4dc2 - Revision history

OCA at 02:46, 21 November 2024

2024-11-21T02:46:39Z

← Older revision		Revision as of 02:46, 21 November 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dc2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dc2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DC2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc2 OCA], [https://pdbe.org/4dc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dc2 RCSB], [https://www.ebi.ac.uk/pdbsum/4dc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dc2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc2 OCA], [https://pdbe.org/4dc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dc2 RCSB], [https://www.ebi.ac.uk/pdbsum/4dc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dc2 ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	[[https://www.uniprot.org/uniprot/KPCI_MOUSE KPCI_MOUSE]] Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI3K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis (By similarity). Downstream of PI3K is required for insulin-stimulated glucose transport. Activates RAB4A and promotes its association with KIF3A which is required for the insulin-induced SLC2A4/GLUT4 translocation in adipocytes. Is essential in early embryogenesis and development of differentiating photoreceptors by playing a role in the establishment of epithelial and neuronal polarity.<ref>PMID:12832475</ref> <ref>PMID:14615604</ref> <ref>PMID:15322187</ref> <ref>PMID:16267237</ref> <ref>PMID:9971737</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:11, 21 September 2022

2022-09-21T08:11:00Z

← Older revision		Revision as of 08:11, 21 September 2022
Line 1:		Line 1:

	==Structure of PKC in Complex with a Substrate Peptide from Par-3==		==Structure of PKC in Complex with a Substrate Peptide from Par-3==
	<StructureSection load='4dc2' size='340' side='right' caption='[[4dc2]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4dc2' size='340' side='right'caption='[[4dc2]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dc2]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4DC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dc2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DC2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene~~></td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc2 OCA], [https://pdbe.org/4dc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dc2 RCSB], [https://www.ebi.ac.uk/pdbsum/4dc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dc2 ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pkcl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_kinase_C Protein kinase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.13 2.7.11.13] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4dc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc2 OCA], [~~http~~://pdbe.org/4dc2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4dc2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4dc2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4dc2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/KPCI_MOUSE KPCI_MOUSE]] Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI3K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis (By similarity). Downstream of PI3K is required for insulin-stimulated glucose transport. Activates RAB4A and promotes its association with KIF3A which is required for the insulin-induced SLC2A4/GLUT4 translocation in adipocytes. Is essential in early embryogenesis and development of differentiating photoreceptors by playing a role in the establishment of epithelial and neuronal polarity.<ref>PMID:12832475</ref> <ref>PMID:14615604</ref> <ref>PMID:15322187</ref> <ref>PMID:16267237</ref> <ref>PMID:9971737</ref> [[http://www.uniprot.org/uniprot/PARD3_RAT PARD3_RAT]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Targets the phosphatase PTEN to cell junctions.<ref>PMID:18082612</ref> <ref>PMID:18550519</ref>		[[https://www.uniprot.org/uniprot/KPCI_MOUSE KPCI_MOUSE]] Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI3K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis (By similarity). Downstream of PI3K is required for insulin-stimulated glucose transport. Activates RAB4A and promotes its association with KIF3A which is required for the insulin-induced SLC2A4/GLUT4 translocation in adipocytes. Is essential in early embryogenesis and development of differentiating photoreceptors by playing a role in the establishment of epithelial and neuronal polarity.<ref>PMID:12832475</ref> <ref>PMID:14615604</ref> <ref>PMID:15322187</ref> <ref>PMID:16267237</ref> <ref>PMID:9971737</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
	*[[Protein kinase C\|Protein kinase C]]		*[[Protein kinase C 3D structures\|Protein kinase C 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Large Structures]]
	[[Category: ~~Protein kinase C~~]]		[[Category: Mus musculus]]
	[[Category: Shang, Y]]		[[Category: Rattus norvegicus]]
	[[Category: Wang, C]]		[[Category: Shang Y]]
	[[Category: Yu, J]]		[[Category: Wang C]]
	[[Category: Zhang, M]]		[[Category: Yu J]]
	~~[[Category: Atypical pkc]]~~		[[Category: Zhang M]]
	~~[[Category: Cell polarity]]~~
	~~[[Category: Kinase]]~~
	~~[[Category: Par-3]]~~
	~~[[Category: Substrate]]~~
	~~[[Category: Transferase-transferase substrate complex~~]]

OCA at 00:32, 5 August 2016

2016-08-05T00:32:33Z

← Older revision		Revision as of 00:32, 5 August 2016
Line 1:		Line 1:

	==Structure of PKC in Complex with a Substrate Peptide from Par-3==		==Structure of PKC in Complex with a Substrate Peptide from Par-3==
	<StructureSection load='4dc2' size='340' side='right' caption='[[4dc2]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4dc2' size='340' side='right' caption='[[4dc2]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dc2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dc2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DC2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pkcl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pkcl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_kinase_C Protein kinase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.13 2.7.11.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_kinase_C Protein kinase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.13 2.7.11.13] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dc2 RCSB], [http://www.ebi.ac.uk/pdbsum/4dc2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc2 OCA], [http://pdbe.org/4dc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dc2 RCSB], [http://www.ebi.ac.uk/pdbsum/4dc2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dc2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PARD3_RAT PARD3_RAT]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Targets the phosphatase PTEN to cell junctions.<ref>PMID:18082612</ref> <ref>PMID:18550519</ref>		[[http://www.uniprot.org/uniprot/KPCI_MOUSE KPCI_MOUSE]] Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI3K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis (By similarity). Downstream of PI3K is required for insulin-stimulated glucose transport. Activates RAB4A and promotes its association with KIF3A which is required for the insulin-induced SLC2A4/GLUT4 translocation in adipocytes. Is essential in early embryogenesis and development of differentiating photoreceptors by playing a role in the establishment of epithelial and neuronal polarity.<ref>PMID:12832475</ref> <ref>PMID:14615604</ref> <ref>PMID:15322187</ref> <ref>PMID:16267237</ref> <ref>PMID:9971737</ref> [[http://www.uniprot.org/uniprot/PARD3_RAT PARD3_RAT]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Targets the phosphatase PTEN to cell junctions.<ref>PMID:18082612</ref> <ref>PMID:18550519</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4dc2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Protein kinase C]]		[[Category: Protein kinase C]]
	[[Category: Shang, Y]]		[[Category: Shang, Y]]

OCA at 14:23, 25 December 2014

2014-12-25T14:23:36Z

← Older revision		Revision as of 14:23, 25 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~4dc2~~.~~jpg~~\|~~left~~\|~~200px~~]]		==Structure of PKC in Complex with a Substrate Peptide from Par-3==
			<StructureSection load='4dc2' size='340' side='right' caption='[[4dc2]], [[Resolution\|resolution]] 2.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4dc2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DC2 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr>
			<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pkcl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_kinase_C Protein kinase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.13 2.7.11.13] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dc2 RCSB], [http://www.ebi.ac.uk/pdbsum/4dc2 PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PARD3_RAT PARD3_RAT]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Targets the phosphatase PTEN to cell junctions.<ref>PMID:18082612</ref> <ref>PMID:18550519</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Protein kinase C (PKC) play critical roles in many cellular functions including differentiation, proliferation, growth, and survival. However, the molecular bases governing PKC's substrate recognitions remain poorly understood. Here we determined the structure of PKCiota in complex with a peptide from Par-3 at 2.4 A. PKCiota in the complex adopts catalytically competent, closed conformation without phosphorylation of Thr402 in the activation loop. The Par-3 peptide binds to an elongated groove formed by the N- and C-lobes of the kinase domain. The PKCiota/Par-3 complex structure, together with extensive biochemical studies, reveals a set of substrate recognition sites common to all PKC isozymes as well as a hydrophobic pocket unique to aPKC. A consensus aPKC's substrate recognition sequence pattern can be readily identified based on the complex structure. Finally, we demonstrate that the pseudosubstrate sequence of PKCiota resembles its substrate sequence, directly binds to and inhibits the activity of the kinase.

	~~{{STRUCTURE_4dc2\| PDB=4dc2 \| SCENE= }}~~		Substrate recognition mechanism of atypical protein kinase Cs revealed by the structure of PKCiota in complex with a substrate peptide from Par-3.,Wang C, Shang Y, Yu J, Zhang M Structure. 2012 May 9;20(5):791-801. PMID:22579248<ref>PMID:22579248</ref>

	~~===Structure~~ of ~~PKC in Complex with a Substrate Peptide from Par-3===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22579248}}~~		==See Also==
			*[[Protein kinase C\|Protein kinase C]]
	==~~About this Structure~~==		== References ==
	[[~~4dc2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC2 OCA~~].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:022579248<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Protein kinase C]]		[[Category: Protein kinase C]]
	[[Category: Shang, Y.]]		[[Category: Shang, Y]]
	[[Category: Wang, C.]]		[[Category: Wang, C]]
	[[Category: Yu, J.]]		[[Category: Yu, J]]
	[[Category: Zhang, M.]]		[[Category: Zhang, M]]
	[[Category: Atypical pkc]]		[[Category: Atypical pkc]]
	[[Category: Cell polarity]]		[[Category: Cell polarity]]

OCA at 08:08, 11 July 2012

2012-07-11T08:08:45Z

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-'''Unreleased structure'''
+[[Image:4dc2.jpg|left|200px]]
-The entry 4dc2 is ON HOLD  until Paper Publication
+{{STRUCTURE_4dc2|  PDB=4dc2  |  SCENE=  }}
-Authors: Shang, Y., Wang, C., Yu, J., Zhang, M.
+===Structure of PKC in Complex with a Substrate Peptide from Par-3===
-Description: Structure of PKC in Complex with a Substrate Peptide from Par-3
+{{ABSTRACT_PUBMED_22579248}}

OCA at 05:40, 13 June 2012

2012-06-13T05:40:15Z

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	The entry 4dc2 is ON HOLD		The entry 4dc2 is ON HOLD until Paper Publication

	Authors: Shang, Y., Wang, C., Yu, J., Zhang, M.		Authors: Shang, Y., Wang, C., Yu, J., Zhang, M.

	Description: Structure of PKC in Complex with a Substrate Peptide from Par-3		Description: Structure of PKC in Complex with a Substrate Peptide from Par-3

OCA at 06:59, 1 February 2012

2012-02-01T06:59:56Z

← Older revision		Revision as of 06:59, 1 February 2012
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	Authors: ~~Yuan~~,~~SHANG~~, ~~Chihao~~,~~WANG~~, ~~Jiang~~,YU, ~~Mingjie~~,~~ZHANG~~		Authors: Shang, Y., Wang, C., Yu, J., Zhang, M.

	Description: Structure of PKC in Complex with a Substrate Peptide from Par-3		Description: Structure of PKC in Complex with a Substrate Peptide from Par-3

OCA: Protected "4dc2" [edit=sysop:move=sysop]

2012-01-25T06:11:10Z

Protected "4dc2" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:11, 25 January 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4dc2 is ON HOLD Authors: Yuan,SHANG, Chihao,WANG, Jiang,YU, Mingjie,ZHANG Description: Structure of PKC in Complex with a Substrate Peptide from Pa...

2012-01-25T06:11:08Z

New page: '''Unreleased structure''' The entry 4dc2 is ON HOLD Authors: Yuan,SHANG, Chihao,WANG, Jiang,YU, Mingjie,ZHANG Description: Structure of PKC in Complex with a Substrate Peptide from Pa...

New page

'''Unreleased structure'''

The entry 4dc2 is ON HOLD

Authors: Yuan,SHANG, Chihao,WANG, Jiang,YU, Mingjie,ZHANG

Description: Structure of PKC in Complex with a Substrate Peptide from Par-3