4eep - Revision history

OCA at 14:59, 14 March 2024

2024-03-14T14:59:29Z

← Older revision		Revision as of 14:59, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eep]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EEP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eep]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EEP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eep OCA], [https://pdbe.org/4eep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eep RCSB], [https://www.ebi.ac.uk/pdbsum/4eep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eep ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eep OCA], [https://pdbe.org/4eep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eep RCSB], [https://www.ebi.ac.uk/pdbsum/4eep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eep ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PHOT2_ARATH PHOT2_ARATH]] Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Mediates calcium spiking of extra- and intracellular origins in response to blue light. Involved in hypocotyl phototropism. Contributes to the chloroplast accumulation in low blue light and mediates their translocation (avoidance response) at high fluence. Regulates stomata opening and photomorphogenesis response of leaf tissue. Not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.<ref>PMID:11371609</ref> <ref>PMID:11251116</ref> <ref>PMID:12821778</ref> <ref>PMID:15031408</ref> <ref>PMID:14982991</ref>		[https://www.uniprot.org/uniprot/PHOT2_ARATH PHOT2_ARATH] Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Mediates calcium spiking of extra- and intracellular origins in response to blue light. Involved in hypocotyl phototropism. Contributes to the chloroplast accumulation in low blue light and mediates their translocation (avoidance response) at high fluence. Regulates stomata opening and photomorphogenesis response of leaf tissue. Not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.<ref>PMID:11371609</ref> <ref>PMID:11251116</ref> <ref>PMID:12821778</ref> <ref>PMID:15031408</ref> <ref>PMID:14982991</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Fluorescent proteins derived from Light, Oxygen or Voltage (LOV) domains offer advantages over Green Fluorescent Protein (GFP) from their small size and efficacy under anaerobic conditions. The flavoprotein iLOV was engineered from the blue-light receptor phototropin as a reporter of viral infection. To inform the molecular basis for the improved, photoreversible, fluorescent properties of iLOV, we employed directed evolution and determined five LOV crystallographic structures. Comparative structural analyses between iLOV and its progenitors reveal mutation-induced constraints in the environment of the flavin mononcleotide (FMN) chromophore: in iLOV, the methyl group of Thr 394 crowds the FMN isoalloxazine ring, Leu470 triggers side-chain flipping of Leu472, and the terminal FMN phosphate shows increased anchoring. We further engineered iLOV variants that are readily detectable in bacterial and mammalian cells, due to order-of-magnitude photostability increases. Structure determination of a resulting representative photostable iLOV (phiLOV) variant reveals additional constraints on the chromophore. Aromatic residues Tyr401 and Phe485 in phiLOV sandwich the FMN isoalloxazine ring from both sides, while Ser390 anchors the side chain of FMN-interacting Gln489 Our combined structural and mutational results reveal that constraining the FMN fluorophore yields improved photochemical properties for iLOV and its new photostable derivative. These findings provide a framework for structural fine-tuning of LOV-scaffold proteins to maximize their potential as oxygen-independent fluorescent reporters.

	Structural Tuning of the Fluorescent Protein iLOV for Improved Photostability.,Christie JM, Hitomi K, Arvai AS, Hartfield KA, Mettlen M, Pratt AJ, Tainer JA, Getzoff ED J Biol Chem. 2012 May 9. PMID:22573334<ref>PMID:22573334</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4eep" style="background-color:#fffaf0;"></div~~>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:07, 28 September 2022

2022-09-28T07:07:54Z

← Older revision		Revision as of 07:07, 28 September 2022
Line 1:		Line 1:

	==Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2==		==Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2==
	<StructureSection load='4eep' size='340' side='right' caption='[[4eep]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4eep' size='340' side='right'caption='[[4eep]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eep]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. The March 2015 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EEP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eep]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EEP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eer\|4eer]], [[4ees\|4ees]], [[4eet\|4eet]], [[4eeu\|4eeu]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eep OCA], [https://pdbe.org/4eep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eep RCSB], [https://www.ebi.ac.uk/pdbsum/4eep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eep ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PHOT2, CAV1, KIN7, NPL1, At5g58140, K21L19.6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eep OCA], [~~http~~://pdbe.org/4eep PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eep RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eep PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eep ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PHOT2_ARATH PHOT2_ARATH]] Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Mediates calcium spiking of extra- and intracellular origins in response to blue light. Involved in hypocotyl phototropism. Contributes to the chloroplast accumulation in low blue light and mediates their translocation (avoidance response) at high fluence. Regulates stomata opening and photomorphogenesis response of leaf tissue. Not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.<ref>PMID:11371609</ref> <ref>PMID:11251116</ref> <ref>PMID:12821778</ref> <ref>PMID:15031408</ref> <ref>PMID:14982991</ref>		[[https://www.uniprot.org/uniprot/PHOT2_ARATH PHOT2_ARATH]] Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Mediates calcium spiking of extra- and intracellular origins in response to blue light. Involved in hypocotyl phototropism. Contributes to the chloroplast accumulation in low blue light and mediates their translocation (avoidance response) at high fluence. Regulates stomata opening and photomorphogenesis response of leaf tissue. Not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.<ref>PMID:11371609</ref> <ref>PMID:11251116</ref> <ref>PMID:12821778</ref> <ref>PMID:15031408</ref> <ref>PMID:14982991</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Arath~~]]		[[Category: Arabidopsis thaliana]]
	[[Category: ~~Non-specific serine/threonine protein kinase~~]]		[[Category: Large Structures]]
	[[Category: Phototropin]]		[[Category: Phototropin]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Arvai~~, A S~~]]		[[Category: Arvai AS]]
	[[Category: Christie~~, J M~~]]		[[Category: Christie JM]]
	[[Category: Getzoff~~, E D~~]]		[[Category: Getzoff ED]]
	[[Category: Hartfield~~, K A~~]]		[[Category: Hartfield KA]]
	[[Category: Hitomi, K]]		[[Category: Hitomi K]]
	[[Category: Pratt~~, A J~~]]		[[Category: Pratt AJ]]
	[[Category: Tainer~~, J A]]~~		[[Category: Tainer JA]]
	~~[[Category: Blue light photoreceptor]]~~
	~~[[Category: Flavoprotein]]~~
	~~[[Category: Lov]]~~
	~~[[Category: Signaling protein~~]]

OCA at 20:21, 4 August 2016

2016-08-04T20:21:57Z

← Older revision		Revision as of 20:21, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2==		==Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2==
	<StructureSection load='4eep' size='340' side='right' caption='[[4eep]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4eep' size='340' side='right' caption='[[4eep]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eep]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EEP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eep]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. The March 2015 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EEP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eer\|4eer]], [[4ees\|4ees]], [[4eet\|4eet]], [[4eeu\|4eeu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eer\|4eer]], [[4ees\|4ees]], [[4eet\|4eet]], [[4eeu\|4eeu]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PHOT2, CAV1, KIN7, NPL1, At5g58140, K21L19.6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PHOT2, CAV1, KIN7, NPL1, At5g58140, K21L19.6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eep OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eep RCSB], [http://www.ebi.ac.uk/pdbsum/4eep PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eep OCA], [http://pdbe.org/4eep PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eep RCSB], [http://www.ebi.ac.uk/pdbsum/4eep PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eep ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eep" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Arabidopsis thaliana~~]]		[[Category: Arath]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
			[[Category: Phototropin]]
			[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Arvai, A S]]		[[Category: Arvai, A S]]
	[[Category: Christie, J M]]		[[Category: Christie, J M]]

OCA at 12:50, 25 December 2014

2014-12-25T12:50:03Z

← Older revision		Revision as of 12:50, 25 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~4eep~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2==
			<StructureSection load='4eep' size='340' side='right' caption='[[4eep]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4eep]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EEP FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eer\|4eer]], [[4ees\|4ees]], [[4eet\|4eet]], [[4eeu\|4eeu]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PHOT2, CAV1, KIN7, NPL1, At5g58140, K21L19.6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eep OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eep RCSB], [http://www.ebi.ac.uk/pdbsum/4eep PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PHOT2_ARATH PHOT2_ARATH]] Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Mediates calcium spiking of extra- and intracellular origins in response to blue light. Involved in hypocotyl phototropism. Contributes to the chloroplast accumulation in low blue light and mediates their translocation (avoidance response) at high fluence. Regulates stomata opening and photomorphogenesis response of leaf tissue. Not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.<ref>PMID:11371609</ref> <ref>PMID:11251116</ref> <ref>PMID:12821778</ref> <ref>PMID:15031408</ref> <ref>PMID:14982991</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Fluorescent proteins derived from Light, Oxygen or Voltage (LOV) domains offer advantages over Green Fluorescent Protein (GFP) from their small size and efficacy under anaerobic conditions. The flavoprotein iLOV was engineered from the blue-light receptor phototropin as a reporter of viral infection. To inform the molecular basis for the improved, photoreversible, fluorescent properties of iLOV, we employed directed evolution and determined five LOV crystallographic structures. Comparative structural analyses between iLOV and its progenitors reveal mutation-induced constraints in the environment of the flavin mononcleotide (FMN) chromophore: in iLOV, the methyl group of Thr 394 crowds the FMN isoalloxazine ring, Leu470 triggers side-chain flipping of Leu472, and the terminal FMN phosphate shows increased anchoring. We further engineered iLOV variants that are readily detectable in bacterial and mammalian cells, due to order-of-magnitude photostability increases. Structure determination of a resulting representative photostable iLOV (phiLOV) variant reveals additional constraints on the chromophore. Aromatic residues Tyr401 and Phe485 in phiLOV sandwich the FMN isoalloxazine ring from both sides, while Ser390 anchors the side chain of FMN-interacting Gln489 Our combined structural and mutational results reveal that constraining the FMN fluorophore yields improved photochemical properties for iLOV and its new photostable derivative. These findings provide a framework for structural fine-tuning of LOV-scaffold proteins to maximize their potential as oxygen-independent fluorescent reporters.

	~~{{STRUCTURE_4eep\| PDB=4eep \| SCENE= }}~~		Structural Tuning of the Fluorescent Protein iLOV for Improved Photostability.,Christie JM, Hitomi K, Arvai AS, Hartfield KA, Mettlen M, Pratt AJ, Tainer JA, Getzoff ED J Biol Chem. 2012 May 9. PMID:22573334<ref>PMID:22573334</ref>

	~~===Crystal structure of LOV2 domain~~ of ~~Arabidopsis thaliana phototropin 2===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22573334}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[4eep]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:022573334<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Arvai, A S.]]		[[Category: Arvai, A S]]
	[[Category: Christie, J M.]]		[[Category: Christie, J M]]
	[[Category: Getzoff, E D.]]		[[Category: Getzoff, E D]]
	[[Category: Hartfield, K A.]]		[[Category: Hartfield, K A]]
	[[Category: Hitomi, K]]		[[Category: Hitomi, K]]
	[[Category: Pratt, A J.]]		[[Category: Pratt, A J]]
	[[Category: Tainer, J A.]]		[[Category: Tainer, J A]]
	[[Category: Blue light photoreceptor]]		[[Category: Blue light photoreceptor]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]
	[[Category: Lov]]		[[Category: Lov]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 07:27, 11 July 2012

2012-07-11T07:27:13Z

← Older revision		Revision as of 07:27, 11 July 2012
Line 1:		Line 1:
	[[Image:4eep.png\|left\|200px]]		[[Image:4eep.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_4eep", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_4eep\| PDB=4eep \| SCENE= }}		{{STRUCTURE_4eep\| PDB=4eep \| SCENE= }}

	===Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2===		===Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_22573334}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 22573334 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_22573334}}		{{ABSTRACT_PUBMED_22573334}}

OCA at 06:43, 6 June 2012

2012-06-06T06:43:57Z

← Older revision		Revision as of 06:43, 6 June 2012
Line 1:		Line 1:
	[[Image:4eep.~~jpg~~\|left\|200px]]		[[Image:4eep.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2===		===Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_22573334}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 22573334 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_22573334}}

	==About this Structure==		==About this Structure==
	[[4eep]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA].		[[4eep]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EEP OCA].

			==Reference==
			<ref group="xtra">PMID:022573334</ref><references group="xtra"/>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]

OCA at 09:24, 16 May 2012

2012-05-16T09:24:07Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4eep.jpg|left|200px]]
-The entry 4eep is ON HOLD  until Paper Publication
+<!--
-Authors: Hitomi, K, Christie, J.M., Arvai, A.S., Hartfield, K.A., Pratt, A.J., Tainer, J.A., Getzoff, E.D.
+===Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2===
-Description: Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2

OCA at 08:46, 9 May 2012

2012-05-09T08:46:25Z

← Older revision		Revision as of 08:46, 9 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eep is ON HOLD		The entry 4eep is ON HOLD until Paper Publication

	Authors: Hitomi, K, Christie, J.M., Arvai, A.S., Hartfield, K.A., Pratt, A.J., Tainer, J.A., Getzoff, E.D.		Authors: Hitomi, K, Christie, J.M., Arvai, A.S., Hartfield, K.A., Pratt, A.J., Tainer, J.A., Getzoff, E.D.

	Description: Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2		Description: Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2

OCA at 06:41, 11 April 2012

2012-04-11T06:41:20Z

← Older revision		Revision as of 06:41, 11 April 2012
Line 3:		Line 3:
	The entry 4eep is ON HOLD		The entry 4eep is ON HOLD

	Authors: Hitomi, K, Christie, J.M., Arvai, A.S., Hartfield, K.A., Pratt, A.J. , Tainer, J.A., Getzoff, E.D.		Authors: Hitomi, K, Christie, J.M., Arvai, A.S., Hartfield, K.A., Pratt, A.J., Tainer, J.A., Getzoff, E.D.

	Description: Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2		Description: Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2

OCA: Protected "4eep" [edit=sysop:move=sysop]

2012-04-04T05:50:42Z

Protected "4eep" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:50, 4 April 2012
(No difference)