4e1f - Revision history

OCA at 14:49, 14 March 2024

2024-03-14T14:49:47Z

← Older revision		Revision as of 14:49, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1F FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1f OCA], [https://pdbe.org/4e1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1f RCSB], [https://www.ebi.ac.uk/pdbsum/4e1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1f OCA], [https://pdbe.org/4e1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1f RCSB], [https://www.ebi.ac.uk/pdbsum/4e1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/VGRG1_VIBC3 VGRG1_VIBC3]] Part of the type VI secretion system (T6SS) specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (By similarity). Forms the spike at the tip of the elongating tube probably formed by hemolysin co-regulated protein/Hcp. Allows the delivery of the TseL antibacterial toxin to target cells where it exerts its toxicity (By similarity). Acts also directly as an actin-directed toxin that catalyzes the covalent cross-linking of host cytoplasmic monomeric actin. Mediates the cross-link between 'Lys-50' of one monomer and 'Glu-270' of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:22898822). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:22898822). Acts as an acid--amino-acid ligase that transfers the gamma-phosphoryl group of ATP to the 'Glu-270' actin residue, resulting in the formation of an activated acyl phosphate intermediate. This intermediate is further hydrolyzed and the energy of hydrolysis is utilized for the formation of the amide bond between actin subunits (PubMed:22898822).[UniProtKB:Q9KS45]<ref>PMID:22898822</ref>		[https://www.uniprot.org/uniprot/VGRG1_VIBC3 VGRG1_VIBC3] Part of the type VI secretion system (T6SS) specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (By similarity). Forms the spike at the tip of the elongating tube probably formed by hemolysin co-regulated protein/Hcp. Allows the delivery of the TseL antibacterial toxin to target cells where it exerts its toxicity (By similarity). Acts also directly as an actin-directed toxin that catalyzes the covalent cross-linking of host cytoplasmic monomeric actin. Mediates the cross-link between 'Lys-50' of one monomer and 'Glu-270' of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:22898822). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:22898822). Acts as an acid--amino-acid ligase that transfers the gamma-phosphoryl group of ATP to the 'Glu-270' actin residue, resulting in the formation of an activated acyl phosphate intermediate. This intermediate is further hydrolyzed and the energy of hydrolysis is utilized for the formation of the amide bond between actin subunits (PubMed:22898822).[UniProtKB:Q9KS45]<ref>PMID:22898822</ref>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:44, 28 September 2022

2022-09-28T06:44:51Z

← Older revision		Revision as of 06:44, 28 September 2022
Line 1:		Line 1:

	==Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++==		==Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++==
	<StructureSection load='4e1f' size='340' side='right' caption='[[4e1f]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4e1f' size='340' side='right'caption='[[4e1f]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1f]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E1F FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1d\|4e1d]], [[4dtl\|4dtl]], [[4e1c\|4e1c]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1f OCA], [https://pdbe.org/4e1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1f RCSB], [https://www.ebi.ac.uk/pdbsum/4e1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1f ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC_1416, VgrG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1f OCA], [~~http~~://pdbe.org/4e1f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e1f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e1f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1f ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/VGRG1_VIBC3 VGRG1_VIBC3]] Part of the type VI secretion system (T6SS) specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (By similarity). Forms the spike at the tip of the elongating tube probably formed by hemolysin co-regulated protein/Hcp. Allows the delivery of the TseL antibacterial toxin to target cells where it exerts its toxicity (By similarity). Acts also directly as an actin-directed toxin that catalyzes the covalent cross-linking of host cytoplasmic monomeric actin. Mediates the cross-link between 'Lys-50' of one monomer and 'Glu-270' of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:22898822). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:22898822). Acts as an acid--amino-acid ligase that transfers the gamma-phosphoryl group of ATP to the 'Glu-270' actin residue, resulting in the formation of an activated acyl phosphate intermediate. This intermediate is further hydrolyzed and the energy of hydrolysis is utilized for the formation of the amide bond between actin subunits (PubMed:22898822).[UniProtKB:Q9KS45]<ref>PMID:22898822</ref>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillo virgola del koch trevisan 1884~~]]		[[Category: Large Structures]]
	[[Category: Audoly, G]]		[[Category: Vibrio cholerae]]
	[[Category: Cambillau, C]]		[[Category: Audoly G]]
	[[Category: Cascales, E]]		[[Category: Cambillau C]]
	[[Category: Derrez, E]]		[[Category: Cascales E]]
	[[Category: Durand, E]]		[[Category: Derrez E]]
	[[Category: Ortiz-Lombardia, M]]		[[Category: Durand E]]
	[[Category: Raoult, D]]		[[Category: Ortiz-Lombardia M]]
	[[Category: Spinelli, S]]		[[Category: Raoult D]]
	~~[[Category: Alpha/beta protein]]~~		[[Category: Spinelli S]]
	~~[[Category: G-actin cross-linking toxin]]~~
	~~[[Category: Toxin~~]]

OCA at 07:57, 11 August 2016

2016-08-11T07:57:56Z

← Older revision		Revision as of 07:57, 11 August 2016
Line 1:		Line 1:

	==Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++==		==Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++==
	<StructureSection load='4e1f' size='340' side='right' caption='[[4e1f]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4e1f' size='340' side='right' caption='[[4e1f]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1d\|4e1d]], [[4dtl\|4dtl]], [[4e1c\|4e1c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1d\|4e1d]], [[4dtl\|4dtl]], [[4e1c\|4e1c]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC_1416, VgrG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC_1416, VgrG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1f RCSB], [http://www.ebi.ac.uk/pdbsum/4e1f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1f OCA], [http://pdbe.org/4e1f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e1f RCSB], [http://www.ebi.ac.uk/pdbsum/4e1f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1f ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 13:17, 4 January 2015

2015-01-04T13:17:59Z

← Older revision		Revision as of 13:17, 4 January 2015
Line 1:		Line 1:
	~~[[Image:4e1f.jpg\|left\|200px]]~~		==Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++==
			<StructureSection load='4e1f' size='340' side='right' caption='[[4e1f]], [[Resolution\|resolution]] 2.10Å' scene=''>
	~~{{STRUCTURE_4e1f\| PDB=4e1f \| SCENE= }}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4e1f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1F FirstGlance]. <br>
	===Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++===		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1d\|4e1d]], [[4dtl\|4dtl]], [[4e1c\|4e1c]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC_1416, VgrG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
	==~~About this Structure~~==		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1f RCSB], [http://www.ebi.ac.uk/pdbsum/4e1f PDBsum]</span></td></tr>
	[[4e1f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1F OCA].		</table>
	[[Category: Audoly, G.]]		__TOC__
	[[Category: Cambillau, C.]]		</StructureSection>
	[[Category: Cascales, E.]]		[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Derrez, E.]]		[[Category: Audoly, G]]
	[[Category: Durand, E.]]		[[Category: Cambillau, C]]
	[[Category: Ortiz-Lombardia, M.]]		[[Category: Cascales, E]]
	[[Category: Raoult, D.]]		[[Category: Derrez, E]]
	[[Category: Spinelli, S.]]		[[Category: Durand, E]]
			[[Category: Ortiz-Lombardia, M]]
			[[Category: Raoult, D]]
			[[Category: Spinelli, S]]
	[[Category: Alpha/beta protein]]		[[Category: Alpha/beta protein]]
	[[Category: G-actin cross-linking toxin]]		[[Category: G-actin cross-linking toxin]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 07:27, 10 October 2012

2012-10-10T07:27:07Z

← Older revision		Revision as of 07:27, 10 October 2012
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4e1f.jpg\|left\|200px]]

	~~The entry~~ 4e1f ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_4e1f\| PDB=4e1f \| SCENE= }}

	~~Authors: Durand, E., Audoly, G., Derrez, E., Spinelli, S., Ortiz-Lombardia, M., Cascales, E., Raoult, D., Cambillau, C.~~		===Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++===

	~~Description:~~ Structure of a ~~VgrG Vibrio cholerae~~ toxin ~~ACD domain Glu16Gln mutant in complex with ADP and Mn++~~
			==About this Structure==
			[[4e1f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1F OCA].
			[[Category: Audoly, G.]]
			[[Category: Cambillau, C.]]
			[[Category: Cascales, E.]]
			[[Category: Derrez, E.]]
			[[Category: Durand, E.]]
			[[Category: Ortiz-Lombardia, M.]]
			[[Category: Raoult, D.]]
			[[Category: Spinelli, S.]]
			[[Category: Alpha/beta protein]]
			[[Category: G-actin cross-linking toxin]]
			[[Category: Toxin]]

OCA: Protected "4e1f" [edit=sysop:move=sysop]

2012-03-14T06:15:56Z

Protected "4e1f" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:15, 14 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e1f is ON HOLD until Paper Publication Authors: Durand, E., Audoly, G., Derrez, E., Spinelli, S., Ortiz-Lombardia, M., Cascales, E., Raoult, D., Ca...

2012-03-14T06:15:55Z

New page: '''Unreleased structure''' The entry 4e1f is ON HOLD until Paper Publication Authors: Durand, E., Audoly, G., Derrez, E., Spinelli, S., Ortiz-Lombardia, M., Cascales, E., Raoult, D., Ca...

New page

'''Unreleased structure'''

The entry 4e1f is ON HOLD until Paper Publication

Authors: Durand, E., Audoly, G., Derrez, E., Spinelli, S., Ortiz-Lombardia, M., Cascales, E., Raoult, D., Cambillau, C.

Description: Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++