4eam - Revision history

OCA at 14:56, 14 March 2024

2024-03-14T14:56:49Z

← Older revision		Revision as of 14:56, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eam]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EAM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eam]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EAM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eam OCA], [https://pdbe.org/4eam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eam RCSB], [https://www.ebi.ac.uk/pdbsum/4eam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eam ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eam OCA], [https://pdbe.org/4eam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eam RCSB], [https://www.ebi.ac.uk/pdbsum/4eam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eam ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/BGAL_SACS2 BGAL_SACS2]]		[https://www.uniprot.org/uniprot/BGAL_SACS2 BGAL_SACS2]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Ligand-dependent activity has been engineered into enzymes for purposes ranging from controlling cell morphology to reprogramming cellular signaling pathways. Where these successes have typically fused a naturally allosteric domain to the enzyme of interest, here we instead demonstrate an approach for designing a de novo allosteric effector site directly into the catalytic domain of an enzyme. This approach is distinct from traditional chemical rescue of enzymes in that it relies on disruption and restoration of structure, rather than active site chemistry, as a means to achieve modulate function. We present two examples, W33G in a beta-glycosidase enzyme (beta-gly) and W492G in a beta-glucuronidase enzyme (beta-gluc), in which we engineer indole-dependent activity into enzymes by removing a buried tryptophan side chain that serves as a buttress for the active site architecture. In both cases, we observe a loss of function, and in both cases we find that the subsequent addition of indole can be used to restore activity. Through a detailed analysis of beta-gly W33G kinetics, we demonstrate that this rescued enzyme is fully functionally equivalent to the corresponding wild-type enzyme. We then present the apo and indole-bound crystal structures of beta-gly W33G, which together establish the structural basis for enzyme inactivation and rescue. Finally, we use this designed switch to modulate beta-glycosidase activity in living cells using indole. Disruption and recovery of protein structure may represent a general technique for introducing allosteric control into enzymes, and thus may serve as a starting point for building a variety of bioswitches and sensors.

	Designing Allosteric Control into Enzymes by Chemical Rescue of Structure.,Deckert K, Budiardjo SJ, Brunner LC, Lovell S, Karanicolas J J Am Chem Soc. 2012 Jun 11. PMID:22655749<ref>PMID:22655749</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4eam" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Galactosidase 3D structures\|Galactosidase 3D structures]]		*[[Galactosidase 3D structures\|Galactosidase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:00, 28 September 2022

2022-09-28T07:00:54Z

← Older revision		Revision as of 07:00, 28 September 2022
Line 1:		Line 1:

	==1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus==		==1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus==
	<StructureSection load='4eam' size='340' side='right' caption='[[4eam]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4eam' size='340' side='right'caption='[[4eam]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eam]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Sulso Sulso~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EAM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EAM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eam]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EAM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ean\|4ean]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eam OCA], [https://pdbe.org/4eam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eam RCSB], [https://www.ebi.ac.uk/pdbsum/4eam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eam ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lacS, SSO3019 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eam OCA], [~~http~~://pdbe.org/4eam PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eam RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eam PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eam ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/BGAL_SACS2 BGAL_SACS2]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4eam" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4eam" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Galactosidase 3D structures\|Galactosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Beta-galactosidase~~]]		[[Category: Large Structures]]
	[[Category: ~~Sulso~~]]		[[Category: Saccharolobus solfataricus P2]]
	[[Category: Battaile~~, K P~~]]		[[Category: Battaile KP]]
	[[Category: Brunner~~, L C~~]]		[[Category: Brunner LC]]
	[[Category: Budiardjo~~, S J~~]]		[[Category: Budiardjo SJ]]
	[[Category: Deckert, K]]		[[Category: Deckert K]]
	[[Category: Karanicolas, J]]		[[Category: Karanicolas J]]
	[[Category: Lovell, S]]		[[Category: Lovell S]]
	~~[[Category: Allosteric activation]]~~
	~~[[Category: Chemical biology]]~~
	~~[[Category: Chemical rescue]]~~
	~~[[Category: Glycoside hydrolase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Switchable enzyme~~]]

OCA at 08:00, 15 November 2017

2017-11-15T08:00:55Z

← Older revision		Revision as of 08:00, 15 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4eam" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4eam" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Galactosidase\|Galactosidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:36, 11 December 2016

2016-12-11T02:36:02Z

← Older revision		Revision as of 02:36, 11 December 2016
Line 1:		Line 1:

	==1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus==		==1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus==
	<StructureSection load='4eam' size='340' side='right' caption='[[4eam]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4eam' size='340' side='right' caption='[[4eam]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eam]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EAM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eam]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EAM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ean\|4ean]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ean\|4ean]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lacS, SSO3019 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 ~~Sulfolobus solfataricus P2~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lacS, SSO3019 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eam OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eam RCSB], [http://www.ebi.ac.uk/pdbsum/4eam PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eam OCA], [http://pdbe.org/4eam PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eam RCSB], [http://www.ebi.ac.uk/pdbsum/4eam PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eam ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eam" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Beta-galactosidase]]		[[Category: Beta-galactosidase]]
	[[Category: ~~Sulfolobus solfataricus p2~~]]		[[Category: Sulso]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Brunner, L C]]		[[Category: Brunner, L C]]

OCA at 17:08, 9 December 2014

2014-12-09T17:08:57Z

← Older revision		Revision as of 17:08, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~4eam~~.~~png~~\|~~left~~\|~~200px~~]]		==1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus==
			<StructureSection load='4eam' size='340' side='right' caption='[[4eam]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4eam]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EAM FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ean\|4ean]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lacS, SSO3019 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 Sulfolobus solfataricus P2])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eam OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eam RCSB], [http://www.ebi.ac.uk/pdbsum/4eam PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Ligand-dependent activity has been engineered into enzymes for purposes ranging from controlling cell morphology to reprogramming cellular signaling pathways. Where these successes have typically fused a naturally allosteric domain to the enzyme of interest, here we instead demonstrate an approach for designing a de novo allosteric effector site directly into the catalytic domain of an enzyme. This approach is distinct from traditional chemical rescue of enzymes in that it relies on disruption and restoration of structure, rather than active site chemistry, as a means to achieve modulate function. We present two examples, W33G in a beta-glycosidase enzyme (beta-gly) and W492G in a beta-glucuronidase enzyme (beta-gluc), in which we engineer indole-dependent activity into enzymes by removing a buried tryptophan side chain that serves as a buttress for the active site architecture. In both cases, we observe a loss of function, and in both cases we find that the subsequent addition of indole can be used to restore activity. Through a detailed analysis of beta-gly W33G kinetics, we demonstrate that this rescued enzyme is fully functionally equivalent to the corresponding wild-type enzyme. We then present the apo and indole-bound crystal structures of beta-gly W33G, which together establish the structural basis for enzyme inactivation and rescue. Finally, we use this designed switch to modulate beta-glycosidase activity in living cells using indole. Disruption and recovery of protein structure may represent a general technique for introducing allosteric control into enzymes, and thus may serve as a starting point for building a variety of bioswitches and sensors.

	~~{{STRUCTURE_4eam\| PDB=4eam \| SCENE= }}~~		Designing Allosteric Control into Enzymes by Chemical Rescue of Structure.,Deckert K, Budiardjo SJ, Brunner LC, Lovell S, Karanicolas J J Am Chem Soc. 2012 Jun 11. PMID:22655749<ref>PMID:22655749</ref>

	~~===1~~.~~70A resolution structure~~ of ~~apo beta-glycosidase (W33G) from sulfolobus solfataricus===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22655749}}~~		==See Also==
			*[[Galactosidase\|Galactosidase]]
	==~~About this Structure~~==		== References ==
	[[~~4eam]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EAM OCA~~].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:022655749<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Beta-galactosidase]]		[[Category: Beta-galactosidase]]
	[[Category: Sulfolobus solfataricus p2]]		[[Category: Sulfolobus solfataricus p2]]
	[[Category: Battaile, K P.]]		[[Category: Battaile, K P]]
	[[Category: Brunner, L C.]]		[[Category: Brunner, L C]]
	[[Category: Budiardjo, S J.]]		[[Category: Budiardjo, S J]]
	[[Category: Deckert, K.]]		[[Category: Deckert, K]]
	[[Category: Karanicolas, J.]]		[[Category: Karanicolas, J]]
	[[Category: Lovell, S.]]		[[Category: Lovell, S]]
	[[Category: Allosteric activation]]		[[Category: Allosteric activation]]
	[[Category: Chemical biology]]		[[Category: Chemical biology]]

OCA at 09:55, 21 November 2012

2012-11-21T09:55:35Z

← Older revision		Revision as of 09:55, 21 November 2012
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	[[Image:4eam.~~jpg~~\|left\|200px]]		[[Image:4eam.png\|left\|200px]]

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	{{STRUCTURE_4eam\| PDB=4eam \| SCENE= }}		{{STRUCTURE_4eam\| PDB=4eam \| SCENE= }}

	===1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus===		===1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus===


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	~~(as it appears on PubMed at http://www.pubmed.gov), where 22655749 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_22655749}}		{{ABSTRACT_PUBMED_22655749}}

OCA at 06:06, 13 June 2012

2012-06-13T06:06:52Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4eam.jpg|left|200px]]
-The entry 4eam is ON HOLD  until Paper Publication
+<!--
-Authors: Lovell, S., Battaile, K.P., Deckert, K., Brunner, L.C., Budiardjo, S.J., Karanicolas, J.
+===1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus===
-Description: 1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus

OCA at 05:46, 4 April 2012

2012-04-04T05:46:49Z

← Older revision		Revision as of 05:46, 4 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eam is ON HOLD		The entry 4eam is ON HOLD until Paper Publication

	Authors: Lovell, ~~Scott~~, Battaile, ~~Kevin~~ P., Deckert, ~~Katelyn~~, Brunner, ~~Luke~~ C., Budiardjo, S. ~~Jimmy~~, Karanicolas, ~~John~~.		Authors: Lovell, S., Battaile, K.P., Deckert, K., Brunner, L.C., Budiardjo, S.J., Karanicolas, J.

	Description: 1.70A ~~RESOLUTION STRUCTURE OF APO BETA~~-~~GLYCOSIDASE~~ (W33G) ~~FROM SULFOLOBUS SOLFATARICUS~~		Description: 1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus

OCA: Protected "4eam" [edit=sysop:move=sysop]

2012-03-28T07:26:53Z

Protected "4eam" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:26, 28 March 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4eam is ON HOLD Authors: Lovell, Scott, Battaile, Kevin P., Deckert, Katelyn, Brunner, Luke C., Budiardjo, S. Jimmy, Karanicolas, John. Description...

2012-03-28T07:26:52Z

New page: '''Unreleased structure''' The entry 4eam is ON HOLD Authors: Lovell, Scott, Battaile, Kevin P., Deckert, Katelyn, Brunner, Luke C., Budiardjo, S. Jimmy, Karanicolas, John. Description...

New page

'''Unreleased structure'''

The entry 4eam is ON HOLD

Authors: Lovell, Scott, Battaile, Kevin P., Deckert, Katelyn, Brunner, Luke C., Budiardjo, S. Jimmy, Karanicolas, John.

Description: 1.70A RESOLUTION STRUCTURE OF APO BETA-GLYCOSIDASE (W33G) FROM SULFOLOBUS SOLFATARICUS