4e8d - Revision history

OCA at 13:46, 8 November 2023

2023-11-08T13:46:25Z

← Older revision		Revision as of 13:46, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e8d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E8D FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e8d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E8D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8d OCA], [https://pdbe.org/4e8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e8d RCSB], [https://www.ebi.ac.uk/pdbsum/4e8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e8d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8d OCA], [https://pdbe.org/4e8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e8d RCSB], [https://www.ebi.ac.uk/pdbsum/4e8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e8d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/A0A0H2UN19_STRPN A0A0H2UN19_STRPN]]		[https://www.uniprot.org/uniprot/A0A0H2UN19_STRPN A0A0H2UN19_STRPN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4e8d" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4e8d" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Galactosidase 3D structures\|Galactosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:57, 28 September 2022

2022-09-28T06:57:03Z

← Older revision		Revision as of 06:57, 28 September 2022
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	==Crystal structure of streptococcal beta-galactosidase==		==Crystal structure of streptococcal beta-galactosidase==
	<StructureSection load='4e8d' size='340' side='right' caption='[[4e8d]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4e8d' size='340' side='right'caption='[[4e8d]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e8d]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strpn Strpn~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4E8D FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e8d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E8D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e8c\|4e8c]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8d OCA], [https://pdbe.org/4e8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e8d RCSB], [https://www.ebi.ac.uk/pdbsum/4e8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e8d ProSAT]</span></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SP_0060 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=170187 STRPN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4e8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8d OCA], [~~http~~://pdbe.org/4e8d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4e8d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4e8d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4e8d ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/A0A0H2UN19_STRPN A0A0H2UN19_STRPN]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Strpn~~]]		[[Category: Large Structures]]
	[[Category: Bai~~, X H~~]]		[[Category: Streptococcus pneumoniae TIGR4]]
	[[Category: Chen~~, Y X~~]]		[[Category: Bai XH]]
	[[Category: Cheng, W]]		[[Category: Chen YX]]
	[[Category: Jiang~~, Y L~~]]		[[Category: Cheng W]]
	[[Category: Li, Q]]		[[Category: Jiang YL]]
	[[Category: Wang, L]]		[[Category: Li Q]]
	[[Category: Yu, G]]		[[Category: Wang L]]
	[[Category: Zhou~~, C Z]]~~		[[Category: Yu G]]
	~~[[Category: Beta-propeller]]~~		[[Category: Zhou CZ]]
	~~[[Category: Glycohydrolase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Tim barrel~~]]

OCA at 19:24, 5 August 2016

2016-08-05T19:24:59Z

← Older revision		Revision as of 19:24, 5 August 2016
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	~~{{STRUCTURE_4e8d\| PDB=4e8d \| SCENE= }}~~
	~~===Crystal structure of streptococcal beta-galactosidase===~~
	~~{{ABSTRACT_PUBMED_22593580}}~~

	==~~About this Structure~~==		==Crystal structure of streptococcal beta-galactosidase==
	[[4e8d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_pneumoniae_tigr4 Streptococcus pneumoniae tigr4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA].		<StructureSection load='4e8d' size='340' side='right' caption='[[4e8d]], [[Resolution\|resolution]] 1.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4e8d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E8D FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e8c\|4e8c]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SP_0060 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=170187 STRPN])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8d OCA], [http://pdbe.org/4e8d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e8d RCSB], [http://www.ebi.ac.uk/pdbsum/4e8d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e8d ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The surface-exposed beta-galactosidase BgaC from Streptococcus pneumoniae was reported to be a virulence factor because of its specific hydrolysis activity towards the beta(1,3)-linked galactose and N-acetylglucosamine [Galbeta(1,3)NAG] moiety of oligosaccharides on the host molecules. Here we report the crystal structure of BgaC at 1.8 and its complex with galactose at 1.95 . At pH 5.5 to 8.0, BgaC exists as a stable homodimer, each subunit of which consists of three distinct domains: a catalytic domain of a classic (beta/alpha)8 TIM barrel, followed by two all-beta domains (ABDs) of unknown function. The side-walls of the TIM beta-barrel and a loop extended from the first ABD constitute the active site. Superposition of the galactose-complexed structure to the apo-form revealed significant conformational changes of residues Trp243 and Tyr455. Simulation of a putative substrate entrance tunnel and modeling of a complex structure with Galbeta(1,3)NAG enabled us to assign three key residues to the specific catalysis. Site-directed mutagenesis in combination with activity assays further proved that residues Trp240 and Tyr455 contribute to stabilizing the N-acetylglucosamine moiety, whereas Trp243 is critical for fixing the galactose ring. Moreover, we propose that BgaC and other galactosidases in the GH-35 family share a common domain organization and a conserved substrate-determinant aromatic residue protruding from the second domain.

	~~==Reference==~~		Structural insights into the substrate specificity of Streptococcus pneumoniae beta(1,3) galactosidase BgaC.,Cheng W, Wang L, Jiang YL, Bai XH, Chu J, Li Q, Yu G, Liang QL, Zhou CZ, Chen Y J Biol Chem. 2012 May 16. PMID:22593580<ref>PMID:22593580</ref>
	<ref ~~group="xtra"~~>PMID:~~022593580~~</ref><~~references group~~="~~xtra~~"/><references/>
	[[Category: ~~Streptococcus pneumoniae tigr4~~]]		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: Bai, X H.]]		</div>
	[[Category: Chen, Y X.]]		<div class="pdbe-citations 4e8d" style="background-color:#fffaf0;"></div>
	[[Category: Cheng, W.]]		== References ==
	[[Category: Jiang, Y L.]]		<references/>
	[[Category: Li, Q.]]		__TOC__
	[[Category: Wang, L.]]		</StructureSection>
	[[Category: Yu, G.]]		[[Category: Strpn]]
	[[Category: Zhou, C Z.]]		[[Category: Bai, X H]]
			[[Category: Chen, Y X]]
			[[Category: Cheng, W]]
			[[Category: Jiang, Y L]]
			[[Category: Li, Q]]
			[[Category: Wang, L]]
			[[Category: Yu, G]]
			[[Category: Zhou, C Z]]
	[[Category: Beta-propeller]]		[[Category: Beta-propeller]]
	[[Category: Glycohydrolase]]		[[Category: Glycohydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 19:26, 7 August 2013

2013-08-07T19:26:29Z

← Older revision		Revision as of 19:26, 7 August 2013
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	~~[[Image:4e8d.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_4e8d", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
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	{{STRUCTURE_4e8d\| PDB=4e8d \| SCENE= }}		{{STRUCTURE_4e8d\| PDB=4e8d \| SCENE= }}

	===Crystal structure of streptococcal beta-galactosidase===		===Crystal structure of streptococcal beta-galactosidase===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22593580}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 22593580 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_22593580}}		{{ABSTRACT_PUBMED_22593580}}

	==About this Structure==		==About this Structure==
	[[4e8d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_pneumoniae~~ Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA].		[[4e8d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae_tigr4 Streptococcus pneumoniae tigr4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:022593580</ref><references group="xtra"/>		<ref group="xtra">PMID:022593580</ref><references group="xtra"/><references/>
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae tigr4]]
	[[Category: Bai, X H.]]		[[Category: Bai, X H.]]
	[[Category: Chen, Y X.]]		[[Category: Chen, Y X.]]

OCA at 05:21, 13 June 2012

2012-06-13T05:21:53Z

← Older revision		Revision as of 05:21, 13 June 2012
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	[[Image:4e8d.~~jpg~~\|left\|200px]]		[[Image:4e8d.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of streptococcal beta-galactosidase===		===Crystal structure of streptococcal beta-galactosidase===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22593580}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22593580 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22593580}}

	==About this Structure==		==About this Structure==
	[[4e8d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA].		[[4e8d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8D OCA].

			==Reference==
			<ref group="xtra">PMID:022593580</ref><references group="xtra"/>
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
	[[Category: Bai, X H.]]		[[Category: Bai, X H.]]

OCA at 06:05, 30 May 2012

2012-05-30T06:05:28Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4e8d.jpg|left|200px]]
-The entry 4e8d is ON HOLD  until Paper Publication
+<!--
-Authors: Cheng, W., Wang, L., Bai, X.H., Jiang, Y.L., Li, Q., Yu, G., Zhou, C.Z., Chen, Y.X.
+===Crystal structure of streptococcal beta-galactosidase===
-Description: Crystal structure of streptococcal beta-galactosidase

OCA at 06:39, 11 April 2012

2012-04-11T06:39:30Z

← Older revision		Revision as of 06:39, 11 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4e8d is ON HOLD		The entry 4e8d is ON HOLD until Paper Publication

	Authors: Cheng, W., Wang, L., Bai, X.H., Jiang, Y.L., Li, Q., Yu, G., Zhou, C.Z., Chen, Y.X.		Authors: Cheng, W., Wang, L., Bai, X.H., Jiang, Y.L., Li, Q., Yu, G., Zhou, C.Z., Chen, Y.X.

	Description: Crystal structure of beta-galactosidase ~~from streptococcus pneumoniae~~		Description: Crystal structure of streptococcal beta-galactosidase

OCA at 05:46, 4 April 2012

2012-04-04T05:46:03Z

← Older revision		Revision as of 05:46, 4 April 2012
Line 3:		Line 3:
	The entry 4e8d is ON HOLD		The entry 4e8d is ON HOLD

	Authors: Cheng, W., Wang, L., Bai. X.H., Jiang, Y.L. , Li, Q., Yu, G., Zhou, C.Z., Chen, Y.X.		Authors: Cheng, W., Wang, L., Bai, X.H., Jiang, Y.L., Li, Q., Yu, G., Zhou, C.Z., Chen, Y.X.

	Description: Crystal structure of beta-galactosidase from streptococcus pneumoniae		Description: Crystal structure of beta-galactosidase from streptococcus pneumoniae

OCA: Protected "4e8d" [edit=sysop:move=sysop]

2012-03-28T07:24:22Z

Protected "4e8d" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:24, 28 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e8d is ON HOLD Authors: Cheng, W., Wang, L., Bai. X.H., Jiang, Y.L. , Li, Q., Yu, G., Zhou, C.Z., Chen, Y.X. Description: Crystal structure of bet...

2012-03-28T07:24:21Z

New page: '''Unreleased structure''' The entry 4e8d is ON HOLD Authors: Cheng, W., Wang, L., Bai. X.H., Jiang, Y.L. , Li, Q., Yu, G., Zhou, C.Z., Chen, Y.X. Description: Crystal structure of bet...

New page

'''Unreleased structure'''

The entry 4e8d is ON HOLD

Authors: Cheng, W., Wang, L., Bai. X.H., Jiang, Y.L. , Li, Q., Yu, G., Zhou, C.Z., Chen, Y.X.

Description: Crystal structure of beta-galactosidase from streptococcus pneumoniae