4e1s - Revision history

OCA at 11:01, 1 March 2024

2024-03-01T11:01:18Z

← Older revision		Revision as of 11:01, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1S FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.855Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [https://pdbe.org/4e1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [https://www.ebi.ac.uk/pdbsum/4e1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [https://pdbe.org/4e1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [https://www.ebi.ac.uk/pdbsum/4e1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57]] Necessary for the production of attaching and effacing lesions on tissue culture cells.		[https://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57] Necessary for the production of attaching and effacing lesions on tissue culture cells.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Intimins and invasins are virulence factors produced by pathogenic Gram-negative bacteria. They contain C-terminal extracellular passenger domains that are involved in adhesion to host cells and N-terminal beta domains that are embedded in the outer membrane. Here, we identify the domain boundaries of an E. coli intimin beta domain and use this information to solve its structure and the beta domain structure of a Y. pseudotuberculosis invasin. Both beta domain structures crystallized as monomers and reveal that the previous range of residues assigned to the beta domain also includes a protease-resistant domain that is part of the passenger. Additionally, we identify 146 nonredundant representative members of the intimin/invasin family based on the boundaries of the highly conserved intimin and invasin beta domains. We then use this set of sequences along with our structural data to find and map the evolutionarily constrained residues within the beta domain.

	Crystal Structures of the Outer Membrane Domain of Intimin and Invasin from Enterohemorrhagic E. coli and Enteropathogenic Y. pseudotuberculosis.,Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK Structure. 2012 May 31. PMID:22658748<ref>PMID:22658748</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e1s" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:45, 28 September 2022

2022-09-28T06:45:21Z

← Older revision		Revision as of 06:45, 28 September 2022
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	==X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7==		==X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7==
	<StructureSection load='4e1s' size='340' side='right' caption='[[4e1s]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='4e1s' size='340' side='right'caption='[[4e1s]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E1S FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1t\|4e1t]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [https://pdbe.org/4e1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [https://www.ebi.ac.uk/pdbsum/4e1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1s ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eae, eaeA, ECs4559, Intimin, L0025, Z5110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [~~http~~://pdbe.org/4e1s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e1s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57]] Necessary for the production of attaching and effacing lesions on tissue culture cells.		[[https://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57]] Necessary for the production of attaching and effacing lesions on tissue culture cells.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Eco57~~]]		[[Category: Escherichia coli O157:H7]]
	[[Category: Barnard~~, T J~~]]		[[Category: Large Structures]]
	[[Category: Buchanan~~, S K~~]]		[[Category: Barnard TJ]]
	[[Category: Cherezov, V]]		[[Category: Buchanan SK]]
	[[Category: Dautin, N]]		[[Category: Cherezov V]]
	[[Category: Fairman~~, J W~~]]		[[Category: Dautin N]]
	[[Category: Finkelstein, A]]		[[Category: Fairman JW]]
	[[Category: Noinaj, N]]		[[Category: Finkelstein A]]
	[[Category: Przytycka~~, T M~~]]		[[Category: Noinaj N]]
	[[Category: Udho, E]]		[[Category: Przytycka TM]]
	[[Category: Wei, L]]		[[Category: Udho E]]
	[[Category: Wojtowicz, D]]		[[Category: Wei L]]
	~~[[Category: Adhesin]]~~		[[Category: Wojtowicz D]]
	~~[[Category: Cell adhesion]]~~
	~~[[Category: Outer membrane beta barrel]]~~
	~~[[Category: Translocated intimin receptor~~]]

OCA at 21:58, 4 August 2016

2016-08-04T21:58:45Z

← Older revision		Revision as of 21:58, 4 August 2016
Line 1:		Line 1:

	==X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7==		==X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7==
	<StructureSection load='4e1s' size='340' side='right' caption='[[4e1s]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='4e1s' size='340' side='right' caption='[[4e1s]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1S FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1t\|4e1t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1t\|4e1t]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eae, eaeA, ECs4559, Intimin, L0025, Z5110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eae, eaeA, ECs4559, Intimin, L0025, Z5110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [http://www.ebi.ac.uk/pdbsum/4e1s PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [http://pdbe.org/4e1s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [http://www.ebi.ac.uk/pdbsum/4e1s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e1s" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Escherichia coli~~]]		[[Category: Eco57]]
	[[Category: Barnard, T J]]		[[Category: Barnard, T J]]
	[[Category: Buchanan, S K]]		[[Category: Buchanan, S K]]

OCA at 13:29, 25 December 2014

2014-12-25T13:29:19Z

← Older revision		Revision as of 13:29, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1S FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1S FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1t\|4e1t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1t\|4e1t]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eae, eaeA, ECs4559, Intimin, L0025, Z5110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eae, eaeA, ECs4559, Intimin, L0025, Z5110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [http://www.ebi.ac.uk/pdbsum/4e1s PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [http://www.ebi.ac.uk/pdbsum/4e1s PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57]] Necessary for the production of attaching and effacing lesions on tissue culture cells.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Barnard, T J.]]		[[Category: Barnard, T J]]
	[[Category: Buchanan, S K.]]		[[Category: Buchanan, S K]]
	[[Category: Cherezov, V.]]		[[Category: Cherezov, V]]
	[[Category: Dautin, N.]]		[[Category: Dautin, N]]
	[[Category: Fairman, J W.]]		[[Category: Fairman, J W]]
	[[Category: Finkelstein, A.]]		[[Category: Finkelstein, A]]
	[[Category: Noinaj, N.]]		[[Category: Noinaj, N]]
	[[Category: Przytycka, T M.]]		[[Category: Przytycka, T M]]
	[[Category: Udho, E.]]		[[Category: Udho, E]]
	[[Category: Wei, L.]]		[[Category: Wei, L]]
	[[Category: Wojtowicz, D.]]		[[Category: Wojtowicz, D]]
	[[Category: Adhesin]]		[[Category: Adhesin]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Outer membrane beta barrel]]		[[Category: Outer membrane beta barrel]]
	[[Category: Translocated intimin receptor]]		[[Category: Translocated intimin receptor]]

OCA at 07:58, 5 June 2014

2014-06-05T07:58:38Z

← Older revision		Revision as of 07:58, 5 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~4e1s~~.~~png~~\|~~left~~\|~~200px~~]]		==X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7==
			<StructureSection load='4e1s' size='340' side='right' caption='[[4e1s]], [[Resolution\|resolution]] 1.85Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1S FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e1t\|4e1t]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eae, eaeA, ECs4559, Intimin, L0025, Z5110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [http://www.ebi.ac.uk/pdbsum/4e1s PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Intimins and invasins are virulence factors produced by pathogenic Gram-negative bacteria. They contain C-terminal extracellular passenger domains that are involved in adhesion to host cells and N-terminal beta domains that are embedded in the outer membrane. Here, we identify the domain boundaries of an E. coli intimin beta domain and use this information to solve its structure and the beta domain structure of a Y. pseudotuberculosis invasin. Both beta domain structures crystallized as monomers and reveal that the previous range of residues assigned to the beta domain also includes a protease-resistant domain that is part of the passenger. Additionally, we identify 146 nonredundant representative members of the intimin/invasin family based on the boundaries of the highly conserved intimin and invasin beta domains. We then use this set of sequences along with our structural data to find and map the evolutionarily constrained residues within the beta domain.

	~~{{STRUCTURE_4e1s\| PDB=4e1s \| SCENE= }}~~		Crystal Structures of the Outer Membrane Domain of Intimin and Invasin from Enterohemorrhagic E. coli and Enteropathogenic Y. pseudotuberculosis.,Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK Structure. 2012 May 31. PMID:22658748<ref>PMID:22658748</ref>

	~~===X-ray crystal structure~~ of the ~~transmembrane beta-domain from intimin from EHEC strain O157:H7===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22658748}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[4e1s]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:022658748<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Barnard, T J.]]		[[Category: Barnard, T J.]]

OCA at 06:05, 25 July 2012

2012-07-25T06:05:22Z

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	[[Image:4e1s.~~jpg~~\|left\|200px]]		[[Image:4e1s.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_4e1s", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_4e1s\| PDB=4e1s \| SCENE= }}		{{STRUCTURE_4e1s\| PDB=4e1s \| SCENE= }}

	===X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7===		===X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22658748}}, adds the Publication Abstract to the page~~
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	{{ABSTRACT_PUBMED_22658748}}		{{ABSTRACT_PUBMED_22658748}}

OCA at 06:10, 13 June 2012

2012-06-13T06:10:57Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4e1s.jpg|left|200px]]
-The entry 4e1s is ON HOLD  until Paper Publication
+<!--
-Authors: Fairman, J.W., Dautin, N., Wojtowicz, D., Wei, L., Noinaj, N., Barnard, T.J., Udho, E., Finkelstein, A., Przytycka, T.M., Cherezov, V., Buchanan, S.K.
+===X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7===
-Description: X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7

OCA at 08:46, 16 May 2012

2012-05-16T08:46:05Z

← Older revision		Revision as of 08:46, 16 May 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4e1s is ON HOLD		The entry 4e1s is ON HOLD until Paper Publication

	Authors: Fairman, J.W., Dautin, N., Wojtowicz, D., Wei, L., Noinaj, N., Barnard, T.J., Udho, E., Finkelstein, A., Przytycka, T.M., Cherezov, V., Buchanan, S.K.		Authors: Fairman, J.W., Dautin, N., Wojtowicz, D., Wei, L., Noinaj, N., Barnard, T.J., Udho, E., Finkelstein, A., Przytycka, T.M., Cherezov, V., Buchanan, S.K.

	Description: X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7		Description: X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7

OCA: Protected "4e1s" [edit=sysop:move=sysop]

2012-03-14T06:16:20Z

Protected "4e1s" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:16, 14 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e1s is ON HOLD Authors: Fairman, J.W., Dautin, N., Wojtowicz, D., Wei, L., Noinaj, N., Barnard, T.J., Udho, E., Finkelstein, A., Przytycka, T.M., C...

2012-03-14T06:16:19Z

New page: '''Unreleased structure''' The entry 4e1s is ON HOLD Authors: Fairman, J.W., Dautin, N., Wojtowicz, D., Wei, L., Noinaj, N., Barnard, T.J., Udho, E., Finkelstein, A., Przytycka, T.M., C...

New page

'''Unreleased structure'''

The entry 4e1s is ON HOLD

Authors: Fairman, J.W., Dautin, N., Wojtowicz, D., Wei, L., Noinaj, N., Barnard, T.J., Udho, E., Finkelstein, A., Przytycka, T.M., Cherezov, V., Buchanan, S.K.

Description: X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7