4eef - Revision history

OCA at 06:55, 27 November 2024

2024-11-27T06:55:50Z

← Older revision		Revision as of 06:55, 27 November 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Brevig_Mission/1/1918(H1N1)) Influenza A virus (A/Brevig Mission/1/1918(H1N1))] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Brevig_Mission/1/1918(H1N1)) Influenza A virus (A/Brevig Mission/1/1918(H1N1))] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EEF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.704Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [https://pdbe.org/4eef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [https://www.ebi.ac.uk/pdbsum/4eef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [https://pdbe.org/4eef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [https://www.ebi.ac.uk/pdbsum/4eef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eef ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/HEMA_I18A0 HEMA_I18A0]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).		[https://www.uniprot.org/uniprot/HEMA_I18A0 HEMA_I18A0] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:07, 28 September 2022

2022-09-28T07:07:39Z

← Older revision		Revision as of 07:07, 28 September 2022
Line 1:		Line 1:

	==Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.==		==Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.==
	<StructureSection load='4eef' size='340' side='right' caption='[[4eef]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4eef' size='340' side='right'caption='[[4eef]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~I18a0 I18a0~~] and [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Brevig_Mission/1/1918(H1N1)) Influenza A virus (A/Brevig Mission/1/1918(H1N1))] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EEF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA, hemagglutinin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=88776 I18A0])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [https://pdbe.org/4eef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [https://www.ebi.ac.uk/pdbsum/4eef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eef ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [~~http~~://pdbe.org/4eef PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eef PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eef ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/HEMA_I18A0 HEMA_I18A0]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).		[[https://www.uniprot.org/uniprot/HEMA_I18A0 HEMA_I18A0]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
	*[[Hemagglutinin\|Hemagglutinin]]		*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~I18a0~~]]		[[Category: Large Structures]]
	[[Category: Synthetic construct ~~sequences~~]]		[[Category: Synthetic construct]]
	[[Category: Dreyfus, C]]		[[Category: Dreyfus C]]
	[[Category: Wilson~~, I A]]~~		[[Category: Wilson IA]]
	~~[[Category: Fusion of virus membrane with host membrane]]~~
	~~[[Category: Hemagglutinin]]~~
	~~[[Category: Immune system]]~~
	~~[[Category: Immune system-inhibitor complex]]~~
	~~[[Category: Immunoglobulin]]~~
	~~[[Category: Membrane fusion]]~~
	~~[[Category: Sialic acid]]~~
	~~[[Category: Virion~~]]

OCA at 16:31, 4 August 2016

2016-08-04T16:31:13Z

← Older revision		Revision as of 16:31, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.==		==Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.==
	<StructureSection load='4eef' size='340' side='right' caption='[[4eef]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4eef' size='340' side='right' caption='[[4eef]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Influenza_a_virus Influenza a virus~~] and [http://en.wikipedia.org/wiki/~~Synthetic_construct~~ Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/I18a0 I18a0] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EEF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA, hemagglutinin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~11320 Influenza A virus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA, hemagglutinin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=88776 I18A0])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [http://www.ebi.ac.uk/pdbsum/4eef PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [http://pdbe.org/4eef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [http://www.ebi.ac.uk/pdbsum/4eef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eef ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eef" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Influenza a virus~~]]		[[Category: I18a0]]
	[[Category: Synthetic construct]]		[[Category: Synthetic construct sequences]]
	[[Category: Dreyfus, C]]		[[Category: Dreyfus, C]]
	[[Category: Wilson, I A]]		[[Category: Wilson, I A]]

OCA at 17:02, 24 December 2014

2014-12-24T17:02:56Z

← Older revision		Revision as of 17:02, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EEF FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA, hemagglutinin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 Influenza A virus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA, hemagglutinin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 Influenza A virus])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [http://www.ebi.ac.uk/pdbsum/4eef PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [http://www.ebi.ac.uk/pdbsum/4eef PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/HEMA_I18A0 HEMA_I18A0]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
	[[Category: Synthetic construct]]		[[Category: Synthetic construct]]
	[[Category: Dreyfus, C.]]		[[Category: Dreyfus, C]]
	[[Category: Wilson, I A.]]		[[Category: Wilson, I A]]
	[[Category: Fusion of virus membrane with host membrane]]		[[Category: Fusion of virus membrane with host membrane]]
	[[Category: Hemagglutinin]]		[[Category: Hemagglutinin]]

OCA at 08:19, 5 June 2014

2014-06-05T08:19:33Z

← Older revision		Revision as of 08:19, 5 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~4eef~~.~~jpg~~\|~~left~~\|~~200px~~]]		==Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.==
			<StructureSection load='4eef' size='340' side='right' caption='[[4eef]], [[Resolution\|resolution]] 2.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4eef]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EEF FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA, hemagglutinin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 Influenza A virus])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eef OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eef RCSB], [http://www.ebi.ac.uk/pdbsum/4eef PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			We show that comprehensive sequence-function maps obtained by deep sequencing can be used to reprogram interaction specificity and to leapfrog over bottlenecks in affinity maturation by combining many individually small contributions not detectable in conventional approaches. We use this approach to optimize two computationally designed inhibitors against H1N1 influenza hemagglutinin and, in both cases, obtain variants with subnanomolar binding affinity. The most potent of these, a 51-residue protein, is broadly cross-reactive against all influenza group 1 hemagglutinins, including human H2, and neutralizes H1N1 viruses with a potency that rivals that of several human monoclonal antibodies, demonstrating that computational design followed by comprehensive energy landscape mapping can generate proteins with potential therapeutic utility.

	~~{{STRUCTURE_4eef\| PDB=4eef \| SCENE= }}~~		Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing.,Whitehead TA, Chevalier A, Song Y, Dreyfus C, Fleishman SJ, De Mattos C, Myers CA, Kamisetty H, Blair P, Wilson IA, Baker D Nat Biotechnol. 2012 May 27;30(6):543-8. doi: 10.1038/nbt.2214. PMID:22634563<ref>PMID:22634563</ref>

	~~===Crystal structure~~ of the ~~designed inhibitor protein F-HB80~~.~~4 in complex with the 1918 influenza virus hemagglutinin~~.~~===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22634563}}~~		==See Also==
			*[[Hemagglutinin\|Hemagglutinin]]
	==~~About this Structure~~==		== References ==
	[[~~4eef]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEF OCA~~].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:022634563<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
	[[Category: Synthetic construct]]		[[Category: Synthetic construct]]

OCA at 05:43, 27 June 2012

2012-06-27T05:43:40Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4eef.jpg|left|200px]]
-The entry 4eef is ON HOLD  until Paper Publication
+{{STRUCTURE_4eef|  PDB=4eef  |  SCENE=  }}
-Authors: Dreyfus, C., Wilson, I.A.
+===Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.===
-Description: Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.
+{{ABSTRACT_PUBMED_22634563}}

OCA at 07:15, 6 June 2012

2012-06-06T07:15:39Z

← Older revision		Revision as of 07:15, 6 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eef is ON HOLD		The entry 4eef is ON HOLD until Paper Publication

	Authors: Dreyfus, C., Wilson, I.A.		Authors: Dreyfus, C., Wilson, I.A.

	Description: Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.		Description: Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.

OCA at 06:41, 11 April 2012

2012-04-11T06:41:14Z

← Older revision		Revision as of 06:41, 11 April 2012
Line 3:		Line 3:
	The entry 4eef is ON HOLD		The entry 4eef is ON HOLD

	Authors: Dreyfus, C., Wilson, I. A.		Authors: Dreyfus, C., Wilson, I.A.

	Description: Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.		Description: Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.

OCA: Protected "4eef" [edit=sysop:move=sysop]

2012-04-04T05:50:24Z

Protected "4eef" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:50, 4 April 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4eef is ON HOLD Authors: Dreyfus, C., Wilson, I. A. Description: Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1...

2012-04-04T05:50:23Z

New page: '''Unreleased structure''' The entry 4eef is ON HOLD Authors: Dreyfus, C., Wilson, I. A. Description: Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1...

New page

'''Unreleased structure'''

The entry 4eef is ON HOLD

Authors: Dreyfus, C., Wilson, I. A.

Description: Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.