4bme - Revision history

OCA at 11:54, 20 December 2023

2023-12-20T11:54:30Z

← Older revision		Revision as of 11:54, 20 December 2023
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	<StructureSection load='4bme' size='340' side='right'caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4bme' size='340' side='right'caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bme]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BME FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bme]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BME FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLC:ALPHA-D~~-~~GLUCOSE'>GLC</scene>~~, ~~<scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height~~: ~~3em;~~'>~~[[4bmb\|4bmb]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900008:alpha-lactose'>PRD_900008</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bme OCA], [https://pdbe.org/4bme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bme RCSB], [https://www.ebi.ac.uk/pdbsum/4bme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bme ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bme OCA], [https://pdbe.org/4bme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bme RCSB], [https://www.ebi.ac.uk/pdbsum/4bme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bme ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN]] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>		[https://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Buzamet, E]]		[[Category: Buzamet E]]
	[[Category: Gabius~~, H J~~]]		[[Category: Gabius HJ]]
	[[Category: Menendez, M]]		[[Category: Menendez M]]
	[[Category: Romero, A]]		[[Category: Romero A]]
	[[Category: Ruiz~~, F M~~]]		[[Category: Ruiz FM]]
	[[Category: Solis, D]]		[[Category: Solis D]]
	~~[[Category: Carbohydrate recognition]]~~
	~~[[Category: Sugar binding protein~~]]

OCA at 07:18, 31 August 2022

2022-08-31T07:18:31Z

← Older revision		Revision as of 07:18, 31 August 2022
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	<StructureSection load='4bme' size='340' side='right'caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4bme' size='340' side='right'caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bme]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Human Human]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BME OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BME FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bme]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BME FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~LBT~~:ALPHA-~~LACTOSE~~'>~~LBT~~</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bmb\|4bmb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4bmb\|4bmb]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bme OCA], [~~http~~://pdbe.org/4bme PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bme RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bme PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bme ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bme OCA], [https://pdbe.org/4bme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bme RCSB], [https://www.ebi.ac.uk/pdbsum/4bme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bme ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN]] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>		[[https://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN]] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Galectin\|Galectin]]		*[[Galectin 3D structures\|Galectin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Human]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Buzamet, E]]		[[Category: Buzamet, E]]

OCA at 13:45, 13 March 2019

2019-03-13T13:45:17Z

← Older revision		Revision as of 13:45, 13 March 2019
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	==Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant==		==Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant==
	<StructureSection load='4bme' size='340' side='right' caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4bme' size='340' side='right'caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bme]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BME FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bme]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BME FirstGlance]. <br>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Buzamet, E]]		[[Category: Buzamet, E]]
	[[Category: Gabius, H J]]		[[Category: Gabius, H J]]

OCA at 08:54, 5 August 2016

2016-08-05T08:54:22Z

← Older revision		Revision as of 08:54, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant==		==Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant==
	<StructureSection load='4bme' size='340' side='right' caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4bme' size='340' side='right' caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LBT:ALPHA-LACTOSE'>LBT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LBT:ALPHA-LACTOSE'>LBT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bmb\|4bmb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bmb\|4bmb]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bme OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bme RCSB], [http://www.ebi.ac.uk/pdbsum/4bme PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bme OCA], [http://pdbe.org/4bme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bme RCSB], [http://www.ebi.ac.uk/pdbsum/4bme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bme ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4bme" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Galectin\|Galectin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:26, 25 December 2014

2014-12-25T17:26:51Z

← Older revision		Revision as of 17:26, 25 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4bme\| PDB=4bme \| SCENE= }}~~		==Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant==
	===Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant===		<StructureSection load='4bme' size='340' side='right' caption='[[4bme]], [[Resolution\|resolution]] 2.00Å' scene=''>
	~~{{ABSTRACT_PUBMED_24418318}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4bme]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BME FirstGlance]. <br>
	==Function==		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LBT:ALPHA-LACTOSE'>LBT</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bmb\|4bmb]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bme OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bme RCSB], [http://www.ebi.ac.uk/pdbsum/4bme PDBsum]</span></td></tr>
			</table>
			== Function ==
	[[http://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN]] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>		[[http://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN]] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Natural amino acid substitution by single-site nucleotide polymorphism can become a valuable tool for structure-activity correlations, especially if evidence for association to disease parameters exists. Focusing on the F19Y change in human galectin-8, connected clinically to rheumatoid arthritis, we here initiate the study of consequences of a single-site substitution in the carbohydrate recognition domain of this family of cellular effectors. We apply a strategically combined set of structural and cell biological techniques for comparing properties of the wild-type and variant proteins. The overall hydrodynamic behavior of the full-length protein and of the separate N-domain is not noticeably altered, but displacements in the F0 beta-strand of the beta-sandwich fold in the N-domain are induced, as evidenced by protein crystallography. Analysis of thermal stability by circular dichroism spectroscopy revealed perceptible differences for the full-length proteins, pointing to an impact of the substitution beyond the N-domain. In addition, small differences in thermodynamic parameters of carbohydrate binding are detected. On the level of two types of tumor cells, characteristics of binding appeared rather similar. In further comparison of the influence on proliferation, the variant proved to be more active as growth regulator in the six tested lines of neuroblastoma, erythroleukemia and colon adenocarcinoma. The seemingly subtle structural change identified here thus has functional implications in vitro, encouraging further analysis in autoimmune regulation and, in a broad context, in work with other natural single-site variants, using the documented combined strategy. DATABASE: The atomic coordinates and structure factors (codes 4BMB, 4BME) have been deposited in the Protein Data Bank.

	~~==About this Structure==~~		Natural single amino acid polymorphism (F19Y) in human galectin-8: detection of structural alterations and increased growth-regulatory activity on tumor cells.,Ruiz FM, Scholz BA, Buzamet E, Kopitz J, Andre S, Menendez M, Romero A, Solis D, Gabius HJ FEBS J. 2014 Mar;281(5):1446-64. doi: 10.1111/febs.12716. Epub 2014 Feb 3. PMID:24418318<ref>PMID:24418318</ref>
	~~[[4bme]] is a 2 chain structure~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4BME OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:024418318</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
	[[Category: Buzamet, E.]]		== References ==
	[[Category: Gabius, H J.]]		<references/>
	[[Category: Menendez, M.]]		__TOC__
	[[Category: Romero, A.]]		</StructureSection>
	[[Category: Ruiz, F M.]]		[[Category: Human]]
	[[Category: Solis, D.]]		[[Category: Buzamet, E]]
			[[Category: Gabius, H J]]
			[[Category: Menendez, M]]
			[[Category: Romero, A]]
			[[Category: Ruiz, F M]]
			[[Category: Solis, D]]
	[[Category: Carbohydrate recognition]]		[[Category: Carbohydrate recognition]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

OCA at 08:42, 19 March 2014

2014-03-19T08:42:15Z

← Older revision		Revision as of 08:42, 19 March 2014
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	~~'''Unreleased~~ structure~~'''~~		{{STRUCTURE_4bme\| PDB=4bme \| SCENE= }}
			===Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant===
			{{ABSTRACT_PUBMED_24418318}}

	~~The entry 4bme is ON HOLD~~ ~~until Paper Publication~~		==Function==
			[[http://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN]] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>

	~~Authors~~: ~~Buzamet, E., Ruiz, F.M., Menendez, M., Romero, A~~.~~, Gabius, H~~.J.~~, Solis, D~~.		==About this Structure==
			[[4bme]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BME OCA].

	~~Description~~: ~~Crystal structure of the N terminal domain of human Galectin 8~~, ~~F19Y mutant~~		==Reference==
			<ref group="xtra">PMID:024418318</ref><references group="xtra"/><references/>
			[[Category: Buzamet, E.]]
			[[Category: Gabius, H J.]]
			[[Category: Menendez, M.]]
			[[Category: Romero, A.]]
			[[Category: Ruiz, F M.]]
			[[Category: Solis, D.]]
			[[Category: Carbohydrate recognition]]
			[[Category: Sugar binding protein]]

OCA at 10:47, 19 June 2013

2013-06-19T10:47:09Z

← Older revision		Revision as of 10:47, 19 June 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4bme is ON HOLD		The entry 4bme is ON HOLD until Paper Publication

	Authors: Buzamet, E., Ruiz, F.M., Menendez, M., Romero, A., Gabius, H.J., Solis, D.		Authors: Buzamet, E., Ruiz, F.M., Menendez, M., Romero, A., Gabius, H.J., Solis, D.

	Description: Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant		Description: Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant

OCA: Protected "4bme" [edit=sysop:move=sysop]

2013-05-16T06:42:33Z

Protected "4bme" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:42, 16 May 2013
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4bme is ON HOLD Authors: Buzamet, E., Ruiz, F.M., Menendez, M., Romero, A., Gabius, H.J., Solis, D. Description: Crystal structure of the N termina...

2013-05-16T06:42:32Z

New page: '''Unreleased structure''' The entry 4bme is ON HOLD Authors: Buzamet, E., Ruiz, F.M., Menendez, M., Romero, A., Gabius, H.J., Solis, D. Description: Crystal structure of the N termina...

New page

'''Unreleased structure'''

The entry 4bme is ON HOLD

Authors: Buzamet, E., Ruiz, F.M., Menendez, M., Romero, A., Gabius, H.J., Solis, D.

Description: Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant