4blm - Revision history

OCA at 10:40, 1 March 2024

2024-03-01T10:40:29Z

← Older revision		Revision as of 10:40, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='4blm' size='340' side='right'caption='[[4blm]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4blm' size='340' side='right'caption='[[4blm]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4blm]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898~~]. The May 2002 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BLM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4blm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BLM FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]~~ </~~span~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4blm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blm OCA], [~~http~~://pdbe.org/4blm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4blm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4blm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4blm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4blm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blm OCA], [https://pdbe.org/4blm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4blm RCSB], [https://www.ebi.ac.uk/pdbsum/4blm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4blm ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/BLAC_BACLI BLAC_BACLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4blm ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4blm ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The crystallographic and molecular structure of the class A beta-lactamase (penicillinase) of Bacillus licheniformis 749/C has been refined with X-ray diffraction data to 2 A resolution. For the 27,330 data with F greater than or equal to 3 sigma(F), the R factor is 0.15; for all 30,090 data, R is 0.16. The estimated co-ordinate error is 0.15 A. In the final model, the deviation of covalent bonds and angles from ideality is 0.012 A and 2.2 degrees, respectively. The model includes two molecules of 29,500 daltons each in the asymmetric unit of space group P2(1), 484 water molecules and two tetrahedral buffer anions. Overlay of the two protein molecules results in a root-mean-square difference of 0.17 A and 0.41 A for alpha-carbon atoms and for all atoms, respectively. Twenty-six water molecules fall within 0.25 A of matching water molecules associated with the second protein molecule. The reactive Ser70 is on a turn of 3(10) helix at the N terminus of a longer alpha-helix (72-83). The penicillin-binding site near this helix contains at least seven water molecules. Upon penicillin entry, a water molecule in the oxyanion hole, hydrogen-bonded between the N terminus of helix (80-83) and beta-strand (230-238), would be displaced by the oxygen atom of the beta-lactam carbonyl group. An unexpelled molecule of water is proposed to be the catalytic water required for penicillin hydrolysis. The water is hydrogen-bonded to Glu166, a conserved residue in all beta-lactamases, and it lies 3 A from the alpha-face of a previously modeled penicillin. The position of the water-Glu166 pair is stabilized in the active site by a cis peptide bond at Pro167.

	~~Beta-lactamase of Bacillus licheniformis 749/C. Refinement at 2 A resolution and analysis of hydration.,Knox JR, Moews PC J Mol Biol. 1991 Jul 20;220(2):435-55. PMID:1856867<ref>PMID:1856867</ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4blm" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Beta-lactamase\|Beta-lactamase]]		*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Clostridium licheniforme weigmann 1898]]~~		[[Category: Bacillus licheniformis]]
	~~[[Category: Beta-lactamase~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Penicillin-binding Proteins]]		[[Category: Penicillin-binding Proteins]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Knox~~, J R~~]]		[[Category: Knox JR]]
	[[Category: Moews~~, P C~~]]		[[Category: Moews PC]]

OCA at 13:57, 13 March 2019

2019-03-13T13:57:03Z

← Older revision		Revision as of 13:57, 13 March 2019
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	==BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C. REFINEMENT AT 2 ANGSTROMS RESOLUTION AND ANALYSIS OF HYDRATION==		==BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C. REFINEMENT AT 2 ANGSTROMS RESOLUTION AND ANALYSIS OF HYDRATION==
	<StructureSection load='4blm' size='340' side='right' caption='[[4blm]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4blm' size='340' side='right'caption='[[4blm]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4blm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BLM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4blm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BLM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4blm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blm OCA], [http://pdbe.org/4blm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4blm RCSB], [http://www.ebi.ac.uk/pdbsum/4blm PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4blm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blm OCA], [http://pdbe.org/4blm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4blm RCSB], [http://www.ebi.ac.uk/pdbsum/4blm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4blm ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/4blm_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/4blm_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 36:
	[[Category: Clostridium licheniforme weigmann 1898]]		[[Category: Clostridium licheniforme weigmann 1898]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
			[[Category: Large Structures]]
	[[Category: Penicillin-binding Proteins]]		[[Category: Penicillin-binding Proteins]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Knox, J R]]		[[Category: Knox, J R]]
	[[Category: Moews, P C]]		[[Category: Moews, P C]]

OCA at 03:32, 9 February 2016

2016-02-09T03:32:42Z

← Older revision		Revision as of 03:32, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='4blm' size='340' side='right' caption='[[4blm]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4blm' size='340' side='right' caption='[[4blm]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4blm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_licheniformis Bacillus licheniformis~~]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BLM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4blm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BLM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4blm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4blm RCSB], [http://www.ebi.ac.uk/pdbsum/4blm PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4blm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blm OCA], [http://pdbe.org/4blm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4blm RCSB], [http://www.ebi.ac.uk/pdbsum/4blm PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4blm ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4blm" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus licheniformis~~]]		[[Category: Clostridium licheniforme weigmann 1898]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Penicillin-binding Proteins]]		[[Category: Penicillin-binding Proteins]]

OCA at 16:11, 9 December 2014

2014-12-09T16:11:22Z

← Older revision		Revision as of 16:11, 9 December 2014
Line 1:		Line 1:
	[[Image:~~4blm~~.~~png~~\|~~left~~\|~~200px~~]]		==BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C. REFINEMENT AT 2 ANGSTROMS RESOLUTION AND ANALYSIS OF HYDRATION==
			<StructureSection load='4blm' size='340' side='right' caption='[[4blm]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4blm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BLM FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4blm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4blm RCSB], [http://www.ebi.ac.uk/pdbsum/4blm PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/4blm_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The crystallographic and molecular structure of the class A beta-lactamase (penicillinase) of Bacillus licheniformis 749/C has been refined with X-ray diffraction data to 2 A resolution. For the 27,330 data with F greater than or equal to 3 sigma(F), the R factor is 0.15; for all 30,090 data, R is 0.16. The estimated co-ordinate error is 0.15 A. In the final model, the deviation of covalent bonds and angles from ideality is 0.012 A and 2.2 degrees, respectively. The model includes two molecules of 29,500 daltons each in the asymmetric unit of space group P2(1), 484 water molecules and two tetrahedral buffer anions. Overlay of the two protein molecules results in a root-mean-square difference of 0.17 A and 0.41 A for alpha-carbon atoms and for all atoms, respectively. Twenty-six water molecules fall within 0.25 A of matching water molecules associated with the second protein molecule. The reactive Ser70 is on a turn of 3(10) helix at the N terminus of a longer alpha-helix (72-83). The penicillin-binding site near this helix contains at least seven water molecules. Upon penicillin entry, a water molecule in the oxyanion hole, hydrogen-bonded between the N terminus of helix (80-83) and beta-strand (230-238), would be displaced by the oxygen atom of the beta-lactam carbonyl group. An unexpelled molecule of water is proposed to be the catalytic water required for penicillin hydrolysis. The water is hydrogen-bonded to Glu166, a conserved residue in all beta-lactamases, and it lies 3 A from the alpha-face of a previously modeled penicillin. The position of the water-Glu166 pair is stabilized in the active site by a cis peptide bond at Pro167.

	~~{{STRUCTURE_4blm\| PDB=4blm \| SCENE= }}~~		Beta-lactamase of Bacillus licheniformis 749/C. Refinement at 2 A resolution and analysis of hydration.,Knox JR, Moews PC J Mol Biol. 1991 Jul 20;220(2):435-55. PMID:1856867<ref>PMID:1856867</ref>

	~~===BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C. REFINEMENT AT 2 ANGSTROMS RESOLUTION AND ANALYSIS OF HYDRATION===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_1856867}}~~

	~~==About this Structure==~~
	~~[[4blm]] is~~ a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the ~~Month] feature on ''Penicillin-binding Proteins'' by David~~ S. ~~Goodsell is [http://dx~~.~~doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].~~

	==See Also==		==See Also==
	*[[Beta-lactamase\|Beta-lactamase]]		*[[Beta-lactamase\|Beta-lactamase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:001856867<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Bacillus licheniformis]]		[[Category: Bacillus licheniformis]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Penicillin-binding Proteins]]		[[Category: Penicillin-binding Proteins]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Knox, J R.]]		[[Category: Knox, J R]]
	[[Category: Moews, P C.]]		[[Category: Moews, P C]]

OCA at 12:29, 20 October 2012

2012-10-20T12:29:45Z

← Older revision		Revision as of 12:29, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[4blm]] is a 2 chain structure ~~of [[Beta-lactamase]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].		[[4blm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
	<ref group="xtra">PMID:001856867~~</ref><ref group="xtra">PMID:011170211</ref><ref group="xtra">PMID:012215419~~</ref><references group="xtra"/>		<ref group="xtra">PMID:001856867</ref><references group="xtra"/>
	[[Category: Bacillus licheniformis]]		[[Category: Bacillus licheniformis]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]

OCA: Protected "4blm" [edit=sysop:move=sysop]

2012-07-26T08:54:25Z

Protected "4blm" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:54, 26 July 2012
(No difference)

OCA at 08:54, 26 July 2012

2012-07-26T08:54:23Z

← Older revision		Revision as of 08:54, 26 July 2012
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:4blm.png\|left\|200px]]		[[Image:4blm.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_4blm", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_4blm\| PDB=4blm \| SCENE= }}		{{STRUCTURE_4blm\| PDB=4blm \| SCENE= }}

	===BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C. REFINEMENT AT 2 ANGSTROMS RESOLUTION AND ANALYSIS OF HYDRATION===		===BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C. REFINEMENT AT 2 ANGSTROMS RESOLUTION AND ANALYSIS OF HYDRATION===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_1856867}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 1856867 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_1856867}}		{{ABSTRACT_PUBMED_1856867}}

	==About this Structure==		==About this Structure==
	~~4BLM~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].		[[4blm]] is a 2 chain structure of [[Beta-lactamase]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].

			==See Also==
			*[[Beta-lactamase\|Beta-lactamase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~1856867~~</ref><references group="xtra"/>		<ref group="xtra">PMID:001856867</ref><ref group="xtra">PMID:011170211</ref><ref group="xtra">PMID:012215419</ref><references group="xtra"/>
	[[Category: Bacillus licheniformis]]		[[Category: Bacillus licheniformis]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
Line 30:		Line 21:
	[[Category: Knox, J R.]]		[[Category: Knox, J R.]]
	[[Category: Moews, P C.]]		[[Category: Moews, P C.]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 10:10:57 2010''~~

OCA at 08:10, 21 January 2010

2010-01-21T08:10:58Z

← Older revision		Revision as of 08:10, 21 January 2010
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	4BLM is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].		4BLM is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].

	==Reference==		==Reference==
Line 27:		Line 27:
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Penicillin-binding Proteins]]		[[Category: Penicillin-binding Proteins]]
			[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Knox, J R.]]		[[Category: Knox, J R.]]
	[[Category: Moews, P C.]]		[[Category: Moews, P C.]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 06~~:07:~~54 2009~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 10:10:57 2010''

OCA at 04:07, 17 February 2009

2009-02-17T04:07:57Z

← Older revision		Revision as of 04:07, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	4BLM is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. ~~Additional information on 4BLM is available in a page on~~ [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/~~pdb29_1~~.html Penicillin-binding Proteins~~] at the RCSB PDB~~ [http://~~pdb~~.~~rcsb~~.org/~~pdb~~/~~static~~.~~do?p=education_discussion~~/~~molecule_of_the_month~~/~~index.html Molecule of the Month~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].		4BLM is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. The May 2002 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Penicillin-binding Proteins'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2002_5 10.2210/rcsb_pdb/mom_2002_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].

	==Reference==		==Reference==
	~~Beta-lactamase of Bacillus licheniformis 749/C. Refinement at 2 A resolution and analysis of hydration., Knox JR, Moews PC, J Mol Biol. 1991 Jul 20;220(2):435-55.~~ PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~1856867 1856867]~~		<ref group="xtra">PMID:1856867</ref><references group="xtra"/>
	[[Category: Bacillus licheniformis]]		[[Category: Bacillus licheniformis]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Penicillin-binding Proteins]]		[[Category: Penicillin-binding Proteins]]
	~~[[Category: Single protein]]~~
	[[Category: Knox, J R.]]		[[Category: Knox, J R.]]
	[[Category: Moews, P C.]]		[[Category: Moews, P C.]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 23 11~~:11:~~39 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:07:54 2009''

OCA at 08:11, 23 July 2008

2008-07-23T08:11:39Z

← Older revision		Revision as of 08:11, 23 July 2008
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	4BLM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. ~~The following page contains interesting~~ information on ~~the relation of~~ 4BLM ~~with [~~[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb29_1.html Penicillin-binding Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].		4BLM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Additional information on 4BLM is available in a page on [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb29_1.html Penicillin-binding Proteins] at the RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLM OCA].

	==Reference==		==Reference==
Line 31:		Line 31:
	[[Category: Moews, P C.]]		[[Category: Moews, P C.]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Jul ~~3 13~~:30:06 2008''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 11:11:39 2008''