4e2v - Revision history

OCA at 14:50, 14 March 2024

2024-03-14T14:50:33Z

← Older revision		Revision as of 14:50, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zymomonas_mobilis Zymomonas mobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zymomonas_mobilis Zymomonas mobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.18Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2v OCA], [https://pdbe.org/4e2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2v RCSB], [https://www.ebi.ac.uk/pdbsum/4e2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2v OCA], [https://pdbe.org/4e2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2v RCSB], [https://www.ebi.ac.uk/pdbsum/4e2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]		[https://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Bacterial tRNA-guanine transglycosylase (Tgt) catalyses the exchange of the genetically encoded guanine at the wobble position of tRNAs(His,Tyr,Asp,Asn) by the premodified base preQ1, which is further converted to queuine at the tRNA level. As eucaryotes are not able to synthesise queuine de novo but acquire it through their diet, eucaryotic Tgt directly inserts the hypermodified base into the wobble position of the tRNAs mentioned above. Bacterial Tgt is required for the efficient pathogenicity of Shigella sp, the causative agent of bacillary dysentery and, hence, it constitutes a putative target for the rational design of anti-Shigellosis compounds. Since mammalian Tgt is known to be indirectly essential to the conversion of phenylalanine to tyrosine, it is necessary to create substances which only inhibit bacterial but not eucaryotic Tgt. Therefore, it seems of utmost importance to study selectivity-determining features within both types of proteins. Homology models of Caenorhabditis elegans Tgt and human Tgt suggest that the replacement of Cys158 and Val233 in bacterial Tgt (Zymomonas mobilis Tgt numbering) by valine and accordingly glycine in eucaryotic Tgt largely accounts for the different substrate specificities. In the present study we have created mutated variants of Z. mobilis Tgt in order to investigate the impact of a Cys158Val and a Val233Gly exchange on catalytic activity and substrate specificity. Using enzyme kinetics and X-ray crystallography, we gained evidence that the Cys158Val mutation reduces the affinity to preQ1 while leaving the affinity to guanine unaffected. The Val233Gly exchange leads to an enlarged substrate binding pocket, that is necessary to accommodate queuine in a conformation compatible with the intermediately covalently bound tRNA molecule. Contrary to our expectations, we found that a priori queuine is recognised by the binding pocket of bacterial Tgt without, however, being used as a substrate.

	Investigation of specificity determinants in bacterial tRNA-guanine transglycosylase reveals queuine, the substrate of its eucaryotic counterpart, as inhibitor.,Biela I, Tidten-Luksch N, Immekus F, Glinca S, Nguyen TX, Gerber HD, Heine A, Klebe G, Reuter K PLoS One. 2013 May 21;8(5):e64240. doi: 10.1371/journal.pone.0064240. Print 2013. PMID:23704982<ref>PMID:23704982</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e2v" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[TRNA-guanine transglycosylase 3D structures\|TRNA-guanine transglycosylase 3D structures]]		*[[TRNA-guanine transglycosylase 3D structures\|TRNA-guanine transglycosylase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:46, 28 September 2022

2022-09-28T06:46:52Z

← Older revision		Revision as of 06:46, 28 September 2022
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	==tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1==		==tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1==
	<StructureSection load='4e2v' size='340' side='right' caption='[[4e2v]], [[Resolution\|resolution]] 1.18Å' scene=''>		<StructureSection load='4e2v' size='340' side='right'caption='[[4e2v]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2v]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"achromobacter_anaerobium"_(sic)_shimwell_1937 "achromobacter anaerobium" (sic) shimwell 1937~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E2V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zymomonas_mobilis Zymomonas mobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bl3\|3bl3]], [[3bld\|3bld]], [[3bll\|3bll]], [[3blo\|3blo]], [[2nso\|2nso]], [[2nqz\|2nqz]]</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2v OCA], [https://pdbe.org/4e2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2v RCSB], [https://www.ebi.ac.uk/pdbsum/4e2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2v ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tgt, ZMO0363 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=542 "Achromobacter anaerobium" (sic) Shimwell 1937])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-guanine(34)_transglycosylase tRNA-guanine(34) transglycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2v OCA], [~~http~~://pdbe.org/4e2v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e2v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e2v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]		[[https://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4e2v" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4e2v" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[TRNA-guanine transglycosylase 3D structures\|TRNA-guanine transglycosylase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Heine, A~~]]		[[Category: Large Structures]]
	[[Category: ~~Klebe, G~~]]		[[Category: Zymomonas mobilis]]
	[[Category: ~~Reuter, K~~]]		[[Category: Heine A]]
	[[Category: ~~Schmidt, I~~]]		[[Category: Klebe G]]
	[[Category: ~~Tidten, N~~]]		[[Category: Reuter K]]
	[[Category: ~~Bacterial tgt~~]]		[[Category: Schmidt I]]
	[[Category: ~~Guanin]]~~		[[Category: Tidten N]]
	~~[[Category: Guanine exchange enzyme]]~~
	~~[[Category: Preq1]]~~
	~~[[Category: Substrate specificity]]~~
	~~[[Category: Transferase-transferase inhibitor complex]]~~
	~~[[Category: Trna~~]]

OCA at 07:59, 15 November 2017

2017-11-15T07:59:00Z

← Older revision		Revision as of 07:59, 15 November 2017
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 4e2v" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4e2v" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[TRNA-guanine transglycosylase\|TRNA-guanine transglycosylase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:23, 5 August 2016

2016-08-05T10:23:07Z

← Older revision		Revision as of 10:23, 5 August 2016
Line 1:		Line 1:

	==tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1==		==tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1==
	<StructureSection load='4e2v' size='340' side='right' caption='[[4e2v]], [[Resolution\|resolution]] 1.18Å' scene=''>		<StructureSection load='4e2v' size='340' side='right' caption='[[4e2v]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e2v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Zymomonas_mobilis Zymomonas mobilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e2v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_anaerobium"_(sic)_shimwell_1937 "achromobacter anaerobium" (sic) shimwell 1937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bl3\|3bl3]], [[3bld\|3bld]], [[3bll\|3bll]], [[3blo\|3blo]], [[2nso\|2nso]], [[2nqz\|2nqz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bl3\|3bl3]], [[3bld\|3bld]], [[3bll\|3bll]], [[3blo\|3blo]], [[2nso\|2nso]], [[2nqz\|2nqz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tgt, ZMO0363 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=542 ~~Zymomonas mobilis~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tgt, ZMO0363 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=542 "Achromobacter anaerobium" (sic) Shimwell 1937])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-guanine(34)_transglycosylase tRNA-guanine(34) transglycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-guanine(34)_transglycosylase tRNA-guanine(34) transglycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2v RCSB], [http://www.ebi.ac.uk/pdbsum/4e2v PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2v OCA], [http://pdbe.org/4e2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e2v RCSB], [http://www.ebi.ac.uk/pdbsum/4e2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e2v" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Zymomonas mobilis]]~~
	[[Category: Heine, A]]		[[Category: Heine, A]]
	[[Category: Klebe, G]]		[[Category: Klebe, G]]

OCA at 01:34, 25 December 2014

2014-12-25T01:34:36Z

← Older revision		Revision as of 01:34, 25 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4e2v~~\| ~~PDB~~=4e2v \| ~~SCENE~~= }}		==tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1==
	===tRNA-guanine transglycosylase ~~Y106F~~, ~~C158V mutant~~ in ~~complex~~ with ~~preQ1~~===		<StructureSection load='4e2v' size='340' side='right' caption='[[4e2v]], [[Resolution\|resolution]] 1.18Å' scene=''>
	~~{{ABSTRACT_PUBMED_23704982}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4e2v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zymomonas_mobilis Zymomonas mobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2V FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bl3\|3bl3]], [[3bld\|3bld]], [[3bll\|3bll]], [[3blo\|3blo]], [[2nso\|2nso]], [[2nqz\|2nqz]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tgt, ZMO0363 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=542 Zymomonas mobilis])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-guanine(34)_transglycosylase tRNA-guanine(34) transglycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2v RCSB], [http://www.ebi.ac.uk/pdbsum/4e2v PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Bacterial tRNA-guanine transglycosylase (Tgt) catalyses the exchange of the genetically encoded guanine at the wobble position of tRNAs(His,Tyr,Asp,Asn) by the premodified base preQ1, which is further converted to queuine at the tRNA level. As eucaryotes are not able to synthesise queuine de novo but acquire it through their diet, eucaryotic Tgt directly inserts the hypermodified base into the wobble position of the tRNAs mentioned above. Bacterial Tgt is required for the efficient pathogenicity of Shigella sp, the causative agent of bacillary dysentery and, hence, it constitutes a putative target for the rational design of anti-Shigellosis compounds. Since mammalian Tgt is known to be indirectly essential to the conversion of phenylalanine to tyrosine, it is necessary to create substances which only inhibit bacterial but not eucaryotic Tgt. Therefore, it seems of utmost importance to study selectivity-determining features within both types of proteins. Homology models of Caenorhabditis elegans Tgt and human Tgt suggest that the replacement of Cys158 and Val233 in bacterial Tgt (Zymomonas mobilis Tgt numbering) by valine and accordingly glycine in eucaryotic Tgt largely accounts for the different substrate specificities. In the present study we have created mutated variants of Z. mobilis Tgt in order to investigate the impact of a Cys158Val and a Val233Gly exchange on catalytic activity and substrate specificity. Using enzyme kinetics and X-ray crystallography, we gained evidence that the Cys158Val mutation reduces the affinity to preQ1 while leaving the affinity to guanine unaffected. The Val233Gly exchange leads to an enlarged substrate binding pocket, that is necessary to accommodate queuine in a conformation compatible with the intermediately covalently bound tRNA molecule. Contrary to our expectations, we found that a priori queuine is recognised by the binding pocket of bacterial Tgt without, however, being used as a substrate.

	~~==Function==~~		Investigation of specificity determinants in bacterial tRNA-guanine transglycosylase reveals queuine, the substrate of its eucaryotic counterpart, as inhibitor.,Biela I, Tidten-Luksch N, Immekus F, Glinca S, Nguyen TX, Gerber HD, Heine A, Klebe G, Reuter K PLoS One. 2013 May 21;8(5):e64240. doi: 10.1371/journal.pone.0064240. Print 2013. PMID:23704982<ref>PMID:23704982</ref>
	~~[[http://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine~~ in ~~tRNAs with GU(N) anticodons (~~tRNA-~~Asp~~, ~~-Asn~~, ~~-His and -Tyr)~~. ~~After this exchange~~, ~~a cyclopentendiol moiety is attached to the 7~~-~~aminomethyl group of 7-deazaguanine~~, ~~resulting in the hypermodified nucleoside queuosine~~ (~~Q) (7-(((4,~~5~~-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine~~).~~[HAMAP-Rule~~:~~MF_00168]~~

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[4e2v]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Zymomonas_mobilis Zymomonas mobilis]~~. ~~Full crystallographic information is available from [http:/~~/~~oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA].~~		</div>

	==See Also==		==See Also==
	*[[TRNA-guanine transglycosylase\|TRNA-guanine transglycosylase]]		*[[TRNA-guanine transglycosylase\|TRNA-guanine transglycosylase]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:023704982</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Zymomonas mobilis]]		[[Category: Zymomonas mobilis]]
	[[Category: Heine, A.]]		[[Category: Heine, A]]
	[[Category: Klebe, G.]]		[[Category: Klebe, G]]
	[[Category: Reuter, K.]]		[[Category: Reuter, K]]
	[[Category: Schmidt, I.]]		[[Category: Schmidt, I]]
	[[Category: Tidten, N.]]		[[Category: Tidten, N]]
	[[Category: Bacterial tgt]]		[[Category: Bacterial tgt]]
	[[Category: Guanin]]		[[Category: Guanin]]

OCA at 04:36, 18 July 2013

2013-07-18T04:36:08Z

← Older revision		Revision as of 04:36, 18 July 2013
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	{{STRUCTURE_4e2v\| PDB=4e2v \| SCENE= }}		{{STRUCTURE_4e2v\| PDB=4e2v \| SCENE= }}
	===tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1===		===tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1===
			{{ABSTRACT_PUBMED_23704982}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4e2v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zymomonas_mobilis Zymomonas mobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA].		[[4e2v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zymomonas_mobilis Zymomonas mobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA].

			==See Also==
			*[[TRNA-guanine transglycosylase\|TRNA-guanine transglycosylase]]

			==Reference==
			<ref group="xtra">PMID:023704982</ref><references group="xtra"/><references/>
	[[Category: Zymomonas mobilis]]		[[Category: Zymomonas mobilis]]
	[[Category: Heine, A.]]		[[Category: Heine, A.]]

OCA at 08:57, 27 March 2013

2013-03-27T08:57:23Z

← Older revision		Revision as of 08:57, 27 March 2013
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	~~'''Unreleased structure'''~~		{{STRUCTURE_4e2v\| PDB=4e2v \| SCENE= }}
			===tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1===

	~~The entry 4e2v~~ is ~~ON HOLD until Paper Publication~~		==Function==
			[[http://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]

	~~Authors~~: ~~Schmidt, I~~., ~~Reuter, K~~., Klebe, G., ~~Heine~~, A., Tidten, N.		==About this Structure==
			[[4e2v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zymomonas_mobilis Zymomonas mobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2V OCA].
	~~Description~~: ~~tRNA~~-~~guanine transglycosylase Y106F, C158V mutant in~~ complex ~~with preQ1~~		[[Category: Zymomonas mobilis]]
			[[Category: Heine, A.]]
			[[Category: Klebe, G.]]
			[[Category: Reuter, K.]]
			[[Category: Schmidt, I.]]
			[[Category: Tidten, N.]]
			[[Category: Bacterial tgt]]
			[[Category: Guanin]]
			[[Category: Guanine exchange enzyme]]
			[[Category: Preq1]]
			[[Category: Substrate specificity]]
			[[Category: Transferase-transferase inhibitor complex]]
			[[Category: Trna]]

OCA at 07:21, 28 March 2012

2012-03-28T07:21:42Z

← Older revision		Revision as of 07:21, 28 March 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4e2v is ON HOLD		The entry 4e2v is ON HOLD until Paper Publication

	Authors: Schmidt, I., Reuter, K., Klebe, G., Heine, A., Tidten, N.		Authors: Schmidt, I., Reuter, K., Klebe, G., Heine, A., Tidten, N.

	Description: tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1		Description: tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1

OCA: Protected "4e2v" [edit=sysop:move=sysop]

2012-03-21T07:15:13Z

Protected "4e2v" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:15, 21 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e2v is ON HOLD Authors: Schmidt, I., Reuter, K., Klebe, G., Heine, A., Tidten, N. Description: tRNA-guanine transglycosylase Y106F, C158V mutant i...

2012-03-21T07:15:11Z

New page: '''Unreleased structure''' The entry 4e2v is ON HOLD Authors: Schmidt, I., Reuter, K., Klebe, G., Heine, A., Tidten, N. Description: tRNA-guanine transglycosylase Y106F, C158V mutant i...

New page

'''Unreleased structure'''

The entry 4e2v is ON HOLD

Authors: Schmidt, I., Reuter, K., Klebe, G., Heine, A., Tidten, N.

Description: tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1