4e6k - Revision history

OCA at 10:50, 13 August 2026

2026-08-13T10:50:00Z

← Older revision		Revision as of 10:50, 13 August 2026
Line 9:		Line 9:
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/~~Q9HY79_PSEAE Q9HY79_PSEAE~~] ~~Iron~~-storage protein (~~By similarity~~). ~~Iron-storage protein, whose~~ ferroxidase center binds Fe(2+) ~~ions~~, oxidizes ~~them by~~ dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (~~By similarity~~).[~~PIRNR~~:~~PIRNR002560]~~		[https://www.uniprot.org/uniprot/BFRB_PSEAE BFRB_PSEAE] The major iron-storage protein, part of the heterooligomeric bacterioferritin (BFR) complex. The ferroxidase center binds Fe(2+), oxidizes it using dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the BFR protein shell. Can store up to 600 iron atoms per bacterioferritin protein molecule (PubMed:19575528, PubMed:20067302, PubMed:25640193, PubMed:26368531). In iron-sufficient conditions (10 uM Fe(2+)) iron accumulates in BFR until about 12 hours, when it starts to deplete; stored iron is no longer detectable by 24 hours growth, iron is mobilized from the BFR as levels drop in the growth media (PubMed:28318006). Iron release from the BFR requires ferredoxin NADP reductase (FPR) and bacterioferritin-associated ferredoxin (Bfd) (PubMed:19575528, PubMed:22812654, PubMed:26368531). Reduction of the BfrB heme group occurs in the presence of Bfd, strongly suggesting that the BfrB-Bfd complex allows heme to mediate electron transfer from FPR to the Fe(3+) iron core in the BFR shell prior to its release as Fe(2+) (PubMed:19575528, PubMed:22812654, PubMed:26368531).<ref>PMID:19575528</ref> <ref>PMID:20067302</ref> <ref>PMID:22812654</ref> <ref>PMID:25640193</ref> <ref>PMID:26368531</ref> <ref>PMID:28318006</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Ferritin-like molecules are unique to cellular iron homeostasis because they can store iron at concentrations much higher than those dictated by the solubility of Fe(3+). Very little is known about the protein interactions that deliver iron for storage, or promote the mobilization of stored iron from ferritin-like molecules. Here, we report the X-ray crystal structure of Pseudomonas aeruginosa bacterioferritin (Pa-BfrB) in complex with bacterioferritin-associated ferredoxin (Pa-Bfd) at 2.0 A resolution. As the first example of a ferritin-like molecule in complex with a cognate partner, the structure provides unprecedented insight into the complementary interface that enables the [2Fe-2S] cluster of Pa-Bfd to promote heme-mediated electron transfer through the BfrB protein dielectric (~18 A), a process that is necessary to reduce the core ferric mineral and facilitate mobilization of Fe(2+). The Pa-BfrB-Bfd complex also revealed the first structure of a Bfd, thus providing a first view to what appears to be a versatile metal binding domain ubiquitous to the large Fer2_BFD family of proteins and enzymes with diverse functions. Residues at the Pa-BfrB-Bfd interface are highly conserved in Bfr and Bfd sequences from a number of pathogenic bacteria, suggesting that the specific recognition between Pa-BfrB and Pa-Bfd is of widespread significance to the understanding of bacterial iron homeostasis.

			The structure of the BfrB-Bfd complex reveals protein-protein interactions enabling iron release from bacterioferritin.,Yao H, Wang Y, Lovell SW, Kumar R, Ruvinsky AM, Battaile KP, Vakser IA, Rivera M J Am Chem Soc. 2012 Jul 19. PMID:22812654<ref>PMID:22812654</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4e6k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ferritin 3D structures\|Ferritin 3D structures]]		*[[Ferritin 3D structures\|Ferritin 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:53, 14 March 2024

2024-03-14T14:53:44Z

← Older revision		Revision as of 14:53, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E6K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E6K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [https://pdbe.org/4e6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [https://www.ebi.ac.uk/pdbsum/4e6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [https://pdbe.org/4e6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [https://www.ebi.ac.uk/pdbsum/4e6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE]] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]		[https://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Ferritin-like molecules are unique to cellular iron homeostasis because they can store iron at concentrations much higher than those dictated by the solubility of Fe(3+). Very little is known about the protein interactions that deliver iron for storage, or promote the mobilization of stored iron from ferritin-like molecules. Here, we report the X-ray crystal structure of Pseudomonas aeruginosa bacterioferritin (Pa-BfrB) in complex with bacterioferritin-associated ferredoxin (Pa-Bfd) at 2.0 A resolution. As the first example of a ferritin-like molecule in complex with a cognate partner, the structure provides unprecedented insight into the complementary interface that enables the [2Fe-2S] cluster of Pa-Bfd to promote heme-mediated electron transfer through the BfrB protein dielectric (~18 A), a process that is necessary to reduce the core ferric mineral and facilitate mobilization of Fe(2+). The Pa-BfrB-Bfd complex also revealed the first structure of a Bfd, thus providing a first view to what appears to be a versatile metal binding domain ubiquitous to the large Fer2_BFD family of proteins and enzymes with diverse functions. Residues at the Pa-BfrB-Bfd interface are highly conserved in Bfr and Bfd sequences from a number of pathogenic bacteria, suggesting that the specific recognition between Pa-BfrB and Pa-Bfd is of widespread significance to the understanding of bacterial iron homeostasis.

	The structure of the BfrB-Bfd complex reveals protein-protein interactions enabling iron release from bacterioferritin.,Yao H, Wang Y, Lovell SW, Kumar R, Ruvinsky AM, Battaile KP, Vakser IA, Rivera M J Am Chem Soc. 2012 Jul 19. PMID:22812654<ref>PMID:22812654</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e6k" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Ferritin 3D structures\|Ferritin 3D structures]]		*[[Ferritin 3D structures\|Ferritin 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:53, 28 September 2022

2022-09-28T06:53:44Z

← Older revision		Revision as of 06:53, 28 September 2022
Line 1:		Line 1:

	==2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)==		==2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)==
	<StructureSection load='4e6k' size='340' side='right' caption='[[4e6k]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4e6k' size='340' side='right'caption='[[4e6k]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E6K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E6K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), BFD, PA3530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [https://pdbe.org/4e6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [https://www.ebi.ac.uk/pdbsum/4e6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6k ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [~~http~~://pdbe.org/4e6k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e6k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE]] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]		[[https://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE]] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4e6k" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4e6k" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Ferritin 3D structures\|Ferritin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Pseae~~]]		[[Category: Large Structures]]
	[[Category: Battaile~~, K P~~]]		[[Category: Pseudomonas aeruginosa PAO1]]
	[[Category: Kumar, R]]		[[Category: Battaile KP]]
	[[Category: Lovell, S]]		[[Category: Kumar R]]
	[[Category: Rivera, M]]		[[Category: Lovell S]]
	[[Category: Ruvinsky, A]]		[[Category: Rivera M]]
	[[Category: Vasker, I]]		[[Category: Ruvinsky A]]
	[[Category: Wang, Y]]		[[Category: Vasker I]]
	[[Category: Yao, H]]		[[Category: Wang Y]]
	~~[[Category: Bacterial iron metabolism]]~~		[[Category: Yao H]]
	~~[[Category: Ferritin]]~~
	~~[[Category: Iron binding]]~~
	~~[[Category: Iron homeostasis]]~~
	~~[[Category: Iron mobilization]]~~
	~~[[Category: Iron storage]]~~
	~~[[Category: Metal binding protein-electron transport complex]]~~
	~~[[Category: Protein complex~~]]

OCA at 12:15, 4 August 2016

2016-08-04T12:15:52Z

← Older revision		Revision as of 12:15, 4 August 2016
Line 1:		Line 1:

	==2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)==		==2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)==
	<StructureSection load='4e6k' size='340' side='right' caption='[[4e6k]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4e6k' size='340' side='right' caption='[[4e6k]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E6K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E6K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~287 Pseudomonas aeruginosa~~]), BFD, PA3530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~287 Pseudomonas aeruginosa~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), BFD, PA3530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [http://www.ebi.ac.uk/pdbsum/4e6k PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [http://pdbe.org/4e6k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [http://www.ebi.ac.uk/pdbsum/4e6k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e6k" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Pseudomonas aeruginosa~~]]		[[Category: Pseae]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Kumar, R]]		[[Category: Kumar, R]]

OCA at 14:23, 24 December 2014

2014-12-24T14:23:09Z

← Older revision		Revision as of 14:23, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E6K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E6K FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]), BFD, PA3530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]), BFD, PA3530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [http://www.ebi.ac.uk/pdbsum/4e6k PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [http://www.ebi.ac.uk/pdbsum/4e6k PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE]] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Battaile, K P.]]		[[Category: Battaile, K P]]
	[[Category: Kumar, R.]]		[[Category: Kumar, R]]
	[[Category: Lovell, S.]]		[[Category: Lovell, S]]
	[[Category: Rivera, M.]]		[[Category: Rivera, M]]
	[[Category: Ruvinsky, A.]]		[[Category: Ruvinsky, A]]
	[[Category: Vasker, I.]]		[[Category: Vasker, I]]
	[[Category: Wang, Y.]]		[[Category: Wang, Y]]
	[[Category: Yao, H.]]		[[Category: Yao, H]]
	[[Category: Bacterial iron metabolism]]		[[Category: Bacterial iron metabolism]]
	[[Category: Ferritin]]		[[Category: Ferritin]]

OCA at 09:14, 11 June 2014

2014-06-11T09:14:39Z

← Older revision		Revision as of 09:14, 11 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~4e6k~~.~~png~~\|~~left~~\|~~200px~~]]		==2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)==
			<StructureSection load='4e6k' size='340' side='right' caption='[[4e6k]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4e6k]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E6K FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]), BFD, PA3530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e6k RCSB], [http://www.ebi.ac.uk/pdbsum/4e6k PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Ferritin-like molecules are unique to cellular iron homeostasis because they can store iron at concentrations much higher than those dictated by the solubility of Fe(3+). Very little is known about the protein interactions that deliver iron for storage, or promote the mobilization of stored iron from ferritin-like molecules. Here, we report the X-ray crystal structure of Pseudomonas aeruginosa bacterioferritin (Pa-BfrB) in complex with bacterioferritin-associated ferredoxin (Pa-Bfd) at 2.0 A resolution. As the first example of a ferritin-like molecule in complex with a cognate partner, the structure provides unprecedented insight into the complementary interface that enables the [2Fe-2S] cluster of Pa-Bfd to promote heme-mediated electron transfer through the BfrB protein dielectric (~18 A), a process that is necessary to reduce the core ferric mineral and facilitate mobilization of Fe(2+). The Pa-BfrB-Bfd complex also revealed the first structure of a Bfd, thus providing a first view to what appears to be a versatile metal binding domain ubiquitous to the large Fer2_BFD family of proteins and enzymes with diverse functions. Residues at the Pa-BfrB-Bfd interface are highly conserved in Bfr and Bfd sequences from a number of pathogenic bacteria, suggesting that the specific recognition between Pa-BfrB and Pa-Bfd is of widespread significance to the understanding of bacterial iron homeostasis.

	~~{{STRUCTURE_4e6k\| PDB=4e6k \| SCENE= }}~~		The structure of the BfrB-Bfd complex reveals protein-protein interactions enabling iron release from bacterioferritin.,Yao H, Wang Y, Lovell SW, Kumar R, Ruvinsky AM, Battaile KP, Vakser IA, Rivera M J Am Chem Soc. 2012 Jul 19. PMID:22812654<ref>PMID:22812654</ref>

	~~===2.0 A resolution structure~~ of ~~Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22812654}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[4e6k]] is a 9 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4E6K OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:022812654<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Battaile, K P.]]		[[Category: Battaile, K P.]]

OCA at 05:38, 29 August 2012

2012-08-29T05:38:06Z

← Older revision		Revision as of 05:38, 29 August 2012
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	[[Image:4e6k.~~jpg~~\|left\|200px]]		[[Image:4e6k.png\|left\|200px]]

	{{STRUCTURE_4e6k\| PDB=4e6k \| SCENE= }}		{{STRUCTURE_4e6k\| PDB=4e6k \| SCENE= }}

OCA at 05:52, 1 August 2012

2012-08-01T05:52:20Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4e6k.jpg|left|200px]]
-The entry 4e6k is ON HOLD  until Paper Publication
+{{STRUCTURE_4e6k|  PDB=4e6k  |  SCENE=  }}
-Authors: Lovell, S., Battaile, K.P., Yao, H., Wang, Y., Kumar, R., Ruvinsky, A., Vasker, I., Rivera, M.
+===2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)===
-Description: 2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)
+{{ABSTRACT_PUBMED_22812654}}

OCA at 05:45, 4 April 2012

2012-04-04T05:45:45Z

← Older revision		Revision as of 05:45, 4 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4e6k is ON HOLD		The entry 4e6k is ON HOLD until Paper Publication

	Authors: Lovell, S., Battaile, K.P., Yao, H., Wang, Y., Kumar, R., Ruvinsky, A., Vasker, I., Rivera, M.		Authors: Lovell, S., Battaile, K.P., Yao, H., Wang, Y., Kumar, R., Ruvinsky, A., Vasker, I., Rivera, M.

	Description: 2.0A resolution structure of Pseudomonas BfrB in complex with Bfd		Description: 2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd)

OCA at 07:22, 28 March 2012

2012-03-28T07:22:39Z

← Older revision		Revision as of 07:22, 28 March 2012
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	The entry 4e6k is ON HOLD		The entry 4e6k is ON HOLD

	Authors: Lovell, ~~Scott~~, Battaile, ~~Kevin~~ P., Yao, ~~Huili~~, Wang, ~~Yan~~, Kumar, ~~Ritesh~~, Ruvinsky, ~~Anatoly~~, Vasker, ~~Ilya~~, Rivera, ~~Mario~~		Authors: Lovell, S., Battaile, K.P., Yao, H., Wang, Y., Kumar, R., Ruvinsky, A., Vasker, I., Rivera, M.

	Description: 2.0A resolution structure of Pseudomonas BfrB in complex with Bfd		Description: 2.0A resolution structure of Pseudomonas BfrB in complex with Bfd