4ehs - Revision history

OCA at 22:52, 26 March 2025

2025-03-26T22:52:26Z

← Older revision		Revision as of 22:52, 26 March 2025
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ehs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EHS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ehs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EHS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.78Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ehs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehs OCA], [https://pdbe.org/4ehs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ehs RCSB], [https://www.ebi.ac.uk/pdbsum/4ehs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ehs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehs OCA], [https://pdbe.org/4ehs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ehs RCSB], [https://www.ebi.ac.uk/pdbsum/4ehs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/DNAG_HELPY DNAG_HELPY]] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.[HAMAP-Rule:MF_00974]		[https://www.uniprot.org/uniprot/DNAG_HELPY DNAG_HELPY] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stimulates the 5'-3' DNA helicase activity of DnaB (PubMed:23585534, PubMed:26264665).[HAMAP-Rule:MF_00974]<ref>PMID:23585534</ref> <ref>PMID:26264665</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:12, 28 September 2022

2022-09-28T07:12:02Z

← Older revision		Revision as of 07:12, 28 September 2022
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	==Crystal structure of Helicobacter pylori DnaG Primase C terminal domain==		==Crystal structure of Helicobacter pylori DnaG Primase C terminal domain==
	<StructureSection load='4ehs' size='340' side='right' caption='[[4ehs]], [[Resolution\|resolution]] 1.78Å' scene=''>		<StructureSection load='4ehs' size='340' side='right'caption='[[4ehs]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ehs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Campylobacter_pylori Campylobacter~~ pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EHS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ehs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EHS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ehs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehs OCA], [https://pdbe.org/4ehs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ehs RCSB], [https://www.ebi.ac.uk/pdbsum/4ehs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehs ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaG, DnaG Primase, HP_0012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ehs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehs OCA], [~~http~~://pdbe.org/4ehs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ehs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ehs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehs ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/DNAG_HELPY DNAG_HELPY]] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.[HAMAP-Rule:MF_00974]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4ehs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4ehs" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Campylobacter~~ pylori]]		[[Category: Helicobacter pylori 26695]]
	[[Category: ~~Gourinath, S~~]]		[[Category: Large Structures]]
	[[Category: Rehman~~, S A.Abdul~~]]		[[Category: Abdul Rehman SA]]
	[[Category: ~~Helicase binding domain]]~~		[[Category: Gourinath S]]
	~~[[Category: Primase]]~~
	~~[[Category: Transferase~~]]

OCA at 08:04, 15 November 2017

2017-11-15T08:04:32Z

← Older revision		Revision as of 08:04, 15 November 2017
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	</div>		</div>
	<div class="pdbe-citations 4ehs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4ehs" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[RNA polymerase\|RNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:11, 11 August 2016

2016-08-11T17:11:12Z

← Older revision		Revision as of 17:11, 11 August 2016
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	==Crystal structure of Helicobacter pylori DnaG Primase C terminal domain==		==Crystal structure of Helicobacter pylori DnaG Primase C terminal domain==
	<StructureSection load='4ehs' size='340' side='right' caption='[[4ehs]], [[Resolution\|resolution]] 1.78Å' scene=''>		<StructureSection load='4ehs' size='340' side='right' caption='[[4ehs]], [[Resolution\|resolution]] 1.78Å' scene=''>
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaG, DnaG Primase, HP_0012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaG, DnaG Primase, HP_0012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ehs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ehs RCSB], [http://www.ebi.ac.uk/pdbsum/4ehs PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ehs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehs OCA], [http://pdbe.org/4ehs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ehs RCSB], [http://www.ebi.ac.uk/pdbsum/4ehs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4ehs" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 14:02, 4 January 2015

2015-01-04T14:02:25Z

← Older revision		Revision as of 14:02, 4 January 2015
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	~~{{STRUCTURE_4ehs\| PDB~~=4ehs \| ~~SCENE~~= }}		==Crystal structure of Helicobacter pylori DnaG Primase C terminal domain==
	===~~Crystal~~ structure ~~of Helicobacter~~ pylori DnaG Primase ~~C terminal domain~~===		<StructureSection load='4ehs' size='340' side='right' caption='[[4ehs]], [[Resolution\|resolution]] 1.78Å' scene=''>
	~~{{ABSTRACT_PUBMED_23585534}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4ehs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EHS FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
			<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaG, DnaG Primase, HP_0012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ehs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ehs RCSB], [http://www.ebi.ac.uk/pdbsum/4ehs PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			To better understand the poor conservation of helicase binding domain of primases/DnaG's among the eubacteria, we have determined the crystal structure of the Helicobacter pylori DnaG-CTD at 1.78 A. The structure has a globular subdomain connected to a helical hairpin. Structural comparison has revealed that globular subdomains despite the variation in number of helices have broadly similar arrangements across the species, whereas helical hairpins show different orientations. Further, to study the helicase-primase interaction in H. pylori (Hp), a complex was modeled using the HpDnaG-CTD and HpDnaB-NTD (helicase) crystal structures with the Bacillus stearothermophilus BstDnaB-BstDnaG-CTD (helicase-primase) complex structure as a template. Through the model a non-conserved critical residue Phe534 on helicase binding interface of DnaG-CTD was identified. Mutation guided by molecular dynamics, biophysical and biochemical studies validated our model. We have further concluded that species specific helicase-primase interactions are influenced by electrostatic surface potentials apart from the critical hydrophobic surface residues.

	~~==About this Structure==~~		Crystal structure and mode of helicase binding of the C-terminal domain of primase from Helicobacter pylori.,Abdul Rehman SA, Verma V, Mazumder M, Dhar SK, Gourinath S J Bacteriol. 2013 Apr 12. PMID:23585534<ref>PMID:23585534</ref>
	~~[[4ehs]] is a 2 chain~~ structure ~~with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Campylobacter_pylori Campylobacter pylori]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group~~=~~"xtra">PMID:023585534</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>

			==See Also==
			*[[RNA polymerase\|RNA polymerase]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Campylobacter pylori]]		[[Category: Campylobacter pylori]]
	[[Category: Gourinath, S.]]		[[Category: Gourinath, S]]
	[[Category: Rehman, S A.Abdul.]]		[[Category: Rehman, S A.Abdul]]
	[[Category: Helicase binding domain]]		[[Category: Helicase binding domain]]
	[[Category: Primase]]		[[Category: Primase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:24, 12 March 2014

2014-03-12T14:24:13Z

← Older revision		Revision as of 14:24, 12 March 2014
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	==About this Structure==		==About this Structure==
	[[4ehs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Helicobacter_pylori_26695 Helicobacter~~ pylori ~~26695~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA].		[[4ehs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:023585534</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:023585534</ref><references group="xtra"/><references/>
	[[Category: ~~Helicobacter~~ pylori ~~26695~~]]		[[Category: Campylobacter pylori]]
	[[Category: Gourinath, S.]]		[[Category: Gourinath, S.]]
	[[Category: Rehman, S A.Abdul.]]		[[Category: Rehman, S A.Abdul.]]

OCA at 06:33, 20 June 2013

2013-06-20T06:33:41Z

← Older revision		Revision as of 06:33, 20 June 2013
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	==About this Structure==		==About this Structure==
	[[4ehs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA].		[[4ehs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA].

			==Reference==
			<ref group="xtra">PMID:023585534</ref><references group="xtra"/><references/>
	[[Category: Helicobacter pylori 26695]]		[[Category: Helicobacter pylori 26695]]
	[[Category: Gourinath, S.]]		[[Category: Gourinath, S.]]

OCA at 07:42, 2 May 2013

2013-05-02T07:42:24Z

← Older revision		Revision as of 07:42, 2 May 2013
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	~~'''Unreleased~~ structure~~'''~~		{{STRUCTURE_4ehs\| PDB=4ehs \| SCENE= }}
			===Crystal structure of Helicobacter pylori DnaG Primase C terminal domain===
			{{ABSTRACT_PUBMED_23585534}}

	~~The entry~~ 4ehs is ~~ON HOLD until Paper Publication~~		==About this Structure==
			[[4ehs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHS OCA].
	~~Authors~~: ~~Abdul~~ Rehman, S.A.~~, Gourinath, S~~.		[[Category: Helicobacter pylori 26695]]
			[[Category: Gourinath, S.]]
	~~Description~~: ~~Crystal structure of Helicobacter pylori DnaG~~ Primase ~~C terminal domain~~		[[Category: Rehman, S A.Abdul.]]
			[[Category: Helicase binding domain]]
			[[Category: Primase]]
			[[Category: Transferase]]

OCA at 07:15, 6 June 2012

2012-06-06T07:15:56Z

← Older revision		Revision as of 07:15, 6 June 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4ehs is ON HOLD		The entry 4ehs is ON HOLD until Paper Publication

	Authors: Abdul Rehman, S.A., Gourinath, S.		Authors: Abdul Rehman, S.A., Gourinath, S.

	Description: Crystal structure of Helicobacter pylori DnaG Primase C terminal domain		Description: Crystal structure of Helicobacter pylori DnaG Primase C terminal domain

OCA at 08:46, 9 May 2012

2012-05-09T08:46:54Z

← Older revision		Revision as of 08:46, 9 May 2012
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	The entry 4ehs is ON HOLD		The entry 4ehs is ON HOLD

	Authors: Abdul Rehman,S.A., Gourinath, S.		Authors: Abdul Rehman, S.A., Gourinath, S.

	Description: Crystal structure of Helicobacter pylori DnaG Primase C terminal domain		Description: Crystal structure of Helicobacter pylori DnaG Primase C terminal domain