4e2i - Revision history

OCA at 11:01, 1 March 2024

2024-03-01T11:01:33Z

← Older revision		Revision as of 11:01, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 5Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [https://pdbe.org/4e2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [https://www.ebi.ac.uk/pdbsum/4e2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [https://pdbe.org/4e2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [https://www.ebi.ac.uk/pdbsum/4e2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/LT_SV40 LT_SV40]] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7.<ref>PMID:8647434</ref> <ref>PMID:9632777</ref> <ref>PMID:9488456</ref> <ref>PMID:15680424</ref> <ref>PMID:15611062</ref> <ref>PMID:17341466</ref> <ref>PMID:18922873</ref> Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain.<ref>PMID:8647434</ref> <ref>PMID:9632777</ref> <ref>PMID:9488456</ref> <ref>PMID:15680424</ref> <ref>PMID:15611062</ref> <ref>PMID:17341466</ref> <ref>PMID:18922873</ref>		[https://www.uniprot.org/uniprot/LT_SV40 LT_SV40] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7.<ref>PMID:8647434</ref> <ref>PMID:9632777</ref> <ref>PMID:9488456</ref> <ref>PMID:15680424</ref> <ref>PMID:15611062</ref> <ref>PMID:17341466</ref> <ref>PMID:18922873</ref> Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain.<ref>PMID:8647434</ref> <ref>PMID:9632777</ref> <ref>PMID:9488456</ref> <ref>PMID:15680424</ref> <ref>PMID:15611062</ref> <ref>PMID:17341466</ref> <ref>PMID:18922873</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	DNA polymerase alpha-primase (Pol-prim) plays an essential role in eukaryotic DNA replication, initiating synthesis of the leading strand and of each Okazaki fragment on the lagging strand. Pol-prim is composed of a primase heterodimer that synthesizes an RNA primer, a DNA polymerase subunit that extends the primer, and a regulatory B-subunit (p68) without apparent enzymatic activity. Pol-prim is thought to interact with eukaryotic replicative helicases, forming a dynamic multiprotein assembly that displays primosome activity. At least three subunits of Pol-prim interact physically with the hexameric replicative helicase SV40 large T antigen, constituting a simple primosome that is active in vitro. However, structural understanding of these interactions and their role in viral chromatin replication in vivo remains incomplete. Here, we report the detailed large T antigen-p68 interface, as revealed in a co-crystal structure and validated by site-directed mutagenesis, and we demonstrate its functional importance in activating the SV40 primosome in cell-free reactions with purified Pol-prim, as well as in monkey cells in vivo.

	Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase alpha-primase.,Zhou B, Arnett DR, Yu X, Brewster A, Sowd GA, Xie CL, Vila S, Gai D, Fanning E, Chen XS J Biol Chem. 2012 Aug 3;287(32):26854-66. doi: 10.1074/jbc.M112.363655. Epub 2012, Jun 14. PMID:22700977<ref>PMID:22700977</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e2i" style="background-color:#fffaf0;"></div~~>

	==See Also==		==See Also==
			*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	*[[Large T Antigen\|Large T Antigen]]		*[[Large T Antigen\|Large T Antigen]]
	== References ==		== References ==

OCA at 06:46, 28 September 2022

2022-09-28T06:46:20Z

← Older revision		Revision as of 06:46, 28 September 2022
Line 1:		Line 1:

	==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase==		==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase==
	<StructureSection load='4e2i' size='340' side='right' caption='[[4e2i]], [[Resolution\|resolution]] 5.00Å' scene=''>		<StructureSection load='4e2i' size='340' side='right'caption='[[4e2i]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Polyomavirus_maccacae Polyomavirus maccacae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E2I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [https://pdbe.org/4e2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [https://www.ebi.ac.uk/pdbsum/4e2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2i ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [~~http~~://pdbe.org/4e2i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e2i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/~~DPOA2_HUMAN DPOA2_HUMAN~~]] ~~May play an~~ essential role at the early ~~stage~~ of ~~chromosomal~~ DNA replication by ~~coupling~~ the ~~polymerase alpha/primase~~ complex to the ~~cellular replication machinery (By similarity)~~.		[[https://www.uniprot.org/uniprot/LT_SV40 LT_SV40]] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7.<ref>PMID:8647434</ref> <ref>PMID:9632777</ref> <ref>PMID:9488456</ref> <ref>PMID:15680424</ref> <ref>PMID:15611062</ref> <ref>PMID:17341466</ref> <ref>PMID:18922873</ref> Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain.<ref>PMID:8647434</ref> <ref>PMID:9632777</ref> <ref>PMID:9488456</ref> <ref>PMID:15680424</ref> <ref>PMID:15611062</ref> <ref>PMID:17341466</ref> <ref>PMID:18922873</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
	*[[DNA polymerase\|DNA polymerase]]
	*[[Large T Antigen\|Large T Antigen]]		*[[Large T Antigen\|Large T Antigen]]
	== References ==		== References ==
Line 27:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: ~~Polyomavirus maccacae~~]]		[[Category: Large Structures]]
	[[Category: Arnett~~, D R~~]]		[[Category: Macaca mulatta polyomavirus 1]]
	[[Category: Brewster, A]]		[[Category: Arnett DR]]
	[[Category: Chen~~, X S~~]]		[[Category: Brewster A]]
	[[Category: Fanning, E]]		[[Category: Chen XS]]
	[[Category: Gai, D]]		[[Category: Fanning E]]
	[[Category: Sowd~~, G A~~]]		[[Category: Gai D]]
	[[Category: Vila, S]]		[[Category: Sowd GA]]
	[[Category: Xie~~, C L~~]]		[[Category: Vila S]]
	[[Category: Yu, X]]		[[Category: Xie CL]]
	[[Category: Zhou, B]]		[[Category: Yu X]]
	~~[[Category: Hydrolase-dna binding complex]]~~		[[Category: Zhou B]]
	~~[[Category: Hydrolase-dna binding protein complex]]~~
	~~[[Category: Replication initiation~~]]

OCA at 18:00, 4 August 2016

2016-08-04T18:00:48Z

← Older revision		Revision as of 18:00, 4 August 2016
Line 1:		Line 1:

	==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase==		==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase==
	<StructureSection load='4e2i' size='340' side='right' caption='[[4e2i]], [[Resolution\|resolution]] 5.00Å' scene=''>		<StructureSection load='4e2i' size='340' side='right' caption='[[4e2i]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~] and [http://en.wikipedia.org/wiki/~~Simian_virus_40 Simian virus 40~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Polyomavirus_maccacae Polyomavirus maccacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [http://www.ebi.ac.uk/pdbsum/4e2i PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [http://pdbe.org/4e2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [http://www.ebi.ac.uk/pdbsum/4e2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e2i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: ~~Simian virus 40~~]]		[[Category: Polyomavirus maccacae]]
	[[Category: Arnett, D R]]		[[Category: Arnett, D R]]
	[[Category: Brewster, A]]		[[Category: Brewster, A]]

OCA at 11:41, 25 December 2014

2014-12-25T11:41:24Z

← Older revision		Revision as of 11:41, 25 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4e2i\| PDB=4e2i \| SCENE= }}~~		==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase==
	===The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase===		<StructureSection load='4e2i' size='340' side='right' caption='[[4e2i]], [[Resolution\|resolution]] 5.00Å' scene=''>
	~~{{ABSTRACT_PUBMED_22700977}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2I FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [http://www.ebi.ac.uk/pdbsum/4e2i PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			DNA polymerase alpha-primase (Pol-prim) plays an essential role in eukaryotic DNA replication, initiating synthesis of the leading strand and of each Okazaki fragment on the lagging strand. Pol-prim is composed of a primase heterodimer that synthesizes an RNA primer, a DNA polymerase subunit that extends the primer, and a regulatory B-subunit (p68) without apparent enzymatic activity. Pol-prim is thought to interact with eukaryotic replicative helicases, forming a dynamic multiprotein assembly that displays primosome activity. At least three subunits of Pol-prim interact physically with the hexameric replicative helicase SV40 large T antigen, constituting a simple primosome that is active in vitro. However, structural understanding of these interactions and their role in viral chromatin replication in vivo remains incomplete. Here, we report the detailed large T antigen-p68 interface, as revealed in a co-crystal structure and validated by site-directed mutagenesis, and we demonstrate its functional importance in activating the SV40 primosome in cell-free reactions with purified Pol-prim, as well as in monkey cells in vivo.

	~~==Function==~~		Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase alpha-primase.,Zhou B, Arnett DR, Yu X, Brewster A, Sowd GA, Xie CL, Vila S, Gai D, Fanning E, Chen XS J Biol Chem. 2012 Aug 3;287(32):26854-66. doi: 10.1074/jbc.M112.363655. Epub 2012, Jun 14. PMID:22700977<ref>PMID:22700977</ref>
	~~[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at~~ the ~~early stage~~ of ~~chromosomal~~ DNA ~~replication by coupling the~~ polymerase alpha/primase ~~complex to the cellular replication machinery~~ (~~By similarity~~).

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[4e2i]] is~~ a ~~23 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens] and [http://en~~.~~wikipedia~~.~~org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=4E2I OCA].~~		</div>

	==See Also==		==See Also==
	*[[DNA polymerase\|DNA polymerase]]		*[[DNA polymerase\|DNA polymerase]]
			*[[Large T Antigen\|Large T Antigen]]
	==~~Reference~~==		== References ==
	~~<ref group="xtra">PMID:022700977</ref>~~<references ~~group="xtra"~~/><~~references~~/>		<references/>
			__TOC__
			</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Simian virus 40]]		[[Category: Simian virus 40]]
	[[Category: Arnett, D R.]]		[[Category: Arnett, D R]]
	[[Category: Brewster, A.]]		[[Category: Brewster, A]]
	[[Category: Chen, X S.]]		[[Category: Chen, X S]]
	[[Category: Fanning, E.]]		[[Category: Fanning, E]]
	[[Category: Gai, D.]]		[[Category: Gai, D]]
	[[Category: Sowd, G A.]]		[[Category: Sowd, G A]]
	[[Category: Vila, S.]]		[[Category: Vila, S]]
	[[Category: Xie, C L.]]		[[Category: Xie, C L]]
	[[Category: Yu, X.]]		[[Category: Yu, X]]
	[[Category: Zhou, B.]]		[[Category: Zhou, B]]
	[[Category: Hydrolase-dna binding complex]]		[[Category: Hydrolase-dna binding complex]]
	[[Category: Hydrolase-dna binding protein complex]]		[[Category: Hydrolase-dna binding protein complex]]
	[[Category: Replication initiation]]		[[Category: Replication initiation]]

OCA at 04:58, 28 August 2013

2013-08-28T04:58:53Z

@@ Line 1: / Line 1: @@
 {{STRUCTURE_4e2i|  PDB=4e2i  |  SCENE=  }}
 ===The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase===
 ==About this Structure==
 [[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA].
 [[Category: Homo sapiens]]
 [[Category: Simian virus 40]]

OCA at 06:23, 13 June 2012

2012-06-13T06:23:33Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4e2i.jpg|left|200px]]
-The entry 4e2i is ON HOLD
+<!--
-Authors: Zhou, B., Arnett, D.R., Yu, X., Brewster, A., Sowd, G.A., Xie, C.L., Vila, S., Gai, D., Fanning, E., Chen, X.S.
+===The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase===
-Description: The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase

OCA: Protected "4e2i" [edit=sysop:move=sysop]

2012-03-14T06:17:09Z

Protected "4e2i" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:17, 14 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e2i is ON HOLD Authors: Zhou, B., Arnett, D.R., Yu, X., Brewster, A., Sowd, G.A., Xie, C.L., Vila, S., Gai, D., Fanning, E., Chen, X.S. Descriptio...

2012-03-14T06:17:08Z

New page: '''Unreleased structure''' The entry 4e2i is ON HOLD Authors: Zhou, B., Arnett, D.R., Yu, X., Brewster, A., Sowd, G.A., Xie, C.L., Vila, S., Gai, D., Fanning, E., Chen, X.S. Descriptio...

New page

'''Unreleased structure'''

The entry 4e2i is ON HOLD

Authors: Zhou, B., Arnett, D.R., Yu, X., Brewster, A., Sowd, G.A., Xie, C.L., Vila, S., Gai, D., Fanning, E., Chen, X.S.

Description: The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase