4eib - Revision history

OCA at 13:47, 8 November 2023

2023-11-08T13:47:48Z

← Older revision		Revision as of 13:47, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eib]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizopus_arrhizus Rhizopus arrhizus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eib]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizopus_arrhizus Rhizopus arrhizus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.86Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eib OCA], [https://pdbe.org/4eib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eib RCSB], [https://www.ebi.ac.uk/pdbsum/4eib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eib ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eib OCA], [https://pdbe.org/4eib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eib RCSB], [https://www.ebi.ac.uk/pdbsum/4eib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eib ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/B7XC04_RHIOR B7XC04_RHIOR]]		[https://www.uniprot.org/uniprot/B7XC04_RHIOR B7XC04_RHIOR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:12, 28 September 2022

2022-09-28T07:12:44Z

← Older revision		Revision as of 07:12, 28 September 2022
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	==Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.==		==Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.==
	<StructureSection load='4eib' size='340' side='right' caption='[[4eib]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='4eib' size='340' side='right'caption='[[4eib]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eib]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_56536 Atcc 56536~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EIB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eib]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizopus_arrhizus Rhizopus arrhizus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amyA, CBM21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64495 ATCC 56536])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eib OCA], [https://pdbe.org/4eib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eib RCSB], [https://www.ebi.ac.uk/pdbsum/4eib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eib ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eib OCA], [~~http~~://pdbe.org/4eib PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eib RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eib PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eib ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/B7XC04_RHIOR B7XC04_RHIOR]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Atcc 56536~~]]		[[Category: Large Structures]]
	[[Category: ~~Cheng, K C~~]]		[[Category: Rhizopus arrhizus]]
	[[Category: ~~Lyu, P C~~]]		[[Category: Cheng KC]]
	[[Category: ~~Stephen, P~~]]		[[Category: Lyu PC]]
	[[Category: ~~Amylose]]~~		[[Category: Stephen P]]
	~~[[Category: Beta barrel]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Starch binding~~]]

OCA at 00:58, 5 August 2016

2016-08-05T00:58:59Z

← Older revision		Revision as of 00:58, 5 August 2016
Line 1:		Line 1:

	==Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.==		==Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.==
	<StructureSection load='4eib' size='340' side='right' caption='[[4eib]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='4eib' size='340' side='right' caption='[[4eib]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eib]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhizopus_oryzae Rhizopus oryzae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eib]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56536 Atcc 56536]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amyA, CBM21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64495 ~~Rhizopus oryzae~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amyA, CBM21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64495 ATCC 56536])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eib OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eib RCSB], [http://www.ebi.ac.uk/pdbsum/4eib PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eib OCA], [http://pdbe.org/4eib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eib RCSB], [http://www.ebi.ac.uk/pdbsum/4eib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eib ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eib" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Rhizopus oryzae~~]]		[[Category: Atcc 56536]]
	[[Category: Cheng, K C]]		[[Category: Cheng, K C]]
	[[Category: Lyu, P C]]		[[Category: Lyu, P C]]

OCA at 11:45, 21 December 2014

2014-12-21T11:45:05Z

← Older revision		Revision as of 11:45, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4eib\| PDB=4eib \| SCENE= }}~~		==Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.==
	===Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.===		<StructureSection load='4eib' size='340' side='right' caption='[[4eib]], [[Resolution\|resolution]] 1.86Å' scene=''>
	~~{{ABSTRACT_PUBMED_23226294}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4eib]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizopus_oryzae Rhizopus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIB FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amyA, CBM21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64495 Rhizopus oryzae])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eib OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eib RCSB], [http://www.ebi.ac.uk/pdbsum/4eib PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Glucoamylases, containing starch-binding domains (SBD), have a wide range of scientific and industrial applications. Random mutagenesis and DNA shuffling of the gene encoding a starch-binding domain have resulted in only minor improvements in the affinities of the corresponding protein to their ligands, whereas circular permutation of the RoCBM21 substantially improved its binding affinity and selectivity towards longer-chain carbohydrates. For the study reported herein, we used a standard soluble ligand (amylose EX-I) to characterize the functional and structural aspects of circularly permuted RoCBM21 (CP90). Site-directed mutagenesis and the analysis of crystal structure reveal the dimerisation and an altered binding path, which may be responsible for improved affinity and altered selectivity of this newly created starch-binding domain. The functional and structural characterization of CP90 suggests that it has significant potential in industrial applications.

	~~==About this Structure==~~		Crystal structure of circular permuted RoCBM21 (CP90): dimerisation and proximity of binding sites.,Stephen P, Cheng KC, Lyu PC PLoS One. 2012;7(11):e50488. doi: 10.1371/journal.pone.0050488. Epub 2012 Nov 30. PMID:23226294<ref>PMID:23226294</ref>
	~~[[4eib]] is a 2 chain~~ structure ~~with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Rhizopus_oryzae Rhizopus oryzae]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4EIB OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:023226294</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Rhizopus oryzae]]		[[Category: Rhizopus oryzae]]
	[[Category: Cheng, K C.]]		[[Category: Cheng, K C]]
	[[Category: Lyu, P C.]]		[[Category: Lyu, P C]]
	[[Category: Stephen, P.]]		[[Category: Stephen, P]]
	[[Category: Amylose]]		[[Category: Amylose]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Starch binding]]		[[Category: Starch binding]]

OCA at 11:36, 24 July 2013

2013-07-24T11:36:47Z

← Older revision		Revision as of 11:36, 24 July 2013
Line 1:		Line 1:
	~~[[Image:4eib.jpg\|left\|200px]]~~

	{{STRUCTURE_4eib\| PDB=4eib \| SCENE= }}		{{STRUCTURE_4eib\| PDB=4eib \| SCENE= }}

	===Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.===		===Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.===
			{{ABSTRACT_PUBMED_23226294}}

	==About this Structure==		==About this Structure==
	[[4eib]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizopus_oryzae Rhizopus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA].		[[4eib]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizopus_oryzae Rhizopus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA].

			==Reference==
			<ref group="xtra">PMID:023226294</ref><references group="xtra"/><references/>
	[[Category: Rhizopus oryzae]]		[[Category: Rhizopus oryzae]]
	[[Category: Cheng, K C.]]		[[Category: Cheng, K C.]]

OCA at 21:25, 12 December 2012

2012-12-12T21:25:27Z

← Older revision		Revision as of 21:25, 12 December 2012
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4eib.jpg\|left\|200px]]

	~~The entry~~ 4eib ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_4eib\| PDB=4eib \| SCENE= }}

	~~Authors: Stephen, P., Cheng, K.C., Lyu, P.C~~.		===Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.===

	~~Description~~: ~~Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding~~.
			==About this Structure==
			[[4eib]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizopus_oryzae Rhizopus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIB OCA].
			[[Category: Rhizopus oryzae]]
			[[Category: Cheng, K C.]]
			[[Category: Lyu, P C.]]
			[[Category: Stephen, P.]]
			[[Category: Amylose]]
			[[Category: Beta barrel]]
			[[Category: Hydrolase]]
			[[Category: Starch binding]]

OCA at 08:47, 9 May 2012

2012-05-09T08:47:01Z

← Older revision		Revision as of 08:47, 9 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eib is ON HOLD		The entry 4eib is ON HOLD until Paper Publication

	Authors: Stephen, P., Cheng, K.C., Lyu, P.C.		Authors: Stephen, P., Cheng, K.C., Lyu, P.C.

	Description: Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.		Description: Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.

OCA: Protected "4eib" [edit=sysop:move=sysop]

2012-04-11T06:44:08Z

Protected "4eib" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:44, 11 April 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4eib is ON HOLD Authors: Stephen, P., Cheng, K.C., Lyu, P.C. Description: Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into th...

2012-04-11T06:44:07Z

New page: '''Unreleased structure''' The entry 4eib is ON HOLD Authors: Stephen, P., Cheng, K.C., Lyu, P.C. Description: Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into th...

New page

'''Unreleased structure'''

The entry 4eib is ON HOLD

Authors: Stephen, P., Cheng, K.C., Lyu, P.C.

Description: Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.