4e2l - Revision history

OCA at 11:01, 1 March 2024

2024-03-01T11:01:34Z

← Older revision		Revision as of 11:01, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2l]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2L FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2l]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2L FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2l OCA], [https://pdbe.org/4e2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2l RCSB], [https://www.ebi.ac.uk/pdbsum/4e2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2l ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2l OCA], [https://pdbe.org/4e2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2l RCSB], [https://www.ebi.ac.uk/pdbsum/4e2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Q8XBV8_ECO57 Q8XBV8_ECO57]]		[https://www.uniprot.org/uniprot/Q8XBV8_ECO57 Q8XBV8_ECO57]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The surface O-antigen polymers of gram-negative bacteria exhibit a modal length distribution that depends on dedicated chain-length regulator periplasmic proteins (Polysaccharide Co-Polymerases, PCPs) anchored in the inner membrane by two transmembrane helices. In an attempt to determine whether structural changes underlie the O-antigen modal length specification we have determined crystal structures of several closely related PCPs, namely two chimeric PCP-1 family members solved at 1.5A and 2.6A and a wild-type PCP-1 from Shigella flexneri solved at 2.8A. The chimeric proteins form circular octamers while the wild type WzzB from Shigella flexneri was found to be an open trimer. We also present the structure of a WzzBFepE mutant, which exhibits severe attenuation in its ability to produce very long O-antigen polymers. Our findings suggest that the differences in the modal length distribution depend primarily on the surface-exposed amino acids in specific regions rather than on the differences in the oligomeric state of the PCP protomers.

	~~Structural characterization of closely related o-antigen LPS-chain length regulators.,Kalynych S, Yao D, Magee J, Cygler M J Biol Chem. 2012 Mar 21. PMID:22437828<ref>PMID:22437828</ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e2l" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:46, 28 September 2022

2022-09-28T06:46:26Z

← Older revision		Revision as of 06:46, 28 September 2022
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	==Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein==		==Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein==
	<StructureSection load='4e2l' size='340' side='right' caption='[[4e2l]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4e2l' size='340' side='right'caption='[[4e2l]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2l]] is a 9 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E2L FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2l]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2L FirstGlance]. <br>
	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e29\|4e29]], [[4e2c\|4e2c]], [[4e2h\|4e2h]]</td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2l OCA], [https://pdbe.org/4e2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2l RCSB], [https://www.ebi.ac.uk/pdbsum/4e2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2l ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECs0626, fepE, Z0728 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2l OCA], [~~http~~://pdbe.org/4e2l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e2l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e2l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2l ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/Q8XBV8_ECO57 Q8XBV8_ECO57]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Eco57]]~~		[[Category: Escherichia coli O157:H7]]
	~~[[Category~~: ~~Cygler, M~~]]		[[Category: Large Structures]]
	[[Category: ~~Kalynych, S~~]]		[[Category: Cygler M]]
	[[Category: ~~Magee, J D~~]]		[[Category: Kalynych S]]
	[[Category: ~~Yao, D~~]]		[[Category: Magee JD]]
	[[Category: ~~Fepe polysaccharride co-polymerase~~]]		[[Category: Yao D]]
	[[Category: ~~Inner membrane]]~~
	~~[[Category: Membrane protein]]~~
	~~[[Category: Periplasmic space]]~~
	~~[[Category: Wzz~~]]

OCA at 20:27, 11 August 2016

2016-08-11T20:27:32Z

← Older revision		Revision as of 20:27, 11 August 2016
Line 1:		Line 1:

	==Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein==		==Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein==
	<StructureSection load='4e2l' size='340' side='right' caption='[[4e2l]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4e2l' size='340' side='right' caption='[[4e2l]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2l]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2L FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2l]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2L FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e29\|4e29]], [[4e2c\|4e2c]], [[4e2h\|4e2h]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e29\|4e29]], [[4e2c\|4e2c]], [[4e2h\|4e2h]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECs0626, fepE, Z0728 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECs0626, fepE, Z0728 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2l RCSB], [http://www.ebi.ac.uk/pdbsum/4e2l PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2l OCA], [http://pdbe.org/4e2l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e2l RCSB], [http://www.ebi.ac.uk/pdbsum/4e2l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2l ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e2l" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Escherichia coli~~]]		[[Category: Eco57]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]
	[[Category: Kalynych, S]]		[[Category: Kalynych, S]]

OCA at 13:30, 4 January 2015

2015-01-04T13:30:16Z

← Older revision		Revision as of 13:30, 4 January 2015
Line 1:		Line 1:
	[[~~Image~~:~~4e2l~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein==
			<StructureSection load='4e2l' size='340' side='right' caption='[[4e2l]], [[Resolution\|resolution]] 2.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4e2l]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2L FirstGlance]. <br>
			</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e29\|4e29]], [[4e2c\|4e2c]], [[4e2h\|4e2h]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECs0626, fepE, Z0728 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2l RCSB], [http://www.ebi.ac.uk/pdbsum/4e2l PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The surface O-antigen polymers of gram-negative bacteria exhibit a modal length distribution that depends on dedicated chain-length regulator periplasmic proteins (Polysaccharide Co-Polymerases, PCPs) anchored in the inner membrane by two transmembrane helices. In an attempt to determine whether structural changes underlie the O-antigen modal length specification we have determined crystal structures of several closely related PCPs, namely two chimeric PCP-1 family members solved at 1.5A and 2.6A and a wild-type PCP-1 from Shigella flexneri solved at 2.8A. The chimeric proteins form circular octamers while the wild type WzzB from Shigella flexneri was found to be an open trimer. We also present the structure of a WzzBFepE mutant, which exhibits severe attenuation in its ability to produce very long O-antigen polymers. Our findings suggest that the differences in the modal length distribution depend primarily on the surface-exposed amino acids in specific regions rather than on the differences in the oligomeric state of the PCP protomers.

	~~{{STRUCTURE_4e2l\| PDB=4e2l \| SCENE= }}~~		Structural characterization of closely related o-antigen LPS-chain length regulators.,Kalynych S, Yao D, Magee J, Cygler M J Biol Chem. 2012 Mar 21. PMID:22437828<ref>PMID:22437828</ref>

	~~===Crystal Structure~~ of the ~~periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22437828}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[4e2l]] is a 9 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:022437828<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Cygler, M.]]		[[Category: Cygler, M]]
	[[Category: Kalynych, S.]]		[[Category: Kalynych, S]]
	[[Category: Magee, J D.]]		[[Category: Magee, J D]]
	[[Category: Yao, D.]]		[[Category: Yao, D]]
	[[Category: Fepe polysaccharride co-polymerase]]		[[Category: Fepe polysaccharride co-polymerase]]
	[[Category: Inner membrane]]		[[Category: Inner membrane]]

OCA at 08:05, 20 June 2012

2012-06-20T08:05:53Z

← Older revision		Revision as of 08:05, 20 June 2012
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	[[Image:4e2l.png\|left\|200px]]		[[Image:4e2l.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_4e2l", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_4e2l\| PDB=4e2l \| SCENE= }}		{{STRUCTURE_4e2l\| PDB=4e2l \| SCENE= }}

	===Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein===		===Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22437828}}, adds the Publication Abstract to the page~~
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	~~-->~~
	{{ABSTRACT_PUBMED_22437828}}		{{ABSTRACT_PUBMED_22437828}}

OCA at 05:27, 4 April 2012

2012-04-04T05:27:32Z

← Older revision		Revision as of 05:27, 4 April 2012
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	[[Image:4e2l.~~jpg~~\|left\|200px]]		[[Image:4e2l.png\|left\|200px]]

	<!--		<!--
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	===Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein===		===Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22437828}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22437828 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22437828}}

	==About this Structure==		==About this Structure==
	[[4e2l]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA].		[[4e2l]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2L OCA].

			==Reference==
			<ref group="xtra">PMID:022437828</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Cygler, M.]]		[[Category: Cygler, M.]]

OCA at 07:54, 28 March 2012

2012-03-28T07:54:56Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4e2l.jpg|left|200px]]
-The entry 4e2l is ON HOLD
+<!--
-Authors: Kalynych, S., Yao, D., Magee, J.D., Cygler, M.
+===Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein===
-Description: Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein

OCA: Protected "4e2l" [edit=sysop:move=sysop]

2012-03-14T06:17:16Z

Protected "4e2l" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:17, 14 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e2l is ON HOLD Authors: Kalynych, S., Yao, D., Magee, J.D., Cygler, M. Description: Crystal Structure of the periplasmic domain of mutant FepE LPS...

2012-03-14T06:17:14Z

New page: '''Unreleased structure''' The entry 4e2l is ON HOLD Authors: Kalynych, S., Yao, D., Magee, J.D., Cygler, M. Description: Crystal Structure of the periplasmic domain of mutant FepE LPS...

New page

'''Unreleased structure'''

The entry 4e2l is ON HOLD

Authors: Kalynych, S., Yao, D., Magee, J.D., Cygler, M.

Description: Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein