4bs0 - Revision history

OCA at 11:57, 20 December 2023

2023-12-20T11:57:16Z

← Older revision		Revision as of 11:57, 20 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6NT:6-NITROBENZOTRIAZOLE'>6NT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.09Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6NT:6-NITROBENZOTRIAZOLE'>6NT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bs0 OCA], [https://pdbe.org/4bs0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bs0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bs0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bs0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bs0 OCA], [https://pdbe.org/4bs0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bs0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bs0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bs0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/XYNA_THEAU XYNA_THEAU]]		[https://www.uniprot.org/uniprot/XYNA_THEAU XYNA_THEAU]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of design to one of molecular recognition. Nevertheless, past attempts to capitalize on this idea, for example by using transition state analogues to elicit antibodies with catalytic activities, have generally failed to deliver true enzymatic rates. The advent of computational design approaches, combined with directed evolution, has provided an opportunity to revisit this problem. Starting from a computationally designed catalyst for the Kemp elimination-a well-studied model system for proton transfer from carbon-we show that an artificial enzyme can be evolved that accelerates an elementary chemical reaction 6 x 108-fold, approaching the exceptional efficiency of highly optimized natural enzymes such as triosephosphate isomerase. A 1.09 A resolution crystal structure of the evolved enzyme indicates that familiar catalytic strategies such as shape complementarity and precisely placed catalytic groups can be successfully harnessed to afford such high rate accelerations, making us optimistic about the prospects of designing more sophisticated catalysts.

			Precision is essential for efficient catalysis in an evolved Kemp eliminase.,Blomberg R, Kries H, Pinkas DM, Mittl PR, Grutter MG, Privett HK, Mayo SL, Hilvert D Nature. 2013 Oct 16. doi: 10.1038/nature12623. PMID:24132235<ref>PMID:24132235</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4bs0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Kemp eliminase\|Kemp eliminase]]		*[[Kemp eliminase\|Kemp eliminase]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:03, 7 September 2022

2022-09-07T17:03:27Z

← Older revision		Revision as of 17:03, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4bs0' size='340' side='right'caption='[[4bs0]], [[Resolution\|resolution]] 1.09Å' scene=''>		<StructureSection load='4bs0' size='340' side='right'caption='[[4bs0]], [[Resolution\|resolution]] 1.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Theau Theau~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6NT:6-NITROBENZOTRIAZOLE'>6NT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6NT:6-NITROBENZOTRIAZOLE'>6NT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bs0 OCA], [~~http~~://pdbe.org/4bs0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bs0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bs0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bs0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bs0 OCA], [https://pdbe.org/4bs0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bs0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bs0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bs0 ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[[https://www.uniprot.org/uniprot/XYNA_THEAU XYNA_THEAU]]
	Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of design to one of molecular recognition. Nevertheless, past attempts to capitalize on this idea, for example by using transition state analogues to elicit antibodies with catalytic activities, have generally failed to deliver true enzymatic rates. The advent of computational design approaches, combined with directed evolution, has provided an opportunity to revisit this problem. Starting from a computationally designed catalyst for the Kemp elimination-a well-studied model system for proton transfer from carbon-we show that an artificial enzyme can be evolved that accelerates an elementary chemical reaction 6 x 108-fold, approaching the exceptional efficiency of highly optimized natural enzymes such as triosephosphate isomerase. A 1.09 A resolution crystal structure of the evolved enzyme indicates that familiar catalytic strategies such as shape complementarity and precisely placed catalytic groups can be successfully harnessed to afford such high rate accelerations, making us optimistic about the prospects of designing more sophisticated catalysts.

	~~Precision is essential for efficient catalysis in an evolved Kemp eliminase.,Blomberg R, Kries H, Pinkas DM, Mittl PR, Grutter MG, Privett HK, Mayo SL, Hilvert D Nature. 2013 Oct 16. doi~~: ~~10.1038~~/~~nature12623. PMID:24132235<ref>PMID:24132235</ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 4bs0" style="background-color:#fffaf0;"><~~/~~div>~~

	==See Also==		==See Also==
	*[[Kemp eliminase\|Kemp eliminase]]		*[[Kemp eliminase\|Kemp eliminase]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Theau~~]]		[[Category: Thermoascus aurantiacus]]
	[[Category: Blomberg, R]]		[[Category: Blomberg R]]
	[[Category: Gruetter~~, M G~~]]		[[Category: Gruetter MG]]
	[[Category: Hilvert, D]]		[[Category: Hilvert D]]
	[[Category: Kries, H]]		[[Category: Kries H]]
	[[Category: Mayo, S]]		[[Category: Mayo S]]
	[[Category: Mittl~~, P R.E~~]]		[[Category: Mittl PRE]]
	[[Category: Pinkas~~, D M~~]]		[[Category: Pinkas DM]]
	[[Category: Privett~~, H K]]~~		[[Category: Privett HK]]
	~~[[Category: Bottom-up enzyme construction]]~~
	~~[[Category: Computational protein design]]~~
	~~[[Category: Directed evolution]]~~
	~~[[Category: Elementary chemical step catalysis]]~~
	~~[[Category: Kemp elimination]]~~
	~~[[Category: Lyase-lyase inhibitor complex]]~~
	~~[[Category: Proton transfer]]~~
	~~[[Category: Transition state tuning~~]]

OCA at 13:08, 13 March 2019

2019-03-13T13:08:17Z

← Older revision		Revision as of 13:08, 13 March 2019
Line 1:		Line 1:

	==Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole==		==Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole==
	<StructureSection load='4bs0' size='340' side='right' caption='[[4bs0]], [[Resolution\|resolution]] 1.09Å' scene=''>		<StructureSection load='4bs0' size='340' side='right'caption='[[4bs0]], [[Resolution\|resolution]] 1.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theau Theau]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theau Theau]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Theau]]		[[Category: Theau]]
	[[Category: Blomberg, R]]		[[Category: Blomberg, R]]

OCA at 11:07, 4 August 2016

2016-08-04T11:07:17Z

← Older revision		Revision as of 11:07, 4 August 2016
Line 1:		Line 1:

	==Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole==		==Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole==
	<StructureSection load='4bs0' size='340' side='right' caption='[[4bs0]], [[Resolution\|resolution]] 1.09Å' scene=''>		<StructureSection load='4bs0' size='340' side='right' caption='[[4bs0]], [[Resolution\|resolution]] 1.09Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theau Theau]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theau Theau]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6NT:6-NITROBENZOTRIAZOLE'>6NT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6NT:6-NITROBENZOTRIAZOLE'>6NT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bs0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bs0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bs0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bs0 OCA], [http://pdbe.org/4bs0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bs0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bs0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bs0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4bs0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 17:58, 21 December 2014

2014-12-21T17:58:23Z

← Older revision		Revision as of 17:58, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4bs0\| PDB=4bs0 \| SCENE= }}~~		==Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole==
	===Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole===		<StructureSection load='4bs0' size='340' side='right' caption='[[4bs0]], [[Resolution\|resolution]] 1.09Å' scene=''>
	~~{{ABSTRACT_PUBMED_24132235}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theau Theau]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BS0 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6NT:6-NITROBENZOTRIAZOLE'>6NT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bs0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bs0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bs0 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of design to one of molecular recognition. Nevertheless, past attempts to capitalize on this idea, for example by using transition state analogues to elicit antibodies with catalytic activities, have generally failed to deliver true enzymatic rates. The advent of computational design approaches, combined with directed evolution, has provided an opportunity to revisit this problem. Starting from a computationally designed catalyst for the Kemp elimination-a well-studied model system for proton transfer from carbon-we show that an artificial enzyme can be evolved that accelerates an elementary chemical reaction 6 x 108-fold, approaching the exceptional efficiency of highly optimized natural enzymes such as triosephosphate isomerase. A 1.09 A resolution crystal structure of the evolved enzyme indicates that familiar catalytic strategies such as shape complementarity and precisely placed catalytic groups can be successfully harnessed to afford such high rate accelerations, making us optimistic about the prospects of designing more sophisticated catalysts.

	~~==About this Structure==~~		Precision is essential for efficient catalysis in an evolved Kemp eliminase.,Blomberg R, Kries H, Pinkas DM, Mittl PR, Grutter MG, Privett HK, Mayo SL, Hilvert D Nature. 2013 Oct 16. doi: 10.1038/nature12623. PMID:24132235<ref>PMID:24132235</ref>
	~~[[4bs0]]~~ is ~~a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Theau Theau]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann.ac.il~~/~~oca-bin/ocashort?id=4BS0 OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group~~=~~"xtra">PMID:024132235</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>

			==See Also==
			*[[Kemp eliminase\|Kemp eliminase]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Theau]]		[[Category: Theau]]
	[[Category: Blomberg, R.]]		[[Category: Blomberg, R]]
	[[Category: Gruetter, M G.]]		[[Category: Gruetter, M G]]
	[[Category: Hilvert, D.]]		[[Category: Hilvert, D]]
	[[Category: Kries, H.]]		[[Category: Kries, H]]
	[[Category: Mayo, S.]]		[[Category: Mayo, S]]
	[[Category: Mittl, P R.E.]]		[[Category: Mittl, P R.E]]
	[[Category: Pinkas, D M.]]		[[Category: Pinkas, D M]]
	[[Category: Privett, H K.]]		[[Category: Privett, H K]]
	[[Category: Bottom-up enzyme construction]]		[[Category: Bottom-up enzyme construction]]
	[[Category: Computational protein design]]		[[Category: Computational protein design]]

OCA at 08:59, 27 November 2013

2013-11-27T08:59:55Z

← Older revision		Revision as of 08:59, 27 November 2013
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
	[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermoascus_aurantiacus Thermoascus aurantiacus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA].		[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theau Theau]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024132235</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024132235</ref><references group="xtra"/><references/>
	[[Category: ~~Thermoascus aurantiacus~~]]		[[Category: Theau]]
	[[Category: Blomberg, R.]]		[[Category: Blomberg, R.]]
	[[Category: Gruetter, M G.]]		[[Category: Gruetter, M G.]]

OCA at 08:23, 30 October 2013

2013-10-30T08:23:46Z

← Older revision		Revision as of 08:23, 30 October 2013
Line 1:		Line 1:
	{{STRUCTURE_4bs0\| PDB=4bs0 \| SCENE= }}		{{STRUCTURE_4bs0\| PDB=4bs0 \| SCENE= }}
	===Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole===		===Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole===
			{{ABSTRACT_PUBMED_24132235}}

	==About this Structure==		==About this Structure==
	[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA].		[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA].

			==Reference==
			<ref group="xtra">PMID:024132235</ref><references group="xtra"/><references/>
	[[Category: Thermoascus aurantiacus]]		[[Category: Thermoascus aurantiacus]]
	[[Category: Blomberg, R.]]		[[Category: Blomberg, R.]]

OCA at 08:05, 16 October 2013

2013-10-16T08:05:47Z

← Older revision		Revision as of 08:05, 16 October 2013
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		{{STRUCTURE_4bs0\| PDB=4bs0 \| SCENE= }}
			===Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole===

	~~The entry~~ 4bs0 is ~~ON HOLD until Paper Publication~~		==About this Structure==
			[[4bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BS0 OCA].
	~~Authors~~: Blomberg, R., Kries, H., ~~Pinkas, D~~.~~M.,~~ Mittl, P.R.E., ~~Gruetter,~~ M.~~G.,~~ Privett, H.K.~~, Mayo, S., Hilvert, D.~~		[[Category: Thermoascus aurantiacus]]
			[[Category: Blomberg, R.]]
	~~Description~~: ~~Crystal Structure of~~ Kemp ~~Eliminase HG3.17 Complexed with~~ Transition ~~State Analog 6-Nitrobenzotriazole~~		[[Category: Gruetter, M G.]]
			[[Category: Hilvert, D.]]
			[[Category: Kries, H.]]
			[[Category: Mayo, S.]]
			[[Category: Mittl, P R.E.]]
			[[Category: Pinkas, D M.]]
			[[Category: Privett, H K.]]
			[[Category: Bottom-up enzyme construction]]
			[[Category: Computational protein design]]
			[[Category: Directed evolution]]
			[[Category: Elementary chemical step catalysis]]
			[[Category: Kemp elimination]]
			[[Category: Lyase-lyase inhibitor complex]]
			[[Category: Proton transfer]]
			[[Category: Transition state tuning]]

OCA at 09:28, 4 September 2013

2013-09-04T09:28:38Z

← Older revision		Revision as of 09:28, 4 September 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4bs0 is ON HOLD		The entry 4bs0 is ON HOLD until Paper Publication

	Authors: Blomberg, R., Kries, H., Pinkas, D.M., Mittl, P.R.E., Gruetter, M.G., Privett, H.K., Mayo, S., Hilvert, D.		Authors: Blomberg, R., Kries, H., Pinkas, D.M., Mittl, P.R.E., Gruetter, M.G., Privett, H.K., Mayo, S., Hilvert, D.

	Description: Crystal Structure of Kemp Eliminase HG3.17 Complexed with Transition State Analog 6-Nitrobenzotriazole		Description: Crystal Structure of Kemp Eliminase HG3.17 Complexed with Transition State Analog 6-Nitrobenzotriazole

OCA: Protected "4bs0" [edit=sysop:move=sysop]

2013-06-19T10:56:29Z

Protected "4bs0" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:56, 19 June 2013
(No difference)