4e7v - Revision history

OCA at 09:57, 30 October 2024

2024-10-30T09:57:24Z

← Older revision		Revision as of 09:57, 30 October 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.		[https://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The crystal structures of three conformations, T(6), T(3)R(3) and R(6), of bovine insulin were solved at 1.40, 1.30 and 1.80 A resolution, respectively. All conformations crystallized in space group R3. In contrast to the T(6) and T(3)R(3) structures, different conformations of the N-terminal B-chain residue PheB1 were observed in the R(6) insulin structure, resulting in an eightfold doubling of the unit-cell volume upon cooling. The zinc coordination in each conformation was studied by X-ray absorption spectroscopy (XAS), including both EXAFS and XANES. Zinc adopts a tetrahedral coordination in all R(3) sites and an octahedral coordination in T(3) sites. The coordination distances were refined from XAS with a standard deviation of <0.01 A. In contrast to the distances determined from the medium-resolution crystal structures, the XAS results were in good agreement with similar coordination geometries found in small molecules, as well as in other high-resolution insulin structures. As the radiation dose for XRD experiments is two orders of magnitude higher compared with that of XAS experiments, the single crystals were exposed to a higher degree of radiation damage that affected the zinc coordination in the T(3) sites in particular. Furthermore, XANES spectra for the zinc sites in T(6) and R(6) insulin were successfully calculated using finite difference methods and the bond distances and angles were optimized from a quantitative XANES analysis.

			The structures of T(6), T(3)R(3) and R(6) bovine insulin: combining X-ray diffraction and absorption spectroscopy.,Frankaer CG, Knudsen MV, Noren K, Nazarenko E, Stahl K, Harris P Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1259-71. Epub 2012 Sep, 13. PMID:22993080<ref>PMID:22993080</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4e7v" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Insulin 3D Structures\|Insulin 3D Structures]]		*[[Insulin 3D Structures\|Insulin 3D Structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:54, 14 March 2024

2024-03-14T14:54:57Z

← Older revision		Revision as of 14:54, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E7V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [https://pdbe.org/4e7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [https://www.ebi.ac.uk/pdbsum/4e7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e7v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [https://pdbe.org/4e7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [https://www.ebi.ac.uk/pdbsum/4e7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e7v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.		[https://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The crystal structures of three conformations, T(6), T(3)R(3) and R(6), of bovine insulin were solved at 1.40, 1.30 and 1.80 A resolution, respectively. All conformations crystallized in space group R3. In contrast to the T(6) and T(3)R(3) structures, different conformations of the N-terminal B-chain residue PheB1 were observed in the R(6) insulin structure, resulting in an eightfold doubling of the unit-cell volume upon cooling. The zinc coordination in each conformation was studied by X-ray absorption spectroscopy (XAS), including both EXAFS and XANES. Zinc adopts a tetrahedral coordination in all R(3) sites and an octahedral coordination in T(3) sites. The coordination distances were refined from XAS with a standard deviation of <0.01 A. In contrast to the distances determined from the medium-resolution crystal structures, the XAS results were in good agreement with similar coordination geometries found in small molecules, as well as in other high-resolution insulin structures. As the radiation dose for XRD experiments is two orders of magnitude higher compared with that of XAS experiments, the single crystals were exposed to a higher degree of radiation damage that affected the zinc coordination in the T(3) sites in particular. Furthermore, XANES spectra for the zinc sites in T(6) and R(6) insulin were successfully calculated using finite difference methods and the bond distances and angles were optimized from a quantitative XANES analysis.

	The structures of T(6), T(3)R(3) and R(6) bovine insulin: combining X-ray diffraction and absorption spectroscopy.,Frankaer CG, Knudsen MV, Noren K, Nazarenko E, Stahl K, Harris P Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1259-71. Epub 2012 Sep, 13. PMID:22993080<ref>PMID:22993080</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4e7v" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Insulin 3D Structures\|Insulin 3D Structures]]		*[[Insulin 3D Structures\|Insulin 3D Structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:56, 28 September 2022

2022-09-28T06:56:06Z

← Older revision		Revision as of 06:56, 28 September 2022
Line 3:		Line 3:
	<StructureSection load='4e7v' size='340' side='right'caption='[[4e7v]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4e7v' size='340' side='right'caption='[[4e7v]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E7V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e7t\|4e7t]], [[4e7u\|4e7u]]~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [https://pdbe.org/4e7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [https://www.ebi.ac.uk/pdbsum/4e7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e7v ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [~~http~~://pdbe.org/4e7v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e7v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e7v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.		[[https://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 27:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Frankaer~~, C G~~]]		[[Category: Frankaer CG]]
	[[Category: Harris, P]]		[[Category: Harris P]]
	[[Category: Knudsen~~, M V]]~~		[[Category: Knudsen MV]]
	~~[[Category: Hormone]]~~
	~~[[Category: Zinc binding~~]]

OCA at 09:17, 5 February 2020

2020-02-05T09:17:56Z

← Older revision		Revision as of 09:17, 5 February 2020
Line 1:		Line 1:

	==The structure of R6 bovine insulin==		==The structure of R6 bovine insulin==
	<StructureSection load='4e7v' size='340' side='right' caption='[[4e7v]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4e7v' size='340' side='right'caption='[[4e7v]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E7V FirstGlance]. <br>
Line 21:		Line 21:

	==See Also==		==See Also==
	*[[~~Molecular Playground/~~Insulin\|~~Molecular Playground/~~Insulin]]		*[[Insulin 3D Structures\|Insulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
			[[Category: Large Structures]]
	[[Category: Frankaer, C G]]		[[Category: Frankaer, C G]]
	[[Category: Harris, P]]		[[Category: Harris, P]]

OCA at 17:56, 4 August 2016

2016-08-04T17:56:35Z

← Older revision		Revision as of 17:56, 4 August 2016
Line 1:		Line 1:

	==The structure of R6 bovine insulin==		==The structure of R6 bovine insulin==
	<StructureSection load='4e7v' size='340' side='right' caption='[[4e7v]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4e7v' size='340' side='right' caption='[[4e7v]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e7t\|4e7t]], [[4e7u\|4e7u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e7t\|4e7t]], [[4e7u\|4e7u]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [http://www.ebi.ac.uk/pdbsum/4e7v PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [http://pdbe.org/4e7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [http://www.ebi.ac.uk/pdbsum/4e7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e7v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4e7v" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 17:54, 24 December 2014

2014-12-24T17:54:12Z

← Older revision		Revision as of 17:54, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E7V FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e7t\|4e7t]], [[4e7u\|4e7u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e7t\|4e7t]], [[4e7u\|4e7u]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [http://www.ebi.ac.uk/pdbsum/4e7v PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [http://www.ebi.ac.uk/pdbsum/4e7v PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Frankaer, C G.]]		[[Category: Frankaer, C G]]
	[[Category: Harris, P.]]		[[Category: Harris, P]]
	[[Category: Knudsen, M V.]]		[[Category: Knudsen, M V]]
	[[Category: Hormone]]		[[Category: Hormone]]
	[[Category: Zinc binding]]		[[Category: Zinc binding]]

OCA at 05:22, 24 September 2014

2014-09-24T05:22:00Z

← Older revision		Revision as of 05:22, 24 September 2014
Line 1:		Line 1:
	[[~~Image~~:~~4e7v~~.~~png~~\|~~left~~\|~~200px~~]]		==The structure of R6 bovine insulin==
			<StructureSection load='4e7v' size='340' side='right' caption='[[4e7v]], [[Resolution\|resolution]] 1.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4e7v]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E7V FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e7t\|4e7t]], [[4e7u\|4e7u]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e7v RCSB], [http://www.ebi.ac.uk/pdbsum/4e7v PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The crystal structures of three conformations, T(6), T(3)R(3) and R(6), of bovine insulin were solved at 1.40, 1.30 and 1.80 A resolution, respectively. All conformations crystallized in space group R3. In contrast to the T(6) and T(3)R(3) structures, different conformations of the N-terminal B-chain residue PheB1 were observed in the R(6) insulin structure, resulting in an eightfold doubling of the unit-cell volume upon cooling. The zinc coordination in each conformation was studied by X-ray absorption spectroscopy (XAS), including both EXAFS and XANES. Zinc adopts a tetrahedral coordination in all R(3) sites and an octahedral coordination in T(3) sites. The coordination distances were refined from XAS with a standard deviation of <0.01 A. In contrast to the distances determined from the medium-resolution crystal structures, the XAS results were in good agreement with similar coordination geometries found in small molecules, as well as in other high-resolution insulin structures. As the radiation dose for XRD experiments is two orders of magnitude higher compared with that of XAS experiments, the single crystals were exposed to a higher degree of radiation damage that affected the zinc coordination in the T(3) sites in particular. Furthermore, XANES spectra for the zinc sites in T(6) and R(6) insulin were successfully calculated using finite difference methods and the bond distances and angles were optimized from a quantitative XANES analysis.

	~~{{STRUCTURE_4e7v\| PDB=4e7v \| SCENE= }}~~		The structures of T(6), T(3)R(3) and R(6) bovine insulin: combining X-ray diffraction and absorption spectroscopy.,Frankaer CG, Knudsen MV, Noren K, Nazarenko E, Stahl K, Harris P Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1259-71. Epub 2012 Sep, 13. PMID:22993080<ref>PMID:22993080</ref>

	~~===The structure~~ of ~~R6 bovine insulin===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22993080}}~~

	~~==About this Structure==~~
	~~[[4e7v]] is a 32 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=4E7V OCA].~~

	==See Also==		==See Also==
	*[[Molecular Playground/Insulin\|Molecular Playground/Insulin]]		*[[Molecular Playground/Insulin\|Molecular Playground/Insulin]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Frankaer, C G.]]		[[Category: Frankaer, C G.]]

OCA at 07:42, 20 October 2012

2012-10-20T07:42:52Z

← Older revision		Revision as of 07:42, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[4e7v]] is a 32 chain structure ~~of [[Molecular Playground/Insulin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA].		[[4e7v]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA].

	==See Also==		==See Also==

OCA at 06:06, 3 October 2012

2012-10-03T06:06:38Z

← Older revision		Revision as of 06:06, 3 October 2012
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	===The structure of R6 bovine insulin===		===The structure of R6 bovine insulin===

			{{ABSTRACT_PUBMED_22993080}}

	==About this Structure==		==About this Structure==

OCA at 17:13, 25 July 2012

2012-07-25T17:13:01Z

← Older revision		Revision as of 17:13, 25 July 2012
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	[[Image:4e7v.~~jpg~~\|left\|200px]]		[[Image:4e7v.png\|left\|200px]]

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	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
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	{{STRUCTURE_4e7v\| PDB=4e7v \| SCENE= }}		{{STRUCTURE_4e7v\| PDB=4e7v \| SCENE= }}

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	==About this Structure==		==About this Structure==
	[[4e7v]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA].		[[4e7v]] is a 32 chain structure of [[Molecular Playground/Insulin]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E7V OCA].

			==See Also==
			*[[Molecular Playground/Insulin\|Molecular Playground/Insulin]]
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Frankaer, C G.]]		[[Category: Frankaer, C G.]]