4ee2 - Revision history

OCA at 14:58, 14 March 2024

2024-03-14T14:58:41Z

← Older revision		Revision as of 14:58, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ee2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EE2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ee2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EE2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.91Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [https://pdbe.org/4ee2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ee2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [https://pdbe.org/4ee2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ee2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN]] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.		[https://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Many toxins assemble into oligomers on the surface of cells. Local chemical cues signal and trigger critical rearrangements of the oligomer, inducing the formation of a membrane-fused or channel state. Bacillus anthracis secretes two virulence factors: a tripartite toxin and a poly-gamma-d-glutamic acid capsule (gamma-DPGA). The toxin's channel-forming component, protective antigen (PA), oligomerizes to create a prechannel that forms toxic complexes upon binding the two other enzyme components, lethal factor (LF) and edema factor (EF). Following endocytosis into host cells, acidic pH signals the prechannel to form the channel state, which translocates LF and EF into the host cytosol. We report gamma-DPGA binds to PA, LF, and EF, exhibiting nanomolar avidity for the PA prechannel oligomer. We show PA channel formation requires the pH-dependent disruption of the intra-PA domain-2-domain-4 (D2-D4) interface. gamma-DPGA stabilizes the D2-D4 interface, preventing channel formation both in model membranes and cultured mammalian cells. A 1.9-A resolution X-ray crystal structure of a D2-D4-interface mutant and corresponding functional studies reveal how stability at the intra-PA interface governs channel formation. We also pinpoint the kinetic pH trigger for channel formation to a residue within PA's membrane-insertion loop at the inter-PA D2-D4 interface. Thus, gamma-DPGA may function as a chemical cue, signaling that the local environment is appropriate for toxin assembly but inappropriate for channel formation.

	Anthrax toxin protective antigen integrates poly-gamma-D-glutamate and pH signals to sense the optimal environment for channel formation.,Kintzer AF, Tang II, Schawel AK, Brown MJ, Krantz BA Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18378-83. doi:, 10.1073/pnas.1208280109. Epub 2012 Oct 24. PMID:23100533<ref>PMID:23100533</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4ee2" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:07, 28 September 2022

2022-09-28T07:07:01Z

← Older revision		Revision as of 07:07, 28 September 2022
Line 1:		Line 1:

	==Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution==		==Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution==
	<StructureSection load='4ee2' size='340' side='right' caption='[[4ee2]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='4ee2' size='340' side='right'caption='[[4ee2]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ee2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var.~~ anthracis~~" (cohn 1872) smith et al. 1946~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EE2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ee2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EE2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acc\|1acc]], [[3tew\|3tew]], [[3tex\|3tex]], [[3hvd\|3hvd]], [[1tzo\|1tzo]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [https://pdbe.org/4ee2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ee2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee2 ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pagA, pag, pXO1-110, BXA0164, GBAA_pXO1_0164 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [~~http~~://pdbe.org/4ee2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ee2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN]] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.		[[https://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN]] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Kintzer, A F~~]]		[[Category: Bacillus anthracis]]
	[[Category: ~~Krantz, B A~~]]		[[Category: Large Structures]]
	[[Category: ~~Anthrax toxin~~]]		[[Category: Kintzer AF]]
	[[Category: ~~Assembly]]~~		[[Category: Krantz BA]]
	~~[[Category: Cell-binding]]~~
	~~[[Category: Channel formation]]~~
	~~[[Category: Protein translocation]]~~
	~~[[Category: Toxin]]~~
	~~[[Category: Transport protein~~]]

OCA at 08:02, 15 November 2017

2017-11-15T08:02:11Z

← Older revision		Revision as of 08:02, 15 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4ee2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4ee2" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Anthrax protective antigen\|Anthrax protective antigen]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:50, 4 August 2016

2016-08-04T11:50:52Z

← Older revision		Revision as of 11:50, 4 August 2016
Line 1:		Line 1:

	==Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution==		==Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution==
	<StructureSection load='4ee2' size='340' side='right' caption='[[4ee2]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='4ee2' size='340' side='right' caption='[[4ee2]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ee2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_anthracis Bacillus~~ anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EE2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ee2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EE2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acc\|1acc]], [[3tew\|3tew]], [[3tex\|3tex]], [[3hvd\|3hvd]], [[1tzo\|1tzo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acc\|1acc]], [[3tew\|3tew]], [[3tex\|3tex]], [[3hvd\|3hvd]], [[1tzo\|1tzo]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pagA, pag, pXO1-110, BXA0164, GBAA_pXO1_0164 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pagA, pag, pXO1-110, BXA0164, GBAA_pXO1_0164 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [http://pdbe.org/4ee2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4ee2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Bacillus anthracis]]~~
	[[Category: Kintzer, A F]]		[[Category: Kintzer, A F]]
	[[Category: Krantz, B A]]		[[Category: Krantz, B A]]

OCA at 14:09, 24 December 2014

2014-12-24T14:09:16Z

← Older revision		Revision as of 14:09, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee2 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN]] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:42, 21 December 2014

2014-12-21T11:42:40Z

← Older revision		Revision as of 11:42, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4ee2\| PDB=4ee2 \| SCENE= }}~~		==Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution==
	===Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution===		<StructureSection load='4ee2' size='340' side='right' caption='[[4ee2]], [[Resolution\|resolution]] 1.91Å' scene=''>
	~~{{ABSTRACT_PUBMED_23100533}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4ee2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EE2 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acc\|1acc]], [[3tew\|3tew]], [[3tex\|3tex]], [[3hvd\|3hvd]], [[1tzo\|1tzo]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pagA, pag, pXO1-110, BXA0164, GBAA_pXO1_0164 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ee2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee2 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Many toxins assemble into oligomers on the surface of cells. Local chemical cues signal and trigger critical rearrangements of the oligomer, inducing the formation of a membrane-fused or channel state. Bacillus anthracis secretes two virulence factors: a tripartite toxin and a poly-gamma-d-glutamic acid capsule (gamma-DPGA). The toxin's channel-forming component, protective antigen (PA), oligomerizes to create a prechannel that forms toxic complexes upon binding the two other enzyme components, lethal factor (LF) and edema factor (EF). Following endocytosis into host cells, acidic pH signals the prechannel to form the channel state, which translocates LF and EF into the host cytosol. We report gamma-DPGA binds to PA, LF, and EF, exhibiting nanomolar avidity for the PA prechannel oligomer. We show PA channel formation requires the pH-dependent disruption of the intra-PA domain-2-domain-4 (D2-D4) interface. gamma-DPGA stabilizes the D2-D4 interface, preventing channel formation both in model membranes and cultured mammalian cells. A 1.9-A resolution X-ray crystal structure of a D2-D4-interface mutant and corresponding functional studies reveal how stability at the intra-PA interface governs channel formation. We also pinpoint the kinetic pH trigger for channel formation to a residue within PA's membrane-insertion loop at the inter-PA D2-D4 interface. Thus, gamma-DPGA may function as a chemical cue, signaling that the local environment is appropriate for toxin assembly but inappropriate for channel formation.

	~~==Function==~~		Anthrax toxin protective antigen integrates poly-gamma-D-glutamate and pH signals to sense the optimal environment for channel formation.,Kintzer AF, Tang II, Schawel AK, Brown MJ, Krantz BA Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18378-83. doi:, 10.1073/pnas.1208280109. Epub 2012 Oct 24. PMID:23100533<ref>PMID:23100533</ref>
	~~[[http://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN]] One of the three proteins composing the anthrax~~ toxin, the ~~agent which infects many mammalian species and that may cause death~~. ~~PA binds to a receptor (ATR) in sensitive eukaryotic cells~~, ~~thereby facilitating the translocation of the enzymatic toxin components~~, ~~edema factor and lethal factor~~, ~~across the target cell membrane~~. ~~PA associated with LF causes death when injected~~, ~~PA associated with EF produces edema~~. ~~PA induces immunity to infection with anthrax~~.

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[4ee2]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_anthracis Bacillus anthracis]~~. ~~Full crystallographic information is available from [http:/~~/~~oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA].~~		</div>

	==~~Reference~~==		==See Also==
	~~<ref group~~=~~"xtra">PMID:023100533</ref>~~<references ~~group="xtra"~~/><~~references~~/>		*[[Anthrax protective antigen\|Anthrax protective antigen]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Kintzer, A F.]]		[[Category: Kintzer, A F]]
	[[Category: Krantz, B A.]]		[[Category: Krantz, B A]]
	[[Category: Anthrax toxin]]		[[Category: Anthrax toxin]]
	[[Category: Assembly]]		[[Category: Assembly]]

OCA at 09:03, 4 September 2013

2013-09-04T09:03:35Z

← Older revision		Revision as of 09:03, 4 September 2013
Line 1:		Line 1:
	~~[[Image:4ee2.jpg\|left\|200px]]~~

	{{STRUCTURE_4ee2\| PDB=4ee2 \| SCENE= }}		{{STRUCTURE_4ee2\| PDB=4ee2 \| SCENE= }}

	===Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution===		===Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution===
			{{ABSTRACT_PUBMED_23100533}}

			==Function==
			[[http://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN]] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.

	==About this Structure==		==About this Structure==
	[[4ee2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA].		[[4ee2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA].

			==Reference==
			<ref group="xtra">PMID:023100533</ref><references group="xtra"/><references/>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Kintzer, A F.]]		[[Category: Kintzer, A F.]]

OCA at 07:43, 24 October 2012

2012-10-24T07:43:59Z

← Older revision		Revision as of 07:43, 24 October 2012
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4ee2.jpg\|left\|200px]]

	~~The entry~~ 4ee2 ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_4ee2\| PDB=4ee2 \| SCENE= }}

	~~Authors: Kintzer, A.F., Krantz, B~~.A.		===Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution===

	~~Description: Crystal~~ Structure ~~of Anthrax Protective Antigen K446M Mutant to~~ 1.91-A ~~Resolution~~
			==About this Structure==
			[[4ee2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE2 OCA].
			[[Category: Bacillus anthracis]]
			[[Category: Kintzer, A F.]]
			[[Category: Krantz, B A.]]
			[[Category: Anthrax toxin]]
			[[Category: Assembly]]
			[[Category: Cell-binding]]
			[[Category: Channel formation]]
			[[Category: Protein translocation]]
			[[Category: Toxin]]
			[[Category: Transport protein]]

OCA at 18:54, 2 May 2012

2012-05-02T18:54:32Z

← Older revision		Revision as of 18:54, 2 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4ee2 is ON HOLD		The entry 4ee2 is ON HOLD until Paper Publication

	Authors: Kintzer, A.F., Krantz, B.A.		Authors: Kintzer, A.F., Krantz, B.A.

	Description: Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution		Description: Crystal Structure of Anthrax Protective Antigen K446M Mutant to 1.91-A Resolution

OCA: Protected "4ee2" [edit=sysop:move=sysop]

2012-04-04T05:50:00Z

Protected "4ee2" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:50, 4 April 2012
(No difference)