4cot - Revision history

OCA at 12:13, 20 December 2023

2023-12-20T12:13:59Z

← Older revision		Revision as of 12:13, 20 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4COT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4COT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [https://pdbe.org/4cot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [https://www.ebi.ac.uk/pdbsum/4cot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cot ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [https://pdbe.org/4cot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [https://www.ebi.ac.uk/pdbsum/4cot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cot ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/EABN2_BACSU EABN2_BACSU]] Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of the alpha-1,5-L-arabinan to produce arabino-oligosaccharides and L-arabinose. It is also active toward linear branched sugar beet arabinan, and pectin from apple.<ref>PMID:18408032</ref> <ref>PMID:20883454</ref>		[https://www.uniprot.org/uniprot/EABN2_BACSU EABN2_BACSU] Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of the alpha-1,5-L-arabinan to produce arabino-oligosaccharides and L-arabinose. It is also active toward linear branched sugar beet arabinan, and pectin from apple.<ref>PMID:18408032</ref> <ref>PMID:20883454</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:25, 14 September 2022

2022-09-14T07:25:27Z

← Older revision		Revision as of 07:25, 14 September 2022
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	==The importance of the Abn2 calcium cluster in the endo-1,5- arabinanase activity from Bacillus subtilis==		==The importance of the Abn2 calcium cluster in the endo-1,5- arabinanase activity from Bacillus subtilis==
	<StructureSection load='4cot' size='340' side='right' caption='[[4cot]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4cot' size='340' side='right'caption='[[4cot]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4COT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4COT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] </span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [https://pdbe.org/4cot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [https://www.ebi.ac.uk/pdbsum/4cot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cot ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [~~http~~://pdbe.org/4cot PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cot PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cot ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/EABN2_BACSU EABN2_BACSU]] Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of the alpha-1,5-L-arabinan to produce arabino-oligosaccharides and L-arabinose. It is also active toward linear branched sugar beet arabinan, and pectin from apple.<ref>PMID:18408032</ref> <ref>PMID:20883454</ref>		[[https://www.uniprot.org/uniprot/EABN2_BACSU EABN2_BACSU]] Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of the alpha-1,5-L-arabinan to produce arabino-oligosaccharides and L-arabinose. It is also active toward linear branched sugar beet arabinan, and pectin from apple.<ref>PMID:18408032</ref> <ref>PMID:20883454</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Arabinanase\|Arabinanase]]		*[[Arabinanase 3D structures\|Arabinanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Arabinan endo-1,5-alpha-L-arabinosidase~~]]		[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: ~~Bacsu~~]]		[[Category: Large Structures]]
	[[Category: Bento, I]]		[[Category: Bento I]]
	[[Category: Carrondo~~, M A~~]]		[[Category: Carrondo MA]]
	[[Category: Correia, B]]		[[Category: Correia B]]
	[[Category: Ferreira~~, M J~~]]		[[Category: Ferreira MJ]]
	[[Category: Lahiri, S]]		[[Category: Lahiri S]]
	[[Category: Lindley~~, P F~~]]		[[Category: Lindley PF]]
	[[Category: McVey~~, C E~~]]		[[Category: McVey CE]]
	[[Category: ~~Sa-Nogueira, I de~~]]		[[Category: Soares CM]]
	[[Category: ~~Soares, C M~~]]		[[Category: De Sa-Nogueira I]]
	[[Category: DeSanctis, D]]		[[Category: DeSanctis D]]
	~~[[Category: Catalytic mechanism]]~~
	~~[[Category: Endo-alpha-l-arabinananase gh43]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Mutagenesis~~]]

OCA at 08:08, 6 March 2019

2019-03-06T08:08:56Z

← Older revision		Revision as of 08:08, 6 March 2019
Line 1:		Line 1:

	==The importance of the Abn2 calcium cluster in the endo-1,5- arabinanase activity from Bacillus subtilis==		==The importance of the Abn2 calcium cluster in the endo-1,5- arabinanase activity from Bacillus subtilis==
	<StructureSection load='4cot' size='340' side='right' caption='[[4cot]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4cot' size='340' side='right' caption='[[4cot]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4COT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4COT FirstGlance]. <br>

OCA at 12:05, 4 August 2016

2016-08-04T12:05:29Z

← Older revision		Revision as of 12:05, 4 August 2016
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	==The importance of the Abn2 calcium cluster in the endo-1,5- arabinanase activity from Bacillus subtilis==		==The importance of the Abn2 calcium cluster in the endo-1,5- arabinanase activity from Bacillus subtilis==
	<StructureSection load='4cot' size='340' side='right' caption='[[4cot]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4cot' size='340' side='right' caption='[[4cot]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [http://www.ebi.ac.uk/pdbsum/4cot PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [http://pdbe.org/4cot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [http://www.ebi.ac.uk/pdbsum/4cot PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cot ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4cot" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Arabinanase\|Arabinanase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:17, 24 December 2014

2014-12-24T14:17:13Z

← Older revision		Revision as of 14:17, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4COT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4COT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [http://www.ebi.ac.uk/pdbsum/4cot PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [http://www.ebi.ac.uk/pdbsum/4cot PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/EABN2_BACSU EABN2_BACSU]] Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of the alpha-1,5-L-arabinan to produce arabino-oligosaccharides and L-arabinose. It is also active toward linear branched sugar beet arabinan, and pectin from apple.<ref>PMID:18408032</ref> <ref>PMID:20883454</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis.,McVey CE, Ferreira MJ, Correia B, Lahiri S, de Sanctis D, Carrondo MA, Lindley PF, de Sa Nogueira I, Soares CM, Bento I J Biol Inorg Chem. 2014 Feb 19. PMID:24549757<ref>PMID:24549757</ref>		The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis.,McVey CE, Ferreira MJ, Correia B, Lahiri S, de Sanctis D, Carrondo MA, Lindley PF, de Sa Nogueira I, Soares CM, Bento I J Biol Inorg Chem. 2014 Feb 19. PMID:24549757<ref>PMID:24549757</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 23:
	[[Category: Arabinan endo-1,5-alpha-L-arabinosidase]]		[[Category: Arabinan endo-1,5-alpha-L-arabinosidase]]
	[[Category: Bacsu]]		[[Category: Bacsu]]
	[[Category: Bento, I.]]		[[Category: Bento, I]]
	[[Category: Carrondo, M A.]]		[[Category: Carrondo, M A]]
	[[Category: Correia, B.]]		[[Category: Correia, B]]
	[[Category: Ferreira, M J.]]		[[Category: Ferreira, M J]]
	[[Category: Lahiri, S.]]		[[Category: Lahiri, S]]
	[[Category: Lindley, P F.]]		[[Category: Lindley, P F]]
	[[Category: McVey, C E.]]		[[Category: McVey, C E]]
	[[Category: Sa-Nogueira, I de.]]		[[Category: Sa-Nogueira, I de]]
	[[Category: Soares, C M.]]		[[Category: Soares, C M]]
	[[Category: DeSanctis, D.]]		[[Category: DeSanctis, D]]
	[[Category: Catalytic mechanism]]		[[Category: Catalytic mechanism]]
	[[Category: Endo-alpha-l-arabinananase gh43]]		[[Category: Endo-alpha-l-arabinananase gh43]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Mutagenesis]]		[[Category: Mutagenesis]]

OCA at 12:03, 18 May 2014

2014-05-18T12:03:15Z

← Older revision		Revision as of 12:03, 18 May 2014
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	~~{{STRUCTURE_4cot\| PDB=4cot \| SCENE= }}~~		==The importance of the Abn2 calcium cluster in the endo-1,5- arabinanase activity from Bacillus subtilis==
	===The importance of the Abn2 calcium cluster in the endo-1,5- arabinanase activity from Bacillus subtilis===		<StructureSection load='4cot' size='340' side='right' caption='[[4cot]], [[Resolution\|resolution]] 1.90Å' scene=''>
	~~{{ABSTRACT_PUBMED_24549757}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4cot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4COT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4COT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cot RCSB], [http://www.ebi.ac.uk/pdbsum/4cot PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Arabinanase is a glycosyl hydrolase that is able to cleave the glycosidic bonds of alpha-1,5-L-arabinan, releasing arabino-oligosaccharides and L-arabinose. The enzyme has two domains, an N-terminal catalytic domain with a characteristic beta-propeller fold and a C-terminal domain whose function is unknown. A calcium ion, located near the catalytic site, serves to stabilize the N-terminal domain, but it has also been proposed to play a key role in the enzyme mechanism. The present work describes the structure of an inactive mutant of the wild-type enzyme (H318Q) and in which the calcium ion has been adventitiously replaced by nickel. These structural studies, together with functional and modelling studies, clearly support the role of the calcium ion in the overall reaction mechanism.

	~~==Function==~~		The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis.,McVey CE, Ferreira MJ, Correia B, Lahiri S, de Sanctis D, Carrondo MA, Lindley PF, de Sa Nogueira I, Soares CM, Bento I J Biol Inorg Chem. 2014 Feb 19. PMID:24549757<ref>PMID:24549757</ref>
	~~[[http://www.uniprot.org/uniprot/EABN2_BACSU EABN2_BACSU]] Involved in~~ the ~~degradation of arabinan and is a key enzyme~~ in the ~~complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of the alpha~~-1,5-~~L-arabinan to produce arabino-oligosaccharides and L-arabinose~~. ~~It is also active toward linear branched sugar beet arabinan~~, ~~and pectin from apple~~.~~<ref>~~PMID:~~18408032</ref>~~ <ref>PMID:~~20883454~~</ref>

	~~==About this Structure==~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
	~~[[4cot]] is~~ a ~~1 chain structure. Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4COT OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:024549757</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Arabinan endo-1,5-alpha-L-arabinosidase]]		[[Category: Arabinan endo-1,5-alpha-L-arabinosidase]]
			[[Category: Bacsu]]
	[[Category: Bento, I.]]		[[Category: Bento, I.]]
	[[Category: Carrondo, M A.]]		[[Category: Carrondo, M A.]]

OCA at 06:04, 26 March 2014

2014-03-26T06:04:14Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{STRUCTURE_4cot|  PDB=4cot  |  SCENE=  }}
-The entry 4cot is ON HOLD
+==Function==
-Authors: McVey, C.E., Ferreira, M.J., Correia, B., Lahiri, S., deSanctis, D., Carrondo, M.A., Lindley, P.F., de Sa-Nogueira, I., Soares, C.M., Bento, I.
+==About this Structure==
-Description: The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis
+==Reference==

OCA at 04:44, 13 February 2014

2014-02-13T04:44:38Z

← Older revision		Revision as of 04:44, 13 February 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4cot is ON HOLD ~~until sometime in the future~~		The entry 4cot is ON HOLD

	Authors: McVey, C.E., Ferreira, M.J., Correia, B., Lahiri, S., deSanctis, D., Carrondo, M.A., Lindley, P.F., de Sa-Nogueira, I., Soares, C.M., Bento, I.		Authors: McVey, C.E., Ferreira, M.J., Correia, B., Lahiri, S., deSanctis, D., Carrondo, M.A., Lindley, P.F., de Sa-Nogueira, I., Soares, C.M., Bento, I.

	Description: The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis		Description: The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis

OCA: Protected "4cot" [edit=sysop:move=sysop]

2014-02-05T09:53:05Z

Protected "4cot" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:53, 5 February 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4cot is ON HOLD until sometime in the future Authors: McVey, C.E., Ferreira, M.J., Correia, B., Lahiri, S., deSanctis, D., Carrondo, M.A., Lindley, ...

2014-02-05T09:53:04Z

New page: '''Unreleased structure''' The entry 4cot is ON HOLD until sometime in the future Authors: McVey, C.E., Ferreira, M.J., Correia, B., Lahiri, S., deSanctis, D., Carrondo, M.A., Lindley, ...

New page

'''Unreleased structure'''

The entry 4cot is ON HOLD until sometime in the future

Authors: McVey, C.E., Ferreira, M.J., Correia, B., Lahiri, S., deSanctis, D., Carrondo, M.A., Lindley, P.F., de Sa-Nogueira, I., Soares, C.M., Bento, I.

Description: The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis