4e70 - Revision history

OCA at 11:02, 1 March 2024

2024-03-01T11:02:17Z

← Older revision		Revision as of 11:02, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Linum_nodiflorum Linum nodiflorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E70 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Linum_nodiflorum Linum nodiflorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E70 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N7I:4-[(1E)-3-HYDROXYPROP-1-EN-1-YL]-2-METHOXYPHENOL'>N7I</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6093Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N7I:4-[(1E)-3-HYDROXYPROP-1-EN-1-YL]-2-METHOXYPHENOL'>N7I</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e70 OCA], [https://pdbe.org/4e70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e70 RCSB], [https://www.ebi.ac.uk/pdbsum/4e70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e70 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e70 OCA], [https://pdbe.org/4e70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e70 RCSB], [https://www.ebi.ac.uk/pdbsum/4e70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e70 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/A6XNE6_9ROSI A6XNE6_9ROSI]]		[https://www.uniprot.org/uniprot/A6XNE6_9ROSI A6XNE6_9ROSI]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Coniferyl alcohol 9-O-methyltransferase from Linum nodiflorum (Linaceae) catalyzes the unusual methylation of the side-chain hydroxyl group of coniferyl alcohol. The protein was heterologously expressed in Escherichia coli as a hexahistidine derivative and purified for crystallization. Diffracting crystals were obtained of the pure protein and of its selenomethionine derivative, as well as of complexes with coniferyl alcohol and with S-adenosyl-L-homocysteine together with coniferyl alcohol 9-O-methyl ether (PDB entries 4ems, 4e70 and 4evi, respectively). The X-ray structures show that the phenylpropanoid binding mode differs from other phenylpropanoid O-methyltransferases such as caffeic acid O-methyltransferase. Moreover, the active site lacks the usually conserved and catalytic histidine residue and thus implies a different reaction mode for methylation. Site-directed mutagenesis was carried out to identify critical amino acids. The binding order of coniferyl alcohol and S-adenosyl-L-methionine was investigated by isothermal titration calorimetry experiments.

	Structural analysis of coniferyl alcohol 9-O-methyltransferase from Linum nodiflorum reveals a novel active-site environment.,Wolters S, Neeb M, Berim A, Schulze Wischeler J, Petersen M, Heine A Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):888-900. doi:, 10.1107/S0907444913002874. Epub 2013 Apr 19. PMID:23633600<ref>PMID:23633600</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e70" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:54, 28 September 2022

2022-09-28T06:54:32Z

← Older revision		Revision as of 06:54, 28 September 2022
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	==Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol==		==Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol==
	<StructureSection load='4e70' size='340' side='right' caption='[[4e70]], [[Resolution\|resolution]] 1.61Å' scene=''>		<StructureSection load='4e70' size='340' side='right'caption='[[4e70]], [[Resolution\|resolution]] 1.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e70]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Linum_nodiflorum Linum nodiflorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E70 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E70 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Linum_nodiflorum Linum nodiflorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E70 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N7I:4-[(1E)-3-HYDROXYPROP-1-EN-1-YL]-2-METHOXYPHENOL'>N7I</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N7I:4-[(1E)-3-HYDROXYPROP-1-EN-1-YL]-2-METHOXYPHENOL'>N7I</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CA9OMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=407264 Linum nodiflorum])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e70 OCA], [https://pdbe.org/4e70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e70 RCSB], [https://www.ebi.ac.uk/pdbsum/4e70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e70 ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e70 OCA], [~~http~~://pdbe.org/4e70 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e70 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e70 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e70 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/A6XNE6_9ROSI A6XNE6_9ROSI]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Linum nodiflorum]]		[[Category: Linum nodiflorum]]
	[[Category: Heine, A]]		[[Category: Heine A]]
	[[Category: Petersen, M]]		[[Category: Petersen M]]
	[[Category: Wolters, S]]		[[Category: Wolters S]]
	~~[[Category: Coniferyl alcohol]]~~
	~~[[Category: Dimer]]~~
	~~[[Category: Rossmann fold]]~~
	~~[[Category: S-adenosyl-l-methionine]]~~
	~~[[Category: Small molecule o-methyltransferase]]~~
	~~[[Category: Transferase~~]]

OCA at 14:32, 11 August 2016

2016-08-11T14:32:30Z

← Older revision		Revision as of 14:32, 11 August 2016
Line 1:		Line 1:

	==Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol==		==Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol==
	<StructureSection load='4e70' size='340' side='right' caption='[[4e70]], [[Resolution\|resolution]] 1.61Å' scene=''>		<StructureSection load='4e70' size='340' side='right' caption='[[4e70]], [[Resolution\|resolution]] 1.61Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N7I:4-[(1E)-3-HYDROXYPROP-1-EN-1-YL]-2-METHOXYPHENOL'>N7I</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N7I:4-[(1E)-3-HYDROXYPROP-1-EN-1-YL]-2-METHOXYPHENOL'>N7I</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CA9OMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=407264 Linum nodiflorum])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CA9OMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=407264 Linum nodiflorum])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e70 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e70 RCSB], [http://www.ebi.ac.uk/pdbsum/4e70 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e70 OCA], [http://pdbe.org/4e70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e70 RCSB], [http://www.ebi.ac.uk/pdbsum/4e70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e70 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e70" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:59, 27 January 2015

2015-01-27T04:59:10Z

← Older revision		Revision as of 04:59, 27 January 2015
Line 1:		Line 1:
	~~{{STRUCTURE_4e70\| PDB=4e70 \| SCENE= }}~~		==Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol==
	===Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol===		<StructureSection load='4e70' size='340' side='right' caption='[[4e70]], [[Resolution\|resolution]] 1.61Å' scene=''>
	~~{{ABSTRACT_PUBMED_23633600}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4e70]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Linum_nodiflorum Linum nodiflorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E70 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N7I:4-[(1E)-3-HYDROXYPROP-1-EN-1-YL]-2-METHOXYPHENOL'>N7I</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CA9OMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=407264 Linum nodiflorum])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e70 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e70 RCSB], [http://www.ebi.ac.uk/pdbsum/4e70 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Coniferyl alcohol 9-O-methyltransferase from Linum nodiflorum (Linaceae) catalyzes the unusual methylation of the side-chain hydroxyl group of coniferyl alcohol. The protein was heterologously expressed in Escherichia coli as a hexahistidine derivative and purified for crystallization. Diffracting crystals were obtained of the pure protein and of its selenomethionine derivative, as well as of complexes with coniferyl alcohol and with S-adenosyl-L-homocysteine together with coniferyl alcohol 9-O-methyl ether (PDB entries 4ems, 4e70 and 4evi, respectively). The X-ray structures show that the phenylpropanoid binding mode differs from other phenylpropanoid O-methyltransferases such as caffeic acid O-methyltransferase. Moreover, the active site lacks the usually conserved and catalytic histidine residue and thus implies a different reaction mode for methylation. Site-directed mutagenesis was carried out to identify critical amino acids. The binding order of coniferyl alcohol and S-adenosyl-L-methionine was investigated by isothermal titration calorimetry experiments.

	~~==About this Structure==~~		Structural analysis of coniferyl alcohol 9-O-methyltransferase from Linum nodiflorum reveals a novel active-site environment.,Wolters S, Neeb M, Berim A, Schulze Wischeler J, Petersen M, Heine A Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):888-900. doi:, 10.1107/S0907444913002874. Epub 2013 Apr 19. PMID:23633600<ref>PMID:23633600</ref>
	~~[[4e70]] is~~ a ~~2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Linum_nodiflorum Linum nodiflorum]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin~~/~~ocashort?id=4E70 OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Linum nodiflorum]]		[[Category: Linum nodiflorum]]
	[[Category: Heine, A.]]		[[Category: Heine, A]]
	[[Category: Petersen, M.]]		[[Category: Petersen, M]]
	[[Category: Wolters, S.]]		[[Category: Wolters, S]]
	[[Category: Coniferyl alcohol]]		[[Category: Coniferyl alcohol]]
	[[Category: Dimer]]		[[Category: Dimer]]

OCA at 06:29, 16 May 2013

2013-05-16T06:29:48Z

← Older revision		Revision as of 06:29, 16 May 2013
Line 1:		Line 1:
	{{STRUCTURE_4e70\| PDB=4e70 \| SCENE= }}		{{STRUCTURE_4e70\| PDB=4e70 \| SCENE= }}
	===Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol===		===Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol===
			{{ABSTRACT_PUBMED_23633600}}

	==About this Structure==		==About this Structure==

OCA at 07:46, 2 May 2013

2013-05-02T07:46:23Z

← Older revision		Revision as of 07:46, 2 May 2013
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		{{STRUCTURE_4e70\| PDB=4e70 \| SCENE= }}
			===Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol===

	~~The entry~~ 4e70 is ~~ON HOLD until Paper Publication~~		==About this Structure==
			[[4e70]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Linum_nodiflorum Linum nodiflorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E70 OCA].
	~~Authors~~: ~~Wolters, S.,~~ Heine, A., Petersen, M.		[[Category: Linum nodiflorum]]
			[[Category: Heine, A.]]
	~~Description~~: ~~Crystal Structure Analysis of~~ Coniferyl ~~Alcohol 9~~-O-~~Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol~~		[[Category: Petersen, M.]]
			[[Category: Wolters, S.]]
			[[Category: Coniferyl alcohol]]
			[[Category: Dimer]]
			[[Category: Rossmann fold]]
			[[Category: S-adenosyl-l-methionine]]
			[[Category: Small molecule o-methyltransferase]]
			[[Category: Transferase]]

OCA at 05:45, 4 April 2012

2012-04-04T05:45:52Z

← Older revision		Revision as of 05:45, 4 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4e70 is ON HOLD		The entry 4e70 is ON HOLD until Paper Publication

	Authors: Wolters, S., Heine, A., Petersen, M.		Authors: Wolters, S., Heine, A., Petersen, M.

	Description: Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol		Description: Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol

OCA: Protected "4e70" [edit=sysop:move=sysop]

2012-03-28T07:22:54Z

Protected "4e70" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:22, 28 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e70 is ON HOLD Authors: Wolters, S., Heine, A., Petersen, M. Description: Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase fr...

2012-03-28T07:22:53Z

New page: '''Unreleased structure''' The entry 4e70 is ON HOLD Authors: Wolters, S., Heine, A., Petersen, M. Description: Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase fr...

New page

'''Unreleased structure'''

The entry 4e70 is ON HOLD

Authors: Wolters, S., Heine, A., Petersen, M.

Description: Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol