4bvs - Revision history

OCA at 11:58, 20 December 2023

2023-12-20T11:58:59Z

← Older revision		Revision as of 11:58, 20 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BVS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BVS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AX2:1,3,5-TRIAZINE-2,4,6-TRIAMINE'>AX2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AX2:1,3,5-TRIAZINE-2,4,6-TRIAMINE'>AX2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bvs OCA], [https://pdbe.org/4bvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bvs RCSB], [https://www.ebi.ac.uk/pdbsum/4bvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bvs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bvs OCA], [https://pdbe.org/4bvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bvs RCSB], [https://www.ebi.ac.uk/pdbsum/4bvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bvs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/CAH_PSESD CAH_PSESD]] Responsible for the hydrolysis of cyanuric acid, an intermediate formed during catabolism of s-triazine based compounds in herbicides such as atrazine and polymers such as melamine. Catalyzes the hydrolytic opening of the s-triazine ring of cyanuric acid (2,4,6-trihydroxy-s-triazine) to yield carbon dioxide and carboxybiuret, which spontaneously decarboxylates to biuret.[HAMAP-Rule:MF_01989]<ref>PMID:11544232</ref>		[https://www.uniprot.org/uniprot/CAH_PSESD CAH_PSESD] Responsible for the hydrolysis of cyanuric acid, an intermediate formed during catabolism of s-triazine based compounds in herbicides such as atrazine and polymers such as melamine. Catalyzes the hydrolytic opening of the s-triazine ring of cyanuric acid (2,4,6-trihydroxy-s-triazine) to yield carbon dioxide and carboxybiuret, which spontaneously decarboxylates to biuret.[HAMAP-Rule:MF_01989]<ref>PMID:11544232</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The cyanuric acid hydrolase, AtzD, is the founding member of a newly identified family of ring-opening amidases. We report the first X-ray structure for this family, which is a novel fold (termed the 'Toblerone' fold) that likely evolved via the concatenation of monomers of the trimeric YjgF superfamily and the acquisition of a metal binding site. Structures of AtzD with bound substrate (cyanuric acid) and inhibitors (phosphate, barbituric acid and melamine), along with mutagenesis studies, allowed the identification of the active site. The AtzD monomer, active site and substrate all possess threefold rotational symmetry, to the extent that the active site possesses three potential Ser-Lys catalytic dyads. A single catalytic dyad (Ser85-Lys42) is hypothesized, based on biochemical evidence and crystallographic data. A plausible catalytic mechanism based on these observations is also presented. A comparison with a homology model of the related barbiturase, Bar, was used to infer the active-site residues responsible for substrate specificity, and the phylogeny of the 68 AtzD-like enzymes in the database were analysed in light of this structure-function relationship.

			Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.,Peat TS, Balotra S, Wilding M, French NG, Briggs LJ, Panjikar S, Cowieson N, Newman J, Scott C Mol Microbiol. 2013 Jun;88(6):1149-63. doi: 10.1111/mmi.12249. Epub 2013 May 20. PMID:23651355<ref>PMID:23651355</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4bvs" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:11, 7 September 2022

2022-09-07T17:11:31Z

← Older revision		Revision as of 17:11, 7 September 2022
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	<StructureSection load='4bvs' size='340' side='right'caption='[[4bvs]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4bvs' size='340' side='right'caption='[[4bvs]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Psesd Psesd~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BVS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BVS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AX2:1,3,5-TRIAZINE-2,4,6-TRIAMINE'>AX2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AX2:1,3,5-TRIAZINE-2,4,6-TRIAMINE'>AX2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bvq\|4bvq]], [[4bvr\|4bvr]], [[4bvt\|4bvt]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bvs OCA], [https://pdbe.org/4bvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bvs RCSB], [https://www.ebi.ac.uk/pdbsum/4bvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bvs ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyanuric_acid_amidohydrolase Cyanuric acid amidohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.15 3.5.2.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bvs OCA], [~~http~~://pdbe.org/4bvs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bvs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bvs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bvs ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	=~~= Publication Abstract from PubMed~~ ==		[[https://www.uniprot.org/uniprot/CAH_PSESD CAH_PSESD]] Responsible for the hydrolysis of cyanuric acid, an intermediate formed during catabolism of s-triazine based compounds in herbicides such as atrazine and polymers such as melamine. Catalyzes the hydrolytic opening of the s-triazine ring of cyanuric acid (2,4,6-trihydroxy-s-triazine) to yield carbon dioxide and carboxybiuret, which spontaneously decarboxylates to biuret.[HAMAP-Rule:MF_01989]<ref>PMID:11544232</ref>
	~~The~~ cyanuric acid ~~hydrolase, AtzD~~, ~~is the founding member of a newly identified family~~ of ~~ring~~-~~opening amidases~~. ~~We report~~ the ~~first X-ray structure for this family, which is a novel fold (termed the 'Toblerone' fold) that likely evolved via the concatenation of monomers~~ of the ~~trimeric YjgF superfamily and the acquisition~~ of ~~a metal binding site. Structures of AtzD with bound substrate (~~cyanuric acid~~) and inhibitors~~ (~~phosphate, barbituric acid and melamine)~~, ~~along with mutagenesis studies~~, ~~allowed the identification of the active site. The AtzD monomer, active site and substrate all possess threefold rotational symmetry, to the extent that the active site possesses three potential Ser~~-~~Lys catalytic dyads. A single catalytic dyad (Ser85~~-~~Lys42~~) ~~is hypothesized, based on biochemical evidence~~ and ~~crystallographic data. A plausible catalytic mechanism based on these observations is also presented. A comparison with a homology model of the related barbiturase~~, ~~Bar, was used~~ to ~~infer the active-site residues responsible for substrate specificity, and the phylogeny of the 68 AtzD-like enzymes in the database were analysed in light of this structure-function relationship~~.

	Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.,Peat TS, Balotra S, Wilding M, French NG, Briggs LJ, Panjikar S, Cowieson N, Newman J, Scott C Mol Microbiol. 2013 Jun;88(6):1149-~~63. doi~~: ~~10.1111/mmi.12249. Epub 2013 May 20. PMID:23651355~~<ref>PMID:~~23651355~~</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4bvs" style="background-color:#fffaf0;"></div~~>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Cyanuric acid amidohydrolase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Psesd~~]]		[[Category: Pseudomonas sp. ADP]]
	[[Category: Balotra, S]]		[[Category: Balotra S]]
	[[Category: Briggs~~, L J~~]]		[[Category: Briggs LJ]]
	[[Category: Cowieson, N]]		[[Category: Cowieson N]]
	[[Category: French~~, N G~~]]		[[Category: French NG]]
	[[Category: Newman, J]]		[[Category: Newman J]]
	[[Category: Panjikar, S]]		[[Category: Panjikar S]]
	[[Category: Peat~~, T S~~]]		[[Category: Peat TS]]
	[[Category: Scott, C]]		[[Category: Scott C]]
	[[Category: Wilding, M]]		[[Category: Wilding M]]
	~~[[Category: Amidase]]~~
	~~[[Category: Hydrolase~~]]

OCA at 13:02, 13 March 2019

2019-03-13T13:02:19Z

← Older revision		Revision as of 13:02, 13 March 2019
Line 1:		Line 1:

	==Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.==		==Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.==
	<StructureSection load='4bvs' size='340' side='right' caption='[[4bvs]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4bvs' size='340' side='right'caption='[[4bvs]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psesd Psesd]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BVS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psesd Psesd]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BVS FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Cyanuric acid amidohydrolase]]		[[Category: Cyanuric acid amidohydrolase]]
			[[Category: Large Structures]]
	[[Category: Psesd]]		[[Category: Psesd]]
	[[Category: Balotra, S]]		[[Category: Balotra, S]]

OCA at 13:21, 11 August 2016

2016-08-11T13:21:08Z

← Older revision		Revision as of 13:21, 11 August 2016
Line 1:		Line 1:

	==Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.==		==Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.==
	<StructureSection load='4bvs' size='340' side='right' caption='[[4bvs]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4bvs' size='340' side='right' caption='[[4bvs]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_sp._adp Pseudomonas sp. adp~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BVS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psesd Psesd]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BVS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AX2:1,3,5-TRIAZINE-2,4,6-TRIAMINE'>AX2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AX2:1,3,5-TRIAZINE-2,4,6-TRIAMINE'>AX2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bvq\|4bvq]], [[4bvr\|4bvr]], [[4bvt\|4bvt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bvq\|4bvq]], [[4bvr\|4bvr]], [[4bvt\|4bvt]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyanuric_acid_amidohydrolase Cyanuric acid amidohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.15 3.5.2.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyanuric_acid_amidohydrolase Cyanuric acid amidohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.15 3.5.2.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bvs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bvs RCSB], [http://www.ebi.ac.uk/pdbsum/4bvs PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bvs OCA], [http://pdbe.org/4bvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bvs RCSB], [http://www.ebi.ac.uk/pdbsum/4bvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bvs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4bvs" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Cyanuric acid amidohydrolase]]		[[Category: Cyanuric acid amidohydrolase]]
	[[Category: ~~Pseudomonas sp. adp~~]]		[[Category: Psesd]]
	[[Category: Balotra, S]]		[[Category: Balotra, S]]
	[[Category: Briggs, L J]]		[[Category: Briggs, L J]]

OCA at 13:38, 4 January 2015

2015-01-04T13:38:34Z

← Older revision		Revision as of 13:38, 4 January 2015
Line 1:		Line 1:
	~~{{STRUCTURE_4bvs~~\| PDB=~~4bvs~~ \| ~~SCENE~~= }}		==Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.==
	===Cyanuric acid ~~hydrolase~~: ~~evolutionary innovation by structural concatenation~~.===		<StructureSection load='4bvs' size='340' side='right' caption='[[4bvs]], [[Resolution\|resolution]] 2.60Å' scene=''>
	~~{{ABSTRACT_PUBMED_23651355}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4bvs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._adp Pseudomonas sp. adp]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BVS FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AX2:1,3,5-TRIAZINE-2,4,6-TRIAMINE'>AX2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bvq\|4bvq]], [[4bvr\|4bvr]], [[4bvt\|4bvt]]</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyanuric_acid_amidohydrolase Cyanuric acid amidohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.15 3.5.2.15] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bvs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bvs RCSB], [http://www.ebi.ac.uk/pdbsum/4bvs PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The cyanuric acid hydrolase, AtzD, is the founding member of a newly identified family of ring-opening amidases. We report the first X-ray structure for this family, which is a novel fold (termed the 'Toblerone' fold) that likely evolved via the concatenation of monomers of the trimeric YjgF superfamily and the acquisition of a metal binding site. Structures of AtzD with bound substrate (cyanuric acid) and inhibitors (phosphate, barbituric acid and melamine), along with mutagenesis studies, allowed the identification of the active site. The AtzD monomer, active site and substrate all possess threefold rotational symmetry, to the extent that the active site possesses three potential Ser-Lys catalytic dyads. A single catalytic dyad (Ser85-Lys42) is hypothesized, based on biochemical evidence and crystallographic data. A plausible catalytic mechanism based on these observations is also presented. A comparison with a homology model of the related barbiturase, Bar, was used to infer the active-site residues responsible for substrate specificity, and the phylogeny of the 68 AtzD-like enzymes in the database were analysed in light of this structure-function relationship.

	~~==About this Structure==~~		Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.,Peat TS, Balotra S, Wilding M, French NG, Briggs LJ, Panjikar S, Cowieson N, Newman J, Scott C Mol Microbiol. 2013 Jun;88(6):1149-63. doi: 10.1111/mmi.12249. Epub 2013 May 20. PMID:23651355<ref>PMID:23651355</ref>
	~~[[4bvs]] is a 2 chain structure with sequence from [http~~:~~//en.wikipedia~~.~~org/wiki/Pseudomonas_sp~~.~~_adp Pseudomonas sp. adp]. This structure supersedes the now removed PDB entry [http~~:~~//oca.weizmann.ac.il/oca-bin/send~~-~~pdb?obs=1&id=3zgt 3zgt]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4BVS OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:023651355</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Cyanuric acid amidohydrolase]]		[[Category: Cyanuric acid amidohydrolase]]
	[[Category: Pseudomonas sp. adp]]		[[Category: Pseudomonas sp. adp]]
	[[Category: Balotra, S.]]		[[Category: Balotra, S]]
	[[Category: Briggs, L J.]]		[[Category: Briggs, L J]]
	[[Category: Cowieson, N.]]		[[Category: Cowieson, N]]
	[[Category: French, N G.]]		[[Category: French, N G]]
	[[Category: Newman, J.]]		[[Category: Newman, J]]
	[[Category: Panjikar, S.]]		[[Category: Panjikar, S]]
	[[Category: Peat, T S.]]		[[Category: Peat, T S]]
	[[Category: Scott, C.]]		[[Category: Scott, C]]
	[[Category: Wilding, M.]]		[[Category: Wilding, M]]
	[[Category: Amidase]]		[[Category: Amidase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 04:53, 18 July 2013

2013-07-18T04:53:29Z

← Older revision		Revision as of 04:53, 18 July 2013
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		{{STRUCTURE_4bvs\| PDB=4bvs \| SCENE= }}
			===Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.===
			{{ABSTRACT_PUBMED_23651355}}

	~~The~~ entry ~~4bvs~~ is ~~ON HOLD~~		==About this Structure==
			[[4bvs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._adp Pseudomonas sp. adp]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3zgt 3zgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BVS OCA].

	~~Authors~~: ~~Peat, T~~.~~S.,~~ Balotra, S., ~~Wilding~~, M., French, N.G., ~~Briggs, L.~~J., Panjikar, S., ~~Cowieson, N~~.~~, Newman, J.,~~ Scott, C.		==Reference==
			<ref group="xtra">PMID:023651355</ref><references group="xtra"/><references/>
	~~Description~~: ~~Cyanuric acid hydrolase~~: ~~evolutionary innovation by structural concatenation.~~		[[Category: Cyanuric acid amidohydrolase]]
			[[Category: Pseudomonas sp. adp]]
			[[Category: Balotra, S.]]
			[[Category: Briggs, L J.]]
			[[Category: Cowieson, N.]]
			[[Category: French, N G.]]
			[[Category: Newman, J.]]
			[[Category: Panjikar, S.]]
			[[Category: Peat, T S.]]
			[[Category: Scott, C.]]
			[[Category: Wilding, M.]]
			[[Category: Amidase]]
			[[Category: Hydrolase]]

OCA: Protected "4bvs" [edit=sysop:move=sysop]

2013-07-03T12:54:18Z

Protected "4bvs" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:54, 3 July 2013
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4bvs is ON HOLD Authors: Peat, T.S., Balotra, S., Wilding, M., French, N.G., Briggs, L.J., Panjikar, S., Cowieson, N., Newman, J., Scott, C. Descri...

2013-07-03T12:54:16Z

New page: '''Unreleased structure''' The entry 4bvs is ON HOLD Authors: Peat, T.S., Balotra, S., Wilding, M., French, N.G., Briggs, L.J., Panjikar, S., Cowieson, N., Newman, J., Scott, C. Descri...

New page

'''Unreleased structure'''

The entry 4bvs is ON HOLD

Authors: Peat, T.S., Balotra, S., Wilding, M., French, N.G., Briggs, L.J., Panjikar, S., Cowieson, N., Newman, J., Scott, C.

Description: Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.