4ec2 - Revision history

OCA at 14:57, 14 March 2024

2024-03-14T14:57:36Z

← Older revision		Revision as of 14:57, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ec2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ec2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EC2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.002Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [https://pdbe.org/4ec2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ec2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ec2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [https://pdbe.org/4ec2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ec2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ec2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/FRDA_YEAST FRDA_YEAST]] Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. Plays a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+). Can store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization. May be involved in regulation of the mitochondrial electron transport chain.<ref>PMID:9180083</ref> <ref>PMID:9988680</ref> <ref>PMID:15961414</ref> <ref>PMID:16371422</ref> <ref>PMID:19884169</ref> <ref>PMID:17027502</ref>		[https://www.uniprot.org/uniprot/FRDA_YEAST FRDA_YEAST] Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. Plays a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+). Can store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization. May be involved in regulation of the mitochondrial electron transport chain.<ref>PMID:9180083</ref> <ref>PMID:9988680</ref> <ref>PMID:15961414</ref> <ref>PMID:16371422</ref> <ref>PMID:19884169</ref> <ref>PMID:17027502</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The role of the mitochondrial protein frataxin in iron storage and detoxification, iron delivery to iron-sulfur cluster biosynthesis, heme biosynthesis, and aconitase repair has been extensively studied during the last decade. However, still no general consensus exists on the details of the mechanism of frataxin function and oligomerization. Here, using small-angle x-ray scattering and x-ray crystallography, we describe the solution structure of the oligomers formed during the iron-dependent assembly of yeast (Yfh1) and Escherichia coli (CyaY) frataxin. At an iron-to-protein ratio of 2, the initially monomeric Yfh1 is converted to a trimeric form in solution. The trimer in turn serves as the assembly unit for higher order oligomers induced at higher iron-to-protein ratios. The x-ray crystallographic structure obtained from iron-soaked crystals demonstrates that iron binds at the trimer-trimer interaction sites, presumably contributing to oligomer stabilization. For the ferroxidation-deficient D79A/D82A variant of Yfh1, iron-dependent oligomerization may still take place, although >50% of the protein is found in the monomeric state at the highest iron-to-protein ratio used. This demonstrates that the ferroxidation reaction controls frataxin assembly and presumably the iron chaperone function of frataxin and its interactions with target proteins. For E. coli CyaY, the assembly unit of higher order oligomers is a tetramer, which could be an effect of the much shorter N-terminal region of this protein. The results show that understanding of the mechanistic features of frataxin function requires detailed knowledge of the interplay between the ferroxidation reaction, iron-induced oligomerization, and the structure of oligomers formed during assembly.

	The molecular basis of iron-induced oligomerization of frataxin and the role of the ferroxidation reaction in oligomerization.,Soderberg CA, Rajan S, Shkumatov AV, Gakh O, Schaefer S, Ahlgren EC, Svergun DI, Isaya G, Al-Karadaghi S J Biol Chem. 2013 Mar 22;288(12):8156-67. doi: 10.1074/jbc.M112.442285. Epub 2013, Jan 23. PMID:23344952<ref>PMID:23344952</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4ec2" style="background-color:#fffaf0;"></div~~>

	==See Also==		==See Also==

OCA at 07:03, 28 September 2022

2022-09-28T07:03:58Z

← Older revision		Revision as of 07:03, 28 September 2022
Line 1:		Line 1:

	==Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous==		==Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous==
	<StructureSection load='4ec2' size='340' side='right' caption='[[4ec2]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4ec2' size='340' side='right'caption='[[4ec2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ec2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ec2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EC2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3oer\|3oer]], [[3oeq\|3oeq]], [[2fql\|2fql]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [https://pdbe.org/4ec2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ec2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ec2 ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YFH1, YDL120W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [~~http~~://pdbe.org/4ec2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ec2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ec2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/FRDA_YEAST FRDA_YEAST]] Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. Plays a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+). Can store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization. May be involved in regulation of the mitochondrial electron transport chain.<ref>PMID:9180083</ref> <ref>PMID:9988680</ref> <ref>PMID:15961414</ref> <ref>PMID:16371422</ref> <ref>PMID:19884169</ref> <ref>PMID:17027502</ref>		[[https://www.uniprot.org/uniprot/FRDA_YEAST FRDA_YEAST]] Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. Plays a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+). Can store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization. May be involved in regulation of the mitochondrial electron transport chain.<ref>PMID:9180083</ref> <ref>PMID:9988680</ref> <ref>PMID:15961414</ref> <ref>PMID:16371422</ref> <ref>PMID:19884169</ref> <ref>PMID:17027502</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4ec2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4ec2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Frataxin 3D Structures\|Frataxin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Baker's yeast~~]]		[[Category: Large Structures]]
	[[Category: ~~Ferroxidase~~]]		[[Category: Saccharomyces cerevisiae S288C]]
	[[Category: Al-Karadaghi, S]]		[[Category: Al-Karadaghi S]]
	[[Category: Gakh, O]]		[[Category: Gakh O]]
	[[Category: Isaya, G]]		[[Category: Isaya G]]
	[[Category: Rajan, S]]		[[Category: Rajan S]]
	[[Category: Soderberg~~, C A.G]]~~		[[Category: Soderberg CAG]]
	~~[[Category: Alpha/beta sandwich]]~~
	~~[[Category: Iron-storage]]~~
	~~[[Category: Metallochaperone]]~~
	~~[[Category: Transport protein~~]]

OCA at 09:55, 5 August 2016

2016-08-05T09:55:32Z

← Older revision		Revision as of 09:55, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous==		==Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous==
	<StructureSection load='4ec2' size='340' side='right' caption='[[4ec2]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4ec2' size='340' side='right' caption='[[4ec2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ec2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ec2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EC2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3oer\|3oer]], [[3oeq\|3oeq]], [[2fql\|2fql]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3oer\|3oer]], [[3oeq\|3oeq]], [[2fql\|2fql]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YFH1, YDL120W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YFH1, YDL120W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ec2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [http://pdbe.org/4ec2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ec2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ec2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4ec2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Baker's yeast]]
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
	~~[[Category: Saccharomyces cerevisiae s288c]]~~
	[[Category: Al-Karadaghi, S]]		[[Category: Al-Karadaghi, S]]
	[[Category: Gakh, O]]		[[Category: Gakh, O]]

OCA at 01:23, 25 December 2014

2014-12-25T01:23:01Z

← Older revision		Revision as of 01:23, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ec2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ec2 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/FRDA_YEAST FRDA_YEAST]] Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. Plays a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+). Can store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization. May be involved in regulation of the mitochondrial electron transport chain.<ref>PMID:9180083</ref> <ref>PMID:9988680</ref> <ref>PMID:15961414</ref> <ref>PMID:16371422</ref> <ref>PMID:19884169</ref> <ref>PMID:17027502</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:25, 21 December 2014

2014-12-21T11:25:29Z

← Older revision		Revision as of 11:25, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4ec2\| PDB=4ec2 \| SCENE= }}~~		==Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous==
	===Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous===		<StructureSection load='4ec2' size='340' side='right' caption='[[4ec2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	~~{{ABSTRACT_PUBMED_23344952}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4ec2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EC2 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3oer\|3oer]], [[3oeq\|3oeq]], [[2fql\|2fql]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YFH1, YDL120W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ec2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ec2 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The role of the mitochondrial protein frataxin in iron storage and detoxification, iron delivery to iron-sulfur cluster biosynthesis, heme biosynthesis, and aconitase repair has been extensively studied during the last decade. However, still no general consensus exists on the details of the mechanism of frataxin function and oligomerization. Here, using small-angle x-ray scattering and x-ray crystallography, we describe the solution structure of the oligomers formed during the iron-dependent assembly of yeast (Yfh1) and Escherichia coli (CyaY) frataxin. At an iron-to-protein ratio of 2, the initially monomeric Yfh1 is converted to a trimeric form in solution. The trimer in turn serves as the assembly unit for higher order oligomers induced at higher iron-to-protein ratios. The x-ray crystallographic structure obtained from iron-soaked crystals demonstrates that iron binds at the trimer-trimer interaction sites, presumably contributing to oligomer stabilization. For the ferroxidation-deficient D79A/D82A variant of Yfh1, iron-dependent oligomerization may still take place, although >50% of the protein is found in the monomeric state at the highest iron-to-protein ratio used. This demonstrates that the ferroxidation reaction controls frataxin assembly and presumably the iron chaperone function of frataxin and its interactions with target proteins. For E. coli CyaY, the assembly unit of higher order oligomers is a tetramer, which could be an effect of the much shorter N-terminal region of this protein. The results show that understanding of the mechanistic features of frataxin function requires detailed knowledge of the interplay between the ferroxidation reaction, iron-induced oligomerization, and the structure of oligomers formed during assembly.

	~~==Function==~~		The molecular basis of iron-induced oligomerization of frataxin and the role of the ferroxidation reaction in oligomerization.,Soderberg CA, Rajan S, Shkumatov AV, Gakh O, Schaefer S, Ahlgren EC, Svergun DI, Isaya G, Al-Karadaghi S J Biol Chem. 2013 Mar 22;288(12):8156-67. doi: 10.1074/jbc.M112.442285. Epub 2013, Jan 23. PMID:23344952<ref>PMID:23344952</ref>
	~~[[http://www.uniprot.org/uniprot/FRDA_YEAST FRDA_YEAST]] Promotes the biosynthesis of heme as well as the assembly and repair~~ of iron-~~sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. Plays a~~ role ~~in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+). Can store large amounts~~ of the ~~metal~~ in ~~the form of a ferrihydrite mineral by~~ oligomerization. ~~May be involved in regulation of the mitochondrial electron transport chain~~.~~<ref>PMID~~:~~9180083</ref> <ref>PMID~~:~~9988680<~~/~~ref> <ref>PMID:15961414</ref> <ref>~~PMID:~~16371422</ref> <ref>PMID:19884169</ref>~~ <ref>PMID:~~17027502~~</ref>

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[4ec2]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4EC2 OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:023344952</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
	[[Category: Al-Karadaghi, S.]]		[[Category: Al-Karadaghi, S]]
	[[Category: Gakh, O.]]		[[Category: Gakh, O]]
	[[Category: Isaya, G.]]		[[Category: Isaya, G]]
	[[Category: Rajan, S.]]		[[Category: Rajan, S]]
	[[Category: Soderberg, C A.G.]]		[[Category: Soderberg, C A.G]]
	[[Category: Alpha/beta sandwich]]		[[Category: Alpha/beta sandwich]]
	[[Category: Iron-storage]]		[[Category: Iron-storage]]
	[[Category: Metallochaperone]]		[[Category: Metallochaperone]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 15:51, 19 June 2013

2013-06-19T15:51:33Z

← Older revision		Revision as of 15:51, 19 June 2013
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	~~[[Image:4ec2.jpg\|left\|200px]]~~

	{{STRUCTURE_4ec2\| PDB=4ec2 \| SCENE= }}		{{STRUCTURE_4ec2\| PDB=4ec2 \| SCENE= }}

	===Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous===		===Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous===
			{{ABSTRACT_PUBMED_23344952}}

	~~{{ABSTRACT_PUBMED_22051511}}~~		==Function==
			[[http://www.uniprot.org/uniprot/FRDA_YEAST FRDA_YEAST]] Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. Plays a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+). Can store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization. May be involved in regulation of the mitochondrial electron transport chain.<ref>PMID:9180083</ref> <ref>PMID:9988680</ref> <ref>PMID:15961414</ref> <ref>PMID:16371422</ref> <ref>PMID:19884169</ref> <ref>PMID:17027502</ref>

	==About this Structure==		==About this Structure==
Line 11:		Line 10:

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~022051511~~</ref><~~ref~~ group="xtra"~~>PMID:017027502<~~/~~ref~~><references ~~group="xtra"~~/>		<ref group="xtra">PMID:023344952</ref><references group="xtra"/><references/>
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]

OCA at 12:38, 30 January 2013

2013-01-30T12:38:45Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4ec2.jpg|left|200px]]
-The entry 4ec2 is ON HOLD  until Paper Publication
+{{STRUCTURE_4ec2|  PDB=4ec2  |  SCENE=  }}
-Authors: Soderberg, C.A.G., Rajan, S., Gakh, O., Isaya, G., Al-Karadaghi, S.
+===Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous===
-Description: Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous
+{{ABSTRACT_PUBMED_22051511}}

OCA at 05:36, 23 May 2012

2012-05-23T05:36:19Z

← Older revision		Revision as of 05:36, 23 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4ec2 is ON HOLD		The entry 4ec2 is ON HOLD until Paper Publication

	Authors: Soderberg, C.A.G., Rajan, S., Gakh, O., Isaya, G., Al-Karadaghi, S.		Authors: Soderberg, C.A.G., Rajan, S., Gakh, O., Isaya, G., Al-Karadaghi, S.

	Description: Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous		Description: Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous

OCA at 08:51, 25 April 2012

2012-04-25T08:51:49Z

← Older revision		Revision as of 08:51, 25 April 2012
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	Authors: Soderberg, C.A.G., Rajan, S., Gakh, O., Isaya, G., Al-Karadaghi, S.		Authors: Soderberg, C.A.G., Rajan, S., Gakh, O., Isaya, G., Al-Karadaghi, S.

	Description: ~~CRYSTAL STRUCTURE OF TRIMERIC FRATAXIN FROM THE YEAST SACCHAROMYCES CEREVISIAE~~, ~~COMPLEXED WITH FERROUS~~		Description: Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous

OCA: Protected "4ec2" [edit=sysop:move=sysop]

2012-04-04T05:48:00Z

Protected "4ec2" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:48, 4 April 2012
(No difference)