4b8a - Revision history

OCA at 11:45, 20 December 2023

2023-12-20T11:45:38Z

← Older revision		Revision as of 11:45, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4b8a' size='340' side='right'caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4b8a' size='340' side='right'caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B8A FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]]~~, [[~~4b8c~~\|~~4b8c~~]]~~</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 ~~3.1.13.4] </span>~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [https://pdbe.org/4b8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [https://www.ebi.ac.uk/pdbsum/4b8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b8a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [https://pdbe.org/4b8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [https://www.ebi.ac.uk/pdbsum/4b8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/NOT1_YEAST NOT1_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref> [[https://www.uniprot.org/uniprot/POP2_YEAST POP2_YEAST]] Acts as probably catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. In vitro, POP2 has 3'-exoribonuclease activity with a preference for poly(A) RNAs, but also degrades poly(U) and poly(C) RNAs. Is part of a glucose-sensing system involved in growth control in response to glucose availability.<ref>PMID:9463387</ref> <ref>PMID:11239395</ref> <ref>PMID:11410650</ref> <ref>PMID:14618157</ref>		[https://www.uniprot.org/uniprot/NOT1_YEAST NOT1_YEAST] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Baker's yeast]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Basquin, J~~]]		[[Category: Saccharomyces cerevisiae S288C]]
	[[Category: ~~Conti, E~~]]		[[Category: Basquin J]]
	[[Category: ~~Transcription-hydrolase complex~~]]		[[Category: Conti E]]

OCA at 07:03, 31 August 2022

2022-08-31T07:03:27Z

← Older revision		Revision as of 07:03, 31 August 2022
Line 3:		Line 3:
	<StructureSection load='4b8a' size='340' side='right'caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4b8a' size='340' side='right'caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4B8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B8A FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [~~http~~://pdbe.org/4b8a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4b8a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4b8a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [https://pdbe.org/4b8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [https://www.ebi.ac.uk/pdbsum/4b8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/NOT1_YEAST NOT1_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref> [[~~http~~://www.uniprot.org/uniprot/POP2_YEAST POP2_YEAST]] Acts as probably catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. In vitro, POP2 has 3'-exoribonuclease activity with a preference for poly(A) RNAs, but also degrades poly(U) and poly(C) RNAs. Is part of a glucose-sensing system involved in growth control in response to glucose availability.<ref>PMID:9463387</ref> <ref>PMID:11239395</ref> <ref>PMID:11410650</ref> <ref>PMID:14618157</ref>		[[https://www.uniprot.org/uniprot/NOT1_YEAST NOT1_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref> [[https://www.uniprot.org/uniprot/POP2_YEAST POP2_YEAST]] Acts as probably catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. In vitro, POP2 has 3'-exoribonuclease activity with a preference for poly(A) RNAs, but also degrades poly(U) and poly(C) RNAs. Is part of a glucose-sensing system involved in growth control in response to glucose availability.<ref>PMID:9463387</ref> <ref>PMID:11239395</ref> <ref>PMID:11410650</ref> <ref>PMID:14618157</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	*[[Temp\|Temp]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:16, 28 August 2019

2019-08-28T16:16:05Z

← Older revision		Revision as of 16:16, 28 August 2019
Line 1:		Line 1:

	==Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1==		==Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1==
	<StructureSection load='4b8a' size='340' side='right' caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4b8a' size='340' side='right'caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B8A FirstGlance]. <br>
Line 21:		Line 21:

	==See Also==		==See Also==
			*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==
Line 27:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
			[[Category: Large Structures]]
	[[Category: Basquin, J]]		[[Category: Basquin, J]]
	[[Category: Conti, E]]		[[Category: Conti, E]]
	[[Category: Transcription-hydrolase complex]]		[[Category: Transcription-hydrolase complex]]

OCA at 12:55, 16 November 2017

2017-11-16T12:55:11Z

← Older revision		Revision as of 12:55, 16 November 2017
Line 6:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [http://pdbe.org/4b8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [http://www.ebi.ac.uk/pdbsum/4b8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [http://pdbe.org/4b8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [http://www.ebi.ac.uk/pdbsum/4b8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 21:

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==

OCA at 15:53, 10 May 2016

2016-05-10T15:53:05Z

← Older revision		Revision as of 15:53, 10 May 2016
Line 1:		Line 1:

	==Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1==		==Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1==
	<StructureSection load='4b8a' size='340' side='right' caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4b8a' size='340' side='right' caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [http://www.ebi.ac.uk/pdbsum/4b8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [http://pdbe.org/4b8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [http://www.ebi.ac.uk/pdbsum/4b8a PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4b8a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:13, 25 December 2014

2014-12-25T12:13:04Z

← Older revision		Revision as of 12:13, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B8A FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [http://www.ebi.ac.uk/pdbsum/4b8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [http://www.ebi.ac.uk/pdbsum/4b8a PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/NOT1_YEAST NOT1_YEAST]] Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.<ref>PMID:9463387</ref> [[http://www.uniprot.org/uniprot/POP2_YEAST POP2_YEAST]] Acts as probably catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. In vitro, POP2 has 3'-exoribonuclease activity with a preference for poly(A) RNAs, but also degrades poly(U) and poly(C) RNAs. Is part of a glucose-sensing system involved in growth control in response to glucose availability.<ref>PMID:9463387</ref> <ref>PMID:11239395</ref> <ref>PMID:11410650</ref> <ref>PMID:14618157</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
			*[[Temp\|Temp]]
			*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
	[[Category: Basquin, J.]]		[[Category: Basquin, J]]
	[[Category: Conti, E.]]		[[Category: Conti, E]]
	[[Category: Transcription-hydrolase complex]]		[[Category: Transcription-hydrolase complex]]

OCA at 06:21, 13 August 2014

2014-08-13T06:21:55Z

← Older revision		Revision as of 06:21, 13 August 2014
Line 1:		Line 1:
	[[~~Image~~:~~4b8a~~.~~jpg~~\|~~left~~\|~~200px~~]]		==Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1==
			<StructureSection load='4b8a' size='340' side='right' caption='[[4b8a]], [[Resolution\|resolution]] 2.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4b8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B8A FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uoc\|1uoc]], [[4b89\|4b89]], [[4b8b\|4b8b]], [[4b8c\|4b8c]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b8a RCSB], [http://www.ebi.ac.uk/pdbsum/4b8a PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Shortening eukaryotic poly(A) tails represses mRNA translation and induces mRNA turnover. The major cytoplasmic deadenylase, the Ccr4-Not complex, is a conserved multisubunit assembly. Ccr4-Not is organized around Not1, a large scaffold protein that recruits two 3'-5' exoribonucleases, Caf1 and Ccr4. We report structural studies showing that the N-terminal arm of yeast Not1 has a HEAT-repeat structure with domains related to the MIF4G fold. A MIF4G domain positioned centrally within the Not1 protein recognizes Caf1, which in turn binds the LRR domain of Ccr4 and tethers the Ccr4 nuclease domain. The interactions that form the nuclease core of the Ccr4-Not complex are evolutionarily conserved. Their specific disruption affects cell growth and mRNA deadenylation and decay in vivo in yeast. Thus, the N-terminal arm of Not1 forms an extended platform reminiscent of scaffolding proteins like eIF4G and CBP80, and places the two nucleases in a pivotal position within the Ccr4-Not complex.

	~~{{STRUCTURE_4b8a\| PDB=4b8a \| SCENE= }}~~		Architecture of the nuclease module of the yeast ccr4-not complex: the not1-caf1-ccr4 interaction.,Basquin J, Roudko VV, Rode M, Basquin C, Seraphin B, Conti E Mol Cell. 2012 Oct 26;48(2):207-18. doi: 10.1016/j.molcel.2012.08.014. Epub 2012 , Sep 6. PMID:22959269<ref>PMID:22959269</ref>

	~~===Structure~~ of ~~yeast NOT1 MIF4G domain co-crystallized with CAF1===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22959269}}~~		==See Also==
			*[[Ribonuclease\|Ribonuclease]]
	==~~About this Structure~~==		== References ==
	[[~~4b8a~~]] ~~is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA].~~		<references/>
	[[Category: ~~Saccharomyces cerevisiae s288c~~]]		__TOC__
			</StructureSection>
			[[Category: Baker's yeast]]
	[[Category: Basquin, J.]]		[[Category: Basquin, J.]]
	[[Category: Conti, E.]]		[[Category: Conti, E.]]
	[[Category: Transcription-hydrolase complex]]		[[Category: Transcription-hydrolase complex]]

OCA at 09:27, 21 November 2012

2012-11-21T09:27:32Z

← Older revision		Revision as of 09:27, 21 November 2012
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4b8a.jpg\|left\|200px]]

	~~The entry~~ 4b8a ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_4b8a\| PDB=4b8a \| SCENE= }}

	~~Authors: Basquin, J., Conti, E.~~		===Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1===

	~~Description~~: ~~Structure of yeast NOT1 MIF4G domain co~~-~~crystallized with CAF1~~		{{ABSTRACT_PUBMED_22959269}}

			==About this Structure==
			[[4b8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8A OCA].
			[[Category: Saccharomyces cerevisiae s288c]]
			[[Category: Basquin, J.]]
			[[Category: Conti, E.]]
			[[Category: Transcription-hydrolase complex]]

OCA at 06:43, 19 September 2012

2012-09-19T06:43:52Z

← Older revision		Revision as of 06:43, 19 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4b8a is ON HOLD		The entry 4b8a is ON HOLD until Paper Publication

	Authors: Basquin, J., Conti, E.		Authors: Basquin, J., Conti, E.

	Description: Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1		Description: Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1

OCA: Protected "4b8a" [edit=sysop:move=sysop]

2012-09-05T04:44:23Z

Protected "4b8a" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:44, 5 September 2012
(No difference)