4e1v - Revision history

OCA at 14:50, 14 March 2024

2024-03-14T14:50:07Z

← Older revision		Revision as of 14:50, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1v]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nsr 3nsr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1v]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nsr 3nsr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=URF:5-FLUOROURACIL'>URF</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=URF:5-FLUOROURACIL'>URF</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [https://pdbe.org/4e1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [https://www.ebi.ac.uk/pdbsum/4e1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [https://pdbe.org/4e1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [https://www.ebi.ac.uk/pdbsum/4e1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/UDP_SALTY UDP_SALTY]] Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).		[https://www.uniprot.org/uniprot/UDP_SALTY UDP_SALTY] Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Uridine phosphorylase (UPh), which is a key enzyme in the reutilization pathway of pyrimidine nucleoside metabolism, is a validated target for the treatment of infectious diseases and cancer. A detailed analysis of the interactions of UPh with the therapeutic ligand 5-fluorouracil (5-FUra) is important for the rational design of pharmacological inhibitors of these enzymes in prokaryotes and eukaryotes. Expanding on the preliminary analysis of the spatial organization of the active centre of UPh from the pathogenic bacterium Salmonella typhimurium (StUPh) in complex with 5-FUra [Lashkov et al. (2009), Acta Cryst. F65, 601-603], the X-ray structure of the StUPh-5-FUra complex was analysed at atomic resolution and an in silico model of the complex formed by the drug with UPh from Vibrio cholerae (VchUPh) was generated. These results should be considered in the design of selective inhibitors of UPhs from various species.

	X-ray structure of Salmonella typhimurium uridine phosphorylase complexed with 5-fluorouracil and molecular modelling of the complex of 5-fluorouracil with uridine phosphorylase from Vibrio cholerae.,Lashkov AA, Sotnichenko SE, Prokofiev II, Gabdulkhakov AG, Agapov II, Shtil AA, Betzel C, Mironov AS, Mikhailov AM Acta Crystallogr D Biol Crystallogr. 2012 Aug;68(Pt 8):968-74. Epub 2012 Jul 17. PMID:22868762<ref>PMID:22868762</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e1v" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Uridine phosphorylase\|Uridine phosphorylase]]		*[[Uridine phosphorylase\|Uridine phosphorylase]]
	*[[Uridine phosphorylase 3D structures\|Uridine phosphorylase 3D structures]]		*[[Uridine phosphorylase 3D structures\|Uridine phosphorylase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:45, 28 September 2022

2022-09-28T06:45:29Z

← Older revision		Revision as of 06:45, 28 September 2022
Line 1:		Line 1:

	==X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution==		==X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution==
	<StructureSection load='4e1v' size='340' side='right' caption='[[4e1v]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4e1v' size='340' side='right'caption='[[4e1v]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1v]] is a 9 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Salty Salty~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nsr 3nsr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E1V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1v]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nsr 3nsr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=URF:5-FLUOROURACIL'>URF</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=URF:5-FLUOROURACIL'>URF</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STM3968, STMD1.21, udp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [https://pdbe.org/4e1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [https://www.ebi.ac.uk/pdbsum/4e1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1v ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [~~http~~://pdbe.org/4e1v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e1v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/UDP_SALTY UDP_SALTY]] Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).		[[https://www.uniprot.org/uniprot/UDP_SALTY UDP_SALTY]] Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 21:
	==See Also==		==See Also==
	*[[Uridine phosphorylase\|Uridine phosphorylase]]		*[[Uridine phosphorylase\|Uridine phosphorylase]]
			*[[Uridine phosphorylase 3D structures\|Uridine phosphorylase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Salty~~]]		[[Category: Large Structures]]
	[[Category: ~~Uridine phosphorylase~~]]		[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
	[[Category: Gabdoulkhakov~~, A G~~]]		[[Category: Gabdoulkhakov AG]]
	[[Category: Lashkov~~, A A~~]]		[[Category: Lashkov AA]]
	[[Category: Mikhailov~~, A M~~]]		[[Category: Mikhailov AM]]
	[[Category: Prokofev~~, I I~~]]		[[Category: Prokofev II]]
	[[Category: Sotnichenko~~, S E]]~~		[[Category: Sotnichenko SE]]
	~~[[Category: Drug metabolism]]~~
	~~[[Category: Rossmann fold]]~~
	~~[[Category: Transferase~~]]

OCA at 10:04, 4 August 2016

2016-08-04T10:04:32Z

← Older revision		Revision as of 10:04, 4 August 2016
Line 1:		Line 1:

	==X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution==		==X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution==
	<StructureSection load='4e1v' size='340' side='right' caption='[[4e1v]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4e1v' size='340' side='right' caption='[[4e1v]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e1v]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Salmonella_enterica_subsp._enterica_serovar_typhimurium_str._lt2 Salmonella enterica subsp. enterica serovar typhimurium str. lt2~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nsr 3nsr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e1v]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nsr 3nsr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=URF:5-FLUOROURACIL'>URF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=URF:5-FLUOROURACIL'>URF</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STM3968, STMD1.21, udp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 ~~Salmonella enterica subsp. enterica serovar Typhimurium str. LT2~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STM3968, STMD1.21, udp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [http://www.ebi.ac.uk/pdbsum/4e1v PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [http://pdbe.org/4e1v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [http://www.ebi.ac.uk/pdbsum/4e1v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e1v" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Salmonella enterica subsp. enterica serovar typhimurium str. lt2~~]]		[[Category: Salty]]
	[[Category: Uridine phosphorylase]]		[[Category: Uridine phosphorylase]]
	[[Category: Gabdoulkhakov, A G]]		[[Category: Gabdoulkhakov, A G]]

OCA at 08:10, 25 December 2014

2014-12-25T08:10:05Z

← Older revision		Revision as of 08:10, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [http://www.ebi.ac.uk/pdbsum/4e1v PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [http://www.ebi.ac.uk/pdbsum/4e1v PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/UDP_SALTY UDP_SALTY]] Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:07, 9 December 2014

2014-12-09T15:07:36Z

← Older revision		Revision as of 15:07, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~4e1v~~.~~jpg~~\|~~left~~\|~~200px~~]]		==X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution==
			<StructureSection load='4e1v' size='340' side='right' caption='[[4e1v]], [[Resolution\|resolution]] 2.15Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4e1v]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium_str._lt2 Salmonella enterica subsp. enterica serovar typhimurium str. lt2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nsr 3nsr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1V FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=URF:5-FLUOROURACIL'>URF</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STM3968, STMD1.21, udp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1v RCSB], [http://www.ebi.ac.uk/pdbsum/4e1v PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Uridine phosphorylase (UPh), which is a key enzyme in the reutilization pathway of pyrimidine nucleoside metabolism, is a validated target for the treatment of infectious diseases and cancer. A detailed analysis of the interactions of UPh with the therapeutic ligand 5-fluorouracil (5-FUra) is important for the rational design of pharmacological inhibitors of these enzymes in prokaryotes and eukaryotes. Expanding on the preliminary analysis of the spatial organization of the active centre of UPh from the pathogenic bacterium Salmonella typhimurium (StUPh) in complex with 5-FUra [Lashkov et al. (2009), Acta Cryst. F65, 601-603], the X-ray structure of the StUPh-5-FUra complex was analysed at atomic resolution and an in silico model of the complex formed by the drug with UPh from Vibrio cholerae (VchUPh) was generated. These results should be considered in the design of selective inhibitors of UPhs from various species.

	~~{{STRUCTURE_4e1v\| PDB=4e1v \| SCENE= }}~~		X-ray structure of Salmonella typhimurium uridine phosphorylase complexed with 5-fluorouracil and molecular modelling of the complex of 5-fluorouracil with uridine phosphorylase from Vibrio cholerae.,Lashkov AA, Sotnichenko SE, Prokofiev II, Gabdulkhakov AG, Agapov II, Shtil AA, Betzel C, Mironov AS, Mikhailov AM Acta Crystallogr D Biol Crystallogr. 2012 Aug;68(Pt 8):968-74. Epub 2012 Jul 17. PMID:22868762<ref>PMID:22868762</ref>

	~~===X-RAY Structure~~ of the ~~Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2~~.~~15 A Resolution===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22868762}}~~		==See Also==
			*[[Uridine phosphorylase\|Uridine phosphorylase]]
	==~~About this Structure~~==		== References ==
	[[4e1v]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium_str._lt2 Salmonella enterica subsp. enterica serovar typhimurium str. lt2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nsr 3nsr]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1V OCA~~].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:019478441<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium str. lt2]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium str. lt2]]
	[[Category: Uridine phosphorylase]]		[[Category: Uridine phosphorylase]]
	[[Category: Gabdoulkhakov, A G.]]		[[Category: Gabdoulkhakov, A G]]
	[[Category: Lashkov, A A.]]		[[Category: Lashkov, A A]]
	[[Category: Mikhailov, A M.]]		[[Category: Mikhailov, A M]]
	[[Category: Prokofev, I I.]]		[[Category: Prokofev, I I]]
	[[Category: Sotnichenko, S E.]]		[[Category: Sotnichenko, S E]]
	[[Category: Drug metabolism]]		[[Category: Drug metabolism]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:09, 6 March 2013

2013-03-06T14:09:56Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4e1v.jpg|left|200px]]
-The entry 4e1v is ON HOLD  until Paper Publication
+{{STRUCTURE_4e1v|  PDB=4e1v  |  SCENE=  }}
-Authors: Lashkov, A.A., Sotnichenko, S.E., Prokofev, I.I., Gabdoulkhakov, A.G., Mikhailov, A.M.
+===X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution===
-Description: X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution
+{{ABSTRACT_PUBMED_22868762}}

OCA at 07:14, 21 March 2012

2012-03-21T07:14:45Z

← Older revision		Revision as of 07:14, 21 March 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4e1v is ON HOLD		The entry 4e1v is ON HOLD until Paper Publication

	Authors: Lashkov, A.A., Sotnichenko, S.E., Prokofev, I.I., Gabdoulkhakov, A.G., Mikhailov, A.M.		Authors: Lashkov, A.A., Sotnichenko, S.E., Prokofev, I.I., Gabdoulkhakov, A.G., Mikhailov, A.M.

	Description: X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution		Description: X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution

OCA: Protected "4e1v" [edit=sysop:move=sysop]

2012-03-14T06:16:25Z

Protected "4e1v" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:16, 14 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e1v is ON HOLD Authors: Lashkov, A.A., Sotnichenko, S.E., Prokofev, I.I., Gabdoulkhakov, A.G., Mikhailov, A.M. Description: X-RAY Structure of the...

2012-03-14T06:16:24Z

New page: '''Unreleased structure''' The entry 4e1v is ON HOLD Authors: Lashkov, A.A., Sotnichenko, S.E., Prokofev, I.I., Gabdoulkhakov, A.G., Mikhailov, A.M. Description: X-RAY Structure of the...

New page

'''Unreleased structure'''

The entry 4e1v is ON HOLD

Authors: Lashkov, A.A., Sotnichenko, S.E., Prokofev, I.I., Gabdoulkhakov, A.G., Mikhailov, A.M.

Description: X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with 5-Fluorouracil at 2.15 A Resolution