4aef - Revision history

OCA at 11:25, 20 December 2023

2023-12-20T11:25:07Z

← Older revision		Revision as of 11:25, 20 December 2023
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	<StructureSection load='4aef' size='340' side='right'caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>		<StructureSection load='4aef' size='340' side='right'caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43587 Atcc 43587~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AEF FirstGlance]. <br>
	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Neopullulanase Neopullulanase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.~~2.~~1.135] </span>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.34Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aef OCA], [https://pdbe.org/4aef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aef RCSB], [https://www.ebi.ac.uk/pdbsum/4aef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aef OCA], [https://pdbe.org/4aef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aef RCSB], [https://www.ebi.ac.uk/pdbsum/4aef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aef ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q8TZP8_PYRFU Q8TZP8_PYRFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 43587]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Neopullulanase~~]]		[[Category: Pyrococcus furiosus]]
	[[Category: Jung, T Y]]		[[Category: Jung T-Y]]
	[[Category: Park, K H]]		[[Category: Park K-H]]
	[[Category: Song, H N]]		[[Category: Song H-N]]
	[[Category: Woo, E J]]		[[Category: Woo E-J]]
	[[Category: Yang, S J]]		[[Category: Yang S-J]]
	[[Category: Yoon, S M]]		[[Category: Yoon S-M]]
	~~[[Category: High temperature]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Thermostability~~]]

OCA at 05:31, 25 August 2022

2022-08-25T05:31:29Z

← Older revision		Revision as of 05:31, 25 August 2022
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	<StructureSection load='4aef' size='340' side='right'caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>		<StructureSection load='4aef' size='340' side='right'caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4AEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AEF FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aef OCA], [~~http~~://pdbe.org/4aef PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4aef RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4aef PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4aef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aef OCA], [https://pdbe.org/4aef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aef RCSB], [https://www.ebi.ac.uk/pdbsum/4aef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aef ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Amylase\|Amylase]]		*[[Amylase 3D structures\|Amylase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 43587]]		[[Category: Atcc 43587]]
			[[Category: Large Structures]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
	[[Category: Jung, T Y]]		[[Category: Jung, T Y]]

OCA at 09:43, 6 March 2019

2019-03-06T09:43:13Z

← Older revision		Revision as of 09:43, 6 March 2019
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	==THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS==		==THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS==
	<StructureSection load='4aef' size='340' side='right' caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>		<StructureSection load='4aef' size='340' side='right'caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AEF FirstGlance]. <br>
Line 19:		Line 19:
	==See Also==		==See Also==
	*[[Amylase\|Amylase]]		*[[Amylase\|Amylase]]
	*[[User:Gabriel Pons/Sandbox 2\|User:Gabriel Pons/Sandbox 2]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:12, 10 December 2016

2016-12-10T19:12:17Z

← Older revision		Revision as of 19:12, 10 December 2016
Line 1:		Line 1:

	==THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS==		==THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS==
	<StructureSection load='4aef' size='340' side='right' caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>		<StructureSection load='4aef' size='340' side='right' caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_furiosus Pyrococcus furiosus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AEF FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aef RCSB], [http://www.ebi.ac.uk/pdbsum/4aef PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aef OCA], [http://pdbe.org/4aef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4aef RCSB], [http://www.ebi.ac.uk/pdbsum/4aef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4aef ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4aef" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 43587]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
	~~[[Category: Pyrococcus furiosus]]~~
	[[Category: Jung, T Y]]		[[Category: Jung, T Y]]
	[[Category: Park, K H]]		[[Category: Park, K H]]

OCA at 17:53, 9 December 2014

2014-12-09T17:53:25Z

← Older revision		Revision as of 17:53, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:4aef.~~png\|left\|200px~~]]		==THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS==
			<StructureSection load='4aef' size='340' side='right' caption='[[4aef]], [[Resolution\|resolution]] 2.34Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AEF FirstGlance]. <br>
			</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aef RCSB], [http://www.ebi.ac.uk/pdbsum/4aef PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			PFTA (Pyrococcus furiosus thermostable amylase) is a hyperthermophilic amylase isolated from the archaeon Pyrococcus furiosus. This enzyme possesses characteristics of both alpha-amylase- and cyclodextrin (CD)-hydrolyzing enzymes, allowing it to degrade pullulan, CD and acarbose-activities that are absent in most alpha-amylases-without the transferring activity that is common in CD-hydrolyzing enzymes. The crystal structure of PFTA revealed a unique monomeric subunit with an extended N-terminal region and an N'-domain folded into its own active site-a significantly altered domain configuration relative to that of the conventional dimeric CD-hydrolyzing amylases in glycoside hydrolase family 13. The active site is formed by the interface of the N'-domain and the catalytic domain and exhibits a broad and wide-open geometry without the concave pocket that is commonly found in the active sites of maltogenic amylases. The mutation of a residue (Gly415 to Glu) located at the domain interface between the N'- and catalytic domains yielded an enzyme that produced a significantly higher purity maltoheptaose (G7) from beta-CD, supporting the involvement of this interface in substrate recognition and indicating that this mutant enzyme is a suitable candidate for the production of pure G7. The unique configuration of the active site distinguishes this archaic monomeric enzyme from classical bacterial CD-hydrolyzing amylases and provides a molecular basis for its enzymatic characteristics and for its potential use in industrial applications.

	~~{{STRUCTURE_4aef\| PDB=4aef \| SCENE= }}~~		A novel domain arrangement in a monomeric cyclodextrin-hydrolyzing enzyme from the hyperthermophile Pyrococcus furiosus.,Park JT, Song HN, Jung TY, Lee MH, Park SG, Woo EJ, Park KH Biochim Biophys Acta. 2013 Jan;1834(1):380-6. doi: 10.1016/j.bbapap.2012.08.001. , Epub 2012 Aug 8. PMID:22902546<ref>PMID:22902546</ref>

	~~===THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22902546}}~~

	~~==About this Structure==~~
	~~[[4aef]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=4AEF OCA].~~

	==See Also==		==See Also==
	*[[~~Alpha-~~Amylase\|~~Alpha-~~Amylase]]		*[[Amylase\|Amylase]]
			*[[User:Gabriel Pons/Sandbox 2\|User:Gabriel Pons/Sandbox 2]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
	[[Category: Pyrococcus furiosus]]		[[Category: Pyrococcus furiosus]]
	[[Category: Jung, T Y.]]		[[Category: Jung, T Y]]
	[[Category: Park, K H.]]		[[Category: Park, K H]]
	[[Category: Song, H N.]]		[[Category: Song, H N]]
	[[Category: Woo, E J.]]		[[Category: Woo, E J]]
	[[Category: Yang, S J.]]		[[Category: Yang, S J]]
	[[Category: Yoon, S M.]]		[[Category: Yoon, S M]]
	[[Category: High temperature]]		[[Category: High temperature]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Thermostability]]		[[Category: Thermostability]]

OCA at 10:51, 23 January 2013

2013-01-23T10:51:04Z

← Older revision		Revision as of 10:51, 23 January 2013
Line 9:		Line 9:
	==About this Structure==		==About this Structure==
	[[4aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA].		[[4aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA].

			==See Also==
			*[[Alpha-Amylase\|Alpha-Amylase]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
	[[Category: Pyrococcus furiosus]]		[[Category: Pyrococcus furiosus]]

OCA at 09:19, 16 January 2013

2013-01-16T09:19:19Z

← Older revision		Revision as of 09:19, 16 January 2013
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	[[Image:4aef.~~jpg~~\|left\|200px]]		[[Image:4aef.png\|left\|200px]]

	{{STRUCTURE_4aef\| PDB=4aef \| SCENE= }}		{{STRUCTURE_4aef\| PDB=4aef \| SCENE= }}

OCA at 09:10, 31 October 2012

2012-10-31T09:10:53Z

← Older revision		Revision as of 09:10, 31 October 2012
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4aef.jpg\|left\|200px]]

	~~The entry~~ 4aef ~~is ON HOLD~~		{{STRUCTURE_4aef\| PDB=4aef \| SCENE= }}

	~~Authors: SONG, H.-N., JUNG, T.-Y., YOON, S.-M., YANG, S.-J., PARK, K.-H., WOO, E.-J.~~		===THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS===

	~~Description~~: ~~THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS~~		{{ABSTRACT_PUBMED_22902546}}

			==About this Structure==
			[[4aef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AEF OCA].
			[[Category: Neopullulanase]]
			[[Category: Pyrococcus furiosus]]
			[[Category: Jung, T Y.]]
			[[Category: Park, K H.]]
			[[Category: Song, H N.]]
			[[Category: Woo, E J.]]
			[[Category: Yang, S J.]]
			[[Category: Yoon, S M.]]
			[[Category: High temperature]]
			[[Category: Hydrolase]]
			[[Category: Thermostability]]

OCA: Protected "4aef" [edit=sysop:move=sysop]

2012-01-18T10:25:36Z

Protected "4aef" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:25, 18 January 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4aef is ON HOLD Authors: SONG, H.-N., JUNG, T.-Y., YOON, S.-M., YANG, S.-J., PARK, K.-H., WOO, E.-J. Description: THE CRYSTAL STRUCTURE OF THERMOST...

2012-01-18T10:25:35Z

New page: '''Unreleased structure''' The entry 4aef is ON HOLD Authors: SONG, H.-N., JUNG, T.-Y., YOON, S.-M., YANG, S.-J., PARK, K.-H., WOO, E.-J. Description: THE CRYSTAL STRUCTURE OF THERMOST...

New page

'''Unreleased structure'''

The entry 4aef is ON HOLD

Authors: SONG, H.-N., JUNG, T.-Y., YOON, S.-M., YANG, S.-J., PARK, K.-H., WOO, E.-J.

Description: THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS