4e51 - Revision history

OCA at 14:52, 14 March 2024

2024-03-14T14:52:39Z

← Older revision		Revision as of 14:52, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_thailandensis_E264 Burkholderia thailandensis E264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E51 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_thailandensis_E264 Burkholderia thailandensis E264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E51 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [https://pdbe.org/4e51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [https://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [https://pdbe.org/4e51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [https://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/SYH_BURTA SYH_BURTA]]		[https://www.uniprot.org/uniprot/SYH_BURTA SYH_BURTA]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Aminoacyl-tRNA synthetases (aaRSs) charge tRNAs with their cognate amino acid, an essential precursor step to loading of charged tRNAs onto the ribosome and addition of the amino acid to the growing polypeptide chain during protein synthesis. Because of this important biological function, aminoacyl-tRNA synthetases have been the focus of anti-infective drug development efforts and two aaRS inhibitors have been approved as drugs. Several researchers in the scientific community requested aminoacyl-tRNA synthetases to be targeted in the Seattle Structural Genomics Center for Infectious Disease (SSGCID) structure determination pipeline. Here we investigate thirty-one aminoacyl-tRNA synthetases from infectious disease organisms by co-crystallization in the presence of their cognate amino acid, ATP, and/or inhibitors. Crystal structures were determined for a CysRS from Borrelia burgdorferi bound to AMP, GluRS from Borrelia burgdorferi and Burkholderia thailandensis bound to glutamic acid, a TrpRS from the eukaryotic pathogen Encephalitozoon cuniculi bound to tryptophan, a HisRS from Burkholderia thailandensis bound to histidine, and a LysRS from Burkholderia thailandensis bound to lysine. Thus, the presence of ligands may promote aaRS crystallization and structure determination. Comparison with homologous structures shows conformational flexibility that appears to be a recurring theme with this enzyme class.

	Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms.,Moen SO, Edwards TE, Dranow DM, Clifton MC, Sankaran B, Van Voorhis WC, Sharma A, Manoil C, Staker BL, Myler PJ, Lorimer DD Sci Rep. 2017 Mar 16;7(1):223. doi: 10.1038/s41598-017-00367-6. PMID:28303005<ref>PMID:28303005</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e51" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Aminoacyl tRNA synthetase 3D structures\|Aminoacyl tRNA synthetase 3D structures]]		*[[Aminoacyl tRNA synthetase 3D structures\|Aminoacyl tRNA synthetase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Burkholderia thailandensis E264]]		[[Category: Burkholderia thailandensis E264]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 06:50, 28 September 2022

2022-09-28T06:50:14Z

← Older revision		Revision as of 06:50, 28 September 2022
Line 3:		Line 3:
	<StructureSection load='4e51' size='340' side='right'caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='4e51' size='340' side='right'caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Burta Burta~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E51 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_thailandensis_E264 Burkholderia thailandensis E264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E51 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTH_I2235, hisS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [https://pdbe.org/4e51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [https://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [~~http~~://pdbe.org/4e51 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/SYH_BURTA SYH_BURTA]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Burta]]~~		[[Category: Burkholderia thailandensis E264]]
	~~[[Category: Histidine--tRNA ligase~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Structural genomic]]~~
	~~[[Category: Aar]]~~
	~~[[Category: Aminoacylation]]~~
	~~[[Category: Atp-dependent]]~~
	~~[[Category: Charged trna]]~~
	~~[[Category: Histidyl-adenylate]]~~
	~~[[Category: Ligase]]~~
	~~[[Category: Ssgcid]]~~
	~~[[Category: Trna activation]]~~
	~~[[Category: Trna synthetase]]~~

OCA at 08:00, 27 March 2019

2019-03-27T08:00:34Z

← Older revision		Revision as of 08:00, 27 March 2019
Line 1:		Line 1:

	==Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine==		==Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine==
	<StructureSection load='4e51' size='340' side='right' caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='4e51' size='340' side='right'caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E51 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burta Burta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E51 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTH_I2235, hisS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [http://pdbe.org/4e51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [http://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [http://pdbe.org/4e51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [http://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4e51" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4e51" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Aminoacyl tRNA synthetase 3D structures\|Aminoacyl tRNA synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Burta]]
	[[Category: Histidine--tRNA ligase]]		[[Category: Histidine--tRNA ligase]]
			[[Category: Large Structures]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Aar]]		[[Category: Aar]]

OCA at 06:59, 29 March 2017

2017-03-29T06:59:00Z

← Older revision		Revision as of 06:59, 29 March 2017
Line 3:		Line 3:
	<StructureSection load='4e51' size='340' side='right' caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='4e51' size='340' side='right' caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Burta Burta]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E51 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E51 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTH_I2235, hisS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [http://pdbe.org/4e51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [http://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [http://pdbe.org/4e51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [http://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>
Line 11:		Line 10:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	~~BACKGROUND: The genus Burkholderia includes pathogenic gram~~-~~negative bacteria that cause melioidosis~~, ~~glanders, and pulmonary infections~~ of ~~patients with cancer~~ and ~~cystic fibrosis. Drug resistance has made development~~ of new antimicrobials critical. Many approaches to discovering new antimicrobials, such as structure-based drug design and whole cell phenotypic screens followed by lead refinement, require high-resolution structures of proteins essential to the ~~parasite~~. ~~METHODOLOGY/PRINCIPAL FINDINGS: We experimentally identified 406 putative essential genes in B. thailandensis~~, ~~a low~~-~~virulence species phylogenetically similar to B. pseudomallei,~~ the ~~causative agent~~ of ~~melioidosis, using saturation~~-~~level transposon mutagenesis~~ and ~~next-generation sequencing (Tn-seq)~~. ~~We selected 315 protein products of these genes based on structure~~-~~determination criteria, such as excluding very large and/or integral membrane proteins, and entered them into~~ the Seattle Structural Genomics Center for ~~Infection~~ Disease (SSGCID) structure determination pipeline. ~~To maximize structural coverage of these targets,~~ we ~~applied an "ortholog rescue" strategy for those producing insoluble or difficult to crystallize proteins, resulting~~ in the ~~addition~~ of ~~387 orthologs (~~or ~~paralogs) from seven other Burkholderia species into the SSGCID pipeline~~. ~~This structural genomics approach yielded~~ structures from ~~31 putative essential targets~~ from ~~B. thailandensis,~~ and ~~25 orthologs from other~~ Burkholderia ~~species, yielding an overall structural coverage for 49 of the 406 essential gene families~~, ~~with~~ a ~~total of 88 depositions into~~ the ~~Protein Data Bank. Of these~~, ~~25 proteins have properties of~~ a ~~potential antimicrobial drug target i.e., no close human homolog, part of an essential metabolic pathway~~, and a ~~deep binding pocket~~. ~~We describe~~ the ~~structures~~ of ~~several potential drug targets in detail~~. ~~CONCLUSIONS/SIGNIFICANCE: This collection of~~ structures~~, solubility and experimental essentiality data provides~~ a ~~resource for development of drugs against infections and diseases caused by Burkholderia. All expression clones and proteins created in~~ this ~~study are freely available by request~~.		Aminoacyl-tRNA synthetases (aaRSs) charge tRNAs with their cognate amino acid, an essential precursor step to loading of charged tRNAs onto the ribosome and addition of the amino acid to the growing polypeptide chain during protein synthesis. Because of this important biological function, aminoacyl-tRNA synthetases have been the focus of anti-infective drug development efforts and two aaRS inhibitors have been approved as drugs. Several researchers in the scientific community requested aminoacyl-tRNA synthetases to be targeted in the Seattle Structural Genomics Center for Infectious Disease (SSGCID) structure determination pipeline. Here we investigate thirty-one aminoacyl-tRNA synthetases from infectious disease organisms by co-crystallization in the presence of their cognate amino acid, ATP, and/or inhibitors. Crystal structures were determined for a CysRS from Borrelia burgdorferi bound to AMP, GluRS from Borrelia burgdorferi and Burkholderia thailandensis bound to glutamic acid, a TrpRS from the eukaryotic pathogen Encephalitozoon cuniculi bound to tryptophan, a HisRS from Burkholderia thailandensis bound to histidine, and a LysRS from Burkholderia thailandensis bound to lysine. Thus, the presence of ligands may promote aaRS crystallization and structure determination. Comparison with homologous structures shows conformational flexibility that appears to be a recurring theme with this enzyme class.

	~~Combining functional and structural genomics to sample the essential Burkholderia structome~~.,~~Baugh L~~, ~~Gallagher LA~~, ~~Patrapuvich R~~, Clifton MC, ~~Gardberg AS, Edwards TE, Armour~~ B, ~~Begley DW~~, ~~Dieterich SH~~, ~~Dranow DM, Abendroth J, Fairman JW, Fox D 3rd~~, Staker BL~~, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R~~, Myler PJ, ~~Stewart LJ, Manoil C, Van Voorhis WC PLoS One~~. ~~2013~~;8(1):~~e53851~~. doi: 10.~~1371~~/~~journal.pone.0053851. Epub 2013 Jan 31~~. PMID:~~23382856~~<ref>PMID:~~23382856~~</ref>		Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms.,Moen SO, Edwards TE, Dranow DM, Clifton MC, Sankaran B, Van Voorhis WC, Sharma A, Manoil C, Staker BL, Myler PJ, Lorimer DD Sci Rep. 2017 Mar 16;7(1):223. doi: 10.1038/s41598-017-00367-6. PMID:28303005<ref>PMID:28303005</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Burta]]~~
	[[Category: Histidine--tRNA ligase]]		[[Category: Histidine--tRNA ligase]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 06:04, 5 August 2016

2016-08-05T06:04:11Z

← Older revision		Revision as of 06:04, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine==		==Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine==
	<StructureSection load='4e51' size='340' side='right' caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='4e51' size='340' side='right' caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Burkholderia_thailandensis_e264 Burkholderia thailandensis e264~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E51 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burta Burta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E51 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTH_I2235, hisS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 ~~Burkholderia thailandensis E264~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTH_I2235, hisS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [http://www.ebi.ac.uk/pdbsum/4e51 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [http://pdbe.org/4e51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [http://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e51" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Burkholderia thailandensis e264~~]]		[[Category: Burta]]
	[[Category: Histidine--tRNA ligase]]		[[Category: Histidine--tRNA ligase]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 11:29, 21 December 2014

2014-12-21T11:29:18Z

← Older revision		Revision as of 11:29, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4e51\| PDB=4e51 \| SCENE= }}~~		==Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine==
	===Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine===		<StructureSection load='4e51' size='340' side='right' caption='[[4e51]], [[Resolution\|resolution]] 2.65Å' scene=''>
	~~{{ABSTRACT_PUBMED_23382856}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis_e264 Burkholderia thailandensis e264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E51 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTH_I2235, hisS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 Burkholderia thailandensis E264])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [http://www.ebi.ac.uk/pdbsum/4e51 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			BACKGROUND: The genus Burkholderia includes pathogenic gram-negative bacteria that cause melioidosis, glanders, and pulmonary infections of patients with cancer and cystic fibrosis. Drug resistance has made development of new antimicrobials critical. Many approaches to discovering new antimicrobials, such as structure-based drug design and whole cell phenotypic screens followed by lead refinement, require high-resolution structures of proteins essential to the parasite. METHODOLOGY/PRINCIPAL FINDINGS: We experimentally identified 406 putative essential genes in B. thailandensis, a low-virulence species phylogenetically similar to B. pseudomallei, the causative agent of melioidosis, using saturation-level transposon mutagenesis and next-generation sequencing (Tn-seq). We selected 315 protein products of these genes based on structure-determination criteria, such as excluding very large and/or integral membrane proteins, and entered them into the Seattle Structural Genomics Center for Infection Disease (SSGCID) structure determination pipeline. To maximize structural coverage of these targets, we applied an "ortholog rescue" strategy for those producing insoluble or difficult to crystallize proteins, resulting in the addition of 387 orthologs (or paralogs) from seven other Burkholderia species into the SSGCID pipeline. This structural genomics approach yielded structures from 31 putative essential targets from B. thailandensis, and 25 orthologs from other Burkholderia species, yielding an overall structural coverage for 49 of the 406 essential gene families, with a total of 88 depositions into the Protein Data Bank. Of these, 25 proteins have properties of a potential antimicrobial drug target i.e., no close human homolog, part of an essential metabolic pathway, and a deep binding pocket. We describe the structures of several potential drug targets in detail. CONCLUSIONS/SIGNIFICANCE: This collection of structures, solubility and experimental essentiality data provides a resource for development of drugs against infections and diseases caused by Burkholderia. All expression clones and proteins created in this study are freely available by request.

	~~==About this Structure==~~		Combining functional and structural genomics to sample the essential Burkholderia structome.,Baugh L, Gallagher LA, Patrapuvich R, Clifton MC, Gardberg AS, Edwards TE, Armour B, Begley DW, Dieterich SH, Dranow DM, Abendroth J, Fairman JW, Fox D 3rd, Staker BL, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R, Myler PJ, Stewart LJ, Manoil C, Van Voorhis WC PLoS One. 2013;8(1):e53851. doi: 10.1371/journal.pone.0053851. Epub 2013 Jan 31. PMID:23382856<ref>PMID:23382856</ref>
	~~[[4e51]] is a 2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki~~/~~Burkholderia_thailandensis_e264 Burkholderia thailandensis e264]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~ocashort?id=4E51 OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:023382856</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Burkholderia thailandensis e264]]		[[Category: Burkholderia thailandensis e264]]
	[[Category: Histidine--tRNA ligase]]		[[Category: Histidine--tRNA ligase]]
	[[Category: ~~SSGCID, Seattle~~ Structural ~~Genomics Center for Infectious Disease.~~]]		[[Category: Structural genomic]]
	[[Category: Aar]]		[[Category: Aar]]
	[[Category: Aminoacylation]]		[[Category: Aminoacylation]]
Line 17:		Line 29:
	[[Category: Histidyl-adenylate]]		[[Category: Histidyl-adenylate]]
	[[Category: Ligase]]		[[Category: Ligase]]
	~~[[Category: Seattle structural genomics center for infectious disease]]~~
	[[Category: Ssgcid]]		[[Category: Ssgcid]]
	[[Category: Trna activation]]		[[Category: Trna activation]]
	[[Category: Trna synthetase]]		[[Category: Trna synthetase]]

OCA at 08:21, 30 October 2013

2013-10-30T08:21:01Z

← Older revision		Revision as of 08:21, 30 October 2013
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	~~[[Image:4e51.jpg\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_4e51", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_4e51\| PDB=4e51 \| SCENE= }}		{{STRUCTURE_4e51\| PDB=4e51 \| SCENE= }}

	===Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine===		===Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine===
			{{ABSTRACT_PUBMED_23382856}}

			==About this Structure==
			[[4e51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis_e264 Burkholderia thailandensis e264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA].

	==~~About this Structure~~==		==Reference==
	~~[[4e51]] is a 2 chain structure with sequence from [http~~://~~en.wikipedia.org/wiki~~/~~Burkholderia_thailandensis Burkholderia thailandensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA].~~		<ref group="xtra">PMID:023382856</ref><references group="xtra"/><references/>
	[[Category: Burkholderia thailandensis]]		[[Category: Burkholderia thailandensis e264]]
	[[Category: Histidine--tRNA ligase]]		[[Category: Histidine--tRNA ligase]]
	[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]		[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]

OCA at 07:55, 28 March 2012

2012-03-28T07:55:31Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4e51.jpg|left|200px]]
-The entry 4e51 is ON HOLD
+<!--
-Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID)
+===Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine===
-Description: Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine

OCA: Protected "4e51" [edit=sysop:move=sysop]

2012-03-21T07:17:28Z

Protected "4e51" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:17, 21 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e51 is ON HOLD Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID) Description: Crystal structure of a histidyl-tRNA synth...

2012-03-21T07:17:27Z

New page: '''Unreleased structure''' The entry 4e51 is ON HOLD Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID) Description: Crystal structure of a histidyl-tRNA synth...

New page

'''Unreleased structure'''

The entry 4e51 is ON HOLD

Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Description: Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine