4cs4 - Revision history

OCA at 11:13, 9 May 2024

2024-05-09T11:13:59Z

← Older revision		Revision as of 11:13, 9 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.349Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [https://pdbe.org/4cs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [https://www.ebi.ac.uk/pdbsum/4cs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cs4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [https://pdbe.org/4cs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [https://www.ebi.ac.uk/pdbsum/4cs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cs4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA]] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).		[https://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:30, 14 September 2022

2022-09-14T07:30:24Z

← Older revision		Revision as of 07:30, 14 September 2022
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	<StructureSection load='4cs4' size='340' side='right'caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='4cs4' size='340' side='right'caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dsm_2053 Dsm 2053~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cs2\|4cs2]], [[4cs3\|4cs3]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [https://pdbe.org/4cs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [https://www.ebi.ac.uk/pdbsum/4cs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cs4 ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [~~http~~://pdbe.org/4cs4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cs4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cs4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA]] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).		[[https://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA]] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Dsm 2053]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Pott, M]]		[[Category: Methanosarcina mazei]]
	[[Category: Schmidt~~, M J~~]]		[[Category: Pott M]]
	[[Category: Summerer, D]]		[[Category: Schmidt MJ]]
	[[Category: Weber, A]]		[[Category: Summerer D]]
	[[Category: Welte, W]]		[[Category: Weber A]]
	~~[[Category: Aminoacyl-trna synthetase]]~~		[[Category: Welte W]]
	~~[[Category: Ligase]]~~
	~~[[Category: Noncanonical amino acid~~]]

OCA at 08:54, 20 March 2019

2019-03-20T08:54:41Z

← Older revision		Revision as of 08:54, 20 March 2019
Line 1:		Line 1:

	==Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP==		==Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP==
	<StructureSection load='4cs4' size='340' side='right' caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='4cs4' size='340' side='right'caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2053 Dsm 2053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2053 Dsm 2053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 4cs4" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cs4" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Aminoacyl tRNA synthetase 3D structures\|Aminoacyl tRNA synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Dsm 2053]]		[[Category: Dsm 2053]]
			[[Category: Large Structures]]
	[[Category: Pott, M]]		[[Category: Pott, M]]
	[[Category: Schmidt, M J]]		[[Category: Schmidt, M J]]

OCA at 10:43, 5 August 2016

2016-08-05T10:43:20Z

← Older revision		Revision as of 10:43, 5 August 2016
Line 1:		Line 1:

	==Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP==		==Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP==
	<StructureSection load='4cs4' size='340' side='right' caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='4cs4' size='340' side='right' caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cs2\|4cs2]], [[4cs3\|4cs3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cs2\|4cs2]], [[4cs3\|4cs3]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://pdbe.org/4cs4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cs4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4cs4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:41, 25 December 2014

2014-12-25T01:41:53Z

← Older revision		Revision as of 01:41, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2053 Dsm 2053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2053 Dsm 2053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cs2\|4cs2]], [[4cs3\|4cs3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cs2\|4cs2]], [[4cs3\|4cs3]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA]] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Dsm 2053]]		[[Category: Dsm 2053]]
	[[Category: Pott, M.]]		[[Category: Pott, M]]
	[[Category: Schmidt, M J.]]		[[Category: Schmidt, M J]]
	[[Category: Summerer, D.]]		[[Category: Summerer, D]]
	[[Category: Weber, A.]]		[[Category: Weber, A]]
	[[Category: Welte, W.]]		[[Category: Welte, W]]
	[[Category: Aminoacyl-trna synthetase]]		[[Category: Aminoacyl-trna synthetase]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Noncanonical amino acid]]		[[Category: Noncanonical amino acid]]

OCA at 05:40, 27 August 2014

2014-08-27T05:40:14Z

← Older revision		Revision as of 05:40, 27 August 2014
Line 2:		Line 2:
	<StructureSection load='4cs4' size='340' side='right' caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='4cs4' size='340' side='right' caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. <br>		<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2053 Dsm 2053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CS4 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cs2\|4cs2]], [[4cs3\|4cs3]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cs2\|4cs2]], [[4cs3\|4cs3]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.~~2 2~~.7.1.2] </span></td></tr>		<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum]</span></td></tr>
	<table>		<table>
Line 14:		Line 14:
	Structural Basis of Furan-Amino Acid Recognition by a Polyspecific Aminoacyl-tRNA-Synthetase and its Genetic Encoding in Human Cells.,Schmidt MJ, Weber A, Pott M, Welte W, Summerer D Chembiochem. 2014 Apr 15. doi: 10.1002/cbic.201402006. PMID:24737732<ref>PMID:24737732</ref>		Structural Basis of Furan-Amino Acid Recognition by a Polyspecific Aminoacyl-tRNA-Synthetase and its Genetic Encoding in Human Cells.,Schmidt MJ, Weber A, Pott M, Welte W, Summerer D Chembiochem. 2014 Apr 15. doi: 10.1002/cbic.201402006. PMID:24737732<ref>PMID:24737732</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Dsm 2053]]
	[[Category: Pott, M.]]		[[Category: Pott, M.]]
	[[Category: Schmidt, M J.]]		[[Category: Schmidt, M J.]]

OCA at 09:24, 1 May 2014

2014-05-01T09:24:43Z

← Older revision		Revision as of 09:24, 1 May 2014
Line 2:		Line 2:
	<StructureSection load='4cs4' size='340' side='right' caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='4cs4' size='340' side='right' caption='[[4cs4]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	[[4cs4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. <br>		<table><tr><td colspan='2'>[[4cs4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. <br>
	<b>[[Ligand\|Ligands:]]</b> <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
	<b>[[Related_structure\|Related:]]</b> [[4cs2\|4cs2]], [[4cs3\|4cs3]]<br>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cs2\|4cs2]], [[4cs3\|4cs3]]</td></tr>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum]</span><br>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	The site-selective introduction of photo-crosslinking groups into proteins enables the discovery and mapping of weak and/or transient protein interactions with high spatiotemporal resolution, both in vitro and in vivo. We report the genetic encoding of a furan-based, photo-crosslinking amino acid in human cells; it can be activated with red light, thus offering high penetration depths in biological samples. This is achieved by activation of the amino acid and charging to its cognate tRNA by a pyrrolysyl-tRNA-synthetase (PylRS) mutant with broad polyspecificity. To gain insights into the recognition of this amino acid and to provide a rationale for its polyspecificity, we solved three crystal structures of the PylRS mutant: in its apo-form, in complex with adenosine 5'-(beta,gamma-imido)triphosphate (AMP-PNP) and in complex with the AMP ester of the furan amino acid. These structures provide clues for the observed polyspecificity and represent a promising starting point for the engineering of PylRS mutants with further increased substrate scope.		The site-selective introduction of photo-crosslinking groups into proteins enables the discovery and mapping of weak and/or transient protein interactions with high spatiotemporal resolution, both in vitro and in vivo. We report the genetic encoding of a furan-based, photo-crosslinking amino acid in human cells; it can be activated with red light, thus offering high penetration depths in biological samples. This is achieved by activation of the amino acid and charging to its cognate tRNA by a pyrrolysyl-tRNA-synthetase (PylRS) mutant with broad polyspecificity. To gain insights into the recognition of this amino acid and to provide a rationale for its polyspecificity, we solved three crystal structures of the PylRS mutant: in its apo-form, in complex with adenosine 5'-(beta,gamma-imido)triphosphate (AMP-PNP) and in complex with the AMP ester of the furan amino acid. These structures provide clues for the observed polyspecificity and represent a promising starting point for the engineering of PylRS mutants with further increased substrate scope.
Line 13:		Line 15:

	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:54, 30 April 2014

2014-04-30T09:54:01Z

← Older revision		Revision as of 09:54, 30 April 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	[[4cs4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. <br>		[[4cs4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS4 OCA]. <br>
	<b>Related:</b> [[4cs2\|4cs2]], [[4cs3\|4cs3]]<br>		<b>[[Ligand\|Ligands:]]</b> <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=AXZ:2-{[DIHYDROXY(4-AMINOETHYLPHENYL)-{4}-SULFANYL]AMINO}-3-HYDROXYPROPANOIC+ACID'>AXZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
			<b>[[Related_structure\|Related:]]</b> [[4cs2\|4cs2]], [[4cs3\|4cs3]]<br>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
			<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs4 PDBsum]</span><br>
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	The site-selective introduction of photo-crosslinking groups into proteins enables the discovery and mapping of weak and/or transient protein interactions with high spatiotemporal resolution, both in vitro and in vivo. We report the genetic encoding of a furan-based, photo-crosslinking amino acid in human cells; it can be activated with red light, thus offering high penetration depths in biological samples. This is achieved by activation of the amino acid and charging to its cognate tRNA by a pyrrolysyl-tRNA-synthetase (PylRS) mutant with broad polyspecificity. To gain insights into the recognition of this amino acid and to provide a rationale for its polyspecificity, we solved three crystal structures of the PylRS mutant: in its apo-form, in complex with adenosine 5'-(beta,gamma-imido)triphosphate (AMP-PNP) and in complex with the AMP ester of the furan amino acid. These structures provide clues for the observed polyspecificity and represent a promising starting point for the engineering of PylRS mutants with further increased substrate scope.		The site-selective introduction of photo-crosslinking groups into proteins enables the discovery and mapping of weak and/or transient protein interactions with high spatiotemporal resolution, both in vitro and in vivo. We report the genetic encoding of a furan-based, photo-crosslinking amino acid in human cells; it can be activated with red light, thus offering high penetration depths in biological samples. This is achieved by activation of the amino acid and charging to its cognate tRNA by a pyrrolysyl-tRNA-synthetase (PylRS) mutant with broad polyspecificity. To gain insights into the recognition of this amino acid and to provide a rationale for its polyspecificity, we solved three crystal structures of the PylRS mutant: in its apo-form, in complex with adenosine 5'-(beta,gamma-imido)triphosphate (AMP-PNP) and in complex with the AMP ester of the furan amino acid. These structures provide clues for the observed polyspecificity and represent a promising starting point for the engineering of PylRS mutants with further increased substrate scope.

OCA at 07:42, 30 April 2014

2014-04-30T07:42:46Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP==
-The entry 4cs4 is ON HOLD  until Paper Publication
+Structural Basis of Furan-Amino Acid Recognition by a Polyspecific Aminoacyl-tRNA-Synthetase and its Genetic Encoding in Human Cells.,Schmidt MJ, Weber A, Pott M, Welte W, Summerer D Chembiochem. 2014 Apr 15. doi: 10.1002/cbic.201402006. PMID:24737732<ref>PMID:24737732</ref>
-Authors: Schmidt, M.J., Weber, A., Pott, M., Welte, W., Summerer, D.
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+== References ==
-Description: Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP
+<references/>

OCA at 06:50, 19 March 2014

2014-03-19T06:50:41Z

← Older revision		Revision as of 06:50, 19 March 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4cs4 is ON HOLD		The entry 4cs4 is ON HOLD until Paper Publication

	Authors: Schmidt, M.J., Weber, A., Pott, M., Welte, W., Summerer, D.		Authors: Schmidt, M.J., Weber, A., Pott, M., Welte, W., Summerer, D.

	Description: Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP		Description: Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with AMPPNP