4cbf - Revision history

OCA at 11:10, 9 May 2024

2024-05-09T11:10:53Z

← Older revision		Revision as of 11:10, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [https://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [https://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [https://pdbe.org/4cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [https://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.1Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [https://pdbe.org/4cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [https://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/E0WXI2_9FLAV E0WXI2_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS011998_004_099774]		[https://www.uniprot.org/uniprot/E0WXI2_9FLAV E0WXI2_9FLAV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS011998_004_099774]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Dengue virus (DENV), a mosquito-borne virus, is responsible for millions cases of infections worldwide. There are four DENV serotypes (DENV1 - 4). After a primary DENV infection, the antibodies elicited confer lifetime protection against that DENV serotype. However, in a secondary infection with another serotype, the pre-existing antibodies may cause antibody-dependent enhancement (ADE) of infection of macrophage cells leading to the development of the more severe form of disease, dengue hemorrhagic fever. Thus a safe vaccine should stimulate protection against all dengue serotypes simultaneously. To facilitate the development of a vaccine, good knowledge of different DENV serotype structures is crucial. Structures of DENV1 and DENV2 had been solved previously. Here we present a near-atomic resolution cryo-electron microscopy (cryo-EM) structure of mature DENV4. Comparison of the DENV4 structure with similar resolution cryo-EM structures of DENV1 and DENV2 showed differences in surface charge distribution, which may explain their differences in binding to cellular receptors such as heparin. Also, observed variations in amino acid residues involved in interactions between envelope and membrane proteins on the virus surface correlate with their ability to undergo structural changes at higher temperatures.

			Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus.,Kostyuchenko VA, Chew PL, Ng TS, Lok SM J Virol. 2013 Oct 23. PMID:24155405<ref>PMID:24155405</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4cbf" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 17:36, 7 September 2022

2022-09-07T17:36:40Z

← Older revision		Revision as of 17:36, 7 September 2022
Line 3:		Line 3:
	<SX load='4cbf' size='340' side='right' viewer='molstar' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>		<SX load='4cbf' size='340' side='right' viewer='molstar' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=4CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [https://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [https://pdbe.org/4cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [https://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [~~http~~://pdbe.org/4cbf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/E0WXI2_9FLAV E0WXI2_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS011998_004_099774]		[[https://www.uniprot.org/uniprot/E0WXI2_9FLAV E0WXI2_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS011998_004_099774]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Dengue virus (DENV), a mosquito-borne virus, is responsible for millions cases of infections worldwide. There are four DENV serotypes (DENV1 - 4). After a primary DENV infection, the antibodies elicited confer lifetime protection against that DENV serotype. However, in a secondary infection with another serotype, the pre-existing antibodies may cause antibody-dependent enhancement (ADE) of infection of macrophage cells leading to the development of the more severe form of disease, dengue hemorrhagic fever. Thus a safe vaccine should stimulate protection against all dengue serotypes simultaneously. To facilitate the development of a vaccine, good knowledge of different DENV serotype structures is crucial. Structures of DENV1 and DENV2 had been solved previously. Here we present a near-atomic resolution cryo-electron microscopy (cryo-EM) structure of mature DENV4. Comparison of the DENV4 structure with similar resolution cryo-EM structures of DENV1 and DENV2 showed differences in surface charge distribution, which may explain their differences in binding to cellular receptors such as heparin. Also, observed variations in amino acid residues involved in interactions between envelope and membrane proteins on the virus surface correlate with their ability to undergo structural changes at higher temperatures.

	~~Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus.,Kostyuchenko VA, Chew PL, Ng TS, Lok SM J Virol. 2013 Oct 23. PMID:24155405<ref>PMID:24155405</ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4cbf" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Dengue virus 2]]		[[Category: Dengue virus 2]]
			[[Category: Dengue virus 4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chew~~, P L~~]]		[[Category: Chew PL]]
	[[Category: Kostyuchenko~~, V A~~]]		[[Category: Kostyuchenko VA]]
	[[Category: Lok~~, S M~~]]		[[Category: Lok SM]]
	[[Category: Ng~~, T S]]~~		[[Category: Ng TS]]
	~~[[Category: Flavivirus]]~~
	~~[[Category: Virus~~]]

OCA at 19:00, 10 April 2020

2020-04-10T19:00:37Z

← Older revision		Revision as of 19:00, 10 April 2020
Line 3:		Line 3:
	<SX load='4cbf' size='340' side='right' viewer='molstar' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>		<SX load='4cbf' size='340' side='right' viewer='molstar' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://pdbe.org/4cbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://pdbe.org/4cbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 04:30, 5 March 2020

2020-03-05T04:30:48Z

← Older revision		Revision as of 04:30, 5 March 2020
Line 1:		Line 1:

	==Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus==		==Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus==
	<~~StructureSection~~ load='4cbf' size='340' side='right'caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>		<SX load='4cbf' size='340' side='right' viewer='molstar' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
Line 21:		Line 21:
	<references/>		<references/>
	__TOC__		__TOC__
	</~~StructureSection~~>		</SX>
	[[Category: Dengue virus 2]]		[[Category: Dengue virus 2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 16:42, 28 August 2019

2019-08-28T16:42:16Z

← Older revision		Revision as of 16:42, 28 August 2019
Line 1:		Line 1:

	==Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus==		==Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus==
	<StructureSection load='4cbf' size='340' side='right' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>		<StructureSection load='4cbf' size='340' side='right'caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Dengue virus 2]]		[[Category: Dengue virus 2]]
			[[Category: Large Structures]]
	[[Category: Chew, P L]]		[[Category: Chew, P L]]
	[[Category: Kostyuchenko, V A]]		[[Category: Kostyuchenko, V A]]

OCA at 22:21, 15 November 2017

2017-11-15T22:21:22Z

← Older revision		Revision as of 22:21, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4cbf' size='340' side='right' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>		<StructureSection load='4cbf' size='340' side='right' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Dengue_virus_4~~ Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://pdbe.org/4cbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://pdbe.org/4cbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Dengue virus 4]]		[[Category: Dengue virus 2]]
	[[Category: Chew, P L]]		[[Category: Chew, P L]]
	[[Category: Kostyuchenko, V A]]		[[Category: Kostyuchenko, V A]]

OCA at 00:13, 26 January 2017

2017-01-26T00:13:40Z

← Older revision		Revision as of 00:13, 26 January 2017
Line 1:		Line 1:

	==Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus==		==Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus==
	<StructureSection load='4cbf' size='340' side='right' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>		<StructureSection load='4cbf' size='340' side='right' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://pdbe.org/4cbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cbf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4cbf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:40, 22 April 2015

2015-04-22T06:40:48Z

← Older revision		Revision as of 06:40, 22 April 2015
Line 2:		Line 2:
	<StructureSection load='4cbf' size='340' side='right' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>		<StructureSection load='4cbf' size='340' side='right' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from ~~[http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and~~ [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Dengue virus 2]]~~
	[[Category: Dengue virus 4]]		[[Category: Dengue virus 4]]
	[[Category: Chew, P L]]		[[Category: Chew, P L]]

OCA at 20:39, 24 December 2014

2014-12-24T20:39:54Z

← Older revision		Revision as of 20:39, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/E0WXI2_9FLAV E0WXI2_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS011998_004_099774]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:30, 21 December 2014

2014-12-21T18:30:06Z

← Older revision		Revision as of 18:30, 21 December 2014
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	~~{{STRUCTURE_4cbf\| PDB=4cbf \| SCENE= }}~~		==Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus==
	===Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus===		<StructureSection load='4cbf' size='340' side='right' caption='[[4cbf]], [[Resolution\|resolution]] 4.10Å' scene=''>
	~~{{ABSTRACT_PUBMED_24155405}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4cbf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CBF FirstGlance]. <br>
			</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cau\|4cau]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cbf RCSB], [http://www.ebi.ac.uk/pdbsum/4cbf PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Dengue virus (DENV), a mosquito-borne virus, is responsible for millions cases of infections worldwide. There are four DENV serotypes (DENV1 - 4). After a primary DENV infection, the antibodies elicited confer lifetime protection against that DENV serotype. However, in a secondary infection with another serotype, the pre-existing antibodies may cause antibody-dependent enhancement (ADE) of infection of macrophage cells leading to the development of the more severe form of disease, dengue hemorrhagic fever. Thus a safe vaccine should stimulate protection against all dengue serotypes simultaneously. To facilitate the development of a vaccine, good knowledge of different DENV serotype structures is crucial. Structures of DENV1 and DENV2 had been solved previously. Here we present a near-atomic resolution cryo-electron microscopy (cryo-EM) structure of mature DENV4. Comparison of the DENV4 structure with similar resolution cryo-EM structures of DENV1 and DENV2 showed differences in surface charge distribution, which may explain their differences in binding to cellular receptors such as heparin. Also, observed variations in amino acid residues involved in interactions between envelope and membrane proteins on the virus surface correlate with their ability to undergo structural changes at higher temperatures.

	~~==Function==~~		Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus.,Kostyuchenko VA, Chew PL, Ng TS, Lok SM J Virol. 2013 Oct 23. PMID:24155405<ref>PMID:24155405</ref>
	~~[[http://www.uniprot.org/uniprot/E0WXI2_9FLAV E0WXI2_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by~~ virus ~~internalization through clathrin-mediated endocytosis~~. ~~Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes~~. ~~Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E~~. ~~After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS~~:~~SAAS011998_004_099774]~~

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[4cbf]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CBF OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:024155405</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Dengue virus 2]]		[[Category: Dengue virus 2]]
	[[Category: Dengue virus 4]]		[[Category: Dengue virus 4]]
	[[Category: Chew, P L.]]		[[Category: Chew, P L]]
	[[Category: Kostyuchenko, V A.]]		[[Category: Kostyuchenko, V A]]
	[[Category: Lok, S M.]]		[[Category: Lok, S M]]
	[[Category: Ng, T S.]]		[[Category: Ng, T S]]
	[[Category: Flavivirus]]		[[Category: Flavivirus]]
	[[Category: Virus]]		[[Category: Virus]]