3zzi - Revision history

OCA at 11:16, 20 December 2023

2023-12-20T11:16:38Z

← Older revision		Revision as of 11:16, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='3zzi' size='340' side='right'caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='3zzi' size='340' side='right'caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[3zzh\|3zzh]], [[3zzf\|3zzf]], [[3zzg\|3zzg]]~~, [[~~4ab7~~\|~~4ab7~~]]~~</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.8Å</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 ~~2.7.2.8] </span>~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [https://pdbe.org/3zzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [https://www.ebi.ac.uk/pdbsum/3zzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [https://pdbe.org/3zzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [https://www.ebi.ac.uk/pdbsum/3zzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzi ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/ARG56_YEAST ARG56_YEAST]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Acetylglutamate kinase]]~~
	~~[[Category: Atcc 18824]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Cima, S de~~]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Crabeel, M]]		[[Category: Crabeel M]]
	[[Category: Fita, I]]		[[Category: Fita I]]
	[[Category: Gil-Ortiz, F]]		[[Category: Gil-Ortiz F]]
	[[Category: Rubio, V]]		[[Category: Rubio V]]
	[[Category: ~~Arginine biosynthesis]]~~		[[Category: De Cima S]]
	~~[[Category: Transferase~~]]

OCA at 07:30, 18 August 2022

2022-08-18T07:30:17Z

← Older revision		Revision as of 07:30, 18 August 2022
Line 3:		Line 3:
	<StructureSection load='3zzi' size='340' side='right'caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='3zzi' size='340' side='right'caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zzh\|3zzh]], [[3zzf\|3zzf]], [[3zzg\|3zzg]], [[4ab7\|4ab7]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zzh\|3zzh]], [[3zzf\|3zzf]], [[3zzg\|3zzg]], [[4ab7\|4ab7]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [~~http~~://pdbe.org/3zzi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zzi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [https://pdbe.org/3zzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [https://www.ebi.ac.uk/pdbsum/3zzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:33, 10 May 2019

2019-05-10T12:33:34Z

← Older revision		Revision as of 12:33, 10 May 2019
Line 1:		Line 1:

	==Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae==		==Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae==
	<StructureSection load='3zzi' size='340' side='right' caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='3zzi' size='340' side='right'caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>
Line 23:		Line 23:
	[[Category: Acetylglutamate kinase]]		[[Category: Acetylglutamate kinase]]
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
			[[Category: Large Structures]]
	[[Category: Cima, S de]]		[[Category: Cima, S de]]
	[[Category: Crabeel, M]]		[[Category: Crabeel, M]]

OCA at 16:30, 27 February 2019

2019-02-27T16:30:09Z

← Older revision		Revision as of 16:30, 27 February 2019
Line 23:		Line 23:
	[[Category: Acetylglutamate kinase]]		[[Category: Acetylglutamate kinase]]
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
	[[Category: Cima, S De]]		[[Category: Cima, S de]]
	[[Category: Crabeel, M]]		[[Category: Crabeel, M]]
	[[Category: Fita, I]]		[[Category: Fita, I]]

OCA at 13:29, 11 August 2016

2016-08-11T13:29:45Z

← Older revision		Revision as of 13:29, 11 August 2016
Line 1:		Line 1:

	==Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae==		==Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae==
	<StructureSection load='3zzi' size='340' side='right' caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='3zzi' size='340' side='right' caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zzh\|3zzh]], [[3zzf\|3zzf]], [[3zzg\|3zzg]], [[4ab7\|4ab7]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zzh\|3zzh]], [[3zzf\|3zzf]], [[3zzg\|3zzg]], [[4ab7\|4ab7]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [http://www.ebi.ac.uk/pdbsum/3zzi PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [http://pdbe.org/3zzi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [http://www.ebi.ac.uk/pdbsum/3zzi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3zzi" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Acetylglutamate kinase]]		[[Category: Acetylglutamate kinase]]
	[[Category: ~~Saccharomyces cerevisiae~~]]		[[Category: Atcc 18824]]
	[[Category: Cima, S De]]		[[Category: Cima, S De]]
	[[Category: Crabeel, M]]		[[Category: Crabeel, M]]

OCA at 10:28, 4 January 2015

2015-01-04T10:28:56Z

← Older revision		Revision as of 10:28, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zzh\|3zzh]], [[3zzf\|3zzf]], [[3zzg\|3zzg]], [[4ab7\|4ab7]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zzh\|3zzh]], [[3zzf\|3zzf]], [[3zzg\|3zzg]], [[4ab7\|4ab7]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [http://www.ebi.ac.uk/pdbsum/3zzi PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [http://www.ebi.ac.uk/pdbsum/3zzi PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Insight on an arginine synthesis metabolon from the tetrameric structure of yeast acetylglutamate kinase.,de Cima S, Gil-Ortiz F, Crabeel M, Fita I, Rubio V PLoS One. 2012;7(4):e34734. Epub 2012 Apr 18. PMID:22529931<ref>PMID:22529931</ref>		Insight on an arginine synthesis metabolon from the tetrameric structure of yeast acetylglutamate kinase.,de Cima S, Gil-Ortiz F, Crabeel M, Fita I, Rubio V PLoS One. 2012;7(4):e34734. Epub 2012 Apr 18. PMID:22529931<ref>PMID:22529931</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	[[Category: Acetylglutamate kinase]]		[[Category: Acetylglutamate kinase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Cima, S De.]]		[[Category: Cima, S De]]
	[[Category: Crabeel, M.]]		[[Category: Crabeel, M]]
	[[Category: Fita, I.]]		[[Category: Fita, I]]
	[[Category: Gil-Ortiz, F.]]		[[Category: Gil-Ortiz, F]]
	[[Category: Rubio, V.]]		[[Category: Rubio, V]]
	[[Category: Arginine biosynthesis]]		[[Category: Arginine biosynthesis]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 07:27, 5 June 2014

2014-06-05T07:27:44Z

← Older revision		Revision as of 07:27, 5 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~3zzi~~.~~jpg~~\|~~left~~\|~~200px~~]]		==Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae==
			<StructureSection load='3zzi' size='340' side='right' caption='[[3zzi]], [[Resolution\|resolution]] 3.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZI FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zzh\|3zzh]], [[3zzf\|3zzf]], [[3zzg\|3zzg]], [[4ab7\|4ab7]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzi RCSB], [http://www.ebi.ac.uk/pdbsum/3zzi PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			N-acetyl-L-glutamate kinase (NAGK) catalyzes the second, generally controlling, step of arginine biosynthesis. In yeasts, NAGK exists either alone or forming a metabolon with N-acetyl-L-glutamate synthase (NAGS), which catalyzes the first step and exists only within the metabolon. Yeast NAGK (yNAGK) has, in addition to the amino acid kinase (AAK) domain found in other NAGKs, a approximately 150-residue C-terminal domain of unclear significance belonging to the DUF619 domain family. We deleted this domain, proving that it stabilizes yNAGK, slows catalysis and modulates feed-back inhibition by arginine. We determined the crystal structures of both the DUF619 domain-lacking yNAGK, ligand-free as well as complexed with acetylglutamate or acetylglutamate and arginine, and of complete mature yNAGK. While all other known arginine-inhibitable NAGKs are doughnut-like hexameric trimers of dimers of AAK domains, yNAGK has as central structure a flat tetramer formed by two dimers of AAK domains. These dimers differ from canonical AAK dimers in the -110 degrees rotation of one subunit with respect to the other. In the hexameric enzymes, an N-terminal extension, found in all arginine-inhibitable NAGKs, forms a protruding helix that interlaces the dimers. In yNAGK, however, it conforms a two-helix platform that mediates interdimeric interactions. Arginine appears to freeze an open inactive AAK domain conformation. In the complete yNAGK structure, two pairs of DUF619 domains flank the AAK domain tetramer, providing a mechanism for the DUF619 domain modulatory functions. The DUF619 domain exhibits the histone acetyltransferase fold, resembling the catalytic domain of bacterial NAGS. However, the putative acetyl CoA site is blocked, explaining the lack of NAGS activity of yNAGK. We conclude that the tetrameric architecture is an adaptation to metabolon formation and propose an organization for this metabolon, suggesting that yNAGK may be a good model also for yeast and human NAGSs.

	<!--		Insight on an arginine synthesis metabolon from the tetrameric structure of yeast acetylglutamate kinase.,de Cima S, Gil-Ortiz F, Crabeel M, Fita I, Rubio V PLoS One. 2012;7(4):e34734. Epub 2012 Apr 18. PMID:22529931<ref>PMID:22529931</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3zzi"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter~~ (~~which sets the initial scene displayed when the page is loaded~~),
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3zzi\| PDB=3zzi \| SCENE= }}~~

	~~===Crystal structure~~ of ~~a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	~~<!--~~		<references/>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22529931}}, adds the Publication Abstract to~~ the ~~page~~		__TOC__
	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22529931 is the PubMed ID number~~.		</StructureSection>
	-->
	~~{{ABSTRACT_PUBMED_22529931}}~~

	~~==About this Structure==~~
	~~[[3zzi]] is a 8 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA].~~

	==~~Reference~~==
	<~~ref group="xtra">PMID:022529931<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Acetylglutamate kinase]]		[[Category: Acetylglutamate kinase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]

OCA at 08:10, 9 May 2012

2012-05-09T08:10:16Z

← Older revision		Revision as of 08:10, 9 May 2012
Line 11:		Line 11:
	===Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae===		===Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22529931}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22529931 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22529931}}

	==About this Structure==		==About this Structure==
	[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA].		[[3zzi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZI OCA].

			==Reference==
			<ref group="xtra">PMID:022529931</ref><references group="xtra"/>
	[[Category: Acetylglutamate kinase]]		[[Category: Acetylglutamate kinase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]

OCA at 19:27, 2 May 2012

2012-05-02T19:27:07Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3zzi.jpg|left|200px]]
-The entry 3zzi is ON HOLD  until Paper Publication
+<!--
-Authors: de Cima, S., Gil-Ortiz, F., Crabeel, M., Fita, I., Rubio, V.
+===Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae===
-Description: Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae

OCA at 05:21, 28 September 2011

2011-09-28T05:21:46Z

← Older revision		Revision as of 05:21, 28 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3zzi is ON HOLD		The entry 3zzi is ON HOLD until Paper Publication

	Authors: de Cima, S., Gil-Ortiz, F., Crabeel, M., Fita, I., Rubio, V.		Authors: de Cima, S., Gil-Ortiz, F., Crabeel, M., Fita, I., Rubio, V.

	Description: Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae		Description: Crystal structure of a tetrameric acetylglutamate kinase from Saccharomyces cerevisiae