4e0f - Revision history

OCA at 14:49, 14 March 2024

2024-03-14T14:49:07Z

← Older revision		Revision as of 14:49, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E0F FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E0F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.85Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [https://pdbe.org/4e0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [https://www.ebi.ac.uk/pdbsum/4e0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e0f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [https://pdbe.org/4e0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [https://www.ebi.ac.uk/pdbsum/4e0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e0f ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Riboflavin synthase (RS) catalyzes the last step of riboflavin biosynthesis in microorganisms and plants, which corresponds to the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine to yield one molecule of riboflavin and one molecule of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Owing to the absence of this enzyme in animals and the fact that most pathogenic bacteria show a strict dependence on riboflavin biosynthesis, RS has been proposed as a potential target for antimicrobial drug development. Eubacterial, fungal and plant RSs assemble as homotrimers lacking C3 symmetry. Each monomer can bind two substrate molecules, yet there is only one active site for the whole enzyme, which is located at the interface between two neighbouring chains. This work reports the crystallographic structure of RS from the pathogenic bacterium Brucella abortus (the aetiological agent of the disease brucellosis) in its apo form, in complex with riboflavin and in complex with two different product analogues, being the first time that the structure of an intact RS trimer with bound ligands has been solved. These crystal models support the hypothesis of enhanced flexibility in the particle and also highlight the role of the ligands in assembling the unique active site. Kinetic and binding studies were also performed to complement these findings. The structural and biochemical information generated may be useful for the rational design of novel RS inhibitors with antimicrobial activity.

	Crystallographic and kinetic study of riboflavin synthase from Brucella abortus, a chemotherapeutic target with an enhanced intrinsic flexibility.,Serer MI, Bonomi HR, Guimaraes BG, Rossi RC, Goldbaum FA, Klinke S Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1419-34. doi:, 10.1107/S1399004714005161. Epub 2014 Apr 30. PMID:24816110<ref>PMID:24816110</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e0f" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:43, 28 September 2022

2022-09-28T06:43:11Z

← Older revision		Revision as of 06:43, 28 September 2022
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	==Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin==		==Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin==
	<StructureSection load='4e0f' size='340' side='right' caption='[[4e0f]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='4e0f' size='340' side='right'caption='[[4e0f]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E0F FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E0F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i8d\|1i8d]], [[1kzl\|1kzl]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [https://pdbe.org/4e0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [https://www.ebi.ac.uk/pdbsum/4e0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e0f ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ribE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [~~http~~://pdbe.org/4e0f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e0f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e0f ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus of abortion bang 1897~~]]		[[Category: Brucella abortus]]
	[[Category: ~~Riboflavin synthase~~]]		[[Category: Large Structures]]
	[[Category: Bonomi~~, H R~~]]		[[Category: Bonomi HR]]
	[[Category: Goldbaum~~, F A~~]]		[[Category: Goldbaum FA]]
	[[Category: Guimaraes~~, B G~~]]		[[Category: Guimaraes BG]]
	[[Category: Klinke, S]]		[[Category: Klinke S]]
	[[Category: Rossi~~, R C~~]]		[[Category: Rossi RC]]
	[[Category: Serer~~, M I]]~~		[[Category: Serer MI]]
	~~[[Category: Alpha + beta protein]]~~
	~~[[Category: Beta barrel]]~~
	~~[[Category: Riboflavin biosynthesis]]~~
	~~[[Category: Transferase~~]]

OCA at 09:37, 11 August 2016

2016-08-11T09:37:40Z

← Older revision		Revision as of 09:37, 11 August 2016
Line 1:		Line 1:

	==Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin==		==Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin==
	<StructureSection load='4e0f' size='340' side='right' caption='[[4e0f]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='4e0f' size='340' side='right' caption='[[4e0f]], [[Resolution\|resolution]] 2.85Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ribE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ribE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://pdbe.org/4e0f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e0f ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e0f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:13, 4 January 2015

2015-01-04T13:13:02Z

← Older revision		Revision as of 13:13, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E0F FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E0F FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i8d\|1i8d]], [[1kzl\|1kzl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i8d\|1i8d]], [[1kzl\|1kzl]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ribE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ribE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	Crystallographic and kinetic study of riboflavin synthase from Brucella abortus, a chemotherapeutic target with an enhanced intrinsic flexibility.,Serer MI, Bonomi HR, Guimaraes BG, Rossi RC, Goldbaum FA, Klinke S Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1419-34. doi:, 10.1107/S1399004714005161. Epub 2014 Apr 30. PMID:24816110<ref>PMID:24816110</ref>		Crystallographic and kinetic study of riboflavin synthase from Brucella abortus, a chemotherapeutic target with an enhanced intrinsic flexibility.,Serer MI, Bonomi HR, Guimaraes BG, Rossi RC, Goldbaum FA, Klinke S Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1419-34. doi:, 10.1107/S1399004714005161. Epub 2014 Apr 30. PMID:24816110<ref>PMID:24816110</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 23:		Line 23:
	[[Category: Bacillus of abortion bang 1897]]		[[Category: Bacillus of abortion bang 1897]]
	[[Category: Riboflavin synthase]]		[[Category: Riboflavin synthase]]
	[[Category: Bonomi, H R.]]		[[Category: Bonomi, H R]]
	[[Category: Goldbaum, F A.]]		[[Category: Goldbaum, F A]]
	[[Category: Guimaraes, B G.]]		[[Category: Guimaraes, B G]]
	[[Category: Klinke, S.]]		[[Category: Klinke, S]]
	[[Category: Rossi, R C.]]		[[Category: Rossi, R C]]
	[[Category: Serer, M I.]]		[[Category: Serer, M I]]
	[[Category: Alpha + beta protein]]		[[Category: Alpha + beta protein]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Riboflavin biosynthesis]]		[[Category: Riboflavin biosynthesis]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 06:54, 28 May 2014

2014-05-28T06:54:12Z

← Older revision		Revision as of 06:54, 28 May 2014
Line 2:		Line 2:
	<StructureSection load='4e0f' size='340' side='right' caption='[[4e0f]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='4e0f' size='340' side='right' caption='[[4e0f]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. <br>		<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E0F FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene><br>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i8d\|1i8d]], [[1kzl\|1kzl]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i8d\|1i8d]], [[1kzl\|1kzl]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ribE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr>		<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ribE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>		<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum]</span></td></tr>
	<table>		<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Riboflavin synthase (RS) catalyzes the last step of riboflavin biosynthesis in microorganisms and plants, which corresponds to the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine to yield one molecule of riboflavin and one molecule of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Owing to the absence of this enzyme in animals and the fact that most pathogenic bacteria show a strict dependence on riboflavin biosynthesis, RS has been proposed as a potential target for antimicrobial drug development. Eubacterial, fungal and plant RSs assemble as homotrimers lacking C3 symmetry. Each monomer can bind two substrate molecules, yet there is only one active site for the whole enzyme, which is located at the interface between two neighbouring chains. This work reports the crystallographic structure of RS from the pathogenic bacterium Brucella abortus (the aetiological agent of the disease brucellosis) in its apo form, in complex with riboflavin and in complex with two different product analogues, being the first time that the structure of an intact RS trimer with bound ligands has been solved. These crystal models support the hypothesis of enhanced flexibility in the particle and also highlight the role of the ligands in assembling the unique active site. Kinetic and binding studies were also performed to complement these findings. The structural and biochemical information generated may be useful for the rational design of novel RS inhibitors with antimicrobial activity.

			Crystallographic and kinetic study of riboflavin synthase from Brucella abortus, a chemotherapeutic target with an enhanced intrinsic flexibility.,Serer MI, Bonomi HR, Guimaraes BG, Rossi RC, Goldbaum FA, Klinke S Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1419-34. doi:, 10.1107/S1399004714005161. Epub 2014 Apr 30. PMID:24816110<ref>PMID:24816110</ref>

			From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:32, 14 May 2014

2014-05-14T07:32:20Z

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	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum]</span></td></tr>
	<table>		<table>
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:38, 14 May 2014

2014-05-14T06:38:56Z

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	~~{{STRUCTURE_4e0f\| PDB=4e0f \| SCENE= }}~~		==Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin==
	===Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin===		<StructureSection load='4e0f' size='340' side='right' caption='[[4e0f]], [[Resolution\|resolution]] 2.85Å' scene=''>
			== Structural highlights ==
	==~~About this Structure~~==		<table><tr><td colspan='2'>[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA]. <br>
	[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA].		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i8d\|1i8d]], [[1kzl\|1kzl]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ribE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e0f RCSB], [http://www.ebi.ac.uk/pdbsum/4e0f PDBsum]</span></td></tr>
			<table>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Bacillus of abortion bang 1897]]		[[Category: Bacillus of abortion bang 1897]]
	[[Category: Riboflavin synthase]]		[[Category: Riboflavin synthase]]

OCA at 14:23, 12 March 2014

2014-03-12T14:23:12Z

← Older revision		Revision as of 14:23, 12 March 2014
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	==About this Structure==		==About this Structure==
	[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Brucella_abortus Brucella abortus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA].		[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA].
	[[Category: ~~Brucella abortus~~]]		[[Category: Bacillus of abortion bang 1897]]
	[[Category: Riboflavin synthase]]		[[Category: Riboflavin synthase]]
			[[Category: Bonomi, H R.]]
	[[Category: Goldbaum, F A.]]		[[Category: Goldbaum, F A.]]
			[[Category: Guimaraes, B G.]]
	[[Category: Klinke, S.]]		[[Category: Klinke, S.]]
			[[Category: Rossi, R C.]]
	[[Category: Serer, M I.]]		[[Category: Serer, M I.]]
	[[Category: Alpha + beta protein]]		[[Category: Alpha + beta protein]]

OCA at 08:06, 16 October 2013

2013-10-16T08:06:43Z

← Older revision		Revision as of 08:06, 16 October 2013
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	~~'''Unreleased~~ structure~~'''~~		{{STRUCTURE_4e0f\| PDB=4e0f \| SCENE= }}
			===Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin===

	~~The entry~~ 4e0f is ~~ON HOLD until Paper Publication~~		==About this Structure==
			[[4e0f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E0F OCA].
	~~Authors~~: Klinke, S., Serer, M.I.~~, Goldbaum, F.A.~~		[[Category: Brucella abortus]]
			[[Category: Riboflavin synthase]]
	~~Description~~: ~~Crystallographic structure of trimeric~~ Riboflavin ~~Synthase from Brucella abortus in complex with riboflavin~~		[[Category: Goldbaum, F A.]]
			[[Category: Klinke, S.]]
			[[Category: Serer, M I.]]
			[[Category: Alpha + beta protein]]
			[[Category: Beta barrel]]
			[[Category: Riboflavin biosynthesis]]
			[[Category: Transferase]]

OCA at 07:14, 21 March 2012

2012-03-21T07:14:11Z

← Older revision		Revision as of 07:14, 21 March 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4e0f is ON HOLD		The entry 4e0f is ON HOLD until Paper Publication

	Authors: Klinke, S., Serer, M.I., Goldbaum, F.A.		Authors: Klinke, S., Serer, M.I., Goldbaum, F.A.

	Description: Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin		Description: Crystallographic structure of trimeric Riboflavin Synthase from Brucella abortus in complex with riboflavin