4e8o - Revision history

OCA at 19:35, 9 December 2016

2016-12-09T19:35:55Z

← Older revision		Revision as of 19:35, 9 December 2016
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	<StructureSection load='4e8o' size='340' side='right' caption='[[4e8o]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='4e8o' size='340' side='right' caption='[[4e8o]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e8o]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Aciba Aciba]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E8O FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e8o]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E8O FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac(6')-Ih ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 ACIBA])</td></tr>~~
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8o OCA], [http://pdbe.org/4e8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e8o RCSB], [http://www.ebi.ac.uk/pdbsum/4e8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e8o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8o OCA], [http://pdbe.org/4e8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e8o RCSB], [http://www.ebi.ac.uk/pdbsum/4e8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e8o ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Modification of aminoglycosides by N-acetyltransferases (AACs) is one of the major mechanisms of resistance to these antibiotics in human bacterial pathogens. More than fifty enzymes belonging to the AAC(6') subfamily have been identified in Gram-negative and Gram-positive clinical isolates. Our understanding of the molecular function and evolutionary origin of these resistance enzymes remains incomplete. Here we report the structural and enzymatic characterization of AAC(6')-Ig and AAC(6')-Ih from Acinetobacter spp. The crystal structure of AAC(6')-Ig in complex with tobramycin revealed a large substrate-binding cleft remaining partially unoccupied by the substrate, which is in stark contrast with the previously characterized AAC(6')-Ib enzyme. Enzymatic analysis indicated that AAC(6')-Ig and -Ih possess a broad specificity against aminoglycosides but with significantly lower turnover rates as compared to other AAC(6') enzymes. Structure- and function-informed phylogenetic analysis of AAC(6') enzymes led to identification of at least three distinct subfamilies varying in oligomeric state, active site composition and drug recognition mode. Our data support the concept of AAC(6') functionality originating through convergent evolution from diverse Gcn5-related-N-acetyltransferase (GNAT) ancestral enzymes, with AAC(6')-Ig/Ih representing enzymes that may still retain ancestral non-resistance functions in the cell as provided by their particular active site properties.

			Structural and biochemical characterization of Acinetobacter spp. aminoglycoside acetyltransferases highlights functional and evolutionary variation among antibiotic resistance enzymes.,Stogios PJ, Kuhn ML, Evdokimova E, Law M, Courvalin P, Savchenko A ACS Infect Dis. 2016 Oct 27. PMID:27785912<ref>PMID:27785912</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4e8o" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Aciba]]~~
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 11:46, 11 August 2016

2016-08-11T11:46:29Z

← Older revision		Revision as of 11:46, 11 August 2016
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	==Crystal structure of aminoglycoside antibiotic 6'-N-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii==		==Crystal structure of aminoglycoside antibiotic 6'-N-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii==
	<StructureSection load='4e8o' size='340' side='right' caption='[[4e8o]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='4e8o' size='340' side='right' caption='[[4e8o]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e8o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Acinetobacter_baumannii Acinetobacter baumannii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E8O FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e8o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aciba Aciba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E8O FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac(6')-Ih ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 ~~Acinetobacter baumannii~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac(6')-Ih ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 ACIBA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e8o RCSB], [http://www.ebi.ac.uk/pdbsum/4e8o PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8o OCA], [http://pdbe.org/4e8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e8o RCSB], [http://www.ebi.ac.uk/pdbsum/4e8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e8o ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Acinetobacter baumannii~~]]		[[Category: Aciba]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 13:54, 4 January 2015

2015-01-04T13:54:33Z

← Older revision		Revision as of 13:54, 4 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4e8o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E8O FirstGlance]. <br>		<table><tr><td colspan='2'>[[4e8o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E8O FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac(6')-Ih ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 Acinetobacter baumannii])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac(6')-Ih ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 Acinetobacter baumannii])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e8o RCSB], [http://www.ebi.ac.uk/pdbsum/4e8o PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e8o RCSB], [http://www.ebi.ac.uk/pdbsum/4e8o PDBsum]</span></td></tr>
	<table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Acinetobacter baumannii]]		[[Category: Acinetobacter baumannii]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F]]
	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]		[[Category: Structural genomic]]
	[[Category: Courvalin, P.]]		[[Category: Courvalin, P]]
	[[Category: Dong, A.]]		[[Category: Dong, A]]
	[[Category: Evdokimova, E.]]		[[Category: Evdokimova, E]]
	[[Category: Minasov, G.]]		[[Category: Minasov, G]]
	[[Category: Savchenko, A.]]		[[Category: Savchenko, A]]
	[[Category: Stogios, P J.]]		[[Category: Stogios, P J]]
	[[Category: Yim, V.]]		[[Category: Yim, V]]
	[[Category: Acetyl coenzyme some]]		[[Category: Acetyl coenzyme some]]
	[[Category: Aminoglycoside antibiotic 6'-n-acetyltransferase]]		[[Category: Aminoglycoside antibiotic 6'-n-acetyltransferase]]

OCA at 07:36, 5 June 2014

2014-06-05T07:36:53Z

← Older revision		Revision as of 07:36, 5 June 2014
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	~~[[Image:4e8o.png\|left\|200px]]~~		==Crystal structure of aminoglycoside antibiotic 6'-N-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii==
			<StructureSection load='4e8o' size='340' side='right' caption='[[4e8o]], [[Resolution\|resolution]] 2.14Å' scene=''>
	~~<!--~~		== Structural highlights ==
	~~The line below this paragraph, containing "STRUCTURE_4e8o", creates the "Structure Box" on the page.~~		<table><tr><td colspan='2'>[[4e8o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E8O FirstGlance]. <br>
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~		<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac(6')-Ih ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 Acinetobacter baumannii])</td></tr>
	~~or leave the SCENE parameter empty for the default display.~~		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e8o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e8o RCSB], [http://www.ebi.ac.uk/pdbsum/4e8o PDBsum]</span></td></tr>
	~~-->~~		<table>
	~~{{STRUCTURE_4e8o\| PDB=4e8o \| SCENE= }}~~		__TOC__
			</StructureSection>
	===Crystal structure of aminoglycoside antibiotic 6'-N-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii===


	==~~About this Structure~~==
	[[4e8o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E8O OCA].
	[[Category: Acinetobacter baumannii]]		[[Category: Acinetobacter baumannii]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]

OCA at 06:09, 11 April 2012

2012-04-11T06:09:17Z

← Older revision		Revision as of 06:09, 11 April 2012
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	[[Image:4e8o.~~jpg~~\|left\|200px]]		[[Image:4e8o.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_4e8o\| PDB=4e8o \| SCENE= }}		{{STRUCTURE_4e8o\| PDB=4e8o \| SCENE= }}

	===Crystal structure of aminoglycoside antibiotic 6'-O-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii===		===Crystal structure of aminoglycoside antibiotic 6'-N-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii===


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	[[Category: Yim, V.]]		[[Category: Yim, V.]]
	[[Category: Acetyl coenzyme some]]		[[Category: Acetyl coenzyme some]]
	[[Category: Aminoglycoside antibiotic 6'-o-acetyltransferase]]		[[Category: Aminoglycoside antibiotic 6'-n-acetyltransferase]]
	[[Category: Aminoglycoside antibiotic]]		[[Category: Aminoglycoside antibiotic]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]

OCA at 06:20, 4 April 2012

2012-04-04T06:20:58Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4e8o.jpg|left|200px]]
-The entry 4e8o is ON HOLD
+<!--
-Authors: Stogios, P.J., Minasov, G., Dong, A., Evdokimova, E., Yim, V., Courvalin, P., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)
+===Crystal structure of aminoglycoside antibiotic 6'-O-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii===
-Description: Crystal structure of aminoglycoside antibiotic 6'-O-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii

OCA: Protected "4e8o" [edit=sysop:move=sysop]

2012-03-28T07:24:43Z

Protected "4e8o" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:24, 28 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4e8o is ON HOLD Authors: Stogios, P.J., Minasov, G., Dong, A., Evdokimova, E., Yim, V., Courvalin, P., Savchenko, A., Anderson, W.F., Center for Str...

2012-03-28T07:24:42Z

New page: '''Unreleased structure''' The entry 4e8o is ON HOLD Authors: Stogios, P.J., Minasov, G., Dong, A., Evdokimova, E., Yim, V., Courvalin, P., Savchenko, A., Anderson, W.F., Center for Str...

New page

'''Unreleased structure'''

The entry 4e8o is ON HOLD

Authors: Stogios, P.J., Minasov, G., Dong, A., Evdokimova, E., Yim, V., Courvalin, P., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: Crystal structure of aminoglycoside antibiotic 6'-O-acetyltransferase AAC(6')-Ih from Acinetobacter baumannii