, one of them is thought to be missing in galactan beta-1,3-galactosidases from the GH43 family. Ct1,3Gal43A, from Clostridium thermocellum, is comprised of a GH43 domain, a CBM13 domain, and a dockerin domain and exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch. Here, we present its crystal structure at 2.7 A resolution and complex structures of the enzyme with several substrates and analogs. Two modes of substrate binding were observed at the beta site of the CtCBM13 domain, and one galactobiose molecule was found in an "L" shaped pocket of the CtGH43 domain, which appears large enough to accommodate two more galactose units. In addition, we found that mutating Glu112 to Gln or Ala eliminated the galactan hydrolysis activity of Ct1,3Gal43A while did not disrupt its ligand binding ability. Combining this results and the crystal structure we identified Glu112 in Ct1,3Gal43A as the 'missing' essential acidic residue in galactan beta-1,3-galactosidases. Structural information presented here also suggests a mechanism by which Ct1,3Gal43A bypasses beta-1,6 linked branches in the substrate and another mechanism by which the substrate is delivered 'in trans' from the CBM13 domain to the catalytic GH43 domain.

	~~Crystal structure of 1,3Gal43A, an exo-beta-1,3-galactanase from Clostridium thermocellum.,Jiang D, Fan J, Wang X, Zhao Y, Huang B, Liu J, Zhang XC J Struct Biol. 2012 Dec;180(3):447-57. doi~~: ~~10.1016/j.jsb.2012.08.005. Epub 2012 , Aug 29. PMID:22960181<ref>PMID:22960181<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 3vsz" style="background-color:#fffaf0;"><~~/~~div>~~

	==See Also==		==See Also==
	*[[Ricin 3D structures\|Ricin 3D structures]]		*[[Ricin 3D structures\|Ricin 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Acet2~~]]		[[Category: Acetivibrio thermocellus ATCC 27405]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Fan, J]]		[[Category: Fan J]]
	[[Category: Huang, B]]		[[Category: Huang B]]
	[[Category: Jiang, D]]		[[Category: Jiang D]]
	[[Category: Wang, X]]		[[Category: Wang X]]
	[[Category: Zhang~~, X C~~]]		[[Category: Zhang XC]]
	[[Category: Zhao, Y]]		[[Category: Zhao Y]]
	~~[[Category: Cbm13]]~~
	~~[[Category: Galactan hydrolysis]]~~
	~~[[Category: Gh43]]~~
	~~[[Category: Sugar binding protein~~]]

OCA at 19:02, 27 July 2022

2022-07-27T19:02:33Z

← Older revision		Revision as of 19:02, 27 July 2022
Line 1:		Line 1:

	==Crystal structure of Ct1,3Gal43A in complex with galactan==		==Crystal structure of Ct1,3Gal43A in complex with galactan==
	<StructureSection load='3vsz' size='340' side='right' caption='[[3vsz]], [[Resolution\|resolution]] 2.89Å' scene=''>		<StructureSection load='3vsz' size='340' side='right'caption='[[3vsz]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3vsz]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cloth Cloth~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VSZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3vsz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Acet2 Acet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VSZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vsf\|3vsf]], [[3vt0\|3vt0]], [[3vt1\|3vt1]], [[3vt2\|3vt2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vsf\|3vsf]], [[3vt0\|3vt0]], [[3vt1\|3vt1]], [[3vt2\|3vt2]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cthe_0661 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 ~~CLOTH~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cthe_0661 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 ACET2])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vsz OCA], [~~http~~://pdbe.org/3vsz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vsz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vsz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vsz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vsz OCA], [https://pdbe.org/3vsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vsz RCSB], [https://www.ebi.ac.uk/pdbsum/3vsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vsz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
	*[[Ricin\|Ricin]]		*[[Ricin 3D structures\|Ricin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Cloth~~]]		[[Category: Acet2]]
			[[Category: Large Structures]]
	[[Category: Fan, J]]		[[Category: Fan, J]]
	[[Category: Huang, B]]		[[Category: Huang, B]]

OCA at 13:44, 11 August 2016

2016-08-11T13:44:58Z

← Older revision		Revision as of 13:44, 11 August 2016
Line 1:		Line 1:

	==Crystal structure of Ct1,3Gal43A in complex with galactan==		==Crystal structure of Ct1,3Gal43A in complex with galactan==
	<StructureSection load='3vsz' size='340' side='right' caption='[[3vsz]], [[Resolution\|resolution]] 2.89Å' scene=''>		<StructureSection load='3vsz' size='340' side='right' caption='[[3vsz]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3vsz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_thermocellum_atcc_27405 Clostridium thermocellum atcc 27405~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VSZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3vsz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Cloth Cloth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VSZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vsf\|3vsf]], [[3vt0\|3vt0]], [[3vt1\|3vt1]], [[3vt2\|3vt2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vsf\|3vsf]], [[3vt0\|3vt0]], [[3vt1\|3vt1]], [[3vt2\|3vt2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cthe_0661 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 ~~Clostridium thermocellum ATCC 27405~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cthe_0661 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 CLOTH])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vsz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vsz RCSB], [http://www.ebi.ac.uk/pdbsum/3vsz PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vsz OCA], [http://pdbe.org/3vsz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vsz RCSB], [http://www.ebi.ac.uk/pdbsum/3vsz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vsz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3vsz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Clostridium thermocellum atcc 27405~~]]		[[Category: Cloth]]
	[[Category: Fan, J]]		[[Category: Fan, J]]
	[[Category: Huang, B]]		[[Category: Huang, B]]

OCA at 10:45, 4 January 2015

2015-01-04T10:45:26Z

← Older revision		Revision as of 10:45, 4 January 2015
Line 1:		Line 1:
	[[~~Image~~:~~3vsz~~.~~jpg~~\|~~left~~\|~~200px~~]]		==Crystal structure of Ct1,3Gal43A in complex with galactan==
			<StructureSection load='3vsz' size='340' side='right' caption='[[3vsz]], [[Resolution\|resolution]] 2.89Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3vsz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum_atcc_27405 Clostridium thermocellum atcc 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VSZ FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vsf\|3vsf]], [[3vt0\|3vt0]], [[3vt1\|3vt1]], [[3vt2\|3vt2]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cthe_0661 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 Clostridium thermocellum ATCC 27405])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vsz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vsz RCSB], [http://www.ebi.ac.uk/pdbsum/3vsz PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Glycoside hydrolase family 43 (GH43) consists of a variety of enzymes distributed widely in prokaryotes and eukaryotes. The mechanism by which GH43 enzymes hydrolyze oligosaccharides requires three essential acidic amino acid residues. However, one of them is thought to be missing in galactan beta-1,3-galactosidases from the GH43 family. Ct1,3Gal43A, from Clostridium thermocellum, is comprised of a GH43 domain, a CBM13 domain, and a dockerin domain and exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch. Here, we present its crystal structure at 2.7 A resolution and complex structures of the enzyme with several substrates and analogs. Two modes of substrate binding were observed at the beta site of the CtCBM13 domain, and one galactobiose molecule was found in an "L" shaped pocket of the CtGH43 domain, which appears large enough to accommodate two more galactose units. In addition, we found that mutating Glu112 to Gln or Ala eliminated the galactan hydrolysis activity of Ct1,3Gal43A while did not disrupt its ligand binding ability. Combining this results and the crystal structure we identified Glu112 in Ct1,3Gal43A as the 'missing' essential acidic residue in galactan beta-1,3-galactosidases. Structural information presented here also suggests a mechanism by which Ct1,3Gal43A bypasses beta-1,6 linked branches in the substrate and another mechanism by which the substrate is delivered 'in trans' from the CBM13 domain to the catalytic GH43 domain.

	~~{{STRUCTURE_3vsz\| PDB=3vsz \| SCENE= }}~~		Crystal structure of 1,3Gal43A, an exo-beta-1,3-galactanase from Clostridium thermocellum.,Jiang D, Fan J, Wang X, Zhao Y, Huang B, Liu J, Zhang XC J Struct Biol. 2012 Dec;180(3):447-57. doi: 10.1016/j.jsb.2012.08.005. Epub 2012 , Aug 29. PMID:22960181<ref>PMID:22960181</ref>

	~~===Crystal structure~~ of ~~Ct1,3Gal43A in complex with galactan===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22960181}}~~		==See Also==
			*[[Ricin\|Ricin]]
	==~~About this Structure~~==		== References ==
	[[~~3vsz~~]] ~~is a 6 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Clostridium_thermocellum_atcc_27405 Clostridium thermocellum atcc 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA].		<references/>
			__TOC__
			</StructureSection>
	[[Category: Clostridium thermocellum atcc 27405]]		[[Category: Clostridium thermocellum atcc 27405]]
	[[Category: Fan, J.]]		[[Category: Fan, J]]
	[[Category: Huang, B.]]		[[Category: Huang, B]]
	[[Category: Jiang, D.]]		[[Category: Jiang, D]]
	[[Category: Wang, X.]]		[[Category: Wang, X]]
	[[Category: Zhang, X C.]]		[[Category: Zhang, X C]]
	[[Category: Zhao, Y.]]		[[Category: Zhao, Y]]
	[[Category: Cbm13]]		[[Category: Cbm13]]
	[[Category: Galactan hydrolysis]]		[[Category: Galactan hydrolysis]]
	[[Category: Gh43]]		[[Category: Gh43]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

OCA at 10:50, 5 December 2012

2012-12-05T10:50:36Z

← Older revision		Revision as of 10:50, 5 December 2012
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:3vsz.jpg\|left\|200px]]

	~~The entry~~ 3vsz ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_3vsz\| PDB=3vsz \| SCENE= }}

	~~Authors: Jiang~~, ~~D., Fan, J., Wang, X., Zhao, Y., Huang, B., Zhang, X.C.~~		===Crystal structure of Ct1,3Gal43A in complex with galactan===

	~~Description~~: ~~Crystal structure of Ct1~~,~~3Gal43A in complex with galactan~~		{{ABSTRACT_PUBMED_22960181}}

			==About this Structure==
			[[3vsz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum_atcc_27405 Clostridium thermocellum atcc 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA].
			[[Category: Clostridium thermocellum atcc 27405]]
			[[Category: Fan, J.]]
			[[Category: Huang, B.]]
			[[Category: Jiang, D.]]
			[[Category: Wang, X.]]
			[[Category: Zhang, X C.]]
			[[Category: Zhao, Y.]]
			[[Category: Cbm13]]
			[[Category: Galactan hydrolysis]]
			[[Category: Gh43]]
			[[Category: Sugar binding protein]]

OCA at 05:19, 22 August 2012

2012-08-22T05:19:01Z

← Older revision		Revision as of 05:19, 22 August 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3vsz is ON HOLD		The entry 3vsz is ON HOLD until Paper Publication

	Authors: Jiang, D., Fan, J., Wang, X., Zhao, Y., Huang, B., Zhang, X.C.		Authors: Jiang, D., Fan, J., Wang, X., Zhao, Y., Huang, B., Zhang, X.C.

	Description: Crystal structure of Ct1,3Gal43A in complex with galactan		Description: Crystal structure of Ct1,3Gal43A in complex with galactan

OCA at 07:09, 6 June 2012

2012-06-06T07:09:08Z

← Older revision		Revision as of 07:09, 6 June 2012
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	The entry 3vsz is ON HOLD		The entry 3vsz is ON HOLD

	Authors: Jiang, ~~Dao-hua~~, Fan, ~~Jun-ping~~, Wang, ~~Xian-ping~~, Zhao, ~~Yan~~, Huang, Bo, Zhang, X. ~~Cai~~		Authors: Jiang, D., Fan, J., Wang, X., Zhao, Y., Huang, B., Zhang, X.C.

	Description: Crystal structure of Ct1,3Gal43A in complex with galactan		Description: Crystal structure of Ct1,3Gal43A in complex with galactan

OCA: Protected "3vsz" [edit=sysop:move=sysop]

2012-05-23T05:30:05Z

Protected "3vsz" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:30, 23 May 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 3vsz is ON HOLD Authors: Jiang, Dao-hua, Fan, Jun-ping, Wang, Xian-ping, Zhao, Yan, Huang, Bo, Zhang, X. Cai Description: Crystal structure of Ct1,...

2012-05-23T05:30:04Z

New page: '''Unreleased structure''' The entry 3vsz is ON HOLD Authors: Jiang, Dao-hua, Fan, Jun-ping, Wang, Xian-ping, Zhao, Yan, Huang, Bo, Zhang, X. Cai Description: Crystal structure of Ct1,...

New page

'''Unreleased structure'''

The entry 3vsz is ON HOLD

Authors: Jiang, Dao-hua, Fan, Jun-ping, Wang, Xian-ping, Zhao, Yan, Huang, Bo, Zhang, X. Cai

Description: Crystal structure of Ct1,3Gal43A in complex with galactan